pycom02g26670

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002225
Physical Location & Seq
Reverse (-)
24475888 .. 24480345
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGGCTCTTGTGAGTCGGACTCAATCTCTCTCTGCCTTCTCTTATTCCTCCACCCATTTCAGGAAACCCCATTCAGCCTCCTCCTTCAACCCCATTTCTTCTTTACGCTTCAACTCCTCTTCTTCCAATTCCTCCTCCTCCTCACCTTTTACCGAGAAGACTTCTGTCCAGAGGTACCAGCGAGACCGCTGGCTTTACCAGAACCAGCCATTGGAATTTCACCAACCCTTACACACCCCCTGCTGCTCTCTCCCGCCCGATTTTAATTCTCTGAGGGAAAACGACATCGCTTTGCAGCTACCCGAGCTCAGGAAGCTGCTTCAGGTGCTCAGAGAAAAGAGGCACACAGAAGGTGACCGAGGCAGTTGTGGGCCCGGCAATGTCTTCCTGGTCGGTACAGGGCCTGGGGACCCTGAGCTCCTCACAATGAAGGCTTACAGAGTAATTCAGACTGCTGACTTGTTGCTGTATGATAGGTTGGTGTCCAATGATGTCTTGGAATTGGTTGCCTCTGGTGCTAGACTTCTATATGTGGGTAAGACTGCTGGGTACCATAGCAGAACCCAGGAAGAGATACATGAATTGTTACTCGGTTTTGCCGAAGCTGGAGCTAATGTTGTGAGACTAAAAGGAGGGGATCCGCTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTCTGCGACAACAAGGAATTGAAGTTAACGTTATTGTTGGAGTATTACTGCTGCTTCAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

247

Amino Acids

27.28

Weight (kDa)

7.06

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 128 - 240 7.4e-28 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 174, 549
AccB1I GGYRCC 2 cut(s) 174, 549
AccB7I CCANNNNNTGG 1 cut(s) 211
AciI CCGC 3 cut(s) 187, 254, 641
AclI AACGTT 1 cut(s) 705
AclWI GGATC 2 cut(s) 632, 645
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 2 cut(s) 305, 716
AfaI GTAC 3 cut(s) 176, 397, 551
AfiI CCNNNNNNNGG 3 cut(s) 60, 211, 309
AgsI TTSAA 3 cut(s) 88, 112, 698
AjnI CCWGG 3 cut(s) 387, 403, 564
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 6 cut(s) 298, 307, 316, 418, 605, 611
AluI AGCT 6 cut(s) 298, 307, 316, 418, 605, 611
Alw21I GWGCWC 3 cut(s) 309, 330, 420
Alw26I GTCTC 2 cut(s) 177, 616
AlwI GGATC 2 cut(s) 632, 645
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 2 cut(s) 371, 401
ApaI GGGCCC 1 cut(s) 375
ApeKI GCWGC 4 cut(s) 243, 295, 316, 727
ApoI RAATTY 1 cut(s) 215
Asp718I GGTACC 2 cut(s) 174, 549
AspS9I GGNCC 4 cut(s) 371, 372, 401, 409
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 3 cut(s) 135, 212, 365
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 375
BamHI GGATCC 1 cut(s) 637
BanI GGYRCC 2 cut(s) 174, 549
BanII GRGCYC 3 cut(s) 309, 375, 420
BbsI GAAGAC 2 cut(s) 164, 376
Bbv12I GWGCWC 3 cut(s) 309, 330, 420
BbvI GCAGC 4 cut(s) 230, 303, 307, 714
BccI CCATC 1 cut(s) 664
BciT130I CCWGG 3 cut(s) 389, 405, 566
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 177, 616
BfaI CTAG 1 cut(s) 519
BisI GCNGC 4 cut(s) 244, 296, 317, 728
BlsI GCNGC 4 cut(s) 245, 297, 318, 729
Bme1390I CCNGG 4 cut(s) 375, 389, 405, 566
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 371, 372, 401, 409
BmiI GGNNCC 6 cut(s) 176, 373, 410, 411, 551, 639
BmrFI CCNGG 4 cut(s) 375, 389, 405, 566
BoxI GACNNNNGTC 1 cut(s) 164
BpiI GAAGAC 2 cut(s) 164, 376
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 2 cut(s) 308, 414
BpuMI CCSGG 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 358, 404, 564
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 211, 309
Bse3DI GCAATG 1 cut(s) 385
BseBI CCWGG 3 cut(s) 389, 405, 566
BseDI CCNNGG 3 cut(s) 358, 404, 564
BseLI CCNNNNNNNGG 3 cut(s) 60, 211, 309
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 4 cut(s) 263, 322, 343, 405
BseRI GAGGAG 7 cut(s) 70, 106, 124, 127, 130, 410, 680
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 4 cut(s) 230, 303, 307, 714
BseYI CCCAGC 1 cut(s) 545
BshFI GGCC 2 cut(s) 373, 403
BshNI GGYRCC 2 cut(s) 174, 549
BsiHKAI GWGCWC 3 cut(s) 309, 330, 420
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 1 cut(s) 375
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 3 cut(s) 60, 211, 309
BsmAI GTCTC 2 cut(s) 177, 616
BsmFI GGGAC 1 cut(s) 422
BsnI GGCC 2 cut(s) 373, 403
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 302
Bsp120I GGGCCC 1 cut(s) 371
Bsp1286I GDGCHC 4 cut(s) 309, 330, 375, 420
Bsp143I GATC 1 cut(s) 637
BspACI CCGC 3 cut(s) 187, 254, 641
BspANI GGCC 2 cut(s) 373, 403
BspCNI CTCAG 4 cut(s) 264, 321, 342, 406
BspLI GGNNCC 6 cut(s) 176, 373, 410, 411, 551, 639
BspPI GGATC 2 cut(s) 632, 645
BspT107I GGYRCC 2 cut(s) 174, 549
BspTNI GGTCTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 358, 404, 564
BssMI GATC 1 cut(s) 637
Bst2UI CCWGG 3 cut(s) 389, 405, 566
Bst6I CTCTTC 2 cut(s) 124, 564
BstC8I GCNNGC 1 cut(s) 191
BstDEI CTNAG 4 cut(s) 272, 308, 329, 414
BstEII GGTNACC 1 cut(s) 353
BstKTI GATC 1 cut(s) 640
BstMAI GTCTC 2 cut(s) 177, 616
BstMBI GATC 1 cut(s) 637
BstMWI GCNNNNNNNGC 4 cut(s) 304, 313, 325, 515
BstNI CCWGG 3 cut(s) 389, 405, 566
BstPAI GACNNNNGTC 1 cut(s) 164
BstPI GGTNACC 1 cut(s) 353
BstSCI CCNGG 4 cut(s) 373, 387, 403, 564
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 230, 303, 307, 714
BstV2I GAAGAC 2 cut(s) 164, 376
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BsuRI GGCC 2 cut(s) 373, 403
BtgZI GCGATG 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 191
CaiI CAGNNNCTG 1 cut(s) 404
Cfr13I GGNCC 4 cut(s) 371, 372, 401, 409
Csp6I GTAC 3 cut(s) 175, 396, 550
CviAII CATG 1 cut(s) 578
CviQI GTAC 3 cut(s) 175, 396, 550
DdeI CTNAG 4 cut(s) 272, 308, 329, 414
DpnI GATC 1 cut(s) 639
DpnII GATC 1 cut(s) 637
Eam1104I CTCTTC 2 cut(s) 124, 564
EarI CTCTTC 2 cut(s) 124, 564
Ecl136II GAGCTC 2 cut(s) 307, 418
Eco24I GRGCYC 3 cut(s) 309, 375, 420
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 409
Eco53kI GAGCTC 2 cut(s) 307, 418
Eco57I CTGAAG 2 cut(s) 305, 716
Eco88I CYCGRG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 353
EcoICRI GAGCTC 2 cut(s) 307, 418
EcoO109I RGGNCCY 2 cut(s) 401, 409
EcoO65I GGTNACC 1 cut(s) 353
EcoRII CCWGG 3 cut(s) 387, 403, 564
EcoT38I GRGCYC 3 cut(s) 309, 375, 420
FaeI CATG 1 cut(s) 581
FaiI YATR 5 cut(s) 471, 529, 531, 555, 579
FaqI GGGAC 1 cut(s) 422
FatI CATG 1 cut(s) 577
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 244, 296, 317, 728
FriOI GRGCYC 3 cut(s) 309, 375, 420
Fsp4HI GCNGC 4 cut(s) 244, 296, 317, 728
FspBI CTAG 1 cut(s) 519
GluI GCNGC 4 cut(s) 244, 296, 317, 728
GsaI CCCAGC 1 cut(s) 549
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 2 cut(s) 373, 403
HapII CCGG 1 cut(s) 375
Hin1II CATG 1 cut(s) 581
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 2 cut(s) 13, 19
HpaI GTTAAC 1 cut(s) 703
HpaII CCGG 1 cut(s) 375
HphI GGTGA 3 cut(s) 135, 212, 365
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 4 cut(s) 18, 273, 332, 450
Hpy188III TCNNGA 3 cut(s) 61, 169, 310
Hpy8I GTNNAC 1 cut(s) 703
HpyAV CCTTC 5 cut(s) 46, 94, 344, 424, 648
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 313, 325, 515
HpyF3I CTNAG 4 cut(s) 272, 308, 329, 414
HpySE526I ACGT 1 cut(s) 705
Hsp92II CATG 1 cut(s) 581
KflI GGGWCCC 1 cut(s) 409
KpnI GGTACC 2 cut(s) 178, 553
KspAI GTTAAC 1 cut(s) 703
Kzo9I GATC 1 cut(s) 637
LmnI GCTCC 2 cut(s) 423, 608
Lsp1109I GCAGC 4 cut(s) 230, 303, 307, 714
MaeI CTAG 1 cut(s) 519
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 2 cut(s) 353, 585
MalI GATC 1 cut(s) 639
MboI GATC 1 cut(s) 637
MboII GAAGA 6 cut(s) 90, 111, 114, 169, 376, 581
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 4 cut(s) 309, 330, 375, 420
MluCI AATT 7 cut(s) 127, 215, 265, 444, 500, 581, 693
MlyI GAGTC 2 cut(s) 13, 22
MmeI TCCRAC 1 cut(s) 694
MseI TTAA 2 cut(s) 264, 702
MspA1I CMGCKG 2 cut(s) 189, 643
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 4 cut(s) 375, 389, 405, 566
MvaI CCWGG 3 cut(s) 389, 405, 566
MwoI GCNNNNNNNGC 4 cut(s) 304, 313, 325, 515
NciI CCSGG 1 cut(s) 375
NdeII GATC 1 cut(s) 637
NlaIII CATG 1 cut(s) 581
NlaIV GGNNCC 6 cut(s) 176, 373, 410, 411, 551, 639
NmuCI GTSAC 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 4 cut(s) 245, 297, 318, 729
PleI GAGTC 2 cut(s) 13, 21
PpsI GAGTC 2 cut(s) 13, 21
PpuMI RGGWCCY 1 cut(s) 409
PshAI GACNNNNGTC 1 cut(s) 164
Psp124BI GAGCTC 2 cut(s) 309, 420
Psp1406I AACGTT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 3 cut(s) 387, 403, 564
PspEI GGTNACC 1 cut(s) 353
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 3 cut(s) 387, 403, 564
PspN4I GGNNCC 6 cut(s) 176, 373, 410, 411, 551, 639
PspOMI GGGCCC 1 cut(s) 371
PspPI GGNCC 4 cut(s) 371, 372, 401, 409
PspPPI RGGWCCY 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 637
RsaI GTAC 3 cut(s) 176, 397, 551
RsaNI GTAC 3 cut(s) 175, 396, 550
SacI GAGCTC 2 cut(s) 309, 420
SaqAI TTAA 2 cut(s) 264, 702
SatI GCNGC 4 cut(s) 244, 296, 317, 728
Sau3AI GATC 1 cut(s) 637
Sau96I GGNCC 4 cut(s) 371, 372, 401, 409
SchI GAGTC 2 cut(s) 13, 22
ScrFI CCNGG 4 cut(s) 375, 389, 405, 566
SduI GDGCHC 4 cut(s) 309, 330, 375, 420
SinI GGWCC 1 cut(s) 409
Sse9I AATT 7 cut(s) 127, 215, 265, 444, 500, 581, 693
SsiI CCGC 3 cut(s) 187, 254, 641
SspMI CTAG 1 cut(s) 519
SstI GAGCTC 2 cut(s) 309, 420
StyD4I CCNGG 4 cut(s) 373, 387, 403, 564
TaiI ACGT 1 cut(s) 708
TaqII GACCGA 1 cut(s) 372
TasI AATT 7 cut(s) 127, 215, 265, 444, 500, 581, 693
Tru1I TTAA 2 cut(s) 264, 702
Tru9I TTAA 2 cut(s) 264, 702
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 4 cut(s) 243, 295, 316, 727
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 2 cut(s) 443, 594
Van91I CCANNNNNTGG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 1 cut(s) 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XspI CTAG 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.