pycom05g10650

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002089
Physical Location & Seq
Forward (+)
14105331 .. 14107756
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGACTTCTGAATCCATAGTTCACAGTCATGAAGCAGACGAAAAACACGTTGATGCCATGAACTGGGATAAACTGGAATTTGGGGTAATTCCAACCGATTACATGTACATTATGATATGTTCCGATGGAGAGAATTTTTCACAAGGCCATCTCGCTCCCTATAGAAAGATTGAGCTCAGCCCATTTGCTGGAATCTTGAATTATGGACAGGGAATATTAGAAGGTCTCAAGGCAATCAGGAAGAAAGATGGTCGCATTCAGCTCTTTCGTCCAGAAGAGAACGCTCTAAGAATGAAGATGGGTGCAGAGAGATTGTGCATGCCATGTCCATCTACTGAGCAATTTCTTGCTGCTTTAAAACAAGCAGTCCTTGCCAACAAGCGTTGGGTACCACCTTCAGGGAAAGGAGTATTGTATATTAGGCCATTGCTCATTGGAAGTGGTCCTGTTCTTGGCTTGGGGTCAGCAACAGAGTACACATTCCTAATATTTGCTACTCCAATTGGTAGTTATCACAAGAGTGGCAGCACCATGAATTTGTACATTGAGAATGAGGTTCGTAGGGCCACTCCTGGTGGAACTGGAGGCATCAAAAGCATCACCAACTATTCACCAATTTTTAGAACAGTACAAAAGGCTAGGGCCAAAGGGTTCACTGACGTCCTCTTTCTGGATGCAGCCACTGGAAAAAATATTGAGGAGTGTTCTTCATCTAATATTTTCATTGTGAAGGATAATGTGATTTTAACTTCACCAACCAATGGAACTGTCCTTCCAGGGATCACAAGGAAAAGCATCATAGAAATTGCTCTCCATCTCAATTACGTGGTGGAGGAACGTGACGTTTCACCAGAGGATGTGTTGGCTGCTGATGAAGTTTTCTGTACTGGGACTGCTGTGGATGTCATTGCCGTTTCTAGCATAACATATCGTGACAAAAGGGTTGAATACAGAAGAGGGAAAGAAACTGTGTTTTGCAAACTGCGTGAAATGCTTACTGAGATTCAAACAGGTTTGATTGAGGACAAAATGGGGTGGACCTTGCTCGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

352

Amino Acids

38.88

Weight (kDa)

6.35

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 79 - 308 1.2e-36 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 663
Acc65I GGTACC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 388
AccB7I CCANNNNNTGG 3 cut(s) 63, 188, 761
AclWI GGATC 1 cut(s) 788
AcsI RAATTY 3 cut(s) 77, 133, 535
AcuI CTGAAG 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 660
AfaI GTAC 6 cut(s) 107, 390, 476, 542, 630, 886
AfiI CCNNNNNNNGG 6 cut(s) 63, 188, 398, 452, 670, 761
AflIII ACRYGT 2 cut(s) 46, 102
AgsI TTSAA 3 cut(s) 199, 947, 1007
AjnI CCWGG 2 cut(s) 571, 775
AleI CACNNNNGTG 1 cut(s) 519
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
AluBI AGCT 2 cut(s) 175, 262
AluI AGCT 2 cut(s) 175, 262
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 1 cut(s) 788
AlwNI CAGNNNCTG 1 cut(s) 683
AoxI GGCC 4 cut(s) 145, 422, 564, 642
ApeKI GCWGC 4 cut(s) 350, 525, 677, 866
ApoI RAATTY 3 cut(s) 77, 133, 535
Asp718I GGTACC 1 cut(s) 388
AspS9I GGNCC 4 cut(s) 443, 564, 642, 1038
AsuHPI GGTGA 4 cut(s) 592, 603, 744, 840
AvaII GGWCC 2 cut(s) 443, 1038
BanI GGYRCC 1 cut(s) 388
BanII GRGCYC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 4 cut(s) 337, 537, 689, 853
BccI CCATC 6 cut(s) 119, 156, 242, 292, 337, 822
BceAI ACGGC 1 cut(s) 896
BciT130I CCWGG 2 cut(s) 573, 777
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 2 cut(s) 639, 918
BfmI CTRYAG 1 cut(s) 160
BisI GCNGC 4 cut(s) 351, 526, 678, 867
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 4 cut(s) 352, 527, 679, 868
Bme1390I CCNGG 2 cut(s) 573, 777
Bme18I GGWCC 2 cut(s) 443, 1038
BmgT120I GGNCC 4 cut(s) 443, 564, 642, 1038
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 573, 777
BmrI ACTGGG 2 cut(s) 73, 897
BmsI GCATC 5 cut(s) 43, 597, 606, 664, 804
BmuI ACTGGG 2 cut(s) 73, 897
BpmI CTGGAG 1 cut(s) 603
Bpu1102I GCTNAGC 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 212
BsaAI YACGTR 1 cut(s) 826
BsaHI GRCGYC 1 cut(s) 660
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 776
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 188, 398, 452, 670, 761
Bse1I ACTGG 5 cut(s) 68, 78, 586, 688, 892
Bse3DI GCAATG 2 cut(s) 425, 906
BseBI CCWGG 2 cut(s) 573, 777
BseDI CCNNGG 1 cut(s) 776
BseGI GGATG 3 cut(s) 679, 862, 907
BseLI CCNNNNNNNGG 6 cut(s) 63, 188, 398, 452, 670, 761
BseMI GCAATG 2 cut(s) 425, 906
BseMII CTCAG 3 cut(s) 190, 327, 990
BseNI ACTGG 5 cut(s) 68, 78, 586, 688, 892
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 4 cut(s) 337, 537, 689, 853
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 4 cut(s) 147, 424, 566, 644
BshNI GGYRCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 177
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 6 cut(s) 63, 188, 398, 452, 670, 761
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 4 cut(s) 147, 424, 566, 644
Bso31I GGTCTC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 177
Bsp1407I TGTACA 2 cut(s) 105, 540
Bsp143I GATC 1 cut(s) 780
Bsp1720I GCTNAGC 1 cut(s) 176
BspANI GGCC 4 cut(s) 147, 424, 566, 644
BspCNI CTCAG 3 cut(s) 189, 328, 991
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 390
BspPI GGATC 1 cut(s) 788
BspT107I GGYRCC 1 cut(s) 388
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 2 cut(s) 425, 906
BsrGI TGTACA 2 cut(s) 105, 540
BsrI ACTGG 5 cut(s) 68, 78, 586, 688, 892
BssECI CCNNGG 1 cut(s) 776
BssMI GATC 1 cut(s) 780
BssNI GRCGYC 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 573, 777
Bst4CI ACNGT 4 cut(s) 26, 628, 769, 970
Bst6I CTCTTC 2 cut(s) 270, 949
BstACI GRCGYC 1 cut(s) 660
BstAPI GCANNNNNTGC 1 cut(s) 371
BstAUI TGTACA 2 cut(s) 105, 540
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 4 cut(s) 176, 287, 336, 999
BstF5I GGATG 3 cut(s) 679, 862, 907
BstKTI GATC 1 cut(s) 783
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 1 cut(s) 780
BstMWI GCNNNNNNNGC 3 cut(s) 371, 594, 991
BstNI CCWGG 2 cut(s) 573, 777
BstNSI RCATGY 2 cut(s) 106, 322
BstSCI CCNGG 2 cut(s) 571, 775
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 4 cut(s) 337, 537, 689, 853
BsuRI GGCC 4 cut(s) 147, 424, 566, 644
BtsCI GGATG 3 cut(s) 679, 862, 907
BtsIMutI CAGTG 2 cut(s) 654, 681
Cac8I GCNNGC 1 cut(s) 320
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 1 cut(s) 28
Cfr13I GGNCC 4 cut(s) 443, 564, 642, 1038
Csp6I GTAC 6 cut(s) 106, 389, 475, 541, 629, 885
CviAII CATG 6 cut(s) 29, 58, 103, 319, 324, 532
CviQI GTAC 6 cut(s) 106, 389, 475, 541, 629, 885
DdeI CTNAG 4 cut(s) 176, 287, 336, 999
DpnI GATC 1 cut(s) 782
DpnII GATC 1 cut(s) 780
DraI TTTAAA 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 270, 949
EarI CTCTTC 2 cut(s) 270, 949
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 443, 1038
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 571, 775
EcoT38I GRGCYC 1 cut(s) 177
FaeI CATG 6 cut(s) 32, 61, 106, 322, 327, 535
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 1 cut(s) 904
FatI CATG 6 cut(s) 28, 57, 102, 318, 323, 531
Fnu4HI GCNGC 4 cut(s) 351, 526, 678, 867
FokI GGATG 3 cut(s) 686, 869, 914
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 4 cut(s) 351, 526, 678, 867
FspBI CTAG 2 cut(s) 639, 918
GluI GCNGC 4 cut(s) 351, 526, 678, 867
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 4 cut(s) 147, 424, 566, 644
Hin1I GRCGYC 1 cut(s) 660
Hin1II CATG 6 cut(s) 32, 61, 106, 322, 327, 535
HinfI GANTC 3 cut(s) 11, 192, 1003
HphI GGTGA 4 cut(s) 592, 603, 744, 840
Hpy166II GTNNAC 4 cut(s) 22, 477, 654, 1038
Hpy188I TCNGA 2 cut(s) 10, 124
Hpy188III TCNNGA 6 cut(s) 29, 196, 238, 272, 671, 932
Hpy8I GTNNAC 4 cut(s) 22, 477, 654, 1038
HpyAV CCTTC 4 cut(s) 215, 405, 724, 782
HpyCH4III ACNGT 4 cut(s) 26, 628, 769, 970
HpyCH4IV ACGT 5 cut(s) 48, 660, 825, 838, 843
HpyCH4V TGCA 4 cut(s) 305, 318, 677, 978
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 594, 991
HpyF3I CTNAG 4 cut(s) 176, 287, 336, 999
HpySE526I ACGT 5 cut(s) 48, 660, 825, 838, 843
Hsp92I GRCGYC 1 cut(s) 660
Hsp92II CATG 6 cut(s) 32, 61, 106, 322, 327, 535
KpnI GGTACC 1 cut(s) 392
Kzo9I GATC 1 cut(s) 780
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 4 cut(s) 337, 537, 689, 853
LweI GCATC 5 cut(s) 43, 597, 606, 664, 804
MaeI CTAG 2 cut(s) 639, 918
MaeII ACGT 5 cut(s) 48, 660, 825, 838, 843
MaeIII GTNAC 2 cut(s) 839, 932
MalI GATC 1 cut(s) 782
MboI GATC 1 cut(s) 780
MboII GAAGA 5 cut(s) 253, 287, 307, 699, 966
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 8 cut(s) 547, 578, 674, 691, 826, 847, 950, 1015
MseI TTAA 2 cut(s) 356, 746
MslI CAYNNNNRTG 3 cut(s) 27, 51, 519
MspR9I CCNGG 2 cut(s) 573, 777
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 255
MvaI CCWGG 2 cut(s) 573, 777
MwoI GCNNNNNNNGC 3 cut(s) 371, 594, 991
NdeII GATC 1 cut(s) 780
NlaIII CATG 6 cut(s) 32, 61, 106, 322, 327, 535
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 2 cut(s) 839, 932
NspI RCATGY 2 cut(s) 106, 322
OliI CACNNNNGTG 1 cut(s) 519
PaeI GCATGC 1 cut(s) 322
PagI TCATGA 1 cut(s) 28
PciI ACATGT 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 255
PfeI GAWTC 3 cut(s) 11, 192, 1003
PflMI CCANNNNNTGG 3 cut(s) 63, 188, 761
PkrI GCNGC 4 cut(s) 352, 527, 679, 868
Ppu21I YACGTR 1 cut(s) 826
PscI ACATGT 1 cut(s) 102
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 571, 775
PspGI CCWGG 2 cut(s) 571, 775
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 4 cut(s) 443, 564, 642, 1038
PstNI CAGNNNCTG 1 cut(s) 683
RsaI GTAC 6 cut(s) 107, 390, 476, 542, 630, 886
RsaNI GTAC 6 cut(s) 106, 389, 475, 541, 629, 885
RseI CAYNNNNRTG 3 cut(s) 27, 51, 519
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 356, 746
SatI GCNGC 4 cut(s) 351, 526, 678, 867
Sau3AI GATC 1 cut(s) 780
Sau96I GGNCC 4 cut(s) 443, 564, 642, 1038
ScrFI CCNGG 2 cut(s) 573, 777
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 5 cut(s) 43, 597, 606, 664, 804
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 2 cut(s) 443, 1038
SmiMI CAYNNNNRTG 3 cut(s) 27, 51, 519
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SphI GCATGC 1 cut(s) 322
SspI AATATT 4 cut(s) 216, 489, 694, 718
SspMI CTAG 2 cut(s) 639, 918
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 571, 775
TaaI ACNGT 4 cut(s) 26, 628, 769, 970
TaiI ACGT 5 cut(s) 51, 663, 828, 841, 846
TatI WGTACW 5 cut(s) 105, 474, 540, 628, 884
TfiI GAWTC 3 cut(s) 11, 192, 1003
Tru1I TTAA 2 cut(s) 356, 746
Tru9I TTAA 2 cut(s) 356, 746
TscAI CASTG 2 cut(s) 661, 688
TseFI GTSAC 2 cut(s) 839, 932
TseI GCWGC 4 cut(s) 350, 525, 677, 866
Tsp45I GTSAC 2 cut(s) 839, 932
TspDTI ATGAA 7 cut(s) 45, 74, 308, 548, 699, 712, 888
TspRI CASTG 2 cut(s) 661, 688
Van91I CCANNNNNTGG 3 cut(s) 63, 188, 761
VpaK11BI GGWCC 2 cut(s) 443, 1038
XapI RAATTY 3 cut(s) 77, 133, 535
XceI RCATGY 2 cut(s) 106, 322
XspI CTAG 2 cut(s) 639, 918
ZraI GACGTC 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.