pycom10g24070

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001932
Physical Location & Seq
Reverse (-)
25964664 .. 25965601
938 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGACCCCGTCCAGATCTCAGCTGCATTGTCATTTTAGCACCAGGAGATTAGAAGGTATGGTTTTTCAGAAGGAGTACTTAGATGTTGTGCTGGTCCCGTTGGGGCTTTTGATCATGCTAATCTACCACCTCTTCCTCCTCTACAAATACCTCAATCACCCTCTCTCCACCGCTATTGGGTCCGAAAACATTGACAGGAAAATTTGGGTTGAAAAAATTTTGCAGGGTGACGATGTTGAAGTTGCAACGAAAGTTAATACAGCTCTTGATGTGATTTCCTCCAATACAAGTGCAACTACAACCTTAGCATCAATCTCTCTAACTCTCTGCGCTCTCATCGGAGCTTGGATGGCAAACTCAGCCAATAACTTCTTGCCAAGCCAAATAATATATGGCAACACAAACCCAGTGACTGTTTCTATCAAGTACATTTGCATCTTGACATGTTTTCTCCTTGCTTTTTCATGCTTTGTTCAATCAGCTCGGCACTTTGTCCACTCAAACTACCTTTTAGCCACCCCGGGAGCTACCGCGAAAAGCGACGTGAAAAAAGCCAAGGGAGCTGTTCAAAGGGGAAGTGAGTTTTGGTCACTTGGGCTTAGAGCTCTCTACTTTGCTCTCAATTTTCTGCTGTGGTTTTTTGGACCTATTCCCATGTTAGTTTCCTCCGTCGCAACCGTTGTGATCCTATCTTGCCACGACTATGAGAAGTCCGGCGGTGATCACAAGCGGCCGCCATCATCGGCCGTGGATACAAATGAACGTTTATTATTGGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

261

Amino Acids

28.75

Weight (kDa)

8.7

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 30 - 235 2.5e-57 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 533
AciI CCGC 5 cut(s) 171, 531, 717, 730, 734
AclI AACGTT 1 cut(s) 763
AclWI GGATC 1 cut(s) 679
AcoI YGGCCR 2 cut(s) 731, 744
AcsI RAATTY 2 cut(s) 201, 216
AfaI GTAC 2 cut(s) 77, 428
AfiI CCNNNNNNNGG 1 cut(s) 177
AflIII ACRYGT 1 cut(s) 443
AgsI TTSAA 4 cut(s) 212, 239, 476, 569
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AluBI AGCT 7 cut(s) 22, 263, 344, 482, 527, 563, 605
AluI AGCT 7 cut(s) 22, 263, 344, 482, 527, 563, 605
Alw21I GWGCWC 1 cut(s) 607
AlwI GGATC 1 cut(s) 679
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 520
AoxI GGCC 2 cut(s) 731, 744
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 2 cut(s) 201, 216
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 94, 180, 644
AsuC2I CCSGG 2 cut(s) 521, 522
AsuHPI GGTGA 3 cut(s) 149, 239, 731
AvaI CYCGRG 1 cut(s) 520
AvaII GGWCC 3 cut(s) 94, 180, 644
BanII GRGCYC 1 cut(s) 607
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 1 cut(s) 9
BccI CCATC 2 cut(s) 343, 745
BceAI ACGGC 1 cut(s) 731
BciT130I CCWGG 1 cut(s) 43
BciVI GTATCC 1 cut(s) 745
BclI TGATCA 2 cut(s) 111, 721
BcnI CCSGG 2 cut(s) 521, 522
BfuI GTATCC 1 cut(s) 745
BglII AGATCT 1 cut(s) 14
BisI GCNGC 3 cut(s) 23, 731, 734
BlsI GCNGC 3 cut(s) 24, 732, 735
BmcAI AGTACT 1 cut(s) 77
Bme1390I CCNGG 3 cut(s) 43, 521, 522
Bme18I GGWCC 3 cut(s) 94, 180, 644
BmeT110I CYCGRG 1 cut(s) 520
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 94, 180, 644
BmiI GGNNCC 2 cut(s) 96, 181
BmrFI CCNGG 3 cut(s) 43, 521, 522
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 2 cut(s) 317, 444
BmuI ACTGGG 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 521, 522
BsaJI CCNNGG 4 cut(s) 519, 520, 555, 747
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 407
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 4 cut(s) 519, 520, 555, 747
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMII CTCAG 2 cut(s) 32, 372
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 1 cut(s) 128
BseX3I CGGCCG 2 cut(s) 731, 744
BseXI GCAGC 1 cut(s) 9
Bsh1236I CGCG 1 cut(s) 533
Bsh1285I CGRYCG 2 cut(s) 734, 747
BshFI GGCC 2 cut(s) 733, 746
BsiEI CGRYCG 2 cut(s) 734, 747
BsiHKAI GWGCWC 1 cut(s) 607
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 2 cut(s) 521, 714
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 2 cut(s) 733, 746
BsoBI CYCGRG 1 cut(s) 520
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 4 cut(s) 14, 111, 684, 721
BspACI CCGC 5 cut(s) 171, 531, 717, 730, 734
BspANI GGCC 2 cut(s) 733, 746
BspCNI CTCAG 2 cut(s) 31, 371
BspFNI CGCG 1 cut(s) 533
BspLI GGNNCC 2 cut(s) 96, 181
BspPI GGATC 1 cut(s) 679
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 519, 520, 555, 747
BssMI GATC 4 cut(s) 14, 111, 684, 721
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 2 cut(s) 415, 679
Bst6I CTCTTC 1 cut(s) 137
BstDEI CTNAG 5 cut(s) 18, 79, 304, 358, 599
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 1 cut(s) 533
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 4 cut(s) 17, 114, 687, 724
BstMBI GATC 4 cut(s) 14, 111, 684, 721
BstMCI CGRYCG 2 cut(s) 734, 747
BstMWI GCNNNNNNNGC 3 cut(s) 350, 359, 560
BstNI CCWGG 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 447
BstSCI CCNGG 3 cut(s) 41, 519, 520
BstUI CGCG 1 cut(s) 533
BstV1I GCAGC 1 cut(s) 9
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BstZI CGGCCG 2 cut(s) 731, 744
BsuI GTATCC 1 cut(s) 745
BsuRI GGCC 2 cut(s) 733, 746
BtgI CCRYGG 1 cut(s) 747
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 414
CaiI CAGNNNCTG 1 cut(s) 413
CciNI GCGGCCGC 1 cut(s) 731
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 3 cut(s) 94, 180, 644
Cfr9I CCCGGG 1 cut(s) 520
Csp6I GTAC 2 cut(s) 76, 427
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 4 cut(s) 115, 444, 465, 655
CviQI GTAC 2 cut(s) 76, 427
DdeI CTNAG 5 cut(s) 18, 79, 304, 358, 599
DpnI GATC 4 cut(s) 16, 113, 686, 723
DpnII GATC 4 cut(s) 14, 111, 684, 721
EaeI YGGCCR 2 cut(s) 731, 744
EagI CGGCCG 2 cut(s) 731, 744
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Ecl136II GAGCTC 1 cut(s) 605
EclXI CGGCCG 2 cut(s) 731, 744
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 607
Eco47I GGWCC 3 cut(s) 94, 180, 644
Eco52I CGGCCG 2 cut(s) 731, 744
Eco53kI GAGCTC 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 520
EcoICRI GAGCTC 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 4 cut(s) 118, 447, 468, 658
FaiI YATR 8 cut(s) 59, 116, 391, 393, 445, 466, 656, 705
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 1 cut(s) 80
FatI CATG 4 cut(s) 114, 443, 464, 654
FbaI TGATCA 2 cut(s) 111, 721
Fnu4HI GCNGC 3 cut(s) 23, 731, 734
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 1 cut(s) 607
Fsp4HI GCNGC 3 cut(s) 23, 731, 734
GlaI GCGC 1 cut(s) 331
GluI GCNGC 3 cut(s) 23, 731, 734
HaeIII GGCC 2 cut(s) 733, 746
HapII CCGG 2 cut(s) 521, 714
HhaI GCGC 1 cut(s) 332
Hin1II CATG 4 cut(s) 118, 447, 468, 658
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HpaII CCGG 2 cut(s) 521, 714
HphI GGTGA 3 cut(s) 149, 239, 731
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 3 cut(s) 69, 184, 341
Hpy188III TCNNGA 4 cut(s) 12, 266, 439, 780
Hpy8I GTNNAC 1 cut(s) 496
Hpy99I CGWCG 2 cut(s) 545, 674
HpyAV CCTTC 2 cut(s) 47, 64
HpyCH4III ACNGT 2 cut(s) 415, 679
HpyCH4IV ACGT 2 cut(s) 543, 763
HpyCH4V TGCA 5 cut(s) 25, 223, 245, 293, 435
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 359, 560
HpyF3I CTNAG 5 cut(s) 18, 79, 304, 358, 599
HpySE526I ACGT 2 cut(s) 543, 763
Hsp92II CATG 4 cut(s) 118, 447, 468, 658
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 2 cut(s) 111, 721
Kzo9I GATC 4 cut(s) 14, 111, 684, 721
LmnI GCTCC 3 cut(s) 341, 524, 560
LpnPI CCDG 9 cut(s) 25, 28, 55, 77, 181, 209, 420, 534, 727
Lsp1109I GCAGC 1 cut(s) 9
LweI GCATC 2 cut(s) 317, 444
MaeII ACGT 2 cut(s) 543, 763
MaeIII GTNAC 3 cut(s) 227, 409, 588
MalI GATC 4 cut(s) 16, 113, 686, 723
MboI GATC 4 cut(s) 14, 111, 684, 721
MboII GAAGA 1 cut(s) 124
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 3 cut(s) 201, 216, 622
MnlI CCTC 7 cut(s) 140, 146, 149, 161, 171, 289, 676
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 702
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 2 cut(s) 521, 714
MspR9I CCNGG 3 cut(s) 43, 521, 522
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 3 cut(s) 350, 359, 560
NciI CCSGG 2 cut(s) 521, 522
NdeII GATC 4 cut(s) 14, 111, 684, 721
NlaIII CATG 4 cut(s) 118, 447, 468, 658
NlaIV GGNNCC 2 cut(s) 96, 181
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 3 cut(s) 227, 409, 588
NotI GCGGCCGC 1 cut(s) 731
NspI RCATGY 1 cut(s) 447
PciI ACATGT 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 3 cut(s) 24, 732, 735
PscI ACATGT 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 763
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 96, 181
PspPI GGNCC 3 cut(s) 94, 180, 644
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 1 cut(s) 14
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 2 cut(s) 77, 428
RsaNI GTAC 2 cut(s) 76, 427
RseI CAYNNNNRTG 1 cut(s) 702
SacI GAGCTC 1 cut(s) 607
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 3 cut(s) 23, 731, 734
Sau3AI GATC 4 cut(s) 14, 111, 684, 721
Sau96I GGNCC 3 cut(s) 94, 180, 644
ScaI AGTACT 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 43, 521, 522
SduI GDGCHC 1 cut(s) 607
SfaNI GCATC 2 cut(s) 317, 444
SinI GGWCC 3 cut(s) 94, 180, 644
SmaI CCCGGG 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 702
Sse9I AATT 3 cut(s) 201, 216, 622
SsiI CCGC 5 cut(s) 171, 531, 717, 730, 734
SstI GAGCTC 1 cut(s) 607
StyD4I CCNGG 3 cut(s) 41, 519, 520
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 2 cut(s) 415, 679
TaiI ACGT 2 cut(s) 546, 766
TasI AATT 3 cut(s) 201, 216, 622
TatI WGTACW 2 cut(s) 75, 426
TauI GCSGC 2 cut(s) 733, 736
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 3 cut(s) 227, 409, 588
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 3 cut(s) 227, 409, 588
TspDTI ATGAA 2 cut(s) 453, 774
TspGWI ACGGA 1 cut(s) 658
TspMI CCCGGG 1 cut(s) 520
TspRI CASTG 1 cut(s) 414
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 3 cut(s) 94, 180, 644
XapI RAATTY 2 cut(s) 201, 216
XceI RCATGY 1 cut(s) 447
XcmI CCANNNNNNNNNTGG 1 cut(s) 389
XmaI CCCGGG 1 cut(s) 520
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.