Rorug05G0310600

Histone 2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001504
Physical Location & Seq
Reverse (-)
36480340 .. 36485122
4783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0310600.1

Sequence Viewer

Length: 2241 bp
ATGCATGATTTAAATTCAAATTTCATTTTCCTTTTCATTTTGCAGCACTATATTGTTTACATGGGGCACCACTCGCACCTCAACTCGGAATCTGTCATCAGAGCTAACCATGAGATTCTTGCTTCAGTCACCGGCAGTACCGGTGGACAAGAAGCAGCACTTAACCATTACAGTAAAAGTTTCCAAGGCTTCTCAGCCAAGCTTACACCAGATCAAGCTCAACAACTTGCTGAACATGATTCGGTTATTTCGGTGTTTGAGAGCAAGACAAAAAGACTGAGCACAACACATTCCTGGGATTTTCTAGGCGTAGACGCTACTCCTAATTACAATCAAATGCCAATGGGCTCCAAATCCAATGTCATTGTTGGTGTAATTGACAGTGGAGTTTGGCCAGAGTCAGAGAGCTTCAATGATGAAGGATTAGGACCTGTGCCAGCTAAATTCAGAGGAGAGTGTGTTACTGGTCAGAATTTTACATTGGCCAACTGCAACAGAAAAATCATAGGCTCTCGCTTCTATTATCATGGATTTGAAGTAGAAAATGGGCCACTTGAGTCCTTTGCACCTCTTCCTTTCTTCAGATCAGCTCGAGATGCTGATGGACATGGGAGTCACACTGCCTCCACGATAGCTGGATCTGCAGTAACTAATGTCAGCTTCTTGGGAATGGCCACAGGAACTGCAAGAGGCGGTGCAGAAAGTGCCAGACTTGCTATCTACAAGGCATGTTGGTTCGGCTTGTGTAGTGATGCAGACCTTCTTTCAGCTCTGGATGATGCCATTGGTGATGGTGTCGATATTCTGTCCCTTTCCCTTGGCCCTGATCCTCCACAGCCAACATATTTTGAAGATGCAGTCTCCATTGGAGCTTTCCATGCATTCCAGAAAGGAATACTTGTTTCTGCATCAGCAGGGAACTCTGGTTTCCCTAGTACTGCATGCAATGTAGCTCCCTGGATTCTCACTGTTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACGTTTATCTGGGAAATTCACAAATACTAAAGGGTTCCTCTTTAAACCCACTGAAGATGGATAAGTCTTACAGTTTGATTGCTGCAAGTGTTGCAGCTGCCCCTGGAGTCACAGCAAAGAATGCAAGCTTCTGCAAGGACAATACTCTAAATGCAAGCTTAATTAATGGAAAAATCGTTGTGTGCACCTTTGAGACATTTACCGACAACCGAAGGGAAAAAAGTATAGTTGTAAGACAAGGTGGTGGTGTAGGAATGATTCTTGTGGATCCATTTCTCAAAGATGTTGGCTTTGAGTTTGTCATCCCGGCCACCCTAATTGGTCAAGAAGAAGCAGCACAGCTTCAAGCATACATGACAACAGAGAAGTACCCAGTTGCTAGAATCTCACAAACGACAACAATTCTGAATACTAAACCTGCACCTGAGATGGCAGTGTTCTCCTCCATGGGGCCAAATATCATAACCCCAGACATTATCAAACCAGATATTACAGGACCTGGGGTGAATATCTTAGCAGCATGGTCTCCGGTCTCAACTACACCCACAGCAGAAAGGTCTGTCAATTATAACATCATTTCTGGCACCTCGATGTCTTGTCCACATGTATCTGCAGTTGCAGCAACTTTGAAATCATACCGACCTTCCTGGAGTCCAGCAGCCATAATGTCTGCAATAATGACAACAGCAACAGCTCTAGACAACAACAAACGCCCTATTGGGAGAGATCCAAATGGCACCCCTACCACACCTTTTGACTACGGATCTGGACACATTAACCCAGTTGCAGCAATTGATCCTGGACTTGTATATGATTTTGATTCCCATGACATAATCGACTTTCTCTGCAGCACTGGGGCTAGCACTCTGCAACTGAAAAACCTCACGGGTAATCTAGTTTATTGCCAGAAGCCTTCTATACCTTCCTACAATTTTAACTACCCTTCCATTGGCGTGTCAAAAATGAGTGGAAGGCTATCTGTTCAGCGTACAGTTACTTATTACGGCAAAGGCCCAACGGTATATGCTGCATATGTAGACTACCCAGCAGGTGTAAATGTTACAGTAACACCAGCTAAACTCAAGTTTACAAATACCGGGGAAAAGATGTCTTTCTGTGTTGATTTCACCCCCCTCAAGAATAGTAATGGAAGCTTTGTGTTTGGAGCTCTGACATGGAGTAATGGTATTCAGAATGTTAGGAGTCCTATTGGTCTTAATGTGCTTTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

746

Amino Acids

79.66

Weight (kDa)

6.1

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 17 - 92 3.7e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 119 - 573 3.2e-43 Subtilase family
PA PF02225 374 - 450 5.4e-07 PA domain
fn3_6 PF17766 646 - 739 4.4e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1575
AarI CACCTGC 1 cut(s) 2045
AasI GACNNNNNNGTC 1 cut(s) 612
Acc36I ACCTGC 2 cut(s) 1432, 2045
AccB1I GGYRCC 3 cut(s) 66, 1589, 1742
AccI GTMKAC 2 cut(s) 312, 2043
AciI CCGC 1 cut(s) 693
AclI AACGTT 1 cut(s) 1008
AclWI GGATC 7 cut(s) 646, 821, 1268, 1281, 1727, 1777, 1796
AcoI YGGCCR 4 cut(s) 392, 483, 672, 1314
AcsI RAATTY 6 cut(s) 13, 19, 443, 472, 995, 1021
AcuI CTGAAG 3 cut(s) 108, 565, 1079
AfaI GTAC 4 cut(s) 139, 937, 1376, 1996
AfiI CCNNNNNNNGG 3 cut(s) 85, 1455, 1955
AflIII ACRYGT 1 cut(s) 1609
AgeI ACCGGT 1 cut(s) 140
AgsI TTSAA 8 cut(s) 18, 412, 536, 851, 1005, 1352, 1636, 2235
AjnI CCWGG 6 cut(s) 293, 956, 1108, 1504, 1652, 1804
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
Alw21I GWGCWC 3 cut(s) 284, 1193, 2176
Alw26I GTCTC 4 cut(s) 865, 1193, 1536, 1543
Alw44I GTGCAC 1 cut(s) 1189
AlwI GGATC 7 cut(s) 646, 821, 1268, 1281, 1727, 1777, 1796
AlwNI CAGNNNCTG 2 cut(s) 683, 1505
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 8 cut(s) 392, 483, 548, 672, 820, 1314, 1457, 2017
ApaLI GTGCAC 1 cut(s) 1189
ApoI RAATTY 6 cut(s) 13, 19, 443, 472, 995, 1021
ArsI GACNNNNNNTTYG 2 cut(s) 345, 377
AseI ATTAAT 1 cut(s) 1170
AsiGI ACCGGT 1 cut(s) 140
AspS9I GGNCC 6 cut(s) 428, 548, 821, 1457, 1502, 2018
AsuC2I CCSGG 2 cut(s) 1313, 2104
AsuHPI GGTGA 4 cut(s) 121, 800, 1522, 2125
AsuNHI GCTAGC 1 cut(s) 1865
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 2 cut(s) 428, 1502
BaeGI GKGCMC 2 cut(s) 69, 1193
BalI TGGCCA 3 cut(s) 394, 485, 674
BamHI GGATCC 1 cut(s) 1273
BanI GGYRCC 3 cut(s) 66, 1589, 1742
BanII GRGCYC 2 cut(s) 350, 2176
Bbv12I GWGCWC 3 cut(s) 284, 1193, 2176
BccI CCATC 4 cut(s) 596, 785, 1057, 1429
BceAI ACGGC 1 cut(s) 2026
BciT130I CCWGG 6 cut(s) 295, 958, 1110, 1506, 1654, 1806
BcnI CCSGG 2 cut(s) 1313, 2104
BcoDI GTCTC 4 cut(s) 865, 1193, 1536, 1543
BfaI CTAG 6 cut(s) 305, 933, 1386, 1703, 1866, 1901
BfmI CTRYAG 3 cut(s) 642, 1617, 1852
BfuAI ACCTGC 2 cut(s) 1432, 2045
BmcAI AGTACT 1 cut(s) 937
Bme1390I CCNGG 8 cut(s) 295, 958, 1110, 1313, 1506, 1654, 1806, 2104
Bme18I GGWCC 2 cut(s) 428, 1502
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 6 cut(s) 428, 548, 821, 1457, 1502, 2018
BmiI GGNNCC 7 cut(s) 68, 349, 1042, 1275, 1458, 1591, 1744
BmrFI CCNGG 8 cut(s) 295, 958, 1110, 1313, 1506, 1654, 1806, 2104
BmrI ACTGGG 3 cut(s) 1373, 1781, 1869
BmsI GCATC 5 cut(s) 586, 742, 769, 844, 917
BmtI GCTAGC 1 cut(s) 1869
BmuI ACTGGG 3 cut(s) 1373, 1781, 1869
BpmI CTGGAG 2 cut(s) 1131, 1675
BpuEI CTTGAG 3 cut(s) 575, 2072, 2126
BpuMI CCSGG 2 cut(s) 1313, 2104
BsaI GGTCTC 2 cut(s) 1536, 1543
BsaJI CCNNGG 8 cut(s) 184, 294, 817, 956, 1108, 1452, 1505, 2103
BsaWI WCCGGW 2 cut(s) 140, 1534
BsaXI ACNNNNNCTCC 6 cut(s) 444, 474, 608, 638, 2176, 2206
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 1455, 1955
Bse118I RCCGGY 2 cut(s) 131, 140
Bse1I ACTGG 4 cut(s) 469, 1379, 1787, 1864
Bse3DI GCAATG 1 cut(s) 952
BseBI CCWGG 6 cut(s) 295, 958, 1110, 1506, 1654, 1806
BseDI CCNNGG 8 cut(s) 184, 294, 817, 956, 1108, 1452, 1505, 2103
BseGI GGATG 2 cut(s) 781, 1308
BseLI CCNNNNNNNGG 3 cut(s) 85, 1455, 1955
BseMI GCAATG 1 cut(s) 952
BseMII CTCAG 3 cut(s) 207, 269, 1422
BseNI ACTGG 4 cut(s) 469, 1379, 1787, 1864
BseRI GAGGAG 2 cut(s) 465, 1438
BseSI GKGCMC 2 cut(s) 69, 1193
BseYI CCCAGC 1 cut(s) 2050
BsgI GTGCAG 2 cut(s) 717, 1410
BshFI GGCC 8 cut(s) 394, 485, 550, 674, 822, 1316, 1459, 2019
BshNI GGYRCC 3 cut(s) 66, 1589, 1742
BshTI ACCGGT 1 cut(s) 140
BsiHKAI GWGCWC 3 cut(s) 284, 1193, 2176
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 5 cut(s) 132, 141, 1313, 1535, 2103
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 3 cut(s) 85, 1455, 1955
BsmAI GTCTC 4 cut(s) 865, 1193, 1536, 1543
BsmFI GGGAC 1 cut(s) 793
BsmI GAATGC 2 cut(s) 881, 1132
BsnI GGCC 8 cut(s) 394, 485, 550, 674, 822, 1316, 1459, 2019
Bso31I GGTCTC 2 cut(s) 1536, 1543
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 5 cut(s) 69, 284, 350, 1193, 2176
Bsp143I GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
Bsp19I CCATGG 1 cut(s) 1452
BspACI CCGC 1 cut(s) 693
BspANI GGCC 8 cut(s) 394, 485, 550, 674, 822, 1316, 1459, 2019
BspCNI CTCAG 3 cut(s) 206, 270, 1423
BspLI GGNNCC 7 cut(s) 68, 349, 1042, 1275, 1458, 1591, 1744
BspMAI CTGCAG 3 cut(s) 646, 1621, 1856
BspMI ACCTGC 2 cut(s) 1432, 2045
BspOI GCTAGC 1 cut(s) 1869
BspPI GGATC 7 cut(s) 646, 821, 1268, 1281, 1727, 1777, 1796
BspT107I GGYRCC 3 cut(s) 66, 1589, 1742
BspTNI GGTCTC 2 cut(s) 1536, 1543
BsrDI GCAATG 1 cut(s) 952
BsrFI RCCGGY 2 cut(s) 131, 140
BsrI ACTGG 4 cut(s) 469, 1379, 1787, 1864
BssAI RCCGGY 2 cut(s) 131, 140
BssECI CCNNGG 8 cut(s) 184, 294, 817, 956, 1108, 1452, 1505, 2103
BssMI GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
BssT1I CCWWGG 3 cut(s) 184, 817, 1452
Bst2UI CCWGG 6 cut(s) 295, 958, 1110, 1506, 1654, 1806
Bst4CI ACNGT 7 cut(s) 173, 383, 970, 1079, 1999, 2026, 2071
Bst6I CTCTTC 1 cut(s) 576
BstAPI GCANNNNNTGC 3 cut(s) 704, 1097, 1127
BstC8I GCNNGC 6 cut(s) 438, 943, 979, 1132, 1162, 1867
BstDEI CTNAG 4 cut(s) 193, 278, 1431, 1519
BstDSI CCRYGG 1 cut(s) 1452
BstF5I GGATG 2 cut(s) 781, 1308
BstKTI GATC 8 cut(s) 214, 587, 641, 829, 1276, 1735, 1772, 1804
BstMAI GTCTC 4 cut(s) 865, 1193, 1536, 1543
BstMBI GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
BstMWI GCNNNNNNNGC 9 cut(s) 73, 596, 641, 704, 713, 878, 1097, 1127, 1625
BstNI CCWGG 6 cut(s) 295, 958, 1110, 1506, 1654, 1806
BstNSI RCATGY 3 cut(s) 732, 945, 1613
BstSCI CCNGG 8 cut(s) 293, 956, 1108, 1311, 1504, 1652, 1804, 2102
BstSFI CTRYAG 3 cut(s) 642, 1617, 1852
BstSLI GKGCMC 2 cut(s) 69, 1193
BstX2I RGATCY 4 cut(s) 638, 1273, 1732, 1769
BstYI RGATCY 4 cut(s) 638, 1273, 1732, 1769
BsuRI GGCC 8 cut(s) 394, 485, 550, 674, 822, 1316, 1459, 2019
BtgI CCRYGG 1 cut(s) 1452
BtsCI GGATG 2 cut(s) 781, 1308
BtsI GCAGTG 2 cut(s) 618, 1446
BtsIMutI CAGTG 6 cut(s) 388, 618, 966, 1055, 1446, 1857
BveI ACCTGC 2 cut(s) 1432, 2045
Cac8I GCNNGC 6 cut(s) 438, 943, 979, 1132, 1162, 1867
CaiI CAGNNNCTG 2 cut(s) 683, 1505
Cfr10I RCCGGY 2 cut(s) 131, 140
Cfr13I GGNCC 6 cut(s) 428, 548, 821, 1457, 1502, 2018
CseI GACGC 1 cut(s) 323
Csp6I GTAC 4 cut(s) 138, 936, 1375, 1995
CspAI ACCGGT 1 cut(s) 140
CspCI CAANNNNNGTGG 4 cut(s) 1306, 1341, 1741, 1776
CviQI GTAC 4 cut(s) 138, 936, 1375, 1995
DdeI CTNAG 4 cut(s) 193, 278, 1431, 1519
DpnI GATC 8 cut(s) 213, 586, 640, 828, 1275, 1734, 1771, 1803
DpnII GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
DraI TTTAAA 2 cut(s) 12, 1050
DrdI GACNNNNNNGTC 1 cut(s) 612
DseDI GACNNNNNNGTC 1 cut(s) 612
EaeI YGGCCR 4 cut(s) 392, 483, 672, 1314
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Ecl136II GAGCTC 1 cut(s) 2174
Eco130I CCWWGG 3 cut(s) 184, 817, 1452
Eco24I GRGCYC 2 cut(s) 350, 2176
Eco31I GGTCTC 2 cut(s) 1536, 1543
Eco47I GGWCC 2 cut(s) 428, 1502
Eco53kI GAGCTC 1 cut(s) 2174
Eco57I CTGAAG 3 cut(s) 108, 565, 1079
Eco88I CYCGRG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 2174
EcoO109I RGGNCCY 2 cut(s) 428, 1502
EcoRI GAATTC 1 cut(s) 995
EcoRII CCWGG 6 cut(s) 293, 956, 1108, 1504, 1652, 1804
EcoT14I CCWWGG 3 cut(s) 184, 817, 1452
EcoT22I ATGCAT 2 cut(s) 6, 883
EcoT38I GRGCYC 2 cut(s) 350, 2176
ErhI CCWWGG 3 cut(s) 184, 817, 1452
FalI AAGNNNNNCTT 4 cut(s) 144, 176, 882, 914
FaqI GGGAC 1 cut(s) 793
FauNDI CATATG 1 cut(s) 2038
FblI GTMKAC 2 cut(s) 312, 2043
FokI GGATG 2 cut(s) 788, 1295
FriOI GRGCYC 2 cut(s) 350, 2176
FspBI CTAG 6 cut(s) 305, 933, 1386, 1703, 1866, 1901
GsaI CCCAGC 1 cut(s) 2054
GsuI CTGGAG 2 cut(s) 1131, 1675
HaeIII GGCC 8 cut(s) 394, 485, 550, 674, 822, 1316, 1459, 2019
HapII CCGG 5 cut(s) 132, 141, 1313, 1535, 2103
HgaI GACGC 1 cut(s) 323
HindIII AAGCTT 4 cut(s) 200, 1132, 1162, 2158
HpaII CCGG 5 cut(s) 132, 141, 1313, 1535, 2103
HphI GGTGA 4 cut(s) 121, 800, 1522, 2125
Hpy166II GTNNAC 7 cut(s) 58, 146, 313, 1191, 1607, 2044, 2094
Hpy188I TCNGA 9 cut(s) 88, 101, 403, 449, 471, 584, 1413, 2178, 2199
Hpy188III TCNNGA 7 cut(s) 593, 773, 886, 1331, 1703, 1773, 2143
Hpy8I GTNNAC 7 cut(s) 58, 146, 313, 1191, 1607, 2044, 2094
HpyAV CCTTC 8 cut(s) 413, 770, 1212, 1659, 1929, 1938, 1959, 1971
HpyCH4III ACNGT 7 cut(s) 173, 383, 970, 1079, 1999, 2026, 2071
HpyCH4IV ACGT 1 cut(s) 1008
HpyF10VI GCNNNNNNNGC 9 cut(s) 73, 596, 641, 704, 713, 878, 1097, 1127, 1625
HpyF3I CTNAG 4 cut(s) 193, 278, 1431, 1519
HpySE526I ACGT 1 cut(s) 1008
Kzo9I GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
LmnI GCTCC 4 cut(s) 353, 869, 958, 2171
LweI GCATC 5 cut(s) 586, 742, 769, 844, 917
MaeI CTAG 6 cut(s) 305, 933, 1386, 1703, 1866, 1901
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 8 cut(s) 127, 460, 614, 646, 1114, 1999, 2065, 2071
MalI GATC 8 cut(s) 213, 586, 640, 828, 1275, 1734, 1771, 1803
MboI GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
MboII GAAGA 5 cut(s) 563, 571, 863, 1072, 1346
MfeI CAATTG 1 cut(s) 1797
MflI RGATCY 4 cut(s) 638, 1273, 1732, 1769
MhlI GDGCHC 5 cut(s) 69, 284, 350, 1193, 2176
MlsI TGGCCA 3 cut(s) 394, 485, 674
MluNI TGGCCA 3 cut(s) 394, 485, 674
MlyI GAGTC 6 cut(s) 407, 566, 622, 1122, 1666, 2218
Mox20I TGGCCA 3 cut(s) 394, 485, 674
Mph1103I ATGCAT 2 cut(s) 6, 883
MscI TGGCCA 3 cut(s) 394, 485, 674
MseI TTAA 8 cut(s) 11, 162, 1049, 1166, 1170, 1782, 1941, 2223
MslI CAYNNNNRTG 2 cut(s) 1595, 1958
Msp20I TGGCCA 3 cut(s) 394, 485, 674
MspA1I CMGCKG 1 cut(s) 1103
MspI CCGG 5 cut(s) 132, 141, 1313, 1535, 2103
MspR9I CCNGG 8 cut(s) 295, 958, 1110, 1313, 1506, 1654, 1806, 2104
MunI CAATTG 1 cut(s) 1797
Mva1269I GAATGC 2 cut(s) 881, 1132
MvaI CCWGG 6 cut(s) 295, 958, 1110, 1506, 1654, 1806
MwoI GCNNNNNNNGC 9 cut(s) 73, 596, 641, 704, 713, 878, 1097, 1127, 1625
NciI CCSGG 2 cut(s) 1313, 2104
NcoI CCATGG 1 cut(s) 1452
NdeI CATATG 1 cut(s) 2038
NdeII GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
NheI GCTAGC 1 cut(s) 1865
NlaIV GGNNCC 7 cut(s) 68, 349, 1042, 1275, 1458, 1591, 1744
NmuCI GTSAC 3 cut(s) 127, 614, 1114
NsiI ATGCAT 2 cut(s) 6, 883
NspI RCATGY 3 cut(s) 732, 945, 1613
PacI TTAATTAA 1 cut(s) 1170
PaeI GCATGC 1 cut(s) 945
PaeR7I CTCGAG 1 cut(s) 591
PaqCI CACCTGC 1 cut(s) 2045
PciI ACATGT 1 cut(s) 1609
PctI GAATGC 2 cut(s) 881, 1132
PfeI GAWTC 7 cut(s) 89, 115, 239, 961, 1264, 1389, 1826
PfoI TCCNGGA 2 cut(s) 1652, 1804
PinAI ACCGGT 1 cut(s) 140
PleI GAGTC 6 cut(s) 406, 565, 621, 1121, 1665, 2217
PpsI GAGTC 6 cut(s) 406, 565, 621, 1121, 1665, 2217
PpuMI RGGWCCY 2 cut(s) 428, 1502
PscI ACATGT 1 cut(s) 1609
PshBI ATTAAT 1 cut(s) 1170
PsiI TTATAA 1 cut(s) 1575
Psp124BI GAGCTC 1 cut(s) 2176
Psp1406I AACGTT 1 cut(s) 1008
Psp5II RGGWCCY 2 cut(s) 428, 1502
Psp6I CCWGG 6 cut(s) 293, 956, 1108, 1504, 1652, 1804
PspFI CCCAGC 1 cut(s) 2050
PspGI CCWGG 6 cut(s) 293, 956, 1108, 1504, 1652, 1804
PspN4I GGNNCC 7 cut(s) 68, 349, 1042, 1275, 1458, 1591, 1744
PspPI GGNCC 6 cut(s) 428, 548, 821, 1457, 1502, 2018
PspPPI RGGWCCY 2 cut(s) 428, 1502
PstI CTGCAG 3 cut(s) 646, 1621, 1856
PstNI CAGNNNCTG 2 cut(s) 683, 1505
PsuI RGATCY 4 cut(s) 638, 1273, 1732, 1769
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 4 cut(s) 139, 937, 1376, 1996
RsaNI GTAC 4 cut(s) 138, 936, 1375, 1995
RseI CAYNNNNRTG 2 cut(s) 1595, 1958
SacI GAGCTC 1 cut(s) 2176
SaqAI TTAA 8 cut(s) 11, 162, 1049, 1166, 1170, 1782, 1941, 2223
Sau3AI GATC 8 cut(s) 211, 584, 638, 826, 1273, 1732, 1769, 1801
Sau96I GGNCC 6 cut(s) 428, 548, 821, 1457, 1502, 2018
ScaI AGTACT 1 cut(s) 937
SchI GAGTC 6 cut(s) 407, 566, 622, 1122, 1666, 2218
ScrFI CCNGG 8 cut(s) 295, 958, 1110, 1313, 1506, 1654, 1806, 2104
SduI GDGCHC 5 cut(s) 69, 284, 350, 1193, 2176
SfaNI GCATC 5 cut(s) 586, 742, 769, 844, 917
SfcI CTRYAG 3 cut(s) 642, 1617, 1852
Sfr274I CTCGAG 1 cut(s) 591
SinI GGWCC 2 cut(s) 428, 1502
SlaI CTCGAG 1 cut(s) 591
SmiI ATTTAAAT 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 1595, 1958
SmlI CTYRAG 4 cut(s) 554, 591, 2087, 2141
SmoI CTYRAG 4 cut(s) 554, 591, 2087, 2141
SphI GCATGC 1 cut(s) 945
SsiI CCGC 1 cut(s) 693
SspMI CTAG 6 cut(s) 305, 933, 1386, 1703, 1866, 1901
SstI GAGCTC 1 cut(s) 2176
StyD4I CCNGG 8 cut(s) 293, 956, 1108, 1311, 1504, 1652, 1804, 2102
StyI CCWWGG 3 cut(s) 184, 817, 1452
SwaI ATTTAAAT 1 cut(s) 12
TaaI ACNGT 7 cut(s) 173, 383, 970, 1079, 1999, 2026, 2071
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 4 cut(s) 592, 798, 1595, 1842
TatI WGTACW 1 cut(s) 935
TfiI GAWTC 7 cut(s) 89, 115, 239, 961, 1264, 1389, 1826
Tru1I TTAA 8 cut(s) 11, 162, 1049, 1166, 1170, 1782, 1941, 2223
Tru9I TTAA 8 cut(s) 11, 162, 1049, 1166, 1170, 1782, 1941, 2223
TscAI CASTG 6 cut(s) 388, 625, 973, 1062, 1446, 1864
TseFI GTSAC 3 cut(s) 127, 614, 1114
Tsp45I GTSAC 3 cut(s) 127, 614, 1114
TspDTI ATGAA 3 cut(s) 13, 25, 432
TspGWI ACGGA 1 cut(s) 1782
TspRI CASTG 6 cut(s) 388, 625, 973, 1062, 1446, 1864
VneI GTGCAC 1 cut(s) 1189
VpaK11BI GGWCC 2 cut(s) 428, 1502
VspI ATTAAT 1 cut(s) 1170
XapI RAATTY 6 cut(s) 13, 19, 443, 472, 995, 1021
XbaI TCTAGA 1 cut(s) 1702
XceI RCATGY 3 cut(s) 732, 945, 1613
XhoI CTCGAG 1 cut(s) 591
XmiI GTMKAC 2 cut(s) 312, 2043
XspI CTAG 6 cut(s) 305, 933, 1386, 1703, 1866, 1901
ZrmI AGTACT 1 cut(s) 937
Zsp2I ATGCAT 2 cut(s) 6, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.