pycom08g15990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002049
Physical Location & Seq
Forward (+)
15859133 .. 15861631
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15990.7

Sequence Viewer

Length: 1896 bp
ATGGAACCCGAAGCTAGTGAGCTCCCAAAATGCCTCGTGCTAAATGGAGGTGAGCATGTACATATAAGGTATAGAGGATCCTTTCCCCTGGGCAATGTTAGATCTAACAATTTTACAAGCCCTAGTGGAATTGCTCTAAAGGTCTCTTATCTACATATACACCCTTGGGCAACACACATATGCACAACTCATGCACCCTCTAGTCTCCAATCAACGAAAATGACTTACATTAAAAGTATGTATAAATGTTTGTACATATATCCTTATTTTATTGGTAAGAAAAATCTCGTATACCTATGTTATTTAAGTTCTTCTTTGAATCCACATGTCTCAGATTGTCGCAATTTAGCCCCGACAACTCACCTCTCACAACTCAGCACTATCGTTGGGTGTGTAGAACTTTCTATCATTCCGACCCATGATATAGCTGTTCATATAAACACTGTATACGTAGCCCATTTTAGTTCCCAAGGTTGCTGGCCGTCACGTGGAAGTGACGTAACCATGTCTTTACACAAAGTAATAACAACATGTGCAAGCGCAAGTGTGAAAACAAGGTTCAGAGCACGAAGACCTAATGCAGTCCGGGAGGAAACGACACTACAAAAATATGCCGGGGAAATCCTCAGGGAAGCCACCAAAAGTGCTAGCCAACTAGAACCTGGAGGGGCGCAAAACAAAAAGTGTGAGTGGGTAAAAACAAAGCTTTTCAAAAACCATTTCATAAACAAGATTCTAACTCCTCGCCTTAAAACCTGTATACTTCCCAGAAAATATAATATACATATATATATAAGTCATGCACAAAAATATGCCATGTCAAAGCCTCGAAGTAAAATGCAACCGGAGTCACCTAACGTGACCTGTACGGTTGAATCTAGAGCTCAAATCTCCATCTCACTAACTATACCTGCACACGAGTCGAAACCACCTAAAGTGGTCTGTACGACAAGCCTGGGCTGGGCGAATAATCGCAGGTCACCTACGAGTTGGAACCACCTAAAGTATTATGTACGACAGGACTCACGTGAAGAACTGTGCCCTACTCTGGGCGGGAGCACTAACACCGGGGGTGCATGTTATGAGCTTTCTATACATCTTAAATCACTACTGAACATAAACATAAATCATACTTACCTGGCACTTACCTGTGCGTCCACAACACCAATACATATATATATATATATATATGCAACTAATAATGCTAATTTCTGGGAAAAACACGAGTATATAGGTATATACGGAAAACCAAAAGCCCACTCACTGGTATGTAGAAGGGTCGTAACCCCCTTGCCTCGAGTGACCGCGCTCGTCCTCAGGATAGGTCTCATCTATATGCGAAACAACTATAAAAACGTTAATTTTAAAACACATAACCAACCTTATGAAATAACTTCTCATACAATGCTTAAATGGGGTAAAAAAATTTCACCGTCGCACGCGCCCCTACGCGCCGGTCACGCGCAGGCACGTGTCTGGCACACTGACGGCGTCAGGAATATTCCGTTAACTTTAACGGATTCCGTTAACGCCGTTAGGAATATTCCGTCCAAACTAACGGAATATTCCGTCGTCCTCTCCGGCGAGGCTCCGGCGCCGGAAAATCGTAAAAACTTTAAACCTTCATATCTTCTTCGTTTTTCAACCAAATTTCACAAAATTGGCACCAAAATGAAGCTTACAACGAGAGGAACAAATCCATACCACTTTCAAGTGCTAAAACACACTGAATCTCGCCGGGAAAACACCACCAACTCCGGCCAACCTCGAAACTCACGATCGATCCGACGTCCAAAACCTTCAAACGAACCACCCCGACCTGAGTTCGACGTGAAAACGAAGGTATCCTTACCTGAAATGGGTATGGTTGAACTTCTATGGACCTCACGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

632

Amino Acids

71.4

Weight (kDa)

9.89

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1792
Acc36I ACCTGC 2 cut(s) 919, 966
AccB1I GGYRCC 2 cut(s) 1594, 1664
AccB7I CCANNNNNTGG 1 cut(s) 1264
AccI GTMKAC 3 cut(s) 291, 447, 760
AccII CGCG 4 cut(s) 1307, 1442, 1452, 1463
AciI CCGC 2 cut(s) 1053, 1305
AclI AACGTT 1 cut(s) 1356
AclWI GGATC 3 cut(s) 72, 85, 1777
AcoI YGGCCR 2 cut(s) 479, 1759
AcsI RAATTY 2 cut(s) 1425, 1649
AcvI CACGTG 3 cut(s) 488, 1028, 1472
AcyI GRCGYC 3 cut(s) 1491, 1595, 1789
AfaI GTAC 5 cut(s) 60, 254, 868, 946, 1014
AfiI CCNNNNNNNGG 6 cut(s) 488, 961, 1048, 1049, 1264, 1859
AflIII ACRYGT 3 cut(s) 325, 530, 1471
AgsI TTSAA 7 cut(s) 319, 712, 875, 1644, 1712, 1803, 1871
AjiI CACGTC 1 cut(s) 1831
AjnI CCWGG 4 cut(s) 87, 661, 954, 1137
AluBI AGCT 7 cut(s) 14, 22, 428, 706, 884, 1087, 1678
AluI AGCT 7 cut(s) 14, 22, 428, 706, 884, 1087, 1678
Alw21I GWGCWC 4 cut(s) 24, 568, 886, 1061
Alw26I GTCTC 4 cut(s) 148, 209, 334, 1331
AlwI GGATC 3 cut(s) 72, 85, 1777
Ama87I CYCGRG 1 cut(s) 1296
AoxI GGCC 2 cut(s) 479, 1759
ApoI RAATTY 2 cut(s) 1425, 1649
AspLEI GCGC 7 cut(s) 542, 673, 1309, 1444, 1454, 1465, 1597
AspS9I GGNCC 1 cut(s) 1881
AsuC2I CCSGG 4 cut(s) 587, 616, 1069, 1739
AsuHPI GGTGA 5 cut(s) 62, 353, 843, 972, 1422
AsuNHI GCTAGC 1 cut(s) 647
AvaI CYCGRG 1 cut(s) 1296
AvaII GGWCC 1 cut(s) 1881
AxyI CCTNAGG 2 cut(s) 626, 1316
BaeGI GKGCMC 1 cut(s) 1043
BaeI ACNNNNGTAYC 2 cut(s) 1827, 1860
BamHI GGATCC 1 cut(s) 77
BanI GGYRCC 2 cut(s) 1594, 1664
BanII GRGCYC 2 cut(s) 24, 886
BauI CACGAG 4 cut(s) 35, 917, 1223, 1887
BbrPI CACGTG 3 cut(s) 488, 1028, 1472
BbsI GAAGAC 1 cut(s) 577
Bbv12I GWGCWC 4 cut(s) 24, 568, 886, 1061
BccI CCATC 1 cut(s) 902
BceAI ACGGC 3 cut(s) 466, 1504, 1517
BcgI CGANNNNNNTGC 2 cut(s) 903, 937
BciT130I CCWGG 4 cut(s) 89, 663, 956, 1139
BciVI GTATCC 1 cut(s) 1855
BcnI CCSGG 4 cut(s) 587, 616, 1069, 1739
BcoDI GTCTC 4 cut(s) 148, 209, 334, 1331
BfaI CTAG 6 cut(s) 15, 123, 201, 648, 656, 879
BfoI RGCGCY 1 cut(s) 1598
BfuAI ACCTGC 2 cut(s) 919, 966
BfuI GTATCC 1 cut(s) 1855
BglII AGATCT 1 cut(s) 101
Bme1390I CCNGG 8 cut(s) 89, 587, 616, 663, 956, 1069, 1139, 1739
Bme18I GGWCC 1 cut(s) 1881
BmeT110I CYCGRG 1 cut(s) 1296
BmgBI CACGTC 1 cut(s) 1831
BmgT120I GGNCC 1 cut(s) 1881
BmiI GGNNCC 6 cut(s) 6, 79, 995, 1590, 1596, 1666
BmrFI CCNGG 8 cut(s) 89, 587, 616, 663, 956, 1069, 1139, 1739
BmtI GCTAGC 1 cut(s) 651
BpiI GAAGAC 1 cut(s) 577
BpmI CTGGAG 1 cut(s) 684
BpuMI CCSGG 4 cut(s) 587, 616, 1069, 1739
Bsa29I ATCGAT 1 cut(s) 1781
BsaAI YACGTR 4 cut(s) 451, 488, 1028, 1472
BsaHI GRCGYC 3 cut(s) 1491, 1595, 1789
BsaI GGTCTC 2 cut(s) 148, 1331
BsaJI CCNNGG 7 cut(s) 87, 88, 164, 469, 615, 955, 1068
BsaWI WCCGGW 1 cut(s) 844
Bsc4I CCNNNNNNNGG 6 cut(s) 488, 961, 1048, 1049, 1264, 1859
Bse118I RCCGGY 1 cut(s) 1454
Bse1I ACTGG 1 cut(s) 1269
Bse21I CCTNAGG 2 cut(s) 626, 1316
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 4 cut(s) 89, 663, 956, 1139
BseCI ATCGAT 1 cut(s) 1781
BseDI CCNNGG 7 cut(s) 87, 88, 164, 469, 615, 955, 1068
BseLI CCNNNNNNNGG 6 cut(s) 488, 961, 1048, 1049, 1264, 1859
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 5 cut(s) 345, 388, 640, 1330, 1812
BseNI ACTGG 1 cut(s) 1269
BseRI GAGGAG 1 cut(s) 732
BseSI GKGCMC 1 cut(s) 1043
BseYI CCCAGC 1 cut(s) 960
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 4 cut(s) 1307, 1442, 1452, 1463
Bsh1285I CGRYCG 1 cut(s) 1781
BshFI GGCC 2 cut(s) 481, 1761
BshNI GGYRCC 2 cut(s) 1594, 1664
BshVI ATCGAT 1 cut(s) 1781
BsiEI CGRYCG 1 cut(s) 1781
BsiHKAI GWGCWC 4 cut(s) 24, 568, 886, 1061
BsiHKCI CYCGRG 1 cut(s) 1296
BslI CCNNNNNNNGG 6 cut(s) 488, 961, 1048, 1049, 1264, 1859
BsmAI GTCTC 4 cut(s) 148, 209, 334, 1331
BsnI GGCC 2 cut(s) 481, 1761
Bso31I GGTCTC 2 cut(s) 148, 1331
BsoBI CYCGRG 1 cut(s) 1296
Bsp1286I GDGCHC 5 cut(s) 24, 568, 886, 1043, 1061
Bsp1407I TGTACA 2 cut(s) 58, 252
Bsp143I GATC 4 cut(s) 77, 101, 1778, 1782
BspACI CCGC 2 cut(s) 1053, 1305
BspANI GGCC 2 cut(s) 481, 1761
BspCNI CTCAG 5 cut(s) 344, 387, 639, 1329, 1813
BspDI ATCGAT 1 cut(s) 1781
BspFNI CGCG 4 cut(s) 1307, 1442, 1452, 1463
BspLI GGNNCC 6 cut(s) 6, 79, 995, 1590, 1596, 1666
BspMI ACCTGC 2 cut(s) 919, 966
BspOI GCTAGC 1 cut(s) 651
BspPI GGATC 3 cut(s) 72, 85, 1777
BspT107I GGYRCC 2 cut(s) 1594, 1664
BspTNI GGTCTC 2 cut(s) 148, 1331
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 1454
BsrGI TGTACA 2 cut(s) 58, 252
BsrI ACTGG 1 cut(s) 1269
BssAI RCCGGY 1 cut(s) 1454
BssECI CCNNGG 7 cut(s) 87, 88, 164, 469, 615, 955, 1068
BssMI GATC 4 cut(s) 77, 101, 1778, 1782
BssNAI GTATAC 3 cut(s) 292, 448, 761
BssNI GRCGYC 3 cut(s) 1491, 1595, 1789
BssSI CACGAG 4 cut(s) 35, 917, 1223, 1887
BssT1I CCWWGG 2 cut(s) 164, 469
Bst1107I GTATAC 3 cut(s) 292, 448, 761
Bst2BI CACGAG 4 cut(s) 35, 917, 1223, 1887
Bst2UI CCWGG 4 cut(s) 89, 663, 956, 1139
Bst4CI ACNGT 4 cut(s) 445, 871, 1038, 1434
BstACI GRCGYC 3 cut(s) 1491, 1595, 1789
BstAUI TGTACA 2 cut(s) 58, 252
BstBAI YACGTR 4 cut(s) 451, 488, 1028, 1472
BstC8I GCNNGC 5 cut(s) 479, 538, 649, 1440, 1467
BstDEI CTNAG 5 cut(s) 331, 374, 626, 1316, 1821
BstEII GGTNACC 1 cut(s) 978
BstFNI CGCG 4 cut(s) 1307, 1442, 1452, 1463
BstH2I RGCGCY 1 cut(s) 1598
BstHHI GCGC 7 cut(s) 542, 673, 1309, 1444, 1454, 1465, 1597
BstKTI GATC 4 cut(s) 80, 104, 1781, 1785
BstMAI GTCTC 4 cut(s) 148, 209, 334, 1331
BstMBI GATC 4 cut(s) 77, 101, 1778, 1782
BstMCI CGRYCG 1 cut(s) 1781
BstMWI GCNNNNNNNGC 1 cut(s) 1460
BstNI CCWGG 4 cut(s) 89, 663, 956, 1139
BstNSI RCATGY 4 cut(s) 59, 329, 534, 1080
BstPI GGTNACC 1 cut(s) 978
BstSCI CCNGG 8 cut(s) 87, 585, 614, 661, 954, 1067, 1137, 1737
BstSLI GKGCMC 1 cut(s) 1043
BstSNI TACGTA 1 cut(s) 451
BstUI CGCG 4 cut(s) 1307, 1442, 1452, 1463
BstV2I GAAGAC 1 cut(s) 577
BstX2I RGATCY 2 cut(s) 77, 101
BstYI RGATCY 2 cut(s) 77, 101
BstZ17I GTATAC 3 cut(s) 292, 448, 761
Bsu15I ATCGAT 1 cut(s) 1781
Bsu36I CCTNAGG 2 cut(s) 626, 1316
BsuI GTATCC 1 cut(s) 1855
BsuRI GGCC 2 cut(s) 481, 1761
BsuTUI ATCGAT 1 cut(s) 1781
BtrI CACGTC 1 cut(s) 1831
BtsIMutI CAGTG 4 cut(s) 441, 1262, 1482, 1725
BveI ACCTGC 2 cut(s) 919, 966
Cac8I GCNNGC 5 cut(s) 479, 538, 649, 1440, 1467
CfoI GCGC 7 cut(s) 542, 673, 1309, 1444, 1454, 1465, 1597
Cfr10I RCCGGY 1 cut(s) 1454
Cfr13I GGNCC 1 cut(s) 1881
ClaI ATCGAT 1 cut(s) 1781
CseI GACGC 2 cut(s) 1143, 1480
Csp6I GTAC 5 cut(s) 59, 253, 867, 945, 1013
CviQI GTAC 5 cut(s) 59, 253, 867, 945, 1013
DdeI CTNAG 5 cut(s) 331, 374, 626, 1316, 1821
DinI GGCGCC 1 cut(s) 1596
DpnI GATC 4 cut(s) 79, 103, 1780, 1784
DpnII GATC 4 cut(s) 77, 101, 1778, 1782
DraI TTTAAA 2 cut(s) 1366, 1618
EaeI YGGCCR 2 cut(s) 479, 1759
Ecl136II GAGCTC 2 cut(s) 22, 884
Eco105I TACGTA 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 164, 469
Eco24I GRGCYC 2 cut(s) 24, 886
Eco31I GGTCTC 2 cut(s) 148, 1331
Eco47I GGWCC 1 cut(s) 1881
Eco53kI GAGCTC 2 cut(s) 22, 884
Eco72I CACGTG 3 cut(s) 488, 1028, 1472
Eco81I CCTNAGG 2 cut(s) 626, 1316
Eco88I CYCGRG 1 cut(s) 1296
Eco91I GGTNACC 1 cut(s) 978
EcoICRI GAGCTC 2 cut(s) 22, 884
EcoO65I GGTNACC 1 cut(s) 978
EcoRII CCWGG 4 cut(s) 87, 661, 954, 1137
EcoT14I CCWWGG 2 cut(s) 164, 469
EcoT38I GRGCYC 2 cut(s) 24, 886
EgeI GGCGCC 1 cut(s) 1596
EheI GGCGCC 1 cut(s) 1596
ErhI CCWWGG 2 cut(s) 164, 469
FalI AAGNNNNNCTT 4 cut(s) 298, 330, 1832, 1864
FauI CCCGC 1 cut(s) 1046
FauNDI CATATG 1 cut(s) 179
FblI GTMKAC 3 cut(s) 291, 447, 760
FriOI GRGCYC 2 cut(s) 24, 886
FspBI CTAG 6 cut(s) 15, 123, 201, 648, 656, 879
GlaI GCGC 7 cut(s) 541, 672, 1308, 1443, 1453, 1464, 1596
GsaI CCCAGC 1 cut(s) 964
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 1598
HaeIII GGCC 2 cut(s) 481, 1761
HgaI GACGC 2 cut(s) 1143, 1480
HhaI GCGC 7 cut(s) 542, 673, 1309, 1444, 1454, 1465, 1597
Hin1I GRCGYC 3 cut(s) 1491, 1595, 1789
Hin6I GCGC 7 cut(s) 540, 671, 1307, 1442, 1452, 1463, 1595
HinP1I GCGC 7 cut(s) 540, 671, 1307, 1442, 1452, 1463, 1595
HincII GTYRAC 2 cut(s) 1509, 1528
HindII GTYRAC 2 cut(s) 1509, 1528
HindIII AAGCTT 2 cut(s) 704, 1676
HinfI GANTC 8 cut(s) 319, 733, 848, 875, 920, 1022, 1520, 1730
HpaI GTTAAC 2 cut(s) 1509, 1528
HphI GGTGA 5 cut(s) 62, 353, 843, 972, 1422
Hpy166II GTNNAC 6 cut(s) 292, 448, 761, 1158, 1509, 1528
Hpy188I TCNGA 4 cut(s) 334, 414, 563, 1787
Hpy188III TCNNGA 5 cut(s) 879, 1318, 1495, 1776, 1887
Hpy8I GTNNAC 6 cut(s) 292, 448, 761, 1158, 1509, 1528
Hpy99I CGWCG 4 cut(s) 1438, 1574, 1791, 1832
HpyAV CCTTC 4 cut(s) 1269, 1632, 1809, 1834
HpyCH4III ACNGT 4 cut(s) 445, 871, 1038, 1434
HpyCH4IV ACGT 9 cut(s) 450, 487, 498, 858, 1027, 1356, 1471, 1789, 1830
HpyCH4V TGCA 9 cut(s) 183, 194, 536, 581, 803, 841, 914, 1076, 1193
HpyF10VI GCNNNNNNNGC 1 cut(s) 1460
HpyF3I CTNAG 5 cut(s) 331, 374, 626, 1316, 1821
HpySE526I ACGT 9 cut(s) 450, 487, 498, 858, 1027, 1356, 1471, 1789, 1830
Hsp92I GRCGYC 3 cut(s) 1491, 1595, 1789
HspAI GCGC 7 cut(s) 540, 671, 1307, 1442, 1452, 1463, 1595
KasI GGCGCC 1 cut(s) 1594
KspAI GTTAAC 2 cut(s) 1509, 1528
Kzo9I GATC 4 cut(s) 77, 101, 1778, 1782
LmnI GCTCC 3 cut(s) 27, 1056, 1594
MaeI CTAG 6 cut(s) 15, 123, 201, 648, 656, 879
MaeII ACGT 9 cut(s) 450, 487, 498, 858, 1027, 1356, 1471, 1789, 1830
MaeIII GTNAC 9 cut(s) 483, 494, 499, 849, 859, 978, 1282, 1300, 1457
MalI GATC 4 cut(s) 79, 103, 1780, 1784
MboI GATC 4 cut(s) 77, 101, 1778, 1782
MboII GAAGA 5 cut(s) 303, 582, 1043, 1622, 1625
MflI RGATCY 2 cut(s) 77, 101
MhlI GDGCHC 5 cut(s) 24, 568, 886, 1043, 1061
MluCI AATT 8 cut(s) 109, 129, 343, 1207, 1360, 1425, 1649, 1659
Mly113I GGCGCC 1 cut(s) 1595
MlyI GAGTC 3 cut(s) 857, 929, 1016
MmeI TCCRAC 3 cut(s) 437, 971, 1810
MslI CAYNNNNRTG 5 cut(s) 178, 1267, 1334, 1670, 1891
MspR9I CCNGG 8 cut(s) 89, 587, 616, 663, 956, 1069, 1139, 1739
MvaI CCWGG 4 cut(s) 89, 663, 956, 1139
MvnI CGCG 4 cut(s) 1307, 1442, 1452, 1463
MwoI GCNNNNNNNGC 1 cut(s) 1460
NarI GGCGCC 1 cut(s) 1595
NciI CCSGG 4 cut(s) 587, 616, 1069, 1739
NdeI CATATG 1 cut(s) 179
NdeII GATC 4 cut(s) 77, 101, 1778, 1782
NheI GCTAGC 1 cut(s) 647
NlaIV GGNNCC 6 cut(s) 6, 79, 995, 1590, 1596, 1666
NmuCI GTSAC 7 cut(s) 483, 494, 849, 859, 978, 1300, 1457
NspI RCATGY 4 cut(s) 59, 329, 534, 1080
PaeR7I CTCGAG 1 cut(s) 1296
PasI CCCWGGG 1 cut(s) 88
PciI ACATGT 2 cut(s) 325, 530
PcsI WCGNNNNNNNCGW 2 cut(s) 1774, 1783
PfeI GAWTC 5 cut(s) 319, 733, 875, 1520, 1730
PflFI GACNNNGTC 1 cut(s) 1490
PflMI CCANNNNNTGG 1 cut(s) 1264
PfoI TCCNGGA 1 cut(s) 585
Ple19I CGATCG 1 cut(s) 1781
PleI GAGTC 3 cut(s) 856, 928, 1016
PluTI GGCGCC 1 cut(s) 1598
PmaCI CACGTG 3 cut(s) 488, 1028, 1472
PmlI CACGTG 3 cut(s) 488, 1028, 1472
PpsI GAGTC 3 cut(s) 856, 928, 1016
Ppu21I YACGTR 4 cut(s) 451, 488, 1028, 1472
PscI ACATGT 2 cut(s) 325, 530
Psp124BI GAGCTC 2 cut(s) 24, 886
Psp1406I AACGTT 1 cut(s) 1356
Psp6I CCWGG 4 cut(s) 87, 661, 954, 1137
PspCI CACGTG 3 cut(s) 488, 1028, 1472
PspEI GGTNACC 1 cut(s) 978
PspFI CCCAGC 1 cut(s) 960
PspGI CCWGG 4 cut(s) 87, 661, 954, 1137
PspN4I GGNNCC 6 cut(s) 6, 79, 995, 1590, 1596, 1666
PspPI GGNCC 1 cut(s) 1881
PspXI VCTCGAGB 1 cut(s) 1296
PsuI RGATCY 2 cut(s) 77, 101
PsyI GACNNNGTC 1 cut(s) 1490
PvuI CGATCG 1 cut(s) 1781
RsaI GTAC 5 cut(s) 60, 254, 868, 946, 1014
RsaNI GTAC 5 cut(s) 59, 253, 867, 945, 1013
RseI CAYNNNNRTG 5 cut(s) 178, 1267, 1334, 1670, 1891
SacI GAGCTC 2 cut(s) 24, 886
Sau3AI GATC 4 cut(s) 77, 101, 1778, 1782
Sau96I GGNCC 1 cut(s) 1881
SchI GAGTC 3 cut(s) 857, 929, 1016
ScrFI CCNGG 8 cut(s) 89, 587, 616, 663, 956, 1069, 1139, 1739
SduI GDGCHC 5 cut(s) 24, 568, 886, 1043, 1061
SfoI GGCGCC 1 cut(s) 1596
Sfr274I CTCGAG 1 cut(s) 1296
SinI GGWCC 1 cut(s) 1881
SlaI CTCGAG 1 cut(s) 1296
SmiMI CAYNNNNRTG 5 cut(s) 178, 1267, 1334, 1670, 1891
SmlI CTYRAG 1 cut(s) 1296
SmoI CTYRAG 1 cut(s) 1296
SnaBI TACGTA 1 cut(s) 451
Sse9I AATT 8 cut(s) 109, 129, 343, 1207, 1360, 1425, 1649, 1659
SsiI CCGC 2 cut(s) 1053, 1305
SspDI GGCGCC 1 cut(s) 1594
SspI AATATT 3 cut(s) 1501, 1543, 1565
SspMI CTAG 6 cut(s) 15, 123, 201, 648, 656, 879
SstI GAGCTC 2 cut(s) 24, 886
StyD4I CCNGG 8 cut(s) 87, 585, 614, 661, 954, 1067, 1137, 1737
StyI CCWWGG 2 cut(s) 164, 469
TaaI ACNGT 4 cut(s) 445, 871, 1038, 1434
TaiI ACGT 9 cut(s) 453, 490, 501, 861, 1030, 1359, 1474, 1792, 1833
TaqI TCGA 6 cut(s) 829, 923, 1297, 1768, 1781, 1827
TasI AATT 8 cut(s) 109, 129, 343, 1207, 1360, 1425, 1649, 1659
TatI WGTACW 2 cut(s) 58, 252
TfiI GAWTC 5 cut(s) 319, 733, 875, 1520, 1730
TscAI CASTG 4 cut(s) 448, 1269, 1489, 1732
TseFI GTSAC 7 cut(s) 483, 494, 849, 859, 978, 1300, 1457
Tsp45I GTSAC 7 cut(s) 483, 494, 849, 859, 978, 1300, 1457
TspDTI ATGAA 5 cut(s) 422, 712, 1401, 1614, 1688
TspGWI ACGGA 7 cut(s) 1257, 1494, 1513, 1532, 1536, 1558, 1574
TspRI CASTG 4 cut(s) 448, 1269, 1489, 1732
Tth111I GACNNNGTC 1 cut(s) 1490
Van91I CCANNNNNTGG 1 cut(s) 1264
VpaK11BI GGWCC 1 cut(s) 1881
XapI RAATTY 2 cut(s) 1425, 1649
XbaI TCTAGA 1 cut(s) 878
XceI RCATGY 4 cut(s) 59, 329, 534, 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XhoI CTCGAG 1 cut(s) 1296
XmiI GTMKAC 3 cut(s) 291, 447, 760
XspI CTAG 6 cut(s) 15, 123, 201, 648, 656, 879
ZraI GACGTC 1 cut(s) 1790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.