pycom01g03240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002049
Physical Location & Seq
Forward (+)
2543321 .. 2546528
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03240.17

Sequence Viewer

Length: 2457 bp
ATGGGAATGGGGACTCGGGACAGTGTGCAGGAAACAGGAGGAAAGGTGGTAGAGTGTGGGAAAGTGTGTGTGGTCTACAACCTACCCACCTTAGGGGTAAAATTGTCTTTTCACGCGTACGTTTTTAAAATTCTCAGAACGGGCTGTCACAACCTACCCACCTTAATAGAATTTCGTCCCGAAAATCAAAACAACCAAACAACAACCCCTAGTGATCAAAGAACAACCGAGGATACAAATCCCTCATCCGATCTTCTGTTTCCCACGTATCTTGTTCCTCAAAACTTTTTCCTTCCAATCTATGATCATCACCGGTTCCTCGTCATAAGTCAAATCTGGATTAATTTCCAAGGGTTAAGGAGGTATCACATGAGACGGATTCGCAACATAATGTCGAAGCATAGACACGTGGAAAACGTTATGTACTCTAGCCAACTTCGGAGGTATGGTCCAATGTACCTGGGACTCAATTTACCCTTCTTTCCAAACCGCACGACACCTCTCCATGGTGAAAGTTTCAGAAATACCCAATCGCCAACCTCATACGCTCTGTCCGTAGCATGCCGATCTGCTAAACTCTTCTGCCTGTCTTGGTCTCCTCAACAATCTCCGAACCCACTAAGACTCTTTCTCCAACCTCTGACCAACACAACGGTGTGCGACAAGATCTACCATACAATGCCTCAAATGGCGCCATGCCGATACTCGAATGAAAGCTGTTGTTGTAGGCAAATTCCATCAAATCCAACCGCTGGTGCCAAGCATCACCAAACTGTAGCACCGAAGCTCGCAACATACCTTCCAAAGTCTGAATGGTTCTCTCTGACTGTCTATCCGTCTGAGGATGATACGTCGTACTATAAAGTAGCCTCCATGGATCGATCGAAACGATCCGAGACAATACTCACAGGGACACCATGGTACTTCACAATCTTCGAGATAAACAACTCCGCTAATCGGCCCAAAGAATACTTCTCTCTCACTGGAATAAAATGTGCCGACTTAGTGAGCCGATCAACGATCACCCAAATGCCGTCAAAACCATTATGTGTACTTCCACTCTGGAACGGGAAGCGGCTGCAACAATCCAAACGACTTCTTCCTTTCCGCTTTAACCTGCTGACAAACAGCACACCTACTCACATACTCAGCTATCTCCCTCTTCATACCCGGCCAATAATAAAATGGTCGAATGGTATGATACATTTTAGTAGCTCCTGGATGCATGGCGTAAGCCGAAATATGCACGAACATTCTACCCTCTTGCATCAACATGCCATCCGAATCACGAACTCTGAGATCTCTCCTCCTTCCTCGATCTCGACTTCAAGCACCCGATCAACTAAAATTGTCCGCCAAAAGAAGAATACGACTTGCATACAACACATTAATACGGCCCTGAGACTTCCTGCTAAGTGCATCAGCAACTACATTTGCACGACCAGGGTGATAATCAATCGTGCAATCGTAATCGCTGAGCAACTCCATTCACCTCCGTTGACGAAGATTAAGTTCCTTCTGAGTAAAAAGATACTGAAGACTCTTATGATCTGTAAAGATCCTACATTTCTCCCCATAGAGGTAGTGTCTCCACAACTTCAACGCAAAGATAATAGCTGTTTCAACTATCGTGAAACCATTCAAGGAAGCATCACTATAAACCTCGAAATCACCACTATCGTCCGGGAGTGCCAAAACAGGTGCATGAGTGAGACAATGCTTCAATTGCTGGAAACTCTGCTCACACTTATCATCCCACTCAAATTTAACATCTTTCCTTACCCTGTTAAACCAAGGAAACTCCTCACCTCTGTGACGGTTCGCGGTTCTGAAATGATGTGTCCCAAAAACGCAACTTGGTCTAACCAAAACTGGCACTTGCTAAACTTAGCATACAATTGGTGTTCCCTCAACCTTTTCAACACCAAAGTAAGATGTCGAACATGCTCCGCTTTCGACTTCGAATACACCAAAATGTCGTCGATGAAAACAATAACAAACCTATCCAAAGATGGCTGGAACACCCTGTTCATTAAATCCATAAAAGCAGCTGGTGTGTTCGTCAACCCAAATGGCATAACCAGAAACTCCCTTACTAATCTTCAGCTTATAATATCCAGACCTCAAGTCAATCTTGGAGAATACACAAGCACCTCGAAGCTGATCAAACAAATCATCGATACGTGGCAACGGATAACGGTTTTTAATCGTCACCCGATTCAATTGCCTGTAATCAATACACAGCCTCAAAGGTTCGTCTTTCTTTCTCACAAACAATACTGGACCACTAATTCCTTCAAGGAAATAGGATTAGTACCTGGAAGCAGCTCAATAGTGAACTCCACATCTCGATCTGGTGGTAAACCAGGTAAATCCTCGGGGAAAACATCGGGAAAGTGTCTGACTACCCTCACATCCTCAACTCTACTAGGAACGGCCTCATCCAACACCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

819

Amino Acids

93.16

Weight (kDa)

9.89

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2111
Acc36I ACCTGC 1 cut(s) 1125
AccB1I GGYRCC 2 cut(s) 691, 755
AccB7I CCANNNNNTGG 1 cut(s) 752
AccI GTMKAC 1 cut(s) 75
AccII CGCG 2 cut(s) 116, 1824
AciI CCGC 8 cut(s) 490, 750, 951, 1075, 1108, 1354, 1824, 1950
AclI AACGTT 1 cut(s) 417
AclWI GGATC 3 cut(s) 885, 885, 1553
AcoI YGGCCR 1 cut(s) 1172
AcsI RAATTY 4 cut(s) 129, 170, 732, 1763
AcuI CTGAAG 2 cut(s) 1556, 2087
AcvI CACGTG 1 cut(s) 409
AcyI GRCGYC 1 cut(s) 692
AfaI GTAC 7 cut(s) 119, 425, 458, 857, 923, 1053, 2316
AfiI CCNNNNNNNGG 6 cut(s) 92, 93, 506, 752, 957, 1579
AflIII ACRYGT 2 cut(s) 114, 406
AgeI ACCGGT 1 cut(s) 312
AgsI TTSAA 8 cut(s) 1329, 1601, 1624, 1643, 1724, 1921, 2222, 2299
AhdI GACNNNNNGTC 1 cut(s) 2126
AjnI CCWGG 5 cut(s) 459, 1217, 1442, 2317, 2365
AjuI GAANNNNNNNTTGG 2 cut(s) 956, 988
AleI CACNNNNGTG 2 cut(s) 653, 1811
AluBI AGCT 9 cut(s) 717, 787, 1152, 1215, 1617, 2051, 2107, 2161, 2328
AluI AGCT 9 cut(s) 717, 787, 1152, 1215, 1617, 2051, 2107, 2161, 2328
Alw26I GTCTC 6 cut(s) 367, 600, 890, 1396, 1593, 1706
AlwI GGATC 3 cut(s) 885, 885, 1553
Ama87I CYCGRG 2 cut(s) 15, 2377
AoxI GGCC 4 cut(s) 959, 1172, 1395, 2436
ApeKI GCWGC 3 cut(s) 1078, 2048, 2325
ApoI RAATTY 4 cut(s) 129, 170, 732, 1763
AseI ATTAAT 2 cut(s) 342, 1389
AsiGI ACCGGT 1 cut(s) 312
Asp700I GAANNNNTTC 1 cut(s) 1638
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 4 cut(s) 449, 960, 1396, 2283
AsuC2I CCSGG 2 cut(s) 1171, 1685
AsuHPI GGTGA 9 cut(s) 302, 521, 758, 1015, 1459, 1482, 1663, 1798, 2204
AsuII TTCGAA 1 cut(s) 1962
AvaI CYCGRG 2 cut(s) 15, 2377
AvaII GGWCC 2 cut(s) 449, 2283
AxyI CCTNAGG 1 cut(s) 91
BanI GGYRCC 2 cut(s) 691, 755
BbrPI CACGTG 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 1544
BbvI GCAGC 3 cut(s) 1065, 2060, 2337
BccI CCATC 3 cut(s) 745, 1286, 2006
BceAI ACGGC 3 cut(s) 1018, 1410, 2451
BcgI CGANNNNNNTGC 2 cut(s) 2206, 2240
BciT130I CCWGG 5 cut(s) 461, 1219, 1444, 2319, 2367
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 3 cut(s) 214, 304, 2163
BcnI CCSGG 2 cut(s) 1171, 1685
BcoDI GTCTC 6 cut(s) 367, 600, 890, 1396, 1593, 1706
BfaI CTAG 3 cut(s) 210, 429, 2429
BfmI CTRYAG 1 cut(s) 774
BfoI RGCGCY 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 1125
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 2 cut(s) 666, 1299
BisI GCNGC 4 cut(s) 1076, 1079, 2049, 2326
BlpI GCTNAGC 1 cut(s) 1476
BlsI GCNGC 4 cut(s) 1077, 1080, 2050, 2327
Bme1390I CCNGG 7 cut(s) 461, 1171, 1219, 1444, 1685, 2319, 2367
Bme18I GGWCC 2 cut(s) 449, 2283
BmeRI GACNNNNNGTC 1 cut(s) 2126
BmeT110I CYCGRG 2 cut(s) 15, 2377
BmgT120I GGNCC 4 cut(s) 449, 960, 1396, 2283
BmiI GGNNCC 3 cut(s) 317, 693, 757
BmrFI CCNGG 7 cut(s) 461, 1171, 1219, 1444, 1685, 2319, 2367
BmsI GCATC 5 cut(s) 772, 1212, 1276, 1428, 1659
BpiI GAAGAC 1 cut(s) 1544
Bpu1102I GCTNAGC 1 cut(s) 1476
Bpu14I TTCGAA 1 cut(s) 1962
BpuEI CTTGAG 1 cut(s) 2109
BpuMI CCSGG 2 cut(s) 1171, 1685
Bsa29I ATCGAT 2 cut(s) 880, 2178
BsaAI YACGTR 3 cut(s) 267, 409, 2184
BsaBI GATNNNNATC 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 692
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 9 cut(s) 228, 349, 460, 505, 873, 917, 1443, 1793, 2376
BsaWI WCCGGW 1 cut(s) 312
BsaXI ACNNNNNCTCC 6 cut(s) 30, 60, 433, 463, 615, 645
Bsc4I CCNNNNNNNGG 6 cut(s) 92, 93, 506, 752, 957, 1579
Bse118I RCCGGY 1 cut(s) 312
Bse1I ACTGG 3 cut(s) 988, 1877, 2285
Bse21I CCTNAGG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 237
BseBI CCWGG 5 cut(s) 461, 1219, 1444, 2319, 2367
BseCI ATCGAT 2 cut(s) 880, 2178
BseDI CCNNGG 9 cut(s) 228, 349, 460, 505, 873, 917, 1443, 1793, 2376
BseGI GGATG 7 cut(s) 245, 850, 1227, 1278, 1752, 2414, 2441
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 6 cut(s) 92, 93, 506, 752, 957, 1579
BseMII CTCAG 7 cut(s) 148, 831, 1162, 1287, 1391, 1467, 1511
BseNI ACTGG 3 cut(s) 988, 1877, 2285
BseRI GAGGAG 3 cut(s) 588, 1296, 1793
BseXI GCAGC 3 cut(s) 1065, 2060, 2337
BsgI GTGCAG 1 cut(s) 47
Bsh1236I CGCG 2 cut(s) 116, 1824
Bsh1285I CGRYCG 1 cut(s) 884
BshFI GGCC 4 cut(s) 961, 1174, 1397, 2438
BshNI GGYRCC 2 cut(s) 691, 755
BshTI ACCGGT 1 cut(s) 312
BshVI ATCGAT 2 cut(s) 880, 2178
BsiEI CGRYCG 1 cut(s) 884
BsiHKCI CYCGRG 2 cut(s) 15, 2377
BsiSI CCGG 3 cut(s) 313, 1171, 1684
BsiWI CGTACG 1 cut(s) 117
BslFI GGGAC 6 cut(s) 25, 32, 162, 477, 925, 1827
BslI CCNNNNNNNGG 6 cut(s) 92, 93, 506, 752, 957, 1579
BsmAI GTCTC 6 cut(s) 367, 600, 890, 1396, 1593, 1706
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 6 cut(s) 25, 32, 162, 477, 925, 1827
BsnI GGCC 4 cut(s) 961, 1174, 1397, 2438
Bso31I GGTCTC 1 cut(s) 600
BsoBI CYCGRG 2 cut(s) 15, 2377
Bsp119I TTCGAA 1 cut(s) 1962
Bsp1720I GCTNAGC 1 cut(s) 1476
Bsp19I CCATGG 3 cut(s) 505, 873, 917
BspACI CCGC 8 cut(s) 490, 750, 951, 1075, 1108, 1354, 1824, 1950
BspANI GGCC 4 cut(s) 961, 1174, 1397, 2438
BspCNI CTCAG 7 cut(s) 147, 832, 1161, 1288, 1392, 1468, 1512
BspDI ATCGAT 2 cut(s) 880, 2178
BspFNI CGCG 2 cut(s) 116, 1824
BspLI GGNNCC 3 cut(s) 317, 693, 757
BspMI ACCTGC 1 cut(s) 1125
BspPI GGATC 3 cut(s) 885, 885, 1553
BspT104I TTCGAA 1 cut(s) 1962
BspT107I GGYRCC 2 cut(s) 691, 755
BspTNI GGTCTC 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 312
BsrI ACTGG 3 cut(s) 988, 1877, 2285
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 9 cut(s) 228, 349, 460, 505, 873, 917, 1443, 1793, 2376
BssNI GRCGYC 1 cut(s) 692
BssT1I CCWWGG 5 cut(s) 349, 505, 873, 917, 1793
Bst2UI CCWGG 5 cut(s) 461, 1219, 1444, 2319, 2367
Bst4CI ACNGT 6 cut(s) 23, 655, 775, 829, 1819, 2200
Bst6I CTCTTC 2 cut(s) 584, 1167
BstACI GRCGYC 1 cut(s) 692
BstBAI YACGTR 3 cut(s) 267, 409, 2184
BstBI TTCGAA 1 cut(s) 1962
BstC8I GCNNGC 2 cut(s) 562, 789
BstDSI CCRYGG 3 cut(s) 505, 873, 917
BstF5I GGATG 7 cut(s) 245, 850, 1227, 1278, 1752, 2414, 2441
BstFNI CGCG 2 cut(s) 116, 1824
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 1 cut(s) 694
BstMAI GTCTC 6 cut(s) 367, 600, 890, 1396, 1593, 1706
BstMCI CGRYCG 1 cut(s) 884
BstMWI GCNNNNNNNGC 1 cut(s) 1726
BstNI CCWGG 5 cut(s) 461, 1219, 1444, 2319, 2367
BstNSI RCATGY 3 cut(s) 564, 1277, 1947
BstSCI CCNGG 7 cut(s) 459, 1169, 1217, 1442, 1683, 2317, 2365
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 2 cut(s) 116, 1824
BstV1I GCAGC 3 cut(s) 1065, 2060, 2337
BstV2I GAAGAC 1 cut(s) 1544
BstX2I RGATCY 3 cut(s) 666, 1299, 1558
BstYI RGATCY 3 cut(s) 666, 1299, 1558
Bsu15I ATCGAT 2 cut(s) 880, 2178
Bsu36I CCTNAGG 1 cut(s) 91
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 4 cut(s) 961, 1174, 1397, 2438
BsuTUI ATCGAT 2 cut(s) 880, 2178
BtgI CCRYGG 3 cut(s) 505, 873, 917
BtsCI GGATG 7 cut(s) 245, 850, 1227, 1278, 1752, 2414, 2441
BtsIMutI CAGTG 2 cut(s) 28, 981
BveI ACCTGC 1 cut(s) 1125
Cac8I GCNNGC 2 cut(s) 562, 789
CfoI GCGC 1 cut(s) 694
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 4 cut(s) 449, 960, 1396, 2283
ClaI ATCGAT 2 cut(s) 880, 2178
CsiI ACCWGGT 1 cut(s) 2365
Csp6I GTAC 7 cut(s) 118, 424, 457, 856, 922, 1052, 2315
CspAI ACCGGT 1 cut(s) 312
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviQI GTAC 7 cut(s) 118, 424, 457, 856, 922, 1052, 2315
DinI GGCGCC 1 cut(s) 693
DraI TTTAAA 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 2126
EaeI YGGCCR 1 cut(s) 1172
Eam1104I CTCTTC 2 cut(s) 584, 1167
Eam1105I GACNNNNNGTC 1 cut(s) 2126
EarI CTCTTC 2 cut(s) 584, 1167
EciI GGCGGA 1 cut(s) 1343
Eco130I CCWWGG 5 cut(s) 349, 505, 873, 917, 1793
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 449, 2283
Eco57I CTGAAG 2 cut(s) 1556, 2087
Eco72I CACGTG 1 cut(s) 409
Eco81I CCTNAGG 1 cut(s) 91
Eco88I CYCGRG 2 cut(s) 15, 2377
EcoRII CCWGG 5 cut(s) 459, 1217, 1442, 2317, 2365
EcoT14I CCWWGG 5 cut(s) 349, 505, 873, 917, 1793
EcoT22I ATGCAT 1 cut(s) 1227
EgeI GGCGCC 1 cut(s) 693
EheI GGCGCC 1 cut(s) 693
ErhI CCWWGG 5 cut(s) 349, 505, 873, 917, 1793
Esp3I CGTCTC 1 cut(s) 367
FalI AAGNNNNNCTT 2 cut(s) 2118, 2150
FaqI GGGAC 6 cut(s) 25, 32, 162, 477, 925, 1827
FbaI TGATCA 3 cut(s) 214, 304, 2163
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 1076, 1079, 2049, 2326
FokI GGATG 7 cut(s) 232, 857, 1234, 1265, 1739, 2401, 2428
Fsp4HI GCNGC 4 cut(s) 1076, 1079, 2049, 2326
FspBI CTAG 3 cut(s) 210, 429, 2429
GlaI GCGC 1 cut(s) 693
GluI GCNGC 4 cut(s) 1076, 1079, 2049, 2326
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 4 cut(s) 961, 1174, 1397, 2438
HapII CCGG 3 cut(s) 313, 1171, 1684
HhaI GCGC 1 cut(s) 694
Hin1I GRCGYC 1 cut(s) 692
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HincII GTYRAC 2 cut(s) 1500, 2065
HindII GTYRAC 2 cut(s) 1500, 2065
HinfI GANTC 7 cut(s) 13, 379, 465, 624, 1284, 1540, 2218
HpaII CCGG 3 cut(s) 313, 1171, 1684
HphI GGTGA 9 cut(s) 302, 521, 758, 1015, 1459, 1482, 1663, 1798, 2204
Hpy166II GTNNAC 6 cut(s) 76, 1052, 1500, 2065, 2338, 2363
Hpy8I GTNNAC 6 cut(s) 76, 1052, 1500, 2065, 2338, 2363
Hpy99I CGWCG 2 cut(s) 856, 1984
HpyAV CCTTC 6 cut(s) 302, 487, 809, 1320, 1526, 2305
HpyCH4III ACNGT 6 cut(s) 23, 655, 775, 829, 1819, 2200
HpyCH4IV ACGT 6 cut(s) 120, 266, 408, 417, 851, 2183
HpyF10VI GCNNNNNNNGC 1 cut(s) 1726
HpySE526I ACGT 6 cut(s) 120, 266, 408, 417, 851, 2183
Hsp92I GRCGYC 1 cut(s) 692
HspAI GCGC 1 cut(s) 692
KasI GGCGCC 1 cut(s) 691
Ksp22I TGATCA 3 cut(s) 214, 304, 2163
LmnI GCTCC 2 cut(s) 1220, 1952
Lsp1109I GCAGC 3 cut(s) 1065, 2060, 2337
LweI GCATC 5 cut(s) 772, 1212, 1276, 1428, 1659
MabI ACCWGGT 1 cut(s) 2365
MaeI CTAG 3 cut(s) 210, 429, 2429
MaeII ACGT 6 cut(s) 120, 266, 408, 417, 851, 2183
MaeIII GTNAC 3 cut(s) 146, 1813, 2210
MboII GAAGA 9 cut(s) 245, 571, 925, 1091, 1154, 1375, 1516, 1549, 2093
MfeI CAATTG 3 cut(s) 1724, 1897, 2222
MflI RGATCY 3 cut(s) 666, 1299, 1558
MluI ACGCGT 1 cut(s) 114
Mly113I GGCGCC 1 cut(s) 692
MlyI GAGTC 4 cut(s) 7, 459, 618, 1534
MmeI TCCRAC 2 cut(s) 658, 770
Mph1103I ATGCAT 1 cut(s) 1227
MroXI GAANNNNTTC 1 cut(s) 1638
MslI CAYNNNNRTG 5 cut(s) 653, 1028, 1272, 1811, 1973
MspA1I CMGCKG 2 cut(s) 752, 2051
MspI CCGG 3 cut(s) 313, 1171, 1684
MspR9I CCNGG 7 cut(s) 461, 1171, 1219, 1444, 1685, 2319, 2367
MunI CAATTG 3 cut(s) 1724, 1897, 2222
MvaI CCWGG 5 cut(s) 461, 1219, 1444, 2319, 2367
MvnI CGCG 2 cut(s) 116, 1824
MwoI GCNNNNNNNGC 1 cut(s) 1726
NarI GGCGCC 1 cut(s) 692
NciI CCSGG 2 cut(s) 1171, 1685
NcoI CCATGG 3 cut(s) 505, 873, 917
NlaIV GGNNCC 3 cut(s) 317, 693, 757
NmuCI GTSAC 3 cut(s) 146, 1813, 2210
NsiI ATGCAT 1 cut(s) 1227
NspI RCATGY 3 cut(s) 564, 1277, 1947
NspV TTCGAA 1 cut(s) 1962
OliI CACNNNNGTG 2 cut(s) 653, 1811
PaeI GCATGC 1 cut(s) 564
PcsI WCGNNNNNNNCGW 3 cut(s) 414, 552, 886
PdmI GAANNNNTTC 1 cut(s) 1638
PfeI GAWTC 3 cut(s) 379, 1284, 2218
Pfl23II CGTACG 1 cut(s) 117
PflMI CCANNNNNTGG 1 cut(s) 752
PfoI TCCNGGA 2 cut(s) 1217, 1683
PinAI ACCGGT 1 cut(s) 312
PkrI GCNGC 4 cut(s) 1077, 1080, 2050, 2327
Ple19I CGATCG 1 cut(s) 884
PleI GAGTC 4 cut(s) 7, 459, 618, 1534
PluTI GGCGCC 1 cut(s) 695
PmaCI CACGTG 1 cut(s) 409
PmlI CACGTG 1 cut(s) 409
PpsI GAGTC 4 cut(s) 7, 459, 618, 1534
Ppu21I YACGTR 3 cut(s) 267, 409, 2184
PshBI ATTAAT 2 cut(s) 342, 1389
PsiI TTATAA 1 cut(s) 2111
Psp1406I AACGTT 1 cut(s) 417
Psp6I CCWGG 5 cut(s) 459, 1217, 1442, 2317, 2365
PspCI CACGTG 1 cut(s) 409
PspGI CCWGG 5 cut(s) 459, 1217, 1442, 2317, 2365
PspLI CGTACG 1 cut(s) 117
PspN4I GGNNCC 3 cut(s) 317, 693, 757
PspPI GGNCC 4 cut(s) 449, 960, 1396, 2283
PsuI RGATCY 3 cut(s) 666, 1299, 1558
PvuI CGATCG 1 cut(s) 884
PvuII CAGCTG 1 cut(s) 2051
RsaI GTAC 7 cut(s) 119, 425, 458, 857, 923, 1053, 2316
RsaNI GTAC 7 cut(s) 118, 424, 457, 856, 922, 1052, 2315
RseI CAYNNNNRTG 5 cut(s) 653, 1028, 1272, 1811, 1973
SatI GCNGC 4 cut(s) 1076, 1079, 2049, 2326
Sau96I GGNCC 4 cut(s) 449, 960, 1396, 2283
SchI GAGTC 4 cut(s) 7, 459, 618, 1534
ScrFI CCNGG 7 cut(s) 461, 1171, 1219, 1444, 1685, 2319, 2367
SexAI ACCWGGT 1 cut(s) 2365
SfaNI GCATC 5 cut(s) 772, 1212, 1276, 1428, 1659
SfcI CTRYAG 1 cut(s) 774
SfoI GGCGCC 1 cut(s) 693
SfuI TTCGAA 1 cut(s) 1962
SinI GGWCC 2 cut(s) 449, 2283
SmiMI CAYNNNNRTG 5 cut(s) 653, 1028, 1272, 1811, 1973
SmlI CTYRAG 1 cut(s) 2124
SmoI CTYRAG 1 cut(s) 2124
SphI GCATGC 1 cut(s) 564
SsiI CCGC 8 cut(s) 490, 750, 951, 1075, 1108, 1354, 1824, 1950
SspDI GGCGCC 1 cut(s) 691
SspMI CTAG 3 cut(s) 210, 429, 2429
StyD4I CCNGG 7 cut(s) 459, 1169, 1217, 1442, 1683, 2317, 2365
StyI CCWWGG 5 cut(s) 349, 505, 873, 917, 1793
TaaI ACNGT 6 cut(s) 23, 655, 775, 829, 1819, 2200
TaiI ACGT 6 cut(s) 123, 269, 411, 420, 854, 2186
TatI WGTACW 2 cut(s) 423, 1051
TauI GCSGC 1 cut(s) 1078
TfiI GAWTC 3 cut(s) 379, 1284, 2218
TscAI CASTG 2 cut(s) 28, 988
TseFI GTSAC 3 cut(s) 146, 1813, 2210
TseI GCWGC 3 cut(s) 1078, 2048, 2325
Tsp45I GTSAC 3 cut(s) 146, 1813, 2210
TspDTI ATGAA 4 cut(s) 726, 1154, 2000, 2020
TspGWI ACGGA 5 cut(s) 391, 544, 825, 1485, 2206
TspRI CASTG 2 cut(s) 28, 988
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 2 cut(s) 449, 2283
VspI ATTAAT 2 cut(s) 342, 1389
XapI RAATTY 4 cut(s) 129, 170, 732, 1763
XceI RCATGY 3 cut(s) 564, 1277, 1947
XcmI CCANNNNNNNNNTGG 2 cut(s) 810, 1182
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 1638
XspI CTAG 3 cut(s) 210, 429, 2429
Zsp2I ATGCAT 1 cut(s) 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.