pycom01g03780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002049
Physical Location & Seq
Forward (+)
2852364 .. 2853036
673 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03780.3

Sequence Viewer

Length: 489 bp
ATGTCTCACATGATCTGCTTTGGATTTGGAATAAATCAAAATGTTATCGATAAAAACAATGACAAACCTGTCCAAGTACTGCTTAAATACCCGGTTCATCAAATCCATAAAAGTAGCAGGACCATAACGGGTTCTAAATGCCGTCTTAGGGACATCCTCACTCTTAATCTTCAGTTGATAGTACTGATCAAACAAATCGTCGATACGTGGCAACGGATAACGATTCTTAATCGTCACCCGGTTCAGCTGCCGGTAACTGGAGCTCCCCAAGACGAAGTACTAGGTTGTATAAAACCTTTATCTAATAACTCCTTCAAGGATATGGGGTTTGTACTTGGTAATAAGTCGATCACGAACTCTACATCACGATTCGTCGGCAATCCCGGTAAATCATCCGGAAACATGTTGGGAAAATGCCTGACCACTCTAATATCCTCCACACGGCTAGAAGCGTTACCATTTAGTACAACATTTGCCAGATATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.01

Weight (kDa)

9.97

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccIII TCCGGA 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 5 cut(s) 78, 183, 279, 333, 466
AfiI CCNNNNNNNGG 3 cut(s) 148, 257, 441
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 1 cut(s) 316
AluBI AGCT 2 cut(s) 247, 263
AluI AGCT 2 cut(s) 247, 263
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 9
Aor13HI TCCGGA 1 cut(s) 395
ApeKI GCWGC 1 cut(s) 247
ArsI GACNNNNNNTTYG 2 cut(s) 183, 215
AspS9I GGNCC 1 cut(s) 120
AsuC2I CCSGG 3 cut(s) 92, 239, 384
AsuHPI GGTGA 1 cut(s) 227
AvaII GGWCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 234
BceAI ACGGC 2 cut(s) 126, 458
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 3 cut(s) 92, 239, 384
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 281, 446
BisI GCNGC 1 cut(s) 248
BlsI GCNGC 1 cut(s) 249
BmcAI AGTACT 3 cut(s) 78, 183, 279
Bme1390I CCNGG 3 cut(s) 92, 239, 384
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 92, 239, 384
BpmI CTGGAG 1 cut(s) 279
BpuMI CCSGG 3 cut(s) 92, 239, 384
Bsa29I ATCGAT 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 207
BsaWI WCCGGW 1 cut(s) 395
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 257, 441
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 262
BseAI TCCGGA 1 cut(s) 395
BseCI ATCGAT 1 cut(s) 48
BseGI GGATG 2 cut(s) 153, 392
BseLI CCNNNNNNNGG 3 cut(s) 148, 257, 441
BseNI ACTGG 1 cut(s) 262
BseXI GCAGC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 5 cut(s) 92, 239, 251, 384, 396
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 3 cut(s) 148, 257, 441
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 265
Bsp13I TCCGGA 1 cut(s) 395
Bsp143I GATC 3 cut(s) 12, 186, 348
BspDI ATCGAT 1 cut(s) 48
BspEI TCCGGA 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 250
BssMI GATC 3 cut(s) 12, 186, 348
BstBAI YACGTR 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 146
BstF5I GGATG 2 cut(s) 153, 392
BstKTI GATC 3 cut(s) 15, 189, 351
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 12, 186, 348
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 3 cut(s) 90, 237, 382
BstV1I GCAGC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 48
BsuTUI ATCGAT 1 cut(s) 48
BtsCI GGATG 2 cut(s) 153, 392
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 48
Csp6I GTAC 5 cut(s) 77, 182, 278, 332, 465
CviAII CATG 2 cut(s) 10, 403
CviJI RGCY 3 cut(s) 247, 263, 445
CviKI_1 RGCY 3 cut(s) 247, 263, 445
CviQI GTAC 5 cut(s) 77, 182, 278, 332, 465
DdeI CTNAG 1 cut(s) 146
DpnI GATC 3 cut(s) 14, 188, 350
DpnII GATC 3 cut(s) 12, 186, 348
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 265
Eco32I GATATC 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 263
EcoRV GATATC 1 cut(s) 482
EcoT38I GRGCYC 1 cut(s) 265
FaeI CATG 2 cut(s) 13, 406
FaiI YATR 6 cut(s) 11, 108, 125, 290, 323, 404
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 1 cut(s) 164
FatI CATG 2 cut(s) 9, 402
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 248
FokI GGATG 2 cut(s) 140, 379
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 1 cut(s) 248
FspBI CTAG 2 cut(s) 281, 446
GluI GCNGC 1 cut(s) 248
GsuI CTGGAG 1 cut(s) 279
HapII CCGG 5 cut(s) 92, 239, 251, 384, 396
Hin1II CATG 2 cut(s) 13, 406
HinfI GANTC 2 cut(s) 223, 369
HpaII CCGG 5 cut(s) 92, 239, 251, 384, 396
HphI GGTGA 1 cut(s) 227
Hpy188III TCNNGA 3 cut(s) 352, 366, 396
Hpy99I CGWCG 2 cut(s) 203, 377
HpyAV CCTTC 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 206
HpyF3I CTNAG 1 cut(s) 146
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 2 cut(s) 13, 406
Kpn2I TCCGGA 1 cut(s) 395
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 12, 186, 348
LmnI GCTCC 2 cut(s) 260, 268
LpnPI CCDG 9 cut(s) 81, 103, 105, 243, 252, 264, 397, 409, 431
Lsp1109I GCAGC 1 cut(s) 234
MaeI CTAG 2 cut(s) 281, 446
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 3 cut(s) 233, 253, 453
MalI GATC 3 cut(s) 14, 188, 350
MboI GATC 3 cut(s) 12, 186, 348
MboII GAAGA 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 265
MnlI CCTC 2 cut(s) 167, 445
MroI TCCGGA 1 cut(s) 395
MseI TTAA 3 cut(s) 84, 165, 228
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 5 cut(s) 92, 239, 251, 384, 396
MspR9I CCNGG 3 cut(s) 92, 239, 384
NciI CCSGG 3 cut(s) 92, 239, 384
NdeII GATC 3 cut(s) 12, 186, 348
NlaIII CATG 2 cut(s) 13, 406
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 1 cut(s) 406
PciI ACATGT 1 cut(s) 402
PfeI GAWTC 2 cut(s) 223, 369
PkrI GCNGC 1 cut(s) 249
Ppu21I YACGTR 1 cut(s) 207
PscI ACATGT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 120
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 5 cut(s) 78, 183, 279, 333, 466
RsaNI GTAC 5 cut(s) 77, 182, 278, 332, 465
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 3 cut(s) 84, 165, 228
SatI GCNGC 1 cut(s) 248
Sau3AI GATC 3 cut(s) 12, 186, 348
Sau96I GGNCC 1 cut(s) 120
ScaI AGTACT 3 cut(s) 78, 183, 279
ScrFI CCNGG 3 cut(s) 92, 239, 384
SduI GDGCHC 1 cut(s) 265
SetI ASST 6 cut(s) 70, 209, 249, 265, 286, 298
SinI GGWCC 1 cut(s) 120
SspMI CTAG 2 cut(s) 281, 446
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 3 cut(s) 90, 237, 382
TaiI ACGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 48, 201, 347
TatI WGTACW 5 cut(s) 76, 181, 277, 331, 464
TfiI GAWTC 2 cut(s) 223, 369
Tru1I TTAA 3 cut(s) 84, 165, 228
Tru9I TTAA 3 cut(s) 84, 165, 228
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 120
XceI RCATGY 1 cut(s) 406
XspI CTAG 2 cut(s) 281, 446
ZrmI AGTACT 3 cut(s) 78, 183, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.