pycom07g00280

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002225
Physical Location & Seq
Forward (+)
284442 .. 286861
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
ATGGCTCTTGTGAGTCGGATTCAGTCTCTCTCTGCCTTCTCTTCTTCCTCCACCCATTTCAGAAAACCCCACTCCTCCTCCTCCTTCAACCCCATTTCTTCATTACGCTTCAACTCCACTTCCTCCAATTCCTCCTCCTCACCTTTTACAGAGAAGGCTTCTATCGAGAGATACCAGCGAGACCACTGGCTCTACAAAAACCAACATTCAGAATCCGACCAACCCTTACCCACTCCCTGCTGCTCTCTCCCGCCCGATTTTAATTCCTTGAGGGAAAACGACATCGCTTTGCAGCTACCCGAGCTCAGGAACCTGCTTCAGGTGCTCAGAGAAAAGAGGCAGACTCAAGGTGATCGAGGCGGCTGCGGGCCCGGGAATGTCTTCTTGGTGGGGACGGGGCCTGGGGACCCCGAGCTCCTGACAATGAAGGCTTACAGAGTTATTCAGACTGCTGATTTGTTGCTCTATGATAGGTTGGTGTCCAATGATGTCTTAGATTTGGTTGGCTCTGGTGCTAGACTTCTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAATTGTTACTGAGTTTTGCCGAAGCTGGAGCTAATGTTGTGAGGCTCAAAGGAGGGGATCCGCTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTCTGCGGCAACAAGGAATTGAAGTTAACGTTATTCCAGGTATTACTGCTGCTTCAGGGATAGCAGCAAATCTAGGGATTCCGTTAACTCATCGAGGTGTCTCAAATAGTGTTAGATTTCTCACCGGCCACTCCAGGAAGGGAGGAACAGATCCTCTGTTTGTGGCAGAGAATGCAGCTGACCCTGATTCGACCTTGGTGGTTTACATGGGTTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTGGTGCATCATGGTCTACCGCCAAATACACCAGCTGTTGCAGTTGAGCGAGGGACCACACCTCAACAACGCATGGTTTTTGCAGAACTGAAGGATGTTGCAGATGAAATTGTATCAGCAGAGTTAGTATCACCAACGTTGATCATCATTGGGAAAGTAGTGGCACTTTCACCGTTGTGGCCATATGCTTCAAAGGAAGTATCCTGTCTTGTAGAAACACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

380

Amino Acids

41.0

Weight (kDa)

6.22

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 127 - 338 1.2e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 321
Acc65I GGTACC 1 cut(s) 546
AccB1I GGYRCC 1 cut(s) 546
AccI GTMKAC 2 cut(s) 890, 931
AciI CCGC 6 cut(s) 251, 360, 366, 638, 679, 935
AclI AACGTT 2 cut(s) 702, 1052
AclWI GGATC 3 cut(s) 629, 642, 818
AcoI YGGCCR 2 cut(s) 799, 1094
AcuI CTGAAG 3 cut(s) 302, 711, 1025
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 2 cut(s) 306, 319
AgsI TTSAA 4 cut(s) 88, 112, 695, 1107
AjnI CCWGG 4 cut(s) 400, 561, 709, 806
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AleI CACNNNNGTG 1 cut(s) 1090
AluBI AGCT 7 cut(s) 295, 304, 415, 602, 608, 851, 950
AluI AGCT 7 cut(s) 295, 304, 415, 602, 608, 851, 950
Alw21I GWGCWC 3 cut(s) 306, 327, 417
Alw26I GTCTC 3 cut(s) 30, 174, 778
AlwI GGATC 3 cut(s) 629, 642, 818
Ama87I CYCGRG 3 cut(s) 299, 371, 410
AoxI GGCC 4 cut(s) 368, 398, 799, 1094
ApaI GGGCCC 1 cut(s) 372
ApeKI GCWGC 6 cut(s) 240, 292, 363, 722, 737, 848
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
Asp700I GAANNNNTTC 1 cut(s) 380
Asp718I GGTACC 1 cut(s) 546
AspS9I GGNCC 5 cut(s) 368, 369, 398, 406, 969
AsuC2I CCSGG 2 cut(s) 372, 373
AsuHPI GGTGA 5 cut(s) 132, 362, 787, 1038, 1077
AvaI CYCGRG 3 cut(s) 299, 371, 410
AvaII GGWCC 2 cut(s) 406, 969
BaeGI GKGCMC 1 cut(s) 372
BalI TGGCCA 1 cut(s) 1096
BamHI GGATCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 3 cut(s) 306, 372, 417
BbsI GAAGAC 1 cut(s) 373
Bbv12I GWGCWC 3 cut(s) 306, 327, 417
BbvI GCAGC 6 cut(s) 227, 304, 350, 709, 749, 860
BccI CCATC 1 cut(s) 661
BcgI CGANNNNNNTGC 2 cut(s) 345, 379
BciT130I CCWGG 4 cut(s) 402, 563, 711, 808
BciVI GTATCC 1 cut(s) 1126
BclI TGATCA 1 cut(s) 1056
BcnI CCSGG 2 cut(s) 372, 373
BcoDI GTCTC 3 cut(s) 30, 174, 778
BfaI CTAG 2 cut(s) 516, 746
BfmI CTRYAG 1 cut(s) 1136
BfuAI ACCTGC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 1126
BisI GCNGC 8 cut(s) 241, 293, 361, 364, 680, 723, 738, 849
BlsI GCNGC 8 cut(s) 242, 294, 362, 365, 681, 724, 739, 850
Bme1390I CCNGG 6 cut(s) 372, 373, 402, 563, 711, 808
Bme18I GGWCC 2 cut(s) 406, 969
BmeT110I CYCGRG 3 cut(s) 299, 371, 410
BmgT120I GGNCC 5 cut(s) 368, 369, 398, 406, 969
BmiI GGNNCC 8 cut(s) 311, 370, 399, 407, 408, 548, 636, 970
BmrFI CCNGG 6 cut(s) 372, 373, 402, 563, 711, 808
BmsI GCATC 1 cut(s) 931
BpiI GAAGAC 1 cut(s) 373
BplI GAGNNNNNCTC 2 cut(s) 328, 360
BpmI CTGGAG 2 cut(s) 624, 790
Bpu10I CCTNAGC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 289, 330
BpuMI CCSGG 2 cut(s) 372, 373
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 4 cut(s) 371, 401, 561, 867
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 597, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 319
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 1 cut(s) 191
BseBI CCWGG 4 cut(s) 402, 563, 711, 808
BseDI CCNNGG 4 cut(s) 371, 401, 561, 867
BseGI GGATG 1 cut(s) 1015
BseLI CCNNNNNNNGG 2 cut(s) 306, 319
BseMII CTCAG 3 cut(s) 319, 340, 578
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 7 cut(s) 64, 67, 70, 124, 127, 581, 677
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 6 cut(s) 227, 304, 350, 709, 749, 860
BseYI CCCAGC 1 cut(s) 542
BshFI GGCC 4 cut(s) 370, 400, 801, 1096
BshNI GGYRCC 1 cut(s) 546
BsiHKAI GWGCWC 3 cut(s) 306, 327, 417
BsiHKCI CYCGRG 3 cut(s) 299, 371, 410
BsiSI CCGG 2 cut(s) 372, 798
BslFI GGGAC 3 cut(s) 406, 419, 982
BslI CCNNNNNNNGG 2 cut(s) 306, 319
BsmAI GTCTC 3 cut(s) 30, 174, 778
BsmFI GGGAC 3 cut(s) 406, 419, 982
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 4 cut(s) 370, 400, 801, 1096
Bso31I GGTCTC 1 cut(s) 174
BsoBI CYCGRG 3 cut(s) 299, 371, 410
Bsp120I GGGCCC 1 cut(s) 368
Bsp1286I GDGCHC 4 cut(s) 306, 327, 372, 417
Bsp143I GATC 4 cut(s) 352, 634, 823, 1056
BspACI CCGC 6 cut(s) 251, 360, 366, 638, 679, 935
BspANI GGCC 4 cut(s) 370, 400, 801, 1096
BspCNI CTCAG 3 cut(s) 318, 339, 579
BspLI GGNNCC 8 cut(s) 311, 370, 399, 407, 408, 548, 636, 970
BspMI ACCTGC 1 cut(s) 321
BspPI GGATC 3 cut(s) 629, 642, 818
BspT107I GGYRCC 1 cut(s) 546
BspTNI GGTCTC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 4 cut(s) 371, 401, 561, 867
BssMI GATC 4 cut(s) 352, 634, 823, 1056
BssT1I CCWWGG 1 cut(s) 867
Bst2UI CCWGG 4 cut(s) 402, 563, 711, 808
Bst4CI ACNGT 1 cut(s) 1089
Bst6I CTCTTC 1 cut(s) 46
BstAPI GCANNNNNTGC 1 cut(s) 845
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 4 cut(s) 305, 326, 493, 587
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 1015
BstKTI GATC 4 cut(s) 355, 637, 826, 1059
BstMAI GTCTC 3 cut(s) 30, 174, 778
BstMBI GATC 4 cut(s) 352, 634, 823, 1056
BstMWI GCNNNNNNNGC 3 cut(s) 301, 322, 845
BstNI CCWGG 4 cut(s) 402, 563, 711, 808
BstSCI CCNGG 6 cut(s) 370, 371, 400, 561, 709, 806
BstSFI CTRYAG 1 cut(s) 1136
BstSLI GKGCMC 1 cut(s) 372
BstV1I GCAGC 6 cut(s) 227, 304, 350, 709, 749, 860
BstV2I GAAGAC 1 cut(s) 373
BstX2I RGATCY 2 cut(s) 634, 823
BstYI RGATCY 2 cut(s) 634, 823
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 4 cut(s) 370, 400, 801, 1096
BtgZI GCGATG 1 cut(s) 268
BtsCI GGATG 1 cut(s) 1015
BtsIMutI CAGTG 1 cut(s) 184
BveI ACCTGC 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 368
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 5 cut(s) 368, 369, 398, 406, 969
Cfr9I CCCGGG 1 cut(s) 371
Csp6I GTAC 1 cut(s) 547
CviAII CATG 4 cut(s) 575, 880, 926, 988
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 4 cut(s) 305, 326, 493, 587
DpnI GATC 4 cut(s) 354, 636, 825, 1058
DpnII GATC 4 cut(s) 352, 634, 823, 1056
EaeI YGGCCR 2 cut(s) 799, 1094
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Ecl136II GAGCTC 2 cut(s) 304, 415
Eco130I CCWWGG 1 cut(s) 867
Eco24I GRGCYC 3 cut(s) 306, 372, 417
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 406, 969
Eco53kI GAGCTC 2 cut(s) 304, 415
Eco57I CTGAAG 3 cut(s) 302, 711, 1025
Eco88I CYCGRG 3 cut(s) 299, 371, 410
EcoICRI GAGCTC 2 cut(s) 304, 415
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO109I RGGNCCY 2 cut(s) 398, 406
EcoRII CCWGG 4 cut(s) 400, 561, 709, 806
EcoT14I CCWWGG 1 cut(s) 867
EcoT38I GRGCYC 3 cut(s) 306, 372, 417
ErhI CCWWGG 1 cut(s) 867
FaeI CATG 4 cut(s) 578, 883, 929, 991
FaqI GGGAC 3 cut(s) 406, 419, 982
FatI CATG 4 cut(s) 574, 879, 925, 987
FauI CCCGC 2 cut(s) 258, 359
FauNDI CATATG 1 cut(s) 1099
FbaI TGATCA 1 cut(s) 1056
FblI GTMKAC 2 cut(s) 890, 931
Fnu4HI GCNGC 8 cut(s) 241, 293, 361, 364, 680, 723, 738, 849
FokI GGATG 1 cut(s) 1022
FriOI GRGCYC 3 cut(s) 306, 372, 417
Fsp4HI GCNGC 8 cut(s) 241, 293, 361, 364, 680, 723, 738, 849
FspBI CTAG 2 cut(s) 516, 746
GluI GCNGC 8 cut(s) 241, 293, 361, 364, 680, 723, 738, 849
GsaI CCCAGC 1 cut(s) 546
GsuI CTGGAG 2 cut(s) 624, 790
HaeIII GGCC 4 cut(s) 370, 400, 801, 1096
HapII CCGG 2 cut(s) 372, 798
Hin1II CATG 4 cut(s) 578, 883, 929, 991
HincII GTYRAC 3 cut(s) 700, 759, 891
HindII GTYRAC 3 cut(s) 700, 759, 891
HinfI GANTC 7 cut(s) 13, 19, 212, 343, 751, 860, 892
HpaI GTTAAC 2 cut(s) 700, 759
HpaII CCGG 2 cut(s) 372, 798
HphI GGTGA 5 cut(s) 132, 362, 787, 1038, 1077
Hpy166II GTNNAC 5 cut(s) 700, 759, 877, 891, 932
Hpy188I TCNGA 6 cut(s) 18, 62, 211, 217, 329, 447
Hpy188III TCNNGA 3 cut(s) 166, 307, 418
Hpy8I GTNNAC 5 cut(s) 700, 759, 877, 891, 932
HpyAV CCTTC 8 cut(s) 46, 94, 148, 421, 645, 805, 909, 1000
HpyCH4III ACNGT 1 cut(s) 1089
HpyCH4IV ACGT 2 cut(s) 702, 1052
HpyCH4V TGCA 6 cut(s) 292, 848, 922, 956, 998, 1016
HpyF10VI GCNNNNNNNGC 3 cut(s) 301, 322, 845
HpyF3I CTNAG 4 cut(s) 305, 326, 493, 587
HpySE526I ACGT 2 cut(s) 702, 1052
Hsp92II CATG 4 cut(s) 578, 883, 929, 991
KflI GGGWCCC 1 cut(s) 406
KpnI GGTACC 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 1056
KspAI GTTAAC 2 cut(s) 700, 759
Kzo9I GATC 4 cut(s) 352, 634, 823, 1056
LmnI GCTCC 2 cut(s) 420, 605
Lsp1109I GCAGC 6 cut(s) 227, 304, 350, 709, 749, 860
LweI GCATC 1 cut(s) 931
MaeI CTAG 2 cut(s) 516, 746
MaeII ACGT 2 cut(s) 702, 1052
MaeIII GTNAC 1 cut(s) 582
MalI GATC 4 cut(s) 354, 636, 825, 1058
MboI GATC 4 cut(s) 352, 634, 823, 1056
MboII GAAGA 4 cut(s) 33, 36, 90, 373
MflI RGATCY 2 cut(s) 634, 823
MhlI GDGCHC 4 cut(s) 306, 327, 372, 417
MlsI TGGCCA 1 cut(s) 1096
MluCI AATT 5 cut(s) 127, 262, 578, 690, 1023
MluNI TGGCCA 1 cut(s) 1096
MlyI GAGTC 3 cut(s) 22, 337, 886
MmeI TCCRAC 1 cut(s) 240
Mox20I TGGCCA 1 cut(s) 1096
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 1096
MseI TTAA 3 cut(s) 261, 699, 758
MslI CAYNNNNRTG 2 cut(s) 768, 1090
Msp20I TGGCCA 1 cut(s) 1096
MspA1I CMGCKG 3 cut(s) 640, 851, 950
MspI CCGG 2 cut(s) 372, 798
MspR9I CCNGG 6 cut(s) 372, 373, 402, 563, 711, 808
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 4 cut(s) 402, 563, 711, 808
MwoI GCNNNNNNNGC 3 cut(s) 301, 322, 845
NciI CCSGG 2 cut(s) 372, 373
NdeI CATATG 1 cut(s) 1099
NdeII GATC 4 cut(s) 352, 634, 823, 1056
NlaIII CATG 4 cut(s) 578, 883, 929, 991
NlaIV GGNNCC 8 cut(s) 311, 370, 399, 407, 408, 548, 636, 970
OliI CACNNNNGTG 1 cut(s) 1090
PctI GAATGC 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 4 cut(s) 19, 212, 751, 860
PfoI TCCNGGA 1 cut(s) 806
PkrI GCNGC 8 cut(s) 242, 294, 362, 365, 681, 724, 739, 850
PleI GAGTC 3 cut(s) 21, 337, 886
PpsI GAGTC 3 cut(s) 21, 337, 886
PpuMI RGGWCCY 1 cut(s) 406
Psp124BI GAGCTC 2 cut(s) 306, 417
Psp1406I AACGTT 2 cut(s) 702, 1052
Psp5II RGGWCCY 1 cut(s) 406
Psp6I CCWGG 4 cut(s) 400, 561, 709, 806
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 4 cut(s) 400, 561, 709, 806
PspN4I GGNNCC 8 cut(s) 311, 370, 399, 407, 408, 548, 636, 970
PspOMI GGGCCC 1 cut(s) 368
PspPI GGNCC 5 cut(s) 368, 369, 398, 406, 969
PspPPI RGGWCCY 1 cut(s) 406
PsuI RGATCY 2 cut(s) 634, 823
PvuII CAGCTG 2 cut(s) 851, 950
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 768, 1090
SacI GAGCTC 2 cut(s) 306, 417
SalI GTCGAC 1 cut(s) 889
SaqAI TTAA 3 cut(s) 261, 699, 758
SatI GCNGC 8 cut(s) 241, 293, 361, 364, 680, 723, 738, 849
Sau3AI GATC 4 cut(s) 352, 634, 823, 1056
Sau96I GGNCC 5 cut(s) 368, 369, 398, 406, 969
SchI GAGTC 3 cut(s) 22, 337, 886
ScrFI CCNGG 6 cut(s) 372, 373, 402, 563, 711, 808
SduI GDGCHC 4 cut(s) 306, 327, 372, 417
SfaNI GCATC 1 cut(s) 931
SfcI CTRYAG 1 cut(s) 1136
SinI GGWCC 2 cut(s) 406, 969
SmaI CCCGGG 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 768, 1090
SmlI CTYRAG 2 cut(s) 268, 345
SmoI CTYRAG 2 cut(s) 268, 345
Sse9I AATT 5 cut(s) 127, 262, 578, 690, 1023
SsiI CCGC 6 cut(s) 251, 360, 366, 638, 679, 935
SspMI CTAG 2 cut(s) 516, 746
SstI GAGCTC 2 cut(s) 306, 417
StyD4I CCNGG 6 cut(s) 370, 371, 400, 561, 709, 806
StyI CCWWGG 1 cut(s) 867
TaaI ACNGT 1 cut(s) 1089
TaiI ACGT 2 cut(s) 705, 1055
TaqI TCGA 5 cut(s) 165, 355, 766, 863, 890
TasI AATT 5 cut(s) 127, 262, 578, 690, 1023
TauI GCSGC 2 cut(s) 363, 682
TfiI GAWTC 4 cut(s) 19, 212, 751, 860
Tru1I TTAA 3 cut(s) 261, 699, 758
Tru9I TTAA 3 cut(s) 261, 699, 758
TscAI CASTG 1 cut(s) 191
TseI GCWGC 6 cut(s) 240, 292, 363, 722, 737, 848
TspDTI ATGAA 4 cut(s) 90, 440, 591, 1035
TspGWI ACGGA 1 cut(s) 744
TspMI CCCGGG 1 cut(s) 371
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 406, 969
XagI CCTNNNNNAGG 1 cut(s) 317
XmaI CCCGGG 1 cut(s) 371
XmiI GTMKAC 2 cut(s) 890, 931
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 516, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.