pycom01g08810

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003309
Physical Location & Seq
Reverse (-)
9739609 .. 9745534
5926 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1413 bp
ATGACCCCAACATATCGAGTCCAAACTCTTACCCTACACCGCGATGCACCAGATCTCTTTCCGTGTCTGTCCATTTTTCCGGATGAAACGTTCACCAAACCACTTCAACTTCGTCGATTCCACACCATGGACTCGGAATCAGTAATTGAGTTCCTGGGCTGCGTTCCTCTGCTTCAGAGGCTCCCCAGCTCCTCTCTCAAGAAAATCGCCCAGCTCGTCGTTGTCAAACGCTACGAGAGTGGAGAGTATGTTGTTCGTGAAGGTGAAGCAGGCGACGGGGTGTACTTCATATGGGAAGGAGAGGCTGAAGTTACTGGTTCTGTTAATGCCTATGAAGGAAATCAACCTGAGTTCAAATTGAAACGATATGATTATTTTGGTTATGGTTTTGCATCAATTGAATATCAGGCCGATGTGATTGCTTTATCTAAGCTGACTTGCTTTGTGCTACCCCATGAGAATTGTACTCTGCTGCAGCCAAAATCAATCTGGAATGCTGATGATGCCCAAGAGACATGCTCTCTTGTTGAGAATATACTGCATTTGGATCCAATTGAAGTAAACATATTTCGAGGGATTACTTTGCCGGATGCGCCAAGATTCGGACAGGTTTTTGGAGGACAGTTAGTTGGACAGGCATTGGCTGCGGCAACGAAAACTGTTGATTGTCTTAAACTTCTCCATAGTCTGCATGCACATTTTCTGCTTGTTGGGGATTGTAACACACCAATTATATATCAAGTCCATAGAGTACGTGATGGGAAGAGCTTTGCTACTCGAAGAGTGGATGCAATACAAAAGGGGATTAACATTTTCACGTTACTGGCATCATTTAAAAAAGAAGAAGAAGGGTCTGTCCACCAGCAAGCAACAATGCCATCTGTGCCTGCTCCAGATAAACTTCTGTCGATGGAAGAACTACGTGAAAAACGTCTTCTTGATCCACGTCTTCCAAGGACTTATCGAATCAAGGCAGCTGCAAAGAAATCTGTGCCTTGGCCTATAGAGATACGATTTTGTGAGCCTAGCAGCTCAACCAATCAGACTAAATCTGCACCGAGTTTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCTGACGATCAGGCTTTGCATAGATGTGTTGTAGCATATGCTTCAGATTTGATATTTGCTGGTGTAAGTCTGAATCCCCACCGCAGAATTGGAGTTAAGTTGAGTCAAGTTAGTTTGGACCATGCGATATGGTTTCACCGTCAGGTTAGAGCTGATGACTGGTTACTGTATGTGATAGTGAGTCCGATTGCCTATGACACACGTGGATTTGTTTCAGGCCAAATGTTTAACAGACAGGGAGAGCTTATTGTATCATTGACCCAAGAGTCCCTAAACAGGCCAATCAGACCAACTCCGGCTGCCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

471

Amino Acids

52.98

Weight (kDa)

8.46

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 76 - 154 6e-10 Cyclic nucleotide-binding domain
4HBT_3 PF13622 199 - 278 1.4e-17 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 346 - 455 7.4e-22 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 347 - 454 2.5e-26 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1360
AccB7I CCANNNNNTGG 1 cut(s) 127
AccII CGCG 1 cut(s) 42
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 3 cut(s) 40, 647, 1177
AclI AACGTT 1 cut(s) 89
AclWI GGATC 3 cut(s) 542, 555, 935
AcuI CTGAAG 3 cut(s) 158, 327, 1122
AcvI CACGTG 1 cut(s) 1298
AfaI GTAC 3 cut(s) 284, 466, 753
AfiI CCNNNNNNNGG 3 cut(s) 127, 602, 1371
AflIII ACRYGT 1 cut(s) 1295
AgsI TTSAA 5 cut(s) 107, 355, 361, 401, 557
AjiI CACGTC 1 cut(s) 947
AjnI CCWGG 1 cut(s) 153
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 8 cut(s) 189, 214, 433, 768, 977, 1032, 1247, 1339
AluI AGCT 8 cut(s) 189, 214, 433, 768, 977, 1032, 1247, 1339
Alw26I GTCTC 1 cut(s) 506
AlwI GGATC 3 cut(s) 542, 555, 935
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 4 cut(s) 408, 998, 1312, 1373
ApeKI GCWGC 8 cut(s) 159, 472, 475, 644, 974, 977, 1029, 1394
Asp700I GAANNNNTTC 1 cut(s) 1093
AspLEI GCGC 1 cut(s) 595
AspS9I GGNCC 1 cut(s) 1213
AsuHPI GGTGA 3 cut(s) 85, 275, 1223
AvaII GGWCC 1 cut(s) 1213
BamHI GGATCC 1 cut(s) 547
BbrPI CACGTG 1 cut(s) 1298
BbsI GAAGAC 2 cut(s) 926, 941
BbvI GCAGC 8 cut(s) 146, 459, 487, 631, 964, 986, 1041, 1381
BccI CCATC 3 cut(s) 752, 886, 904
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 155
BcoDI GTCTC 1 cut(s) 506
BfaI CTAG 1 cut(s) 1026
BfmI CTRYAG 2 cut(s) 473, 1002
BglII AGATCT 1 cut(s) 52
BisI GCNGC 9 cut(s) 160, 473, 476, 645, 648, 975, 978, 1030, 1395
BlsI GCNGC 9 cut(s) 161, 474, 477, 646, 649, 976, 979, 1031, 1396
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 1 cut(s) 1213
BmgBI CACGTC 1 cut(s) 947
BmgT120I GGNCC 1 cut(s) 1213
BmiI GGNNCC 2 cut(s) 182, 549
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 6 cut(s) 34, 401, 493, 580, 778, 836
BpiI GAAGAC 2 cut(s) 926, 941
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 182
BsaAI YACGTR 3 cut(s) 755, 923, 1298
BsaJI CCNNGG 4 cut(s) 126, 154, 953, 995
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 602, 1371
Bse1I ACTGG 3 cut(s) 319, 828, 1259
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 4 cut(s) 126, 154, 953, 995
BseGI GGATG 3 cut(s) 88, 595, 793
BseLI CCNNNNNNNGG 3 cut(s) 127, 602, 1371
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 3 cut(s) 319, 828, 1259
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 8 cut(s) 146, 459, 487, 631, 964, 986, 1041, 1381
BseYI CCCAGC 2 cut(s) 185, 210
BsgI GTGCAG 1 cut(s) 1038
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 410, 1000, 1314, 1375
BsiSI CCGG 3 cut(s) 80, 587, 1391
BslFI GGGAC 1 cut(s) 1348
BslI CCNNNNNNNGG 3 cut(s) 127, 602, 1371
BsmAI GTCTC 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 1348
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 4 cut(s) 410, 1000, 1314, 1375
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 4 cut(s) 52, 547, 940, 1102
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 3 cut(s) 40, 647, 1177
BspANI GGCC 4 cut(s) 410, 1000, 1314, 1375
BspCNI CTCAG 1 cut(s) 340
BspEI TCCGGA 1 cut(s) 79
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 182, 549
BspMAI CTGCAG 1 cut(s) 477
BspPI GGATC 3 cut(s) 542, 555, 935
BspQI GCTCTTC 1 cut(s) 758
BsrI ACTGG 3 cut(s) 319, 828, 1259
BssECI CCNNGG 4 cut(s) 126, 154, 953, 995
BssMI GATC 4 cut(s) 52, 547, 940, 1102
BssT1I CCWWGG 3 cut(s) 126, 953, 995
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 4 cut(s) 624, 661, 1235, 1263
Bst6I CTCTTC 2 cut(s) 758, 775
BstAPI GCANNNNNTGC 1 cut(s) 644
BstBAI YACGTR 3 cut(s) 755, 923, 1298
BstC8I GCNNGC 4 cut(s) 271, 693, 867, 888
BstDEI CTNAG 3 cut(s) 348, 429, 1404
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 3 cut(s) 88, 595, 793
BstFNI CGCG 1 cut(s) 42
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 4 cut(s) 55, 550, 943, 1105
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 4 cut(s) 52, 547, 940, 1102
BstMWI GCNNNNNNNGC 5 cut(s) 178, 503, 592, 644, 883
BstNI CCWGG 1 cut(s) 155
BstNSI RCATGY 2 cut(s) 519, 695
BstSCI CCNGG 1 cut(s) 153
BstSFI CTRYAG 2 cut(s) 473, 1002
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 8 cut(s) 146, 459, 487, 631, 964, 986, 1041, 1381
BstV2I GAAGAC 2 cut(s) 926, 941
BstX2I RGATCY 2 cut(s) 52, 547
BstYI RGATCY 2 cut(s) 52, 547
BsuRI GGCC 4 cut(s) 410, 1000, 1314, 1375
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 57
BtrI CACGTC 1 cut(s) 947
BtsCI GGATG 3 cut(s) 88, 595, 793
Cac8I GCNNGC 4 cut(s) 271, 693, 867, 888
CfoI GCGC 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 1213
Csp6I GTAC 3 cut(s) 283, 465, 752
CviAII CATG 5 cut(s) 127, 455, 516, 692, 1217
CviQI GTAC 3 cut(s) 283, 465, 752
DdeI CTNAG 3 cut(s) 348, 429, 1404
DpnI GATC 4 cut(s) 54, 549, 942, 1104
DpnII GATC 4 cut(s) 52, 547, 940, 1102
DraI TTTAAA 1 cut(s) 835
DrdI GACNNNNNNGTC 1 cut(s) 1360
DseDI GACNNNNNNGTC 1 cut(s) 1360
Eam1104I CTCTTC 2 cut(s) 758, 775
EarI CTCTTC 2 cut(s) 758, 775
Eco130I CCWWGG 3 cut(s) 126, 953, 995
Eco47I GGWCC 1 cut(s) 1213
Eco57I CTGAAG 3 cut(s) 158, 327, 1122
Eco72I CACGTG 1 cut(s) 1298
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 3 cut(s) 126, 953, 995
ErhI CCWWGG 3 cut(s) 126, 953, 995
FaeI CATG 5 cut(s) 130, 458, 519, 695, 1220
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FaqI GGGAC 1 cut(s) 1348
FatI CATG 5 cut(s) 126, 454, 515, 691, 1216
FauNDI CATATG 2 cut(s) 290, 1132
Fnu4HI GCNGC 9 cut(s) 160, 473, 476, 645, 648, 975, 978, 1030, 1395
FokI GGATG 3 cut(s) 95, 602, 800
Fsp4HI GCNGC 9 cut(s) 160, 473, 476, 645, 648, 975, 978, 1030, 1395
FspBI CTAG 1 cut(s) 1026
GlaI GCGC 1 cut(s) 594
GluI GCNGC 9 cut(s) 160, 473, 476, 645, 648, 975, 978, 1030, 1395
GsaI CCCAGC 2 cut(s) 189, 214
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 4 cut(s) 410, 1000, 1314, 1375
HapII CCGG 3 cut(s) 80, 587, 1391
HhaI GCGC 1 cut(s) 595
Hin1II CATG 5 cut(s) 130, 458, 519, 695, 1220
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HpaII CCGG 3 cut(s) 80, 587, 1391
HphI GGTGA 3 cut(s) 85, 275, 1223
Hpy166II GTNNAC 4 cut(s) 93, 283, 562, 859
Hpy188I TCNGA 9 cut(s) 136, 177, 605, 1044, 1099, 1141, 1167, 1281, 1382
Hpy188III TCNNGA 6 cut(s) 80, 199, 257, 490, 893, 938
Hpy8I GTNNAC 4 cut(s) 93, 283, 562, 859
Hpy99I CGWCG 3 cut(s) 117, 221, 278
HpyAV CCTTC 4 cut(s) 254, 290, 329, 842
HpyCH4III ACNGT 4 cut(s) 624, 661, 1235, 1263
HpyCH4IV ACGT 7 cut(s) 89, 754, 818, 922, 931, 946, 1297
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 503, 592, 644, 883
HpyF3I CTNAG 3 cut(s) 348, 429, 1404
HpySE526I ACGT 7 cut(s) 89, 754, 818, 922, 931, 946, 1297
Hsp92II CATG 5 cut(s) 130, 458, 519, 695, 1220
HspAI GCGC 1 cut(s) 593
Kpn2I TCCGGA 1 cut(s) 79
Kzo9I GATC 4 cut(s) 52, 547, 940, 1102
LguI GCTCTTC 1 cut(s) 758
LmnI GCTCC 3 cut(s) 186, 194, 895
Lsp1109I GCAGC 8 cut(s) 146, 459, 487, 631, 964, 986, 1041, 1381
LweI GCATC 6 cut(s) 34, 401, 493, 580, 778, 836
MaeI CTAG 1 cut(s) 1026
MaeII ACGT 7 cut(s) 89, 754, 818, 922, 931, 946, 1297
MaeIII GTNAC 4 cut(s) 310, 719, 819, 1257
MalI GATC 4 cut(s) 54, 549, 942, 1104
MboI GATC 4 cut(s) 52, 547, 940, 1102
MboII GAAGA 7 cut(s) 775, 792, 854, 857, 926, 926, 941
MfeI CAATTG 2 cut(s) 396, 552
MflI RGATCY 2 cut(s) 52, 547
MluCI AATT 7 cut(s) 144, 356, 396, 460, 552, 729, 1182
MlyI GAGTC 5 cut(s) 27, 125, 1207, 1285, 1370
MmeI TCCRAC 1 cut(s) 610
MnlI CCTC 7 cut(s) 171, 177, 202, 295, 566, 611, 1059
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 1093
MseI TTAA 6 cut(s) 324, 672, 807, 834, 1191, 1323
MslI CAYNNNNRTG 2 cut(s) 42, 1119
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 3 cut(s) 80, 587, 1391
MspR9I CCNGG 1 cut(s) 155
MunI CAATTG 2 cut(s) 396, 552
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 5 cut(s) 178, 503, 592, 644, 883
NcoI CCATGG 1 cut(s) 126
NdeI CATATG 2 cut(s) 290, 1132
NdeII GATC 4 cut(s) 52, 547, 940, 1102
NlaIII CATG 5 cut(s) 130, 458, 519, 695, 1220
NlaIV GGNNCC 2 cut(s) 182, 549
NspI RCATGY 2 cut(s) 519, 695
PaeI GCATGC 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 758
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 928
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 1 cut(s) 1093
PfeI GAWTC 5 cut(s) 117, 137, 600, 966, 1168
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 9 cut(s) 161, 474, 477, 646, 649, 976, 979, 1031, 1396
PleI GAGTC 5 cut(s) 26, 125, 1206, 1284, 1369
PmaCI CACGTG 1 cut(s) 1298
PmlI CACGTG 1 cut(s) 1298
PpsI GAGTC 5 cut(s) 26, 125, 1206, 1284, 1369
Ppu21I YACGTR 3 cut(s) 755, 923, 1298
Psp1406I AACGTT 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 153
PspCI CACGTG 1 cut(s) 1298
PspFI CCCAGC 2 cut(s) 185, 210
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 182, 549
PspPI GGNCC 1 cut(s) 1213
PstI CTGCAG 1 cut(s) 477
PsuI RGATCY 2 cut(s) 52, 547
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 3 cut(s) 284, 466, 753
RsaNI GTAC 3 cut(s) 283, 465, 752
RseI CAYNNNNRTG 2 cut(s) 42, 1119
SapI GCTCTTC 1 cut(s) 758
SaqAI TTAA 6 cut(s) 324, 672, 807, 834, 1191, 1323
SatI GCNGC 9 cut(s) 160, 473, 476, 645, 648, 975, 978, 1030, 1395
Sau3AI GATC 4 cut(s) 52, 547, 940, 1102
Sau96I GGNCC 1 cut(s) 1213
SchI GAGTC 5 cut(s) 27, 125, 1207, 1285, 1370
ScrFI CCNGG 1 cut(s) 155
SfaNI GCATC 6 cut(s) 34, 401, 493, 580, 778, 836
SfcI CTRYAG 2 cut(s) 473, 1002
SinI GGWCC 1 cut(s) 1213
SmiMI CAYNNNNRTG 2 cut(s) 42, 1119
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SphI GCATGC 1 cut(s) 695
Sse9I AATT 7 cut(s) 144, 356, 396, 460, 552, 729, 1182
SsiI CCGC 3 cut(s) 40, 647, 1177
SspMI CTAG 1 cut(s) 1026
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 3 cut(s) 126, 953, 995
TaaI ACNGT 4 cut(s) 624, 661, 1235, 1263
TaiI ACGT 7 cut(s) 92, 757, 821, 925, 934, 949, 1300
TaqI TCGA 6 cut(s) 16, 115, 571, 778, 908, 964
TasI AATT 7 cut(s) 144, 356, 396, 460, 552, 729, 1182
TatI WGTACW 2 cut(s) 282, 464
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 5 cut(s) 117, 137, 600, 966, 1168
Tru1I TTAA 6 cut(s) 324, 672, 807, 834, 1191, 1323
Tru9I TTAA 6 cut(s) 324, 672, 807, 834, 1191, 1323
TseI GCWGC 8 cut(s) 159, 472, 475, 644, 974, 977, 1029, 1394
TspDTI ATGAA 3 cut(s) 99, 277, 348
TspGWI ACGGA 1 cut(s) 51
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 1213
XceI RCATGY 2 cut(s) 519, 695
XcmI CCANNNNNNNNNTGG 2 cut(s) 486, 1181
XmnI GAANNNNTTC 1 cut(s) 1093
XspI CTAG 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.