pycom07g18890

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002776
Physical Location & Seq
Forward (+)
21269743 .. 21273731
3989 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2520 bp
ATGGTCACCGATACTTATTCGATGATGTCCGACATGTCTATGAAATCGATGTTGAAAAGTGGCGAGTACAATGAAGATTTGAGCATTTTGATTCGCGAACAGAGGCGCCAGCACGAAGCAAGTGAGCGGGAGAAGGAACTGAATATATACAGAAGTGGGTCGGCGCCGCCTACCGTGGAAGGTTCGTTAAACGCGGTCGGTGGCTTGTTCGAGGCGTCCTCGCTGTCGGGTTTCACGAAAAATGACAGCAAAGGATTTGCAACCGAGGAGGAGCTTCGCGCCGATCCAGCTTATGTGAATTACTATTATTCCAATGTCAATCTCAATCCGCGGCTTCCGCCGCCGCTCCTTTCAAAGGAGGACTGGCGTTTTGCGCAGCGGTTGCAAGGCGGAGGTGGAGGTTCGGCGGTTGGAGATAGGAGGGTAGGTGGCAGGAGTGGTGGTGAAGGGTCACTTTTTTCTGTGCAGCCGGGGATTGGAGGGAATGAGGAGAATGGTGTGGCGGCGAGGAAGGGTGCTGCGGAGTGGGGCGGTGATGGGTTAATTGGGTTGCCGGGGTTGGGACTTGGGAGTAGGCAGAAGAGTATTGCAGAGATTATTCAGGATGATATACATAACACAAATGTCTCAAGGCACCCTTCTCGTCCAGCTAGCCAGAATGCGTTTGATGTTGATGCTTCAGATACCCAGTTTGCCCATTTACAGGGAATGTCAGCTGTTCAGAACGGGGGTTCATCCAGTTCTCACACATATGCTTCCGCTCTGGGTGCTTCATTGTTGAGAAGCAATACACCAGACCCTCAGCTTGTAGCTAGGGCTCCTAGTCCTCGAATTCCACCAGTTGGTGGAGGAAGGACTTCCTCCATGGATAAAAAAGTTGGCAATGGTCAAAACTCATTCAATGGTGCCTCGCCCAATGTGAACGAGTCTGTAGATCTGGCAGCTGCTTTATCTGGTATGAACCTGTCAACTAATGGTAGGATGGATGAGGAGAATCATGCACGATCACAAATTGATAATCACCATAACCGTTATGATATGCAAGGTGATCGGAATCATAATAAACAAAATTCGTACTTGAATAAGTCTGATTCTGGGAGTTTTCATCTGCATTCTTCTTCCCAATCTTCTAACAAGTCTTATCAGAATATGGCAAGAGGCAGTGGATTTGGTAGGGATCTGAATAGCCCTTCATATATGTCTGATGATCTAGTTGATATTAATAATCCTGCTGTATCTTCTGCGAACTCATACTTAAGAGGACCTGTGCCAACTCATCATGGCCGAGGAAGCTTACACTCCCACTATCAGAATGTAGACAATACCTCATTTCCGAACTACGGCTATTCTGGTAGTCCTTCGTCTCCATCCATGATGGGTAGTCCCCTTGGTAATGGTAGTTTACCACCCTTATTTGAAACTGCTGCTGCTGCATCTGCAATGGGTGGCTTGGACTCCGGAGCATTTGGAGGTATGTCTCTAGGACCAAATCTGTTGGCAGCAGCCGCTGAATTGCAGAACATTAGGGTTGGAAATCATGGTACTGGGAGTGCTCTTCAGGTTCCCATGATGGACCCATTGTATGTTCAGTACTTGAGATCGAATGAATATGCAGCTGCACAGCTTGCAGCTCTTAATGATCCCACAAAAGATAGGGAAGACATGGGTAGTATGTACATGGATTTACTTGGTTTGCAAAAAGCTTATTTGGGGCAATTCCTCTCACCCCAAAAATCACAATTTGGTGCTCCCTACGTTGGCAAGTCTGCTAGCTTGAATCACGGTTATTATGGAAATCCAGCATATGGGCATGGGATGTCTTATTCTGGAACACCATTGGGTGGTCCCCTTCTTCCAAACTCTCCTGTTGGACCTGGTAGTCCTGCCAGGCACGGTGAGCGGAACTTGCGCTTTTCTTCTGGGTTGAGGAACATGGGTGGAGGTCTCATGGGAGCTTGGCACTCCGAAACTGGTGGAAATTTGGATGAAAGTTTTGCGTCCTCTTTACTAGATGAGTTCAAAAGTAATAAGACTAGATGTTTTGAACTTTCAGAGATTGCAGGGCATGTTGTTGAGTTCAGTGCCGACCAGTATGGAAGTCGGTTTATTCAACAGAAACTTGAAACTGCCACAATTGAAGAAAAGAACATGGTTTTTGATGAAATCATGCCGCAAGCCCTATCTCTTATGACCGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTTGAACATGGAACTCCAGCACAAATAAGAGAACTTGCGGACCAGCTCACCGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAATTTGTTGAGCTGGACCAGCAGACTAAAATGGTAGCTGAACTGGATGGTCATGTTATGCGTTGTGTTCGTGATGATCCGATCCCAAAAAAAATGGCAACCATGTTGTTCAGAAGTGTATTGAATGTGTACCTGAAGATGCCATCCAGTTTGTTGTTTCAACTTTTTATGATCAAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

840

Amino Acids

90.31

Weight (kDa)

6.01

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 272 - 684 9.4e-151 Nucleic acid binding protein NABP
PUF PF00806 689 - 723 2e-11 Pumilio-family RNA binding repeat
PUF PF00806 726 - 758 6.8e-09 Pumilio-family RNA binding repeat
PUF PF00806 761 - 794 4.2e-09 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1878
Acc16I TGCGCA 1 cut(s) 375
AccB1I GGYRCC 4 cut(s) 105, 163, 633, 905
AccB7I CCANNNNNTGG 4 cut(s) 842, 845, 1805, 1841
AccBSI CCGCTC 4 cut(s) 127, 346, 761, 1900
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 4 cut(s) 96, 194, 279, 331
AccIII TCCGGA 1 cut(s) 1457
AclWI GGATC 6 cut(s) 278, 1185, 1634, 2317, 2408, 2413
AcoI YGGCCR 1 cut(s) 1282
AcsI RAATTY 4 cut(s) 831, 1069, 1978, 2339
AcuI CTGAAG 3 cut(s) 663, 1541, 2492
AcyI GRCGYC 3 cut(s) 106, 164, 215
AfaI GTAC 6 cut(s) 68, 1076, 1543, 1592, 1676, 2468
AflII CTTAAG 1 cut(s) 1255
AflIII ACRYGT 1 cut(s) 33
AjnI CCWGG 2 cut(s) 1873, 1886
Alw21I GWGCWC 2 cut(s) 1555, 1750
Alw26I GTCTC 4 cut(s) 631, 1368, 1482, 1949
AlwI GGATC 6 cut(s) 278, 1185, 1634, 2317, 2408, 2413
AlwNI CAGNNNCTG 1 cut(s) 1508
Aor13HI TCCGGA 1 cut(s) 1457
AoxI GGCC 1 cut(s) 1282
ApoI RAATTY 4 cut(s) 831, 1069, 1978, 2339
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AseI ATTAAT 1 cut(s) 1221
Asp700I GAANNNNTTC 1 cut(s) 856
AspLEI GCGC 5 cut(s) 108, 166, 281, 376, 1911
AspS9I GGNCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
AsuC2I CCSGG 3 cut(s) 471, 555, 2278
AsuHPI GGTGA 7 cut(s) 455, 545, 1013, 1058, 1716, 1907, 2266
AsuNHI GCTAGC 2 cut(s) 650, 1769
AvaII GGWCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
BanI GGYRCC 4 cut(s) 105, 163, 633, 905
BanII GRGCYC 1 cut(s) 820
BbsI GAAGAC 1 cut(s) 1665
Bbv12I GWGCWC 2 cut(s) 1555, 1750
BbvCI CCTCAGC 1 cut(s) 801
BccI CCATC 7 cut(s) 530, 976, 1369, 1375, 1564, 2378, 2488
BceAI ACGGC 1 cut(s) 1357
BcgI CGANNNNNNTGC 4 cut(s) 623, 657, 1031, 1065
BciT130I CCWGG 2 cut(s) 1875, 1888
BclI TGATCA 1 cut(s) 2508
BcnI CCSGG 3 cut(s) 471, 555, 2278
BcoDI GTCTC 4 cut(s) 631, 1368, 1482, 1949
BfaI CTAG 8 cut(s) 651, 813, 822, 1211, 1481, 1770, 2009, 2034
BfmI CTRYAG 2 cut(s) 930, 2334
BfoI RGCGCY 2 cut(s) 109, 167
BfrI CTTAAG 1 cut(s) 1255
BglII AGATCT 1 cut(s) 934
BmcAI AGTACT 1 cut(s) 1592
Bme1390I CCNGG 5 cut(s) 471, 555, 1875, 1888, 2278
Bme18I GGWCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
BmgT120I GGNCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
BmiI GGNNCC 8 cut(s) 107, 165, 635, 819, 907, 1563, 1575, 1846
BmrFI CCNGG 5 cut(s) 471, 555, 1875, 1888, 2278
BmrI ACTGGG 2 cut(s) 682, 1554
BmsI GCATC 3 cut(s) 664, 1442, 2466
BmtI GCTAGC 2 cut(s) 654, 1773
BmuI ACTGGG 2 cut(s) 682, 1554
BpiI GAAGAC 1 cut(s) 1665
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 1 cut(s) 2226
Bpu10I CCTNAGC 2 cut(s) 801, 2294
BpuEI CTTGAG 2 cut(s) 613, 1615
BpuMI CCSGG 3 cut(s) 471, 555, 2278
Bsa29I ATCGAT 1 cut(s) 47
BsaBI GATNNNNATC 1 cut(s) 1053
BsaHI GRCGYC 3 cut(s) 106, 164, 215
BsaI GGTCTC 1 cut(s) 1949
BsaJI CCNNGG 8 cut(s) 174, 264, 329, 470, 554, 864, 1285, 1387
BsaWI WCCGGW 1 cut(s) 1457
BsaXI ACNNNNNCTCC 4 cut(s) 412, 427, 442, 457
Bse1I ACTGG 9 cut(s) 368, 688, 738, 839, 1549, 1975, 2089, 2385, 2484
Bse3DI GCAATG 2 cut(s) 889, 1446
Bse8I GATNNNNATC 1 cut(s) 1053
BseAI TCCGGA 1 cut(s) 1457
BseBI CCWGG 2 cut(s) 1875, 1888
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 8 cut(s) 174, 264, 329, 470, 554, 864, 1285, 1387
BseGI GGATG 9 cut(s) 610, 734, 987, 991, 1367, 1821, 1990, 2389, 2480
BseJI GATNNNNATC 1 cut(s) 1053
BseMI GCAATG 2 cut(s) 889, 1446
BseMII CTCAG 1 cut(s) 815
BseNI ACTGG 9 cut(s) 368, 688, 738, 839, 1549, 1975, 2089, 2385, 2484
BseRI GAGGAG 4 cut(s) 281, 284, 503, 1004
BsgI GTGCAG 2 cut(s) 485, 1602
Bsh1236I CGCG 4 cut(s) 96, 194, 279, 331
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 1 cut(s) 1284
BshNI GGYRCC 4 cut(s) 105, 163, 633, 905
BshVI ATCGAT 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 1555, 1750
BsiSI CCGG 4 cut(s) 470, 554, 1458, 2277
BslFI GGGAC 3 cut(s) 576, 1368, 1830
BsmAI GTCTC 4 cut(s) 631, 1368, 1482, 1949
BsmBI CGTCTC 1 cut(s) 1368
BsmFI GGGAC 3 cut(s) 576, 1368, 1830
BsmI GAATGC 2 cut(s) 664, 1111
BsnI GGCC 1 cut(s) 1284
Bso31I GGTCTC 1 cut(s) 1949
Bsp1286I GDGCHC 3 cut(s) 820, 1555, 1750
Bsp13I TCCGGA 1 cut(s) 1457
Bsp1407I TGTACA 1 cut(s) 1674
Bsp19I CCATGG 1 cut(s) 864
Bsp68I TCGCGA 1 cut(s) 96
BspANI GGCC 1 cut(s) 1284
BspCNI CTCAG 1 cut(s) 814
BspDI ATCGAT 1 cut(s) 47
BspEI TCCGGA 1 cut(s) 1457
BspFNI CGCG 4 cut(s) 96, 194, 279, 331
BspLI GGNNCC 8 cut(s) 107, 165, 635, 819, 907, 1563, 1575, 1846
BspOI GCTAGC 2 cut(s) 654, 1773
BspPI GGATC 6 cut(s) 278, 1185, 1634, 2317, 2408, 2413
BspQI GCTCTTC 1 cut(s) 1560
BspT107I GGYRCC 4 cut(s) 105, 163, 633, 905
BspTI CTTAAG 1 cut(s) 1255
BspTNI GGTCTC 1 cut(s) 1949
BsrBI CCGCTC 4 cut(s) 127, 346, 761, 1900
BsrDI GCAATG 2 cut(s) 889, 1446
BsrGI TGTACA 1 cut(s) 1674
BsrI ACTGG 9 cut(s) 368, 688, 738, 839, 1549, 1975, 2089, 2385, 2484
BssECI CCNNGG 8 cut(s) 174, 264, 329, 470, 554, 864, 1285, 1387
BssNI GRCGYC 3 cut(s) 106, 164, 215
BssT1I CCWWGG 2 cut(s) 864, 1387
Bst2UI CCWGG 2 cut(s) 1875, 1888
Bst4CI ACNGT 4 cut(s) 175, 1031, 1784, 1895
Bst6I CTCTTC 2 cut(s) 575, 1560
BstACI GRCGYC 3 cut(s) 106, 164, 215
BstAFI CTTAAG 1 cut(s) 1255
BstAPI GCANNNNNTGC 2 cut(s) 382, 1625
BstAUI TGTACA 1 cut(s) 1674
BstC8I GCNNGC 5 cut(s) 110, 652, 1626, 1771, 2175
BstDEI CTNAG 2 cut(s) 801, 2294
BstDSI CCRYGG 3 cut(s) 174, 329, 864
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 9 cut(s) 610, 734, 987, 991, 1367, 1821, 1990, 2389, 2480
BstFNI CGCG 4 cut(s) 96, 194, 279, 331
BstH2I RGCGCY 2 cut(s) 109, 167
BstHHI GCGC 5 cut(s) 108, 166, 281, 376, 1911
BstMAI GTCTC 4 cut(s) 631, 1368, 1482, 1949
BstMCI CGRYCG 1 cut(s) 198
BstNI CCWGG 2 cut(s) 1875, 1888
BstNSI RCATGY 3 cut(s) 37, 2069, 2285
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 5 cut(s) 469, 553, 1873, 1886, 2276
BstSFI CTRYAG 2 cut(s) 930, 2334
BstUI CGCG 4 cut(s) 96, 194, 279, 331
BstV2I GAAGAC 1 cut(s) 1665
BstX2I RGATCY 2 cut(s) 934, 1177
BstYI RGATCY 2 cut(s) 934, 1177
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 1 cut(s) 1284
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 3 cut(s) 174, 329, 864
BtsCI GGATG 9 cut(s) 610, 734, 987, 991, 1367, 1821, 1990, 2389, 2480
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 2 cut(s) 1168, 2086
BtuMI TCGCGA 1 cut(s) 96
Cac8I GCNNGC 5 cut(s) 110, 652, 1626, 1771, 2175
CaiI CAGNNNCTG 1 cut(s) 1508
CfoI GCGC 5 cut(s) 108, 166, 281, 376, 1911
Cfr13I GGNCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
Cfr42I CCGCGG 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 47
CseI GACGC 2 cut(s) 204, 1986
CsiI ACCWGGT 1 cut(s) 1873
Csp6I GTAC 6 cut(s) 67, 1075, 1542, 1591, 1675, 2467
CviQI GTAC 6 cut(s) 67, 1075, 1542, 1591, 1675, 2467
DdeI CTNAG 2 cut(s) 801, 2294
DinI GGCGCC 2 cut(s) 107, 165
DrdI GACNNNNNNGTC 1 cut(s) 1878
DseDI GACNNNNNNGTC 1 cut(s) 1878
EaeI YGGCCR 1 cut(s) 1282
Eam1104I CTCTTC 2 cut(s) 575, 1560
EarI CTCTTC 2 cut(s) 575, 1560
EciI GGCGGA 2 cut(s) 327, 405
Eco130I CCWWGG 2 cut(s) 864, 1387
Eco24I GRGCYC 1 cut(s) 820
Eco31I GGTCTC 1 cut(s) 1949
Eco47I GGWCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
Eco57I CTGAAG 3 cut(s) 663, 1541, 2492
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 1262
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 831
EcoRII CCWGG 2 cut(s) 1873, 1886
EcoT14I CCWWGG 2 cut(s) 864, 1387
EcoT38I GRGCYC 1 cut(s) 820
EgeI GGCGCC 2 cut(s) 107, 165
EheI GGCGCC 2 cut(s) 107, 165
ErhI CCWWGG 2 cut(s) 864, 1387
Esp3I CGTCTC 1 cut(s) 1368
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 622, 654
FaqI GGGAC 3 cut(s) 576, 1368, 1830
FauI CCCGC 1 cut(s) 120
FauNDI CATATG 2 cut(s) 751, 1804
FbaI TGATCA 1 cut(s) 2508
FblI GTMKAC 1 cut(s) 1317
FokI GGATG 9 cut(s) 617, 721, 994, 998, 1354, 1828, 1997, 2396, 2467
FriOI GRGCYC 1 cut(s) 820
FspBI CTAG 8 cut(s) 651, 813, 822, 1211, 1481, 1770, 2009, 2034
FspI TGCGCA 1 cut(s) 375
GlaI GCGC 5 cut(s) 107, 165, 280, 375, 1910
GsuI CTGGAG 1 cut(s) 2226
HaeII RGCGCY 2 cut(s) 109, 167
HaeIII GGCC 1 cut(s) 1284
HapII CCGG 4 cut(s) 470, 554, 1458, 2277
HgaI GACGC 2 cut(s) 204, 1986
HhaI GCGC 5 cut(s) 108, 166, 281, 376, 1911
Hin1I GRCGYC 3 cut(s) 106, 164, 215
Hin6I GCGC 5 cut(s) 106, 164, 279, 374, 1909
HinP1I GCGC 5 cut(s) 106, 164, 279, 374, 1909
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HindIII AAGCTT 2 cut(s) 1291, 1701
HinfI GANTC 8 cut(s) 91, 926, 994, 1054, 1091, 1454, 1777, 2215
HpaII CCGG 4 cut(s) 470, 554, 1458, 2277
HphI GGTGA 7 cut(s) 455, 545, 1013, 1058, 1716, 1907, 2266
Hpy166II GTNNAC 5 cut(s) 922, 969, 1318, 1403, 2467
Hpy188III TCNNGA 8 cut(s) 95, 235, 602, 1458, 1827, 2326, 2408, 2517
Hpy8I GTNNAC 5 cut(s) 922, 969, 1318, 1403, 2467
HpyCH4III ACNGT 4 cut(s) 175, 1031, 1784, 1895
HpyCH4IV ACGT 1 cut(s) 1755
HpyF3I CTNAG 2 cut(s) 801, 2294
HpySE526I ACGT 1 cut(s) 1755
Hsp92I GRCGYC 3 cut(s) 106, 164, 215
HspAI GCGC 5 cut(s) 106, 164, 279, 374, 1909
KasI GGCGCC 2 cut(s) 105, 163
Kpn2I TCCGGA 1 cut(s) 1457
Ksp22I TGATCA 1 cut(s) 2508
KspI CCGCGG 1 cut(s) 332
LguI GCTCTTC 1 cut(s) 1560
LmnI GCTCC 6 cut(s) 271, 351, 823, 1460, 1753, 1952
LweI GCATC 3 cut(s) 664, 1442, 2466
MabI ACCWGGT 1 cut(s) 1873
MaeI CTAG 8 cut(s) 651, 813, 822, 1211, 1481, 1770, 2009, 2034
MaeII ACGT 1 cut(s) 1755
MaeIII GTNAC 2 cut(s) 4, 450
MbiI CCGCTC 4 cut(s) 127, 346, 761, 1900
MfeI CAATTG 1 cut(s) 2133
MflI RGATCY 2 cut(s) 934, 1177
MhlI GDGCHC 3 cut(s) 820, 1555, 1750
Mly113I GGCGCC 2 cut(s) 106, 164
MlyI GAGTC 2 cut(s) 935, 1448
MmeI TCCRAC 4 cut(s) 54, 391, 1510, 1849
MroI TCCGGA 1 cut(s) 1457
MroXI GAANNNNTTC 1 cut(s) 856
MseI TTAA 5 cut(s) 188, 542, 1221, 1256, 1635
MslI CAYNNNNRTG 2 cut(s) 38, 750
MspA1I CMGCKG 6 cut(s) 331, 379, 716, 944, 1508, 1616
MspCI CTTAAG 1 cut(s) 1255
MspI CCGG 4 cut(s) 470, 554, 1458, 2277
MspR9I CCNGG 5 cut(s) 471, 555, 1875, 1888, 2278
MunI CAATTG 1 cut(s) 2133
Mva1269I GAATGC 2 cut(s) 664, 1111
MvaI CCWGG 2 cut(s) 1875, 1888
MvnI CGCG 4 cut(s) 96, 194, 279, 331
NarI GGCGCC 2 cut(s) 106, 164
NciI CCSGG 3 cut(s) 471, 555, 2278
NcoI CCATGG 1 cut(s) 864
NdeI CATATG 2 cut(s) 751, 1804
NheI GCTAGC 2 cut(s) 650, 1769
NlaIV GGNNCC 8 cut(s) 107, 165, 635, 819, 907, 1563, 1575, 1846
NmeAIII GCCGAG 1 cut(s) 1310
NmuCI GTSAC 2 cut(s) 4, 450
NruI TCGCGA 1 cut(s) 96
NsbI TGCGCA 1 cut(s) 375
NspI RCATGY 3 cut(s) 37, 2069, 2285
PciI ACATGT 1 cut(s) 33
PciSI GCTCTTC 1 cut(s) 1560
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PctI GAATGC 2 cut(s) 664, 1111
PdmI GAANNNNTTC 1 cut(s) 856
PfeI GAWTC 6 cut(s) 91, 994, 1054, 1091, 1777, 2215
PflMI CCANNNNNTGG 4 cut(s) 842, 845, 1805, 1841
PleI GAGTC 2 cut(s) 934, 1448
PluTI GGCGCC 2 cut(s) 109, 167
PpsI GAGTC 2 cut(s) 934, 1448
PpuMI RGGWCCY 1 cut(s) 1262
PscI ACATGT 1 cut(s) 33
PshBI ATTAAT 1 cut(s) 1221
Psp5II RGGWCCY 1 cut(s) 1262
Psp6I CCWGG 2 cut(s) 1873, 1886
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 1873, 1886
PspN4I GGNNCC 8 cut(s) 107, 165, 635, 819, 907, 1563, 1575, 1846
PspPI GGNCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
PspPPI RGGWCCY 1 cut(s) 1262
PstNI CAGNNNCTG 1 cut(s) 1508
PsuI RGATCY 2 cut(s) 934, 1177
PvuII CAGCTG 3 cut(s) 716, 944, 1616
RruI TCGCGA 1 cut(s) 96
RsaI GTAC 6 cut(s) 68, 1076, 1543, 1592, 1676, 2468
RsaNI GTAC 6 cut(s) 67, 1075, 1542, 1591, 1675, 2467
RseI CAYNNNNRTG 2 cut(s) 38, 750
SacII CCGCGG 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 1560
SaqAI TTAA 5 cut(s) 188, 542, 1221, 1256, 1635
Sau96I GGNCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
ScaI AGTACT 1 cut(s) 1592
SchI GAGTC 2 cut(s) 935, 1448
ScrFI CCNGG 5 cut(s) 471, 555, 1875, 1888, 2278
SduI GDGCHC 3 cut(s) 820, 1555, 1750
SexAI ACCWGGT 1 cut(s) 1873
SfaNI GCATC 3 cut(s) 664, 1442, 2466
SfcI CTRYAG 2 cut(s) 930, 2334
SfoI GGCGCC 2 cut(s) 107, 165
Sfr303I CCGCGG 1 cut(s) 332
SgrBI CCGCGG 1 cut(s) 332
SinI GGWCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
SmiMI CAYNNNNRTG 2 cut(s) 38, 750
SmlI CTYRAG 3 cut(s) 628, 1255, 1594
SmoI CTYRAG 3 cut(s) 628, 1255, 1594
SspDI GGCGCC 2 cut(s) 105, 163
SspMI CTAG 8 cut(s) 651, 813, 822, 1211, 1481, 1770, 2009, 2034
StyD4I CCNGG 5 cut(s) 469, 553, 1873, 1886, 2276
StyI CCWWGG 2 cut(s) 864, 1387
TaaI ACNGT 4 cut(s) 175, 1031, 1784, 1895
TaiI ACGT 1 cut(s) 1758
TaqI TCGA 6 cut(s) 20, 47, 210, 829, 1601, 2317
TaqII GACCGA 1 cut(s) 2207
TatI WGTACW 3 cut(s) 66, 1590, 1674
TauI GCSGC 7 cut(s) 169, 334, 343, 346, 506, 1508, 2173
TfiI GAWTC 6 cut(s) 91, 994, 1054, 1091, 1777, 2215
Tru1I TTAA 5 cut(s) 188, 542, 1221, 1256, 1635
Tru9I TTAA 5 cut(s) 188, 542, 1221, 1256, 1635
TscAI CASTG 2 cut(s) 1168, 2086
TseFI GTSAC 2 cut(s) 4, 450
Tsp45I GTSAC 2 cut(s) 4, 450
TspRI CASTG 2 cut(s) 1168, 2086
Van91I CCANNNNNTGG 4 cut(s) 842, 845, 1805, 1841
Vha464I CTTAAG 1 cut(s) 1255
VpaK11BI GGWCC 7 cut(s) 1262, 1484, 1573, 1844, 1871, 2266, 2353
VspI ATTAAT 1 cut(s) 1221
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 4 cut(s) 831, 1069, 1978, 2339
XceI RCATGY 3 cut(s) 37, 2069, 2285
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 1 cut(s) 856
XspI CTAG 8 cut(s) 651, 813, 822, 1211, 1481, 1770, 2009, 2034
ZrmI AGTACT 1 cut(s) 1592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.