pycom13g27750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002049
Physical Location & Seq
Forward (+)
23885405 .. 23888922
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27750.21

Sequence Viewer

Length: 2448 bp
ATGACGAAGCATGGAAACATGGAACACATCATGCACTTTAGGCAACTCTGGAGGCAACTCAAGCCTATAAGCAACTTCACTGACTCTCTCGGTGATCACATACGCGTACCACACCTCTCCACAGTGAAAGCTTTAAAAATACCCAATCACCCACTTTATACACTCTGTCAGTGGTATGTTTATCTGCTAAGCTCTTCTGTCGATCTTGGGCCTCTTCTCCACTATCTTAGGGCCCTCCAAAACCCTCTCGCCAACGTCAAACCAACACAACGGTGTACGACAAGACCTGCCATAAAGTGCCACAAATGGTGCCATACCAATACTTGTATGAAAACTATTGTTGTAGGCAAATTCCATCAAATTCAACCGCTTATGCCATGCTCCTCTCTGATTGTCCATCTGTCTGAGGATGATATGTCGTACTGTAAAGTAATCTCGAACCAAGAGCTTCTTGGAATACTAACTGGAACTCCATGGTACTTCACAATCTTCGATATAAATAATTCAGCTAATCGTAGGTCGATCAACTATCACCTAAATGCCGTCATAACCATTATGTGTACAAGGAAGCTTGTACACAAAATCCACAGTAATGTTTTCCCATTTCCATTGTGGAACGAGAAGTGGCTGCATCAACCAAAAAGGCTTCTTCCTTTCTGCTTTAACTTGCTGACAAATGGCACATCTACTCACATACTCAGCAATTTTTCTTTTCATACACGGCCAATAATAAAATGGCCGAATGCCGAACAGTGTGCTTCATCCAAAATTTCTTTCTTTAATTCCGCATTATTTGACACGTACATTCTGTTCTCTTGCATAAGCATGCCATTAGTCTCCCGAACTCTGAGATCTTTCTTTTTCCCTAACTTTTTGCTTCAATTAACTCCTAGATTTCTTAACGAGCGGTGTCACCCTGATAATGGTAAGCACCACCACGACTCATCTAAACCTCCTGTTCCCACCAGGATGCTGGTTCTTGACCTAAGAACCATGTATTTGCATCACTCGAAAAGTCTTTTTAAGATGCTCGAGCCATCGCTCTACTCCTTCATTCTTCAAAGGAGTCCTCTGAGGAGGGTGAAGCGAAGACTGAATCGCATGAGCTATAGCGGCATAATTCTGACAGAAGACAACAAAATAATTAGAATATTCACCAAATATGCAGGACTCAGGGCGTGCTGGTTGTCACGTGAGAGTGAAATACGAGTGGATAAAAACCAACTACTAAAAGTACTAGCTAAAAAAAAACTAATTACACCACCCAAAGGTGACCATACAATGGTGATAATCTGTCAGATCTGCCGTGGATTCATCGCAAGCCACCAAAATGTGAGTGGGCAAAAACAAAGCTTTTCAAAACCATTTCAATTACCAAAGATAGTAACCCTTCGCCGTAAAACCTGTATAATTTCCCAGAAAATAGAATACATATTTATACCACAACCATGCTCAGAATACCAGGTGATATCAATCAATGCAAATGATATGTCAGCCGGACCGGAGTCACCTAATATGACCTATATGGCTCAAATCCACATCTTAACAAATATACTTGTACATGAGTCGGAACCACCTAAAGTGTGCTACGATCACGTGAAGATTGTGCGAATAATCGCGGGTCACCTACGAGTCGGAACCACTTATAGTGGTCTGTACGACAGGACTGTGCACCTAACTTGGATCCAAGGTGAGCGTATGGTGCAGGAGGTGAACATCACGTTTATGAGCTCTCAACACATCTCACATAATCATCAATTTACATTAACAGTCTACAACTCACCTGGTACTTACCTGAGCATCCATCGCGTCAAATCACAATATAAGCATGGCATTTCAAACATAATTTCATTTAAATCGATTTTCTGGGAAAAATCTCAAGTATATAGGTATATAGGGAAAACCAAATGCCCACTCACTAGTATGTGGAAGGGTCATAGCCCCCAAGCCTCGATTGACTGTGCTCGTCCTGAGGATAGGTCTCACCTATATACGAAACAACTATATAAACCTACTCAACACCACAAACATCCCCATATTTTTAGAAAAATTTTCCGACCACCCACGCGCCGGCCAAGCACAGGCACACGTACCAAAACCCTAACGGCATTAGGGAATATTCCGTTATATATACCTGACGCCATTAGTAATGTTGTTAGTATATTCCAGCTGCCGTCGGTCGCCGTCTGCATCCAGGAAACCGGAAAAACTTCTAATTCTCAAATTAGAGCTTACAATGAGAAGAAAAAAACCATACAATTTCAAGCCCTGAAATCCATGGGATTTCGCTGGAGAAGCCTCGATAATCCGGCCAAACTTGAAACTCACCAAACTCAACTTCCCGACGTCCAAAACACTTCGTTATTCTTCTCCAAGCTTCATGAAGACGTTTTAAAGCTTCCTAGTAGCTTAAAATCTCCTCAAAACTTCACATTTACGATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

816

Amino Acids

94.33

Weight (kDa)

9.92

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2349
Acc36I ACCTGC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 309
AccB7I CCANNNNNTGG 1 cut(s) 1282
AccBSI CCGCTC 1 cut(s) 907
AccI GTMKAC 1 cut(s) 1773
AccII CGCG 4 cut(s) 105, 1619, 1809, 2068
AciI CCGC 5 cut(s) 368, 786, 907, 1113, 1619
AclWI GGATC 2 cut(s) 1678, 1691
AcoI YGGCCR 4 cut(s) 722, 737, 2072, 2310
AcsI RAATTY 4 cut(s) 350, 360, 768, 2049
AcvI CACGTG 2 cut(s) 1193, 1597
AcyI GRCGYC 2 cut(s) 2139, 2346
AfiI CCNNNNNNNGG 5 cut(s) 923, 1268, 1282, 2070, 2081
AflIII ACRYGT 3 cut(s) 103, 798, 2087
AgsI TTSAA 8 cut(s) 365, 881, 1061, 1359, 1370, 1839, 2264, 2321
AhlI ACTAGT 1 cut(s) 1919
AjnI CCWGG 4 cut(s) 965, 1461, 1783, 2193
AjuI GAANNNNNNNTTGG 4 cut(s) 758, 790, 2322, 2354
AleI CACNNNNGTG 1 cut(s) 271
Alw21I GWGCWC 3 cut(s) 1674, 1733, 1966
Alw26I GTCTC 2 cut(s) 841, 1986
Alw44I GTGCAC 1 cut(s) 1670
AlwI GGATC 2 cut(s) 1678, 1691
Ama87I CYCGRG 1 cut(s) 1031
AoxI GGCC 6 cut(s) 209, 231, 722, 737, 2072, 2310
ApaI GGGCCC 1 cut(s) 235
ApaLI GTGCAC 1 cut(s) 1670
ApeKI GCWGC 2 cut(s) 628, 2170
ApoI RAATTY 4 cut(s) 350, 360, 768, 2049
ArsI GACNNNNNNTTYG 2 cut(s) 1475, 1507
Asp700I GAANNNNTTC 1 cut(s) 2209
AspLEI GCGC 1 cut(s) 2070
AspS9I GGNCC 4 cut(s) 209, 231, 232, 1499
AvaI CYCGRG 1 cut(s) 1031
AvaII GGWCC 1 cut(s) 1499
AxyI CCTNAGG 1 cut(s) 1971
BaeGI GKGCMC 2 cut(s) 235, 1674
BamHI GGATCC 1 cut(s) 1683
BanI GGYRCC 1 cut(s) 309
BanII GRGCYC 2 cut(s) 235, 1733
BbrPI CACGTG 2 cut(s) 1193, 1597
BbsI GAAGAC 3 cut(s) 1096, 1137, 2391
Bbv12I GWGCWC 3 cut(s) 1674, 1733, 1966
BbvI GCAGC 2 cut(s) 615, 2157
BccI CCATC 4 cut(s) 363, 405, 1045, 1812
BceAI ACGGC 7 cut(s) 527, 737, 1290, 1380, 2121, 2158, 2168
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciT130I CCWGG 4 cut(s) 967, 1463, 1785, 2195
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 841, 1986
BcuI ACTAGT 1 cut(s) 1919
BfaI CTAG 4 cut(s) 891, 1238, 1920, 2403
BfmI CTRYAG 1 cut(s) 1108
BfuAI ACCTGC 1 cut(s) 295
BglII AGATCT 2 cut(s) 851, 1299
BisI GCNGC 3 cut(s) 629, 1114, 2171
BlpI GCTNAGC 1 cut(s) 188
BlsI GCNGC 3 cut(s) 630, 1115, 2172
BmcAI AGTACT 1 cut(s) 1236
Bme1390I CCNGG 4 cut(s) 967, 1463, 1785, 2195
Bme18I GGWCC 1 cut(s) 1499
BmeT110I CYCGRG 1 cut(s) 1031
BmgT120I GGNCC 4 cut(s) 209, 231, 232, 1499
BmiI GGNNCC 5 cut(s) 233, 311, 1572, 1639, 1685
BmrFI CCNGG 4 cut(s) 967, 1463, 1785, 2195
BmsI GCATC 6 cut(s) 640, 960, 1012, 1017, 1809, 2199
BoxI GACNNNNGTC 1 cut(s) 1504
BpiI GAAGAC 3 cut(s) 1096, 1137, 2391
BpmI CTGGAG 2 cut(s) 70, 2311
Bpu10I CCTNAGC 1 cut(s) 1796
Bpu1102I GCTNAGC 1 cut(s) 188
BpuEI CTTGAG 2 cut(s) 44, 1863
Bsa29I ATCGAT 1 cut(s) 1859
BsaAI YACGTR 4 cut(s) 801, 1193, 1597, 2090
BsaBI GATNNNNATC 1 cut(s) 1472
BsaHI GRCGYC 2 cut(s) 2139, 2346
BsaI GGTCTC 1 cut(s) 1986
BsaJI CCNNGG 4 cut(s) 473, 1306, 1687, 2277
BsaWI WCCGGW 2 cut(s) 1501, 2201
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 5 cut(s) 923, 1268, 1282, 2070, 2081
Bse118I RCCGGY 1 cut(s) 2070
Bse1I ACTGG 1 cut(s) 469
Bse21I CCTNAGG 1 cut(s) 1971
Bse8I GATNNNNATC 1 cut(s) 1472
BseBI CCWGG 4 cut(s) 967, 1463, 1785, 2195
BseCI ATCGAT 1 cut(s) 1859
BseDI CCNNGG 4 cut(s) 473, 1306, 1687, 2277
BseGI GGATG 6 cut(s) 415, 761, 975, 1800, 2029, 2190
BseJI GATNNNNATC 1 cut(s) 1472
BseLI CCNNNNNNNGG 5 cut(s) 923, 1268, 1282, 2070, 2081
BseMII CTCAG 8 cut(s) 396, 712, 839, 1064, 1186, 1467, 1787, 1962
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 3 cut(s) 373, 1090, 2409
BseSI GKGCMC 2 cut(s) 235, 1674
BseXI GCAGC 2 cut(s) 615, 2157
BsgI GTGCAG 1 cut(s) 1724
Bsh1236I CGCG 4 cut(s) 105, 1619, 1809, 2068
Bsh1285I CGRYCG 1 cut(s) 2181
BshFI GGCC 6 cut(s) 211, 233, 724, 739, 2074, 2312
BshNI GGYRCC 1 cut(s) 309
BshVI ATCGAT 1 cut(s) 1859
BsiEI CGRYCG 1 cut(s) 2181
BsiHKAI GWGCWC 3 cut(s) 1674, 1733, 1966
BsiHKCI CYCGRG 1 cut(s) 1031
BsiSI CCGG 5 cut(s) 1497, 1502, 2071, 2202, 2309
BslI CCNNNNNNNGG 5 cut(s) 923, 1268, 1282, 2070, 2081
BsmAI GTCTC 2 cut(s) 841, 1986
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 6 cut(s) 211, 233, 724, 739, 2074, 2312
Bso31I GGTCTC 1 cut(s) 1986
BsoBI CYCGRG 1 cut(s) 1031
Bsp120I GGGCCC 1 cut(s) 231
Bsp1286I GDGCHC 4 cut(s) 235, 1674, 1733, 1966
Bsp1407I TGTACA 3 cut(s) 560, 574, 1558
Bsp143I GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
Bsp1720I GCTNAGC 1 cut(s) 188
Bsp19I CCATGG 2 cut(s) 473, 2277
BspACI CCGC 5 cut(s) 368, 786, 907, 1113, 1619
BspANI GGCC 6 cut(s) 211, 233, 724, 739, 2074, 2312
BspCNI CTCAG 8 cut(s) 397, 711, 840, 1065, 1185, 1466, 1788, 1963
BspDI ATCGAT 1 cut(s) 1859
BspFNI CGCG 4 cut(s) 105, 1619, 1809, 2068
BspHI TCATGA 1 cut(s) 2380
BspLI GGNNCC 5 cut(s) 233, 311, 1572, 1639, 1685
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 2 cut(s) 1678, 1691
BspQI GCTCTTC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 1986
BsrBI CCGCTC 1 cut(s) 907
BsrFI RCCGGY 1 cut(s) 2070
BsrGI TGTACA 3 cut(s) 560, 574, 1558
BsrI ACTGG 1 cut(s) 469
BssAI RCCGGY 1 cut(s) 2070
BssECI CCNNGG 4 cut(s) 473, 1306, 1687, 2277
BssMI GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
BssNI GRCGYC 2 cut(s) 2139, 2346
BssT1I CCWWGG 3 cut(s) 473, 1687, 2277
Bst2UI CCWGG 4 cut(s) 967, 1463, 1785, 2195
Bst4CI ACNGT 8 cut(s) 124, 273, 425, 590, 753, 1669, 1771, 1961
Bst6I CTCTTC 2 cut(s) 199, 219
BstACI GRCGYC 2 cut(s) 2139, 2346
BstAUI TGTACA 3 cut(s) 560, 574, 1558
BstBAI YACGTR 4 cut(s) 801, 1193, 1597, 2090
BstC8I GCNNGC 4 cut(s) 827, 1180, 1321, 2072
BstDSI CCRYGG 3 cut(s) 473, 1306, 2277
BstEII GGTNACC 2 cut(s) 1271, 1622
BstF5I GGATG 6 cut(s) 415, 761, 975, 1800, 2029, 2190
BstFNI CGCG 4 cut(s) 105, 1619, 1809, 2068
BstHHI GCGC 1 cut(s) 2070
BstKTI GATC 7 cut(s) 97, 205, 525, 854, 1302, 1594, 1686
BstMAI GTCTC 2 cut(s) 841, 1986
BstMBI GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
BstMCI CGRYCG 1 cut(s) 2181
BstMWI GCNNNNNNNGC 7 cut(s) 40, 61, 1113, 1702, 1806, 2076, 2295
BstNI CCWGG 4 cut(s) 967, 1463, 1785, 2195
BstNSI RCATGY 1 cut(s) 829
BstPAI GACNNNNGTC 1 cut(s) 1504
BstPI GGTNACC 2 cut(s) 1271, 1622
BstSCI CCNGG 4 cut(s) 965, 1461, 1783, 2193
BstSFI CTRYAG 1 cut(s) 1108
BstSLI GKGCMC 2 cut(s) 235, 1674
BstUI CGCG 4 cut(s) 105, 1619, 1809, 2068
BstV1I GCAGC 2 cut(s) 615, 2157
BstV2I GAAGAC 3 cut(s) 1096, 1137, 2391
BstX2I RGATCY 3 cut(s) 851, 1299, 1683
BstXI CCANNNNNNTGG 1 cut(s) 973
BstYI RGATCY 3 cut(s) 851, 1299, 1683
Bsu15I ATCGAT 1 cut(s) 1859
Bsu36I CCTNAGG 1 cut(s) 1971
BsuRI GGCC 6 cut(s) 211, 233, 724, 739, 2074, 2312
BsuTUI ATCGAT 1 cut(s) 1859
BtgI CCRYGG 3 cut(s) 473, 1306, 2277
BtgZI GCGATG 3 cut(s) 1023, 1300, 1790
BtsCI GGATG 6 cut(s) 415, 761, 975, 1800, 2029, 2190
BtsIMutI CAGTG 4 cut(s) 78, 129, 176, 758
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 4 cut(s) 827, 1180, 1321, 2072
CciI TCATGA 1 cut(s) 2380
CfoI GCGC 1 cut(s) 2070
Cfr10I RCCGGY 1 cut(s) 2070
Cfr13I GGNCC 4 cut(s) 209, 231, 232, 1499
ClaI ATCGAT 1 cut(s) 1859
CpoI CGGWCCG 1 cut(s) 1499
CseI GACGC 2 cut(s) 1798, 2147
CsiI ACCWGGT 2 cut(s) 1461, 1783
CspI CGGWCCG 1 cut(s) 1499
DpnI GATC 7 cut(s) 96, 204, 524, 853, 1301, 1593, 1685
DpnII GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
DraI TTTAAA 3 cut(s) 135, 1855, 2394
EaeI YGGCCR 4 cut(s) 722, 737, 2072, 2310
Eam1104I CTCTTC 2 cut(s) 199, 219
EarI CTCTTC 2 cut(s) 199, 219
Ecl136II GAGCTC 1 cut(s) 1731
Eco130I CCWWGG 3 cut(s) 473, 1687, 2277
Eco24I GRGCYC 2 cut(s) 235, 1733
Eco31I GGTCTC 1 cut(s) 1986
Eco32I GATATC 1 cut(s) 1470
Eco47I GGWCC 1 cut(s) 1499
Eco53kI GAGCTC 1 cut(s) 1731
Eco72I CACGTG 2 cut(s) 1193, 1597
Eco81I CCTNAGG 1 cut(s) 1971
Eco88I CYCGRG 1 cut(s) 1031
Eco91I GGTNACC 2 cut(s) 1271, 1622
EcoICRI GAGCTC 1 cut(s) 1731
EcoO109I RGGNCCY 2 cut(s) 231, 232
EcoO65I GGTNACC 2 cut(s) 1271, 1622
EcoRII CCWGG 4 cut(s) 965, 1461, 1783, 2193
EcoRV GATATC 1 cut(s) 1470
EcoT14I CCWWGG 3 cut(s) 473, 1687, 2277
EcoT38I GRGCYC 2 cut(s) 235, 1733
ErhI CCWWGG 3 cut(s) 473, 1687, 2277
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FauI CCCGC 1 cut(s) 1612
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 1773
Fnu4HI GCNGC 3 cut(s) 629, 1114, 2171
FokI GGATG 6 cut(s) 422, 748, 982, 1787, 2016, 2177
FriOI GRGCYC 2 cut(s) 235, 1733
Fsp4HI GCNGC 3 cut(s) 629, 1114, 2171
FspBI CTAG 4 cut(s) 891, 1238, 1920, 2403
GlaI GCGC 1 cut(s) 2069
GluI GCNGC 3 cut(s) 629, 1114, 2171
GsuI CTGGAG 2 cut(s) 70, 2311
HaeIII GGCC 6 cut(s) 211, 233, 724, 739, 2074, 2312
HapII CCGG 5 cut(s) 1497, 1502, 2071, 2202, 2309
HgaI GACGC 2 cut(s) 1798, 2147
HhaI GCGC 1 cut(s) 2070
Hin1I GRCGYC 2 cut(s) 2139, 2346
Hin6I GCGC 1 cut(s) 2068
HinP1I GCGC 1 cut(s) 2068
HindIII AAGCTT 5 cut(s) 129, 569, 1351, 2375, 2396
HinfI GANTC 9 cut(s) 83, 941, 1066, 1096, 1170, 1311, 1505, 1565, 1632
HpaII CCGG 5 cut(s) 1497, 1502, 2071, 2202, 2309
Hpy166II GTNNAC 6 cut(s) 276, 561, 577, 1672, 1714, 1774
Hpy188III TCNNGA 7 cut(s) 49, 436, 840, 980, 1970, 2342, 2381
Hpy8I GTNNAC 6 cut(s) 276, 561, 577, 1672, 1714, 1774
Hpy99I CGWCG 2 cut(s) 2179, 2348
HpyAV CCTTC 3 cut(s) 1060, 1400, 1924
HpyCH4III ACNGT 8 cut(s) 124, 273, 425, 590, 753, 1669, 1771, 1961
HpyCH4IV ACGT 8 cut(s) 255, 800, 1192, 1596, 1721, 2089, 2346, 2388
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 61, 1113, 1702, 1806, 2076, 2295
HpySE526I ACGT 8 cut(s) 255, 800, 1192, 1596, 1721, 2089, 2346, 2388
Hsp92I GRCGYC 2 cut(s) 2139, 2346
HspAI GCGC 1 cut(s) 2068
KroI GCCGGC 1 cut(s) 2070
KroNI GCCGGC 1 cut(s) 2072
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
LguI GCTCTTC 1 cut(s) 199
LmnI GCTCC 1 cut(s) 386
Lsp1109I GCAGC 2 cut(s) 615, 2157
LweI GCATC 6 cut(s) 640, 960, 1012, 1017, 1809, 2199
MabI ACCWGGT 2 cut(s) 1461, 1783
MaeI CTAG 4 cut(s) 891, 1238, 1920, 2403
MaeII ACGT 8 cut(s) 255, 800, 1192, 1596, 1721, 2089, 2346, 2388
MaeIII GTNAC 6 cut(s) 911, 1188, 1271, 1384, 1506, 1622
MalI GATC 7 cut(s) 96, 204, 524, 853, 1301, 1593, 1685
MbiI CCGCTC 1 cut(s) 907
MboI GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
MflI RGATCY 3 cut(s) 851, 1299, 1683
MhlI GDGCHC 4 cut(s) 235, 1674, 1733, 1966
MluI ACGCGT 1 cut(s) 103
MlyI GAGTC 7 cut(s) 77, 935, 1075, 1164, 1514, 1574, 1641
MmeI TCCRAC 3 cut(s) 1548, 1615, 2080
MroNI GCCGGC 1 cut(s) 2070
MroXI GAANNNNTTC 1 cut(s) 2209
MslI CAYNNNNRTG 9 cut(s) 271, 537, 591, 824, 968, 1329, 1447, 1724, 1922
MspA1I CMGCKG 1 cut(s) 2170
MspI CCGG 5 cut(s) 1497, 1502, 2071, 2202, 2309
MspR9I CCNGG 4 cut(s) 967, 1463, 1785, 2195
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 4 cut(s) 967, 1463, 1785, 2195
MvnI CGCG 4 cut(s) 105, 1619, 1809, 2068
MwoI GCNNNNNNNGC 7 cut(s) 40, 61, 1113, 1702, 1806, 2076, 2295
NaeI GCCGGC 1 cut(s) 2072
NcoI CCATGG 2 cut(s) 473, 2277
NdeII GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
NgoMIV GCCGGC 1 cut(s) 2070
NlaIV GGNNCC 5 cut(s) 233, 311, 1572, 1639, 1685
NmuCI GTSAC 5 cut(s) 911, 1188, 1271, 1506, 1622
NspI RCATGY 1 cut(s) 829
OliI CACNNNNGTG 1 cut(s) 271
PaeI GCATGC 1 cut(s) 829
PaeR7I CTCGAG 1 cut(s) 1031
PagI TCATGA 1 cut(s) 2380
PciSI GCTCTTC 1 cut(s) 199
PctI GAATGC 1 cut(s) 748
PdiI GCCGGC 1 cut(s) 2072
PdmI GAANNNNTTC 1 cut(s) 2209
PfeI GAWTC 2 cut(s) 1096, 1311
PflMI CCANNNNNTGG 1 cut(s) 1282
PfoI TCCNGGA 1 cut(s) 2193
PkrI GCNGC 3 cut(s) 630, 1115, 2172
PleI GAGTC 7 cut(s) 77, 935, 1074, 1164, 1513, 1573, 1640
PmaCI CACGTG 2 cut(s) 1193, 1597
PmlI CACGTG 2 cut(s) 1193, 1597
PpsI GAGTC 7 cut(s) 77, 935, 1074, 1164, 1513, 1573, 1640
Ppu21I YACGTR 4 cut(s) 801, 1193, 1597, 2090
PshAI GACNNNNGTC 1 cut(s) 1504
Psp124BI GAGCTC 1 cut(s) 1733
Psp6I CCWGG 4 cut(s) 965, 1461, 1783, 2193
PspCI CACGTG 2 cut(s) 1193, 1597
PspEI GGTNACC 2 cut(s) 1271, 1622
PspGI CCWGG 4 cut(s) 965, 1461, 1783, 2193
PspN4I GGNNCC 5 cut(s) 233, 311, 1572, 1639, 1685
PspOMI GGGCCC 1 cut(s) 231
PspPI GGNCC 4 cut(s) 209, 231, 232, 1499
PspXI VCTCGAGB 1 cut(s) 1031
PsrI GAACNNNNNNTAC 2 cut(s) 794, 826
PsuI RGATCY 3 cut(s) 851, 1299, 1683
PvuII CAGCTG 1 cut(s) 2170
RseI CAYNNNNRTG 9 cut(s) 271, 537, 591, 824, 968, 1329, 1447, 1724, 1922
Rsr2I CGGWCCG 1 cut(s) 1499
RsrII CGGWCCG 1 cut(s) 1499
SacI GAGCTC 1 cut(s) 1733
SapI GCTCTTC 1 cut(s) 199
SatI GCNGC 3 cut(s) 629, 1114, 2171
Sau3AI GATC 7 cut(s) 94, 202, 522, 851, 1299, 1591, 1683
Sau96I GGNCC 4 cut(s) 209, 231, 232, 1499
ScaI AGTACT 1 cut(s) 1236
SchI GAGTC 7 cut(s) 77, 935, 1075, 1164, 1514, 1574, 1641
ScrFI CCNGG 4 cut(s) 967, 1463, 1785, 2195
SduI GDGCHC 4 cut(s) 235, 1674, 1733, 1966
SexAI ACCWGGT 2 cut(s) 1461, 1783
SfaNI GCATC 6 cut(s) 640, 960, 1012, 1017, 1809, 2199
SfcI CTRYAG 1 cut(s) 1108
Sfr274I CTCGAG 1 cut(s) 1031
SinI GGWCC 1 cut(s) 1499
SlaI CTCGAG 1 cut(s) 1031
SmiI ATTTAAAT 1 cut(s) 1855
SmiMI CAYNNNNRTG 9 cut(s) 271, 537, 591, 824, 968, 1329, 1447, 1724, 1922
SmlI CTYRAG 3 cut(s) 59, 1031, 1878
SmoI CTYRAG 3 cut(s) 59, 1031, 1878
SpeI ACTAGT 1 cut(s) 1919
SphI GCATGC 1 cut(s) 829
SsiI CCGC 5 cut(s) 368, 786, 907, 1113, 1619
SspI AATATT 2 cut(s) 1152, 2119
SspMI CTAG 4 cut(s) 891, 1238, 1920, 2403
SstI GAGCTC 1 cut(s) 1733
StyD4I CCNGG 4 cut(s) 965, 1461, 1783, 2193
StyI CCWWGG 3 cut(s) 473, 1687, 2277
SwaI ATTTAAAT 1 cut(s) 1855
TaaI ACNGT 8 cut(s) 124, 273, 425, 590, 753, 1669, 1771, 1961
TaiI ACGT 8 cut(s) 258, 803, 1195, 1599, 1724, 2092, 2349, 2391
TaqI TCGA 9 cut(s) 201, 437, 492, 521, 1010, 1032, 1859, 1952, 2301
TaqII GACCGA 1 cut(s) 2167
TatI WGTACW 4 cut(s) 560, 574, 1234, 1558
TauI GCSGC 1 cut(s) 1116
TfiI GAWTC 2 cut(s) 1096, 1311
TscAI CASTG 4 cut(s) 85, 129, 176, 758
TseFI GTSAC 5 cut(s) 911, 1188, 1271, 1506, 1622
TseI GCWGC 2 cut(s) 628, 2170
Tsp45I GTSAC 5 cut(s) 911, 1188, 1271, 1506, 1622
TspDTI ATGAA 8 cut(s) 344, 704, 750, 1042, 1303, 1839, 2369, 2397
TspGWI ACGGA 1 cut(s) 2112
TspRI CASTG 4 cut(s) 85, 129, 176, 758
Van91I CCANNNNNTGG 1 cut(s) 1282
VneI GTGCAC 1 cut(s) 1670
VpaK11BI GGWCC 1 cut(s) 1499
XapI RAATTY 4 cut(s) 350, 360, 768, 2049
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 5 cut(s) 449, 609, 732, 970, 1334
XhoI CTCGAG 1 cut(s) 1031
XmiI GTMKAC 1 cut(s) 1773
XmnI GAANNNNTTC 1 cut(s) 2209
XspI CTAG 4 cut(s) 891, 1238, 1920, 2403
ZraI GACGTC 1 cut(s) 2347
ZrmI AGTACT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.