Rh4BG384100

histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001504
Physical Location & Seq
Forward (+)
53894408 .. 53896574
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG384100.1

Sequence Viewer

Length: 207 bp
ATGTCCAGGTCAAGCAAAGCTGGGCTTCAATTTTCAGTGGGTAGGATTGCCCGGTTTCTTAAGTCCGGCCAGTACGCCGAGCGTGTCGGCGCCGGAGCTCCGGTGTATCTTTCGGCTATCCTTGAATACCTGGCTGCCGAGGTTTTGGAGCTTGCTGGCAACGCAGCTAGGGACAACAAGAAGAACCAGATGATCTTTGCACAATGA

Protein Analysis

68

Amino Acids

7.33

Weight (kDa)

9.87

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 2 - 62 1.2e-06 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 67
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 1 cut(s) 74
AflII CTTAAG 1 cut(s) 59
AgsI TTSAA 2 cut(s) 29, 125
AjnI CCWGG 2 cut(s) 5, 129
AluBI AGCT 4 cut(s) 20, 98, 151, 167
AluI AGCT 4 cut(s) 20, 98, 151, 167
Alw21I GWGCWC 1 cut(s) 100
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 2 cut(s) 134, 164
AspLEI GCGC 1 cut(s) 92
AsuC2I CCSGG 1 cut(s) 52
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 100
BbvI GCAGC 2 cut(s) 121, 176
BciT130I CCWGG 2 cut(s) 7, 131
BcnI CCSGG 1 cut(s) 52
BfaI CTAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 59
BisI GCNGC 2 cut(s) 135, 165
BlsI GCNGC 2 cut(s) 136, 166
Bme1390I CCNGG 3 cut(s) 7, 52, 131
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 3 cut(s) 7, 52, 131
BpuMI CCSGG 1 cut(s) 52
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bse1I ACTGG 1 cut(s) 70
BseBI CCWGG 2 cut(s) 7, 131
BseDI CCNNGG 1 cut(s) 138
BseNI ACTGG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 121, 176
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 1 cut(s) 69
BshNI GGYRCC 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 100
BsiSI CCGG 4 cut(s) 52, 66, 93, 101
BslFI GGGAC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 1 cut(s) 192
BspANI GGCC 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 59
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 1 cut(s) 192
BssNI GRCGYC 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 7, 131
BstACI GRCGYC 1 cut(s) 90
BstAFI CTTAAG 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 153, 157
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 195
BstMBI GATC 1 cut(s) 192
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 2 cut(s) 7, 131
BstSCI CCNGG 3 cut(s) 5, 50, 129
BstV1I GCAGC 2 cut(s) 121, 176
BsuRI GGCC 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 2 cut(s) 153, 157
CfoI GCGC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 73
CviJI RGCY 8 cut(s) 20, 25, 69, 98, 116, 134, 151, 167
CviKI_1 RGCY 8 cut(s) 20, 25, 69, 98, 116, 134, 151, 167
CviQI GTAC 1 cut(s) 73
DinI GGCGCC 1 cut(s) 91
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 67
Ecl136II GAGCTC 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 98
EcoRII CCWGG 2 cut(s) 5, 129
EcoT38I GRGCYC 1 cut(s) 100
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 185
Fnu4HI GCNGC 2 cut(s) 135, 165
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 2 cut(s) 135, 165
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 91
GluI GCNGC 2 cut(s) 135, 165
GsaI CCCAGC 1 cut(s) 24
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 1 cut(s) 69
HapII CCGG 4 cut(s) 52, 66, 93, 101
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 90
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HpaII CCGG 4 cut(s) 52, 66, 93, 101
HpyCH4V TGCA 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
Hsp92I GRCGYC 1 cut(s) 90
HspAI GCGC 1 cut(s) 90
KasI GGCGCC 1 cut(s) 89
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 3 cut(s) 95, 103, 148
Lsp1109I GCAGC 2 cut(s) 121, 176
MaeI CTAG 1 cut(s) 168
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 1 cut(s) 29
Mly113I GGCGCC 1 cut(s) 90
MnlI CCTC 1 cut(s) 133
MseI TTAA 1 cut(s) 60
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 4 cut(s) 52, 66, 93, 101
MspR9I CCNGG 3 cut(s) 7, 52, 131
MvaI CCWGG 2 cut(s) 7, 131
MwoI GCNNNNNNNGC 1 cut(s) 161
NarI GGCGCC 1 cut(s) 90
NciI CCSGG 1 cut(s) 52
NdeII GATC 1 cut(s) 192
NlaIV GGNNCC 1 cut(s) 91
NmeAIII GCCGAG 2 cut(s) 103, 163
PkrI GCNGC 2 cut(s) 136, 166
PluTI GGCGCC 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 5, 129
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 2 cut(s) 5, 129
PspN4I GGNNCC 1 cut(s) 91
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SacI GAGCTC 1 cut(s) 100
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 2 cut(s) 135, 165
Sau3AI GATC 1 cut(s) 192
ScrFI CCNGG 3 cut(s) 7, 52, 131
SduI GDGCHC 1 cut(s) 100
SetI ASST 7 cut(s) 11, 22, 100, 132, 144, 153, 169
SfoI GGCGCC 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 1 cut(s) 29
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 1 cut(s) 168
SstI GAGCTC 1 cut(s) 100
StyD4I CCNGG 3 cut(s) 5, 50, 129
TasI AATT 1 cut(s) 29
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 1 cut(s) 42
TseI GCWGC 2 cut(s) 134, 164
TspRI CASTG 1 cut(s) 42
Vha464I CTTAAG 1 cut(s) 59
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.