pycom12g08360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002049
Physical Location & Seq
Forward (+)
9551602 .. 9555284
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g08360.2

Sequence Viewer

Length: 783 bp
ATGTCGAACATGCTCCGCTTTCGACTTAGAATACACCAGAATATCGTCAATGAAGACAATGACAAATCTATCCAAGTAAGGCTGGAATACTCGGTTCATCAAATCCATAAAGGCGGCTGGTGCATTCGTCAACCCAAATGGCATAACCAGAAACTCGTAATGACCGTAACGAGTCCTGAACGCCGTCTTAGGGACATCATCCCTACTGATCTTCAGCTGATAGCAAGGGATAACGCAAAACAACCAAGAATCACCACCTCGACCCGTCTGGCCGTAGCTACTAGTACCCTGAATCTGCTGGAGCGGTGCAGGTGGTGGCATAAAGGTCTGCTGCGGTTTCTGCTGCTGCTGACCCTGGGGACAATTTGCCGCTCTATGTCCCACCTGTCCACATGTGAAGCAACCGTTGCTACCACGTCTACACTCACCGAAATGTCGGTTGTTACACCTGCGGCAAATAGGGGCTCTGCCTCGCCCACCATCGCCCTGCCTCTGGCCTCTAGCGCCACCAGAAAATCTACCTCCACGACCCTGCCCAGTGGCACTGAAGCCACCACTCGAAGAACTAGAACTAGCTCCACCTCTCTTGAAGTTCCGAGTCTGTCGGGGCCCGAGCGATGCCTGACCTTTGCCTTTGTCATCTTTCTTCTGGTTGCCGTCCTTCTCTTCATCAGTATCGCTCGACATATTCTCGGAGTCCTCGATCCTCAACAGTATCTCATAGAAGTCCCGGTAAGTCTCGCTGTGGACCGTAGACCATTTATACTAATTTCTGGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.04

Weight (kDa)

10.6

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 300, 457
Acc36I ACCTGC 2 cut(s) 300, 457
AccBSI CCGCTC 2 cut(s) 304, 372
AccI GTMKAC 2 cut(s) 419, 754
AciI CCGC 6 cut(s) 16, 114, 304, 334, 370, 452
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 270
AcuI CTGAAG 2 cut(s) 197, 567
AfaI GTAC 1 cut(s) 286
AfiI CCNNNNNNNGG 2 cut(s) 190, 493
AflIII ACRYGT 1 cut(s) 392
AgsI TTSAA 1 cut(s) 590
AhlI ACTAGT 1 cut(s) 281
AjiI CACGTC 1 cut(s) 417
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 3 cut(s) 217, 278, 576
AluI AGCT 3 cut(s) 217, 278, 576
Alw26I GTCTC 1 cut(s) 743
AlwI GGATC 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 3 cut(s) 270, 495, 608
ApaI GGGCCC 1 cut(s) 612
ApeKI GCWGC 3 cut(s) 331, 343, 346
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 3 cut(s) 608, 609, 748
AsuC2I CCSGG 1 cut(s) 731
AsuHPI GGTGA 2 cut(s) 244, 418
AvaI CYCGRG 1 cut(s) 611
AvaII GGWCC 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 612
BanII GRGCYC 2 cut(s) 467, 612
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 318, 330, 333
BccI CCATC 1 cut(s) 488
BceAI ACGGC 3 cut(s) 168, 257, 641
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 731
BcoDI GTCTC 1 cut(s) 743
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 4 cut(s) 282, 501, 567, 573
BfoI RGCGCY 1 cut(s) 507
BfuAI ACCTGC 2 cut(s) 300, 457
BisI GCNGC 6 cut(s) 115, 332, 344, 347, 370, 453
BlsI GCNGC 6 cut(s) 116, 333, 345, 348, 371, 454
Bme1390I CCNGG 2 cut(s) 356, 731
Bme18I GGWCC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 611
BmgBI CACGTC 1 cut(s) 417
BmgT120I GGNCC 3 cut(s) 608, 609, 748
BmiI GGNNCC 2 cut(s) 609, 610
BmrFI CCNGG 2 cut(s) 356, 731
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 1 cut(s) 608
BmuI ACTGGG 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 731
BsaJI CCNNGG 2 cut(s) 354, 355
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 493
Bse1I ACTGG 1 cut(s) 537
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 354, 355
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 190, 493
BseNI ACTGG 1 cut(s) 537
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 3 cut(s) 318, 330, 333
BsgI GTGCAG 1 cut(s) 328
BshFI GGCC 3 cut(s) 272, 497, 610
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 4 cut(s) 206, 364, 373, 713
BslI CCNNNNNNNGG 2 cut(s) 190, 493
BsmAI GTCTC 1 cut(s) 743
BsmFI GGGAC 4 cut(s) 206, 364, 373, 713
BsmI GAATGC 1 cut(s) 123
BsnI GGCC 3 cut(s) 272, 497, 610
BsoBI CYCGRG 1 cut(s) 611
Bsp120I GGGCCC 1 cut(s) 608
Bsp1286I GDGCHC 2 cut(s) 467, 612
Bsp143I GATC 2 cut(s) 208, 703
BspACI CCGC 6 cut(s) 16, 114, 304, 334, 370, 452
BspANI GGCC 3 cut(s) 272, 497, 610
BspLI GGNNCC 2 cut(s) 609, 610
BspMI ACCTGC 2 cut(s) 300, 457
BspPI GGATC 1 cut(s) 698
BsrBI CCGCTC 2 cut(s) 304, 372
BsrI ACTGG 1 cut(s) 537
BssECI CCNNGG 2 cut(s) 354, 355
BssMI GATC 2 cut(s) 208, 703
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 4 cut(s) 166, 406, 714, 752
Bst6I CTCTTC 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 407
BstDEI CTNAG 2 cut(s) 26, 188
BstF5I GGATG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 2 cut(s) 211, 706
BstMAI GTCTC 1 cut(s) 743
BstMBI GATC 2 cut(s) 208, 703
BstMWI GCNNNNNNNGC 4 cut(s) 120, 340, 407, 503
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 2 cut(s) 13, 396
BstSCI CCNGG 2 cut(s) 354, 729
BstSLI GKGCMC 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 318, 330, 333
BstV2I GAAGAC 1 cut(s) 60
BsuRI GGCC 3 cut(s) 272, 497, 610
BtgZI GCGATG 2 cut(s) 466, 631
BtrI CACGTC 1 cut(s) 417
BtsCI GGATG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 543, 544
BveI ACCTGC 2 cut(s) 300, 457
CfoI GCGC 1 cut(s) 506
Cfr13I GGNCC 3 cut(s) 608, 609, 748
Csp6I GTAC 1 cut(s) 285
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 2 cut(s) 10, 393
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 2 cut(s) 26, 188
DpnI GATC 2 cut(s) 210, 705
DpnII GATC 2 cut(s) 208, 703
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 671
Eco24I GRGCYC 2 cut(s) 467, 612
Eco47I GGWCC 1 cut(s) 748
Eco57I CTGAAG 2 cut(s) 197, 567
Eco88I CYCGRG 1 cut(s) 611
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 354
EcoT38I GRGCYC 2 cut(s) 467, 612
FaeI CATG 2 cut(s) 13, 396
FaiI YATR 9 cut(s) 11, 108, 144, 321, 377, 394, 687, 722, 764
FaqI GGGAC 4 cut(s) 206, 364, 373, 713
FatI CATG 2 cut(s) 9, 392
FblI GTMKAC 2 cut(s) 419, 754
Fnu4HI GCNGC 6 cut(s) 115, 332, 344, 347, 370, 453
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 2 cut(s) 467, 612
Fsp4HI GCNGC 6 cut(s) 115, 332, 344, 347, 370, 453
FspBI CTAG 4 cut(s) 282, 501, 567, 573
GlaI GCGC 1 cut(s) 505
GluI GCNGC 6 cut(s) 115, 332, 344, 347, 370, 453
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 3 cut(s) 272, 497, 610
HapII CCGG 1 cut(s) 731
HhaI GCGC 1 cut(s) 506
Hin1II CATG 2 cut(s) 13, 396
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HincII GTYRAC 1 cut(s) 131
HindII GTYRAC 1 cut(s) 131
HinfI GANTC 5 cut(s) 172, 249, 292, 598, 696
HpaII CCGG 1 cut(s) 731
HphI GGTGA 2 cut(s) 244, 418
Hpy166II GTNNAC 5 cut(s) 131, 390, 420, 748, 755
Hpy188I TCNGA 2 cut(s) 597, 695
Hpy188III TCNNGA 2 cut(s) 176, 587
Hpy8I GTNNAC 5 cut(s) 131, 390, 420, 748, 755
HpyAV CCTTC 1 cut(s) 671
HpyCH4III ACNGT 4 cut(s) 166, 406, 714, 752
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 2 cut(s) 123, 309
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 340, 407, 503
HpyF3I CTNAG 2 cut(s) 26, 188
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 2 cut(s) 13, 396
HspAI GCGC 1 cut(s) 504
Kzo9I GATC 2 cut(s) 208, 703
LmnI GCTCC 3 cut(s) 18, 301, 581
Lsp1109I GCAGC 3 cut(s) 318, 330, 333
LweI GCATC 1 cut(s) 608
MaeI CTAG 4 cut(s) 282, 501, 567, 573
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 2 cut(s) 166, 442
MalI GATC 2 cut(s) 210, 705
MbiI CCGCTC 2 cut(s) 304, 372
MboI GATC 2 cut(s) 208, 703
MboII GAAGA 5 cut(s) 65, 203, 573, 638, 658
MhlI GDGCHC 2 cut(s) 467, 612
MluCI AATT 3 cut(s) 363, 768, 778
MlyI GAGTC 3 cut(s) 181, 607, 705
MnlI CCTC 8 cut(s) 268, 481, 501, 508, 532, 592, 710, 717
MseI TTAA 1 cut(s) 781
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 356, 731
Mva1269I GAATGC 1 cut(s) 123
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 120, 340, 407, 503
NciI CCSGG 1 cut(s) 731
NdeII GATC 2 cut(s) 208, 703
NlaIII CATG 2 cut(s) 13, 396
NlaIV GGNNCC 2 cut(s) 609, 610
NspI RCATGY 2 cut(s) 13, 396
PaqCI CACCTGC 2 cut(s) 300, 457
PasI CCCWGGG 1 cut(s) 355
PciI ACATGT 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 699
PctI GAATGC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 249, 292
PkrI GCNGC 6 cut(s) 116, 333, 345, 348, 371, 454
PleI GAGTC 3 cut(s) 180, 606, 704
PpsI GAGTC 3 cut(s) 180, 606, 704
PscI ACATGT 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 609, 610
PspOMI GGGCCC 1 cut(s) 608
PspPI GGNCC 3 cut(s) 608, 609, 748
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 1 cut(s) 781
SatI GCNGC 6 cut(s) 115, 332, 344, 347, 370, 453
Sau3AI GATC 2 cut(s) 208, 703
Sau96I GGNCC 3 cut(s) 608, 609, 748
SchI GAGTC 3 cut(s) 181, 607, 705
ScrFI CCNGG 2 cut(s) 356, 731
SduI GDGCHC 2 cut(s) 467, 612
SfaNI GCATC 1 cut(s) 608
SinI GGWCC 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 431
SpeI ACTAGT 1 cut(s) 281
Sse9I AATT 3 cut(s) 363, 768, 778
SsiI CCGC 6 cut(s) 16, 114, 304, 334, 370, 452
SspMI CTAG 4 cut(s) 282, 501, 567, 573
StyD4I CCNGG 2 cut(s) 354, 729
TaaI ACNGT 4 cut(s) 166, 406, 714, 752
TaiI ACGT 1 cut(s) 419
TaqI TCGA 6 cut(s) 5, 22, 260, 559, 682, 702
TasI AATT 3 cut(s) 363, 768, 778
TauI GCSGC 3 cut(s) 117, 372, 455
TfiI GAWTC 2 cut(s) 249, 292
Tru1I TTAA 1 cut(s) 781
Tru9I TTAA 1 cut(s) 781
TscAI CASTG 2 cut(s) 544, 550
TseI GCWGC 3 cut(s) 331, 343, 346
TspDTI ATGAA 3 cut(s) 66, 86, 658
TspRI CASTG 2 cut(s) 544, 550
VpaK11BI GGWCC 1 cut(s) 748
XceI RCATGY 2 cut(s) 13, 396
XmiI GTMKAC 2 cut(s) 419, 754
XspI CTAG 4 cut(s) 282, 501, 567, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.