pycom07g27170

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004548
Physical Location & Seq
Reverse (-)
26942117 .. 26942991
875 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGCATCATCTCCTAGTGTCTGGTTTACATTACTGCTTACTCATTAAACAACGTCATCAACAATTTAACAAGCTACATATTTATGAACATCGTCTGCTAGAGAATGATTCATTCCCACCACCGCCTCCACAAGCAGAGACTCCTTCATGGCGGCCTTCACCTTGGTTGGTTCCGTTACCGGTCCACTGCATAGCTGAATCCGTCAAGAACCAACTCCCGGTGCTGCAATACCAAGAAACTCTCATGAAAACACGAAGGCAAGCAGAAGCAGAAGAACAGGACTGCCACGTGAACATGTGCATAGTGTGCATGGATAGCATGGAGGAAGGCCAAGCGGTGAGAGAGCCATGCAATTGTTCGCATGGTTTTCATAAGGAGTGCCTAGATGTCTGGATTGATCAAGGTCAGCTAACATGTCCTCTCTGTAGATTGGAGCTATTGCCTAAAACTTGTAGTACTTCCCCAAACTCAAAAGATAAGGATCCATGGAGGAAGGAGAGGATGGTTTACTTGTTTGGTGATGATTACTTCATGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

180

Amino Acids

20.94

Weight (kDa)

6.04

Isoelectric Point (pI)

68.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 98 - 143 7.1e-11 Ring finger domain
zf-rbx1 PF12678 98 - 143 1.4e-06 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 99 - 142 9.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 100 - 128 1.9e-07 RING-like zinc finger
zf-C3HC4 PF00097 100 - 142 2.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 122, 151, 335
AclWI GGATC 2 cut(s) 476, 489
AcvI CACGTG 1 cut(s) 289
AfaI GTAC 1 cut(s) 457
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 2 cut(s) 294, 413
AgeI ACCGGT 1 cut(s) 178
AluBI AGCT 5 cut(s) 73, 194, 409, 436, 537
AluI AGCT 5 cut(s) 73, 194, 409, 436, 537
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 2 cut(s) 476, 489
AoxI GGCC 2 cut(s) 152, 328
ApeKI GCWGC 1 cut(s) 223
AsiGI ACCGGT 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 3 cut(s) 150, 349, 530
AvaII GGWCC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 481
BbrPI CACGTG 1 cut(s) 289
BbvI GCAGC 1 cut(s) 210
BccI CCATC 1 cut(s) 496
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 4 cut(s) 14, 98, 383, 538
BfmI CTRYAG 1 cut(s) 424
BisI GCNGC 2 cut(s) 152, 224
BlsI GCNGC 2 cut(s) 153, 225
BmcAI AGTACT 1 cut(s) 457
Bme1390I CCNGG 1 cut(s) 218
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 171, 483
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 289
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 161, 485
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 161, 485
BseGI GGATG 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 210
BshFI GGCC 2 cut(s) 154, 330
BshTI ACCGGT 1 cut(s) 178
BsiSI CCGG 2 cut(s) 179, 218
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 2 cut(s) 154, 330
Bsp143I GATC 2 cut(s) 397, 481
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 3 cut(s) 122, 151, 335
BspANI GGCC 2 cut(s) 154, 330
BspHI TCATGA 2 cut(s) 243, 531
BspLI GGNNCC 2 cut(s) 171, 483
BspPI GGATC 2 cut(s) 476, 489
BsrFI RCCGGY 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 161, 485
BssMI GATC 2 cut(s) 397, 481
BssT1I CCWWGG 2 cut(s) 161, 485
BstAPI GCANNNNNTGC 1 cut(s) 306
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 1 cut(s) 507
BstKTI GATC 2 cut(s) 400, 484
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 397, 481
BstMWI GCNNNNNNNGC 2 cut(s) 306, 315
BstNSI RCATGY 2 cut(s) 298, 417
BstSCI CCNGG 1 cut(s) 216
BstSFI CTRYAG 1 cut(s) 424
BstV1I GCAGC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BsuRI GGCC 2 cut(s) 154, 330
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 261
CciI TCATGA 2 cut(s) 243, 531
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 456
CspAI ACCGGT 1 cut(s) 178
CviJI RGCY 8 cut(s) 73, 154, 194, 330, 346, 409, 436, 537
CviKI_1 RGCY 8 cut(s) 73, 154, 194, 330, 346, 409, 436, 537
CviQI GTAC 1 cut(s) 456
DpnI GATC 2 cut(s) 399, 483
DpnII GATC 2 cut(s) 397, 481
Eco130I CCWWGG 2 cut(s) 161, 485
Eco47I GGWCC 1 cut(s) 181
Eco72I CACGTG 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 161, 485
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 161, 485
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 152, 224
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 2 cut(s) 152, 224
FspBI CTAG 4 cut(s) 14, 98, 383, 538
GluI GCNGC 2 cut(s) 152, 224
HaeIII GGCC 2 cut(s) 154, 330
HapII CCGG 2 cut(s) 179, 218
HinfI GANTC 3 cut(s) 107, 139, 197
HpaII CCGG 2 cut(s) 179, 218
HphI GGTGA 3 cut(s) 150, 349, 530
Hpy166II GTNNAC 4 cut(s) 26, 184, 292, 508
Hpy188III TCNNGA 4 cut(s) 205, 244, 391, 532
Hpy8I GTNNAC 4 cut(s) 26, 184, 292, 508
HpyAV CCTTC 5 cut(s) 153, 165, 249, 320, 487
HpyCH4IV ACGT 2 cut(s) 52, 288
HpyCH4V TGCA 6 cut(s) 4, 189, 226, 300, 309, 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 315
HpySE526I ACGT 2 cut(s) 52, 288
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 2 cut(s) 397, 481
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 5 cut(s) 6, 192, 231, 263, 376
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 1 cut(s) 13
MaeI CTAG 4 cut(s) 14, 98, 383, 538
MaeII ACGT 2 cut(s) 52, 288
MaeIII GTNAC 1 cut(s) 174
MalI GATC 2 cut(s) 399, 483
MboI GATC 2 cut(s) 397, 481
MboII GAAGA 1 cut(s) 284
MfeI CAATTG 1 cut(s) 352
MflI RGATCY 1 cut(s) 481
MluCI AATT 2 cut(s) 62, 352
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 5 cut(s) 135, 316, 429, 483, 492
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 45, 66
MslI CAYNNNNRTG 1 cut(s) 81
MspI CCGG 2 cut(s) 179, 218
MspR9I CCNGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 306, 315
NciI CCSGG 1 cut(s) 218
NcoI CCATGG 1 cut(s) 485
NdeII GATC 2 cut(s) 397, 481
NlaIV GGNNCC 2 cut(s) 171, 483
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 298, 417
PagI TCATGA 2 cut(s) 243, 531
PciI ACATGT 2 cut(s) 294, 413
PfeI GAWTC 2 cut(s) 107, 197
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 2 cut(s) 153, 225
PleI GAGTC 1 cut(s) 133
PmaCI CACGTG 1 cut(s) 289
PmlI CACGTG 1 cut(s) 289
PpsI GAGTC 1 cut(s) 133
Ppu21I YACGTR 1 cut(s) 289
PscI ACATGT 2 cut(s) 294, 413
PspCI CACGTG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 171, 483
PspPI GGNCC 1 cut(s) 181
PsuI RGATCY 1 cut(s) 481
RsaI GTAC 1 cut(s) 457
RsaNI GTAC 1 cut(s) 456
RseI CAYNNNNRTG 1 cut(s) 81
SaqAI TTAA 2 cut(s) 45, 66
SatI GCNGC 2 cut(s) 152, 224
Sau3AI GATC 2 cut(s) 397, 481
Sau96I GGNCC 1 cut(s) 181
ScaI AGTACT 1 cut(s) 457
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 218
SetI ASST 9 cut(s) 55, 75, 163, 196, 291, 406, 411, 438, 539
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 81
Sse9I AATT 2 cut(s) 62, 352
SsiI CCGC 3 cut(s) 122, 151, 335
SspMI CTAG 4 cut(s) 14, 98, 383, 538
StyD4I CCNGG 1 cut(s) 216
StyI CCWWGG 2 cut(s) 161, 485
TaiI ACGT 2 cut(s) 55, 291
TasI AATT 2 cut(s) 62, 352
TatI WGTACW 1 cut(s) 455
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 2 cut(s) 107, 197
Tru1I TTAA 2 cut(s) 45, 66
Tru9I TTAA 2 cut(s) 45, 66
TscAI CASTG 1 cut(s) 191
TseI GCWGC 1 cut(s) 223
TspDTI ATGAA 6 cut(s) 99, 99, 135, 260, 359, 520
TspGWI ACGGA 2 cut(s) 162, 190
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 181
XceI RCATGY 2 cut(s) 298, 417
XspI CTAG 4 cut(s) 14, 98, 383, 538
ZrmI AGTACT 1 cut(s) 457
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.