pycom10g26260

Histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001504
Physical Location & Seq
Reverse (-)
27636511 .. 27637187
677 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26260.2

Sequence Viewer

Length: 528 bp
ATGGCCACAGATTGCAATCCGCGTCACTCCCTCTCATTGAAACTCCCCAAAACACTCATCCCGCTATTTCATAACTTCATTCACATCGCGAATAATTTGAGATTCTCTCTCTCTCTCTTCTCCGAATCCATGGCCGGAAGAGGAAAATCAATCGGCTCCGGCACCGCCAAGAAGGCCACGTCTCGGAGCAGCAAGGCCGGTCTCCAATTCCCCGTCGGCCGTATCGCCCGGTTCTTGAAAGCTGGCAAGTACGCCGAGCGTGTTGGCGCCGGCGCCCCTGTCTACCTCGCCGCCGTCCTTGAATACCTCGCCGCAGAGGTTTTGGAATTGGCCGGAAACGCCGCACGTGACAACAAGAAGACGAGGATTGTGCCGAGGCACATCCAGCTGGCCGTCAGGAACGACGAGGAGCTCAGCAAGCTGCTCGGCGCCGTCACCATCGCCAACGGCGGCGTTATGCCCAACATCCACAACATGCTCCTCCCCAAGAAAACCGGCACCGGCAAATCTGGCCCCGCCGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

176

Amino Acids

18.69

Weight (kDa)

10.25

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 161, 266, 272, 428, 497
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 22, 89
AciI CCGC 8 cut(s) 20, 62, 165, 291, 312, 342, 450, 516
AcoI YGGCCR 5 cut(s) 3, 132, 217, 330, 390
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 3 cut(s) 267, 273, 429
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 3 cut(s) 40, 238, 302
AjiI CACGTC 1 cut(s) 180
AluBI AGCT 4 cut(s) 242, 388, 412, 421
AluI AGCT 4 cut(s) 242, 388, 412, 421
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 186, 206
AoxI GGCC 8 cut(s) 3, 132, 174, 195, 217, 330, 390, 511
ApeKI GCWGC 2 cut(s) 189, 421
AspLEI GCGC 3 cut(s) 269, 275, 431
AspS9I GGNCC 1 cut(s) 512
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 1 cut(s) 427
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 5 cut(s) 161, 266, 272, 428, 497
BanII GRGCYC 1 cut(s) 414
BbrPI CACGTG 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 2 cut(s) 201, 408
BccI CCATC 1 cut(s) 446
BceAI ACGGC 5 cut(s) 204, 278, 377, 416, 463
BcgI CGANNNNNNTGC 4 cut(s) 352, 386, 406, 440
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 186, 206
BfaI CTAG 1 cut(s) 526
BfoI RGCGCY 3 cut(s) 270, 276, 432
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 6 cut(s) 190, 291, 312, 342, 422, 451
BlpI GCTNAGC 1 cut(s) 413
BlsI GCNGC 6 cut(s) 191, 292, 313, 343, 423, 452
Bme1390I CCNGG 1 cut(s) 229
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 7 cut(s) 157, 163, 268, 274, 430, 499, 514
BmrFI CCNGG 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 91, 123
Bpu1102I GCTNAGC 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 229
BsaAI YACGTR 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 3 cut(s) 267, 273, 429
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 129, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 4 cut(s) 197, 269, 494, 500
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 129, 374
BseGI GGATG 3 cut(s) 57, 381, 465
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 427
BseRI GAGGAG 2 cut(s) 422, 470
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 201, 408
Bsh1236I CGCG 2 cut(s) 22, 89
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 8 cut(s) 5, 134, 176, 197, 219, 332, 392, 513
BshNI GGYRCC 5 cut(s) 161, 266, 272, 428, 497
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 8 cut(s) 135, 159, 198, 229, 270, 333, 495, 501
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 2 cut(s) 186, 206
BsmBI CGTCTC 1 cut(s) 186
BsnI GGCC 8 cut(s) 5, 134, 176, 197, 219, 332, 392, 513
Bso31I GGTCTC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 414
Bsp1720I GCTNAGC 1 cut(s) 413
Bsp19I CCATGG 1 cut(s) 129
Bsp68I TCGCGA 1 cut(s) 89
BspACI CCGC 8 cut(s) 20, 62, 165, 291, 312, 342, 450, 516
BspANI GGCC 8 cut(s) 5, 134, 176, 197, 219, 332, 392, 513
BspCNI CTCAG 1 cut(s) 426
BspFNI CGCG 2 cut(s) 22, 89
BspLI GGNNCC 7 cut(s) 157, 163, 268, 274, 430, 499, 514
BspT107I GGYRCC 5 cut(s) 161, 266, 272, 428, 497
BspTNI GGTCTC 1 cut(s) 206
BsrFI RCCGGY 4 cut(s) 197, 269, 494, 500
BssAI RCCGGY 4 cut(s) 197, 269, 494, 500
BssECI CCNNGG 2 cut(s) 129, 374
BssNI GRCGYC 3 cut(s) 267, 273, 429
BssT1I CCWWGG 1 cut(s) 129
Bst6I CTCTTC 2 cut(s) 122, 133
BstACI GRCGYC 3 cut(s) 267, 273, 429
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 4 cut(s) 244, 271, 390, 419
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 57, 381, 465
BstFNI CGCG 2 cut(s) 22, 89
BstH2I RGCGCY 3 cut(s) 270, 276, 432
BstHHI GCGC 3 cut(s) 269, 275, 431
BstMAI GTCTC 2 cut(s) 186, 206
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 5 cut(s) 173, 338, 385, 418, 510
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 1 cut(s) 227
BstUI CGCG 2 cut(s) 22, 89
BstV1I GCAGC 2 cut(s) 201, 408
BstV2I GAAGAC 1 cut(s) 365
BstZI CGGCCG 1 cut(s) 217
BsuRI GGCC 8 cut(s) 5, 134, 176, 197, 219, 332, 392, 513
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 2 cut(s) 70, 424
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 3 cut(s) 57, 381, 465
BtuMI TCGCGA 1 cut(s) 89
Cac8I GCNNGC 4 cut(s) 244, 271, 390, 419
CfoI GCGC 3 cut(s) 269, 275, 431
Cfr10I RCCGGY 4 cut(s) 197, 269, 494, 500
Cfr13I GGNCC 1 cut(s) 512
CseI GACGC 1 cut(s) 11
Csp6I GTAC 1 cut(s) 250
CviAII CATG 2 cut(s) 130, 475
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 413
DinI GGCGCC 3 cut(s) 268, 274, 430
EaeI YGGCCR 5 cut(s) 3, 132, 217, 330, 390
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 122, 133
EarI CTCTTC 2 cut(s) 122, 133
Ecl136II GAGCTC 1 cut(s) 412
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 206
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 412
Eco72I CACGTG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 414
EgeI GGCGCC 3 cut(s) 268, 274, 430
EheI GGCGCC 3 cut(s) 268, 274, 430
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 2 cut(s) 133, 478
FaiI YATR 4 cut(s) 72, 131, 458, 476
FatI CATG 2 cut(s) 129, 474
FauI CCCGC 2 cut(s) 69, 523
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 6 cut(s) 190, 291, 312, 342, 422, 451
FokI GGATG 3 cut(s) 44, 368, 452
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 6 cut(s) 190, 291, 312, 342, 422, 451
FspBI CTAG 1 cut(s) 526
GlaI GCGC 3 cut(s) 268, 274, 430
GluI GCNGC 6 cut(s) 190, 291, 312, 342, 422, 451
HaeII RGCGCY 3 cut(s) 270, 276, 432
HaeIII GGCC 8 cut(s) 5, 134, 176, 197, 219, 332, 392, 513
HapII CCGG 8 cut(s) 135, 159, 198, 229, 270, 333, 495, 501
HgaI GACGC 1 cut(s) 11
HhaI GCGC 3 cut(s) 269, 275, 431
Hin1I GRCGYC 3 cut(s) 267, 273, 429
Hin1II CATG 2 cut(s) 133, 478
Hin6I GCGC 3 cut(s) 267, 273, 429
HinP1I GCGC 3 cut(s) 267, 273, 429
HinfI GANTC 2 cut(s) 102, 125
HpaII CCGG 8 cut(s) 135, 159, 198, 229, 270, 333, 495, 501
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 124, 186
Hpy188III TCNNGA 3 cut(s) 88, 235, 397
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 2 cut(s) 218, 407
HpyAV CCTTC 1 cut(s) 166
HpyCH4IV ACGT 2 cut(s) 179, 346
HpyCH4V TGCA 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 338, 385, 418, 510
HpyF3I CTNAG 1 cut(s) 413
HpySE526I ACGT 2 cut(s) 179, 346
Hsp92I GRCGYC 3 cut(s) 267, 273, 429
Hsp92II CATG 2 cut(s) 133, 478
HspAI GCGC 3 cut(s) 267, 273, 429
KasI GGCGCC 3 cut(s) 266, 272, 428
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
LmnI GCTCC 4 cut(s) 161, 186, 409, 483
Lsp1109I GCAGC 2 cut(s) 201, 408
MaeI CTAG 1 cut(s) 526
MaeII ACGT 2 cut(s) 179, 346
MaeIII GTNAC 3 cut(s) 23, 347, 433
MboII GAAGA 3 cut(s) 109, 150, 370
MhlI GDGCHC 1 cut(s) 414
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 94, 206, 326
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 3 cut(s) 267, 273, 429
MnlI CCTC 9 cut(s) 41, 134, 296, 310, 317, 357, 369, 400, 491
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 269
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 8 cut(s) 135, 159, 198, 229, 270, 333, 495, 501
MspR9I CCNGG 1 cut(s) 229
MvnI CGCG 2 cut(s) 22, 89
MwoI GCNNNNNNNGC 5 cut(s) 173, 338, 385, 418, 510
NaeI GCCGGC 1 cut(s) 271
NarI GGCGCC 3 cut(s) 267, 273, 429
NciI CCSGG 1 cut(s) 229
NcoI CCATGG 1 cut(s) 129
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 133, 478
NlaIV GGNNCC 7 cut(s) 157, 163, 268, 274, 430, 499, 514
NmeAIII GCCGAG 3 cut(s) 280, 399, 405
NmuCI GTSAC 3 cut(s) 23, 347, 433
NruI TCGCGA 1 cut(s) 89
NspI RCATGY 1 cut(s) 478
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 102, 125
PkrI GCNGC 6 cut(s) 191, 292, 313, 343, 423, 452
PluTI GGCGCC 3 cut(s) 270, 276, 432
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 347
Psp124BI GAGCTC 1 cut(s) 414
PspCI CACGTG 1 cut(s) 347
PspN4I GGNNCC 7 cut(s) 157, 163, 268, 274, 430, 499, 514
PspPI GGNCC 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 388
RruI TCGCGA 1 cut(s) 89
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SacI GAGCTC 1 cut(s) 414
SatI GCNGC 6 cut(s) 190, 291, 312, 342, 422, 451
Sau96I GGNCC 1 cut(s) 512
ScrFI CCNGG 1 cut(s) 229
SduI GDGCHC 1 cut(s) 414
SetI ASST 9 cut(s) 182, 244, 288, 309, 321, 349, 390, 414, 423
SfoI GGCGCC 3 cut(s) 268, 274, 430
SgrAI CRCCGGYG 1 cut(s) 269
Sse9I AATT 3 cut(s) 94, 206, 326
SsiI CCGC 8 cut(s) 20, 62, 165, 291, 312, 342, 450, 516
SspDI GGCGCC 3 cut(s) 266, 272, 428
SspMI CTAG 1 cut(s) 526
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 227
StyI CCWWGG 1 cut(s) 129
TaiI ACGT 2 cut(s) 182, 349
TasI AATT 3 cut(s) 94, 206, 326
TauI GCSGC 4 cut(s) 293, 314, 344, 453
TfiI GAWTC 2 cut(s) 102, 125
TseFI GTSAC 3 cut(s) 23, 347, 433
TseI GCWGC 2 cut(s) 189, 421
Tsp45I GTSAC 3 cut(s) 23, 347, 433
TspDTI ATGAA 2 cut(s) 59, 67
XceI RCATGY 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.