Rh5BG039700

Histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001504
Physical Location & Seq
Forward (+)
2879234 .. 2880808
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG039700.1

Sequence Viewer

Length: 396 bp
ATGGCCGGAAGAGGCAAATCGCTCGGCTCCGGCGCCGCCAAGAAGGCCACCTCCCGCAGCAGCAAAGCCGGCCTCCAATTCCCCGTCGGCCGTATCGCTCGCTTCCTCAAATCCGGCAAGTACGCCGAGCGTGTCGGTGCCGGCGCCCCCGTCTACCTCGCCGCCGTCCTCGAATACCTCGCTGCCGAGGTTTTGGAACTGGCCGGAAATGCAGCCAGGGACAACAAGAAGACTCGGATTGTGCCACGTCACATCCAATTGGCCGTCCGGAACGACGAGGAGCTCGGCAGGCTTCTCGGATCAGTCACCATCGCAAACGGCGGCGTCTTGCCCAACATCCACAACATGCTTCTTCCCAAGAAGACCTCCAAGAGCGCTCCGGCCGACGAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

131

Amino Acids

13.76

Weight (kDa)

10.23

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 13 - 90 1.6e-14 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 93 - 126 9.4e-19 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 32, 137, 143
AccI GTMKAC 1 cut(s) 153
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 4 cut(s) 36, 55, 162, 321
AclWI GGATC 1 cut(s) 307
AcoI YGGCCR 5 cut(s) 3, 88, 201, 261, 381
AcyI GRCGYC 3 cut(s) 33, 144, 324
AfaI GTAC 1 cut(s) 122
AfeI AGCGCT 1 cut(s) 376
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 1 cut(s) 215
AluBI AGCT 1 cut(s) 283
AluI AGCT 1 cut(s) 283
Alw21I GWGCWC 1 cut(s) 285
AlwI GGATC 1 cut(s) 307
Aor13HI TCCGGA 1 cut(s) 267
Aor51HI AGCGCT 1 cut(s) 376
AoxI GGCC 7 cut(s) 3, 45, 70, 88, 201, 261, 381
ApeKI GCWGC 4 cut(s) 57, 60, 182, 212
AspLEI GCGC 3 cut(s) 35, 146, 377
AsuHPI GGTGA 1 cut(s) 298
BanI GGYRCC 3 cut(s) 32, 137, 143
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 2 cut(s) 236, 368
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 4 cut(s) 69, 72, 169, 224
BccI CCATC 1 cut(s) 317
BceAI ACGGC 4 cut(s) 75, 149, 248, 334
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 277, 311
BciT130I CCWGG 1 cut(s) 217
BfoI RGCGCY 3 cut(s) 36, 147, 378
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 213, 322
BlsI GCNGC 7 cut(s) 37, 59, 62, 163, 184, 214, 323
Bme1390I CCNGG 1 cut(s) 217
BmgBI CACGTC 1 cut(s) 248
BmiI GGNNCC 4 cut(s) 28, 34, 139, 145
BmrFI CCNGG 1 cut(s) 217
BpiI GAAGAC 2 cut(s) 236, 368
BsaHI GRCGYC 3 cut(s) 33, 144, 324
BsaJI CCNNGG 2 cut(s) 186, 216
BsaWI WCCGGW 1 cut(s) 267
Bse118I RCCGGY 2 cut(s) 68, 140
Bse1I ACTGG 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 1 cut(s) 217
BseDI CCNNGG 2 cut(s) 186, 216
BseGI GGATG 2 cut(s) 252, 336
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 293
BseX3I CGGCCG 2 cut(s) 88, 381
BseXI GCAGC 4 cut(s) 69, 72, 169, 224
Bsh1285I CGRYCG 2 cut(s) 91, 384
BshFI GGCC 7 cut(s) 5, 47, 72, 90, 203, 263, 383
BshNI GGYRCC 3 cut(s) 32, 137, 143
BsiEI CGRYCG 2 cut(s) 91, 384
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 8 cut(s) 6, 30, 69, 114, 141, 204, 268, 380
BslFI GGGAC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 7 cut(s) 5, 47, 72, 90, 203, 263, 383
Bsp1286I GDGCHC 1 cut(s) 285
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 299
BspACI CCGC 4 cut(s) 36, 55, 162, 321
BspANI GGCC 7 cut(s) 5, 47, 72, 90, 203, 263, 383
BspEI TCCGGA 1 cut(s) 267
BspLI GGNNCC 4 cut(s) 28, 34, 139, 145
BspPI GGATC 1 cut(s) 307
BspT107I GGYRCC 3 cut(s) 32, 137, 143
BsrFI RCCGGY 2 cut(s) 68, 140
BsrI ACTGG 1 cut(s) 204
BssAI RCCGGY 2 cut(s) 68, 140
BssECI CCNNGG 2 cut(s) 186, 216
BssMI GATC 1 cut(s) 299
BssNI GRCGYC 3 cut(s) 33, 144, 324
Bst2UI CCWGG 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 4
BstACI GRCGYC 3 cut(s) 33, 144, 324
BstC8I GCNNGC 4 cut(s) 70, 100, 142, 290
BstF5I GGATG 2 cut(s) 252, 336
BstH2I RGCGCY 3 cut(s) 36, 147, 378
BstHHI GCGC 3 cut(s) 35, 146, 377
BstKTI GATC 1 cut(s) 302
BstMBI GATC 1 cut(s) 299
BstMCI CGRYCG 2 cut(s) 91, 384
BstMWI GCNNNNNNNGC 4 cut(s) 44, 69, 209, 289
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 69, 72, 169, 224
BstV2I GAAGAC 2 cut(s) 236, 368
BstZI CGGCCG 2 cut(s) 88, 381
BsuRI GGCC 7 cut(s) 5, 47, 72, 90, 203, 263, 383
BtgZI GCGATG 1 cut(s) 295
BtrI CACGTC 1 cut(s) 248
BtsCI GGATG 2 cut(s) 252, 336
Cac8I GCNNGC 4 cut(s) 70, 100, 142, 290
CfoI GCGC 3 cut(s) 35, 146, 377
Cfr10I RCCGGY 2 cut(s) 68, 140
CseI GACGC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 121
CviAII CATG 1 cut(s) 346
CviQI GTAC 1 cut(s) 121
DinI GGCGCC 2 cut(s) 34, 145
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
EaeI YGGCCR 5 cut(s) 3, 88, 201, 261, 381
EagI CGGCCG 2 cut(s) 88, 381
Eam1104I CTCTTC 1 cut(s) 4
EarI CTCTTC 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 2 cut(s) 88, 381
Eco24I GRGCYC 1 cut(s) 285
Eco47III AGCGCT 1 cut(s) 376
Eco52I CGGCCG 2 cut(s) 88, 381
Eco53kI GAGCTC 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 215
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 2 cut(s) 34, 145
EheI GGCGCC 2 cut(s) 34, 145
FaeI CATG 1 cut(s) 349
FaiI YATR 1 cut(s) 347
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 345
FauI CCCGC 1 cut(s) 62
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 213, 322
FokI GGATG 2 cut(s) 239, 323
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 213, 322
GlaI GCGC 3 cut(s) 34, 145, 376
GluI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 213, 322
HaeII RGCGCY 3 cut(s) 36, 147, 378
HaeIII GGCC 7 cut(s) 5, 47, 72, 90, 203, 263, 383
HapII CCGG 8 cut(s) 6, 30, 69, 114, 141, 204, 268, 380
HgaI GACGC 1 cut(s) 313
HhaI GCGC 3 cut(s) 35, 146, 377
Hin1I GRCGYC 3 cut(s) 33, 144, 324
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 3 cut(s) 33, 144, 375
HinP1I GCGC 3 cut(s) 33, 144, 375
HinfI GANTC 1 cut(s) 232
HpaII CCGG 8 cut(s) 6, 30, 69, 114, 141, 204, 268, 380
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 237, 299
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 3 cut(s) 89, 278, 389
HpyAV CCTTC 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 69, 209, 289
HpySE526I ACGT 1 cut(s) 247
Hsp92I GRCGYC 3 cut(s) 33, 144, 324
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 3 cut(s) 33, 144, 375
KasI GGCGCC 2 cut(s) 32, 143
Kpn2I TCCGGA 1 cut(s) 267
KroI GCCGGC 2 cut(s) 68, 140
KroNI GCCGGC 2 cut(s) 70, 142
Kzo9I GATC 1 cut(s) 299
LmnI GCTCC 3 cut(s) 32, 280, 382
Lsp1109I GCAGC 4 cut(s) 69, 72, 169, 224
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 2 cut(s) 248, 304
MalI GATC 1 cut(s) 301
MboI GATC 1 cut(s) 299
MboII GAAGA 4 cut(s) 21, 241, 344, 373
MfeI CAATTG 1 cut(s) 257
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 2 cut(s) 77, 257
Mly113I GGCGCC 2 cut(s) 33, 144
MlyI GAGTC 1 cut(s) 226
MroI TCCGGA 1 cut(s) 267
MroNI GCCGGC 2 cut(s) 68, 140
MspI CCGG 8 cut(s) 6, 30, 69, 114, 141, 204, 268, 380
MspR9I CCNGG 1 cut(s) 217
MunI CAATTG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 44, 69, 209, 289
NaeI GCCGGC 2 cut(s) 70, 142
NarI GGCGCC 2 cut(s) 33, 144
NdeII GATC 1 cut(s) 299
NgoMIV GCCGGC 2 cut(s) 68, 140
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 4 cut(s) 28, 34, 139, 145
NmeAIII GCCGAG 3 cut(s) 151, 211, 264
NmuCI GTSAC 2 cut(s) 248, 304
NspI RCATGY 1 cut(s) 349
PdiI GCCGGC 2 cut(s) 70, 142
PkrI GCNGC 7 cut(s) 37, 59, 62, 163, 184, 214, 323
PleI GAGTC 1 cut(s) 226
PluTI GGCGCC 2 cut(s) 36, 147
PpsI GAGTC 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 215
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 4 cut(s) 28, 34, 139, 145
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SacI GAGCTC 1 cut(s) 285
SatI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 213, 322
Sau3AI GATC 1 cut(s) 299
SchI GAGTC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 217
SduI GDGCHC 1 cut(s) 285
SetI ASST 7 cut(s) 53, 159, 180, 192, 250, 285, 368
SfoI GGCGCC 2 cut(s) 34, 145
Sse9I AATT 2 cut(s) 77, 257
SsiI CCGC 4 cut(s) 36, 55, 162, 321
SspDI GGCGCC 2 cut(s) 32, 143
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 215
TaiI ACGT 1 cut(s) 250
TaqI TCGA 1 cut(s) 171
TasI AATT 2 cut(s) 77, 257
TauI GCSGC 3 cut(s) 38, 164, 324
TseFI GTSAC 2 cut(s) 248, 304
TseI GCWGC 4 cut(s) 57, 60, 182, 212
Tsp45I GTSAC 2 cut(s) 248, 304
XceI RCATGY 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.