RchiOBHm_Chr5g0004551

Histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001504
Physical Location & Seq
Forward (+)
2969105 .. 2969780
676 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28578

Sequence Viewer

Length: 441 bp
ATGGCCGGAAGAGGCAAATCGCTCGGCTCCGGCGCCGCCAAGAAGGCCACCTCCCGCAGCAGCAAAGCCGGCCTCCAATTCCCCGTCGGCCGTATCGCTCGCTTCCTCAAATCCGGCAAGTACGCCGAGCGCGTCGGTGCCGGCGCTCCCGTCTACCTGGCCGCCGTCCTCGAATACCTCGCCGCCGAGATAACCGATTCAATCCTTCTCATCTCACTCCGACCGTCAAATTTAGTTTTGGAACTGGCCGGAAATGCAGCCAGGGACAACAAGAAGACTCGGATTGTGCCACGTCACATCCAATTGGCCGTCCGGAACGACGAGGAGCTCGGCAGGCTTCTCGGATCCGTCACCATCGCAAACGGCGGCGTTTTGCCCAACATCCACAACATGCTTCTTCCCAAGAAGACCTCTAAGAGCGCTCCGGCCGACGAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

146

Amino Acids

15.4

Weight (kDa)

10.23

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone_H2A_C PF16211 108 - 141 1.1e-18 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 32, 137
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 312
AciI CCGC 5 cut(s) 36, 55, 162, 183, 366
AclWI GGATC 2 cut(s) 339, 352
AcoI YGGCCR 6 cut(s) 3, 88, 159, 246, 306, 426
AcsI RAATTY 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 122
AfeI AGCGCT 1 cut(s) 421
AgsI TTSAA 1 cut(s) 201
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 2 cut(s) 156, 260
AluBI AGCT 1 cut(s) 328
AluI AGCT 1 cut(s) 328
Alw21I GWGCWC 1 cut(s) 330
AlwI GGATC 2 cut(s) 339, 352
Aor13HI TCCGGA 1 cut(s) 312
Aor51HI AGCGCT 1 cut(s) 421
AoxI GGCC 8 cut(s) 3, 45, 70, 88, 159, 246, 306, 426
ApeKI GCWGC 3 cut(s) 57, 60, 257
ApoI RAATTY 1 cut(s) 229
AspLEI GCGC 4 cut(s) 35, 132, 146, 422
AsuHPI GGTGA 1 cut(s) 343
BamHI GGATCC 1 cut(s) 344
BanI GGYRCC 2 cut(s) 32, 137
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 2 cut(s) 281, 413
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 3 cut(s) 69, 72, 269
BccI CCATC 1 cut(s) 362
BceAI ACGGC 4 cut(s) 75, 149, 293, 379
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 322, 356
BciT130I CCWGG 2 cut(s) 158, 262
BfoI RGCGCY 3 cut(s) 36, 147, 423
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 258, 367
BlsI GCNGC 7 cut(s) 37, 59, 62, 163, 184, 259, 368
Bme1390I CCNGG 2 cut(s) 158, 262
BmgBI CACGTC 1 cut(s) 293
BmiI GGNNCC 4 cut(s) 28, 34, 139, 346
BmrFI CCNGG 2 cut(s) 158, 262
BpiI GAAGAC 2 cut(s) 281, 413
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 261
BsaWI WCCGGW 1 cut(s) 312
Bse118I RCCGGY 2 cut(s) 68, 140
Bse1I ACTGG 1 cut(s) 249
BseAI TCCGGA 1 cut(s) 312
BseBI CCWGG 2 cut(s) 158, 262
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 297, 381
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 338
BseX3I CGGCCG 2 cut(s) 88, 426
BseXI GCAGC 3 cut(s) 69, 72, 269
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 3 cut(s) 91, 224, 429
BshFI GGCC 8 cut(s) 5, 47, 72, 90, 161, 248, 308, 428
BshNI GGYRCC 2 cut(s) 32, 137
BsiEI CGRYCG 3 cut(s) 91, 224, 429
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 8 cut(s) 6, 30, 69, 114, 141, 249, 313, 425
BslFI GGGAC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 8 cut(s) 5, 47, 72, 90, 161, 248, 308, 428
Bsp1286I GDGCHC 1 cut(s) 330
Bsp13I TCCGGA 1 cut(s) 312
Bsp143I GATC 1 cut(s) 344
BspACI CCGC 5 cut(s) 36, 55, 162, 183, 366
BspANI GGCC 8 cut(s) 5, 47, 72, 90, 161, 248, 308, 428
BspEI TCCGGA 1 cut(s) 312
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 4 cut(s) 28, 34, 139, 346
BspPI GGATC 2 cut(s) 339, 352
BspT107I GGYRCC 2 cut(s) 32, 137
BsrFI RCCGGY 2 cut(s) 68, 140
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 2 cut(s) 68, 140
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 344
BssNI GRCGYC 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 158, 262
Bst4CI ACNGT 1 cut(s) 225
Bst6I CTCTTC 1 cut(s) 4
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 4 cut(s) 70, 100, 142, 335
BstDEI CTNAG 1 cut(s) 414
BstF5I GGATG 2 cut(s) 297, 381
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 3 cut(s) 36, 147, 423
BstHHI GCGC 4 cut(s) 35, 132, 146, 422
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstMCI CGRYCG 3 cut(s) 91, 224, 429
BstMWI GCNNNNNNNGC 4 cut(s) 44, 69, 254, 334
BstNI CCWGG 2 cut(s) 158, 262
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 2 cut(s) 156, 260
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 69, 72, 269
BstV2I GAAGAC 2 cut(s) 281, 413
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
BstZI CGGCCG 2 cut(s) 88, 426
BsuRI GGCC 8 cut(s) 5, 47, 72, 90, 161, 248, 308, 428
BtgZI GCGATG 1 cut(s) 340
BtrI CACGTC 1 cut(s) 293
BtsCI GGATG 2 cut(s) 297, 381
Cac8I GCNNGC 4 cut(s) 70, 100, 142, 335
CfoI GCGC 4 cut(s) 35, 132, 146, 422
Cfr10I RCCGGY 2 cut(s) 68, 140
CseI GACGC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 121
CviAII CATG 1 cut(s) 391
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 1 cut(s) 414
DinI GGCGCC 1 cut(s) 34
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
EaeI YGGCCR 6 cut(s) 3, 88, 159, 246, 306, 426
EagI CGGCCG 2 cut(s) 88, 426
Eam1104I CTCTTC 1 cut(s) 4
EarI CTCTTC 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 328
EclXI CGGCCG 2 cut(s) 88, 426
Eco24I GRGCYC 1 cut(s) 330
Eco47III AGCGCT 1 cut(s) 421
Eco52I CGGCCG 2 cut(s) 88, 426
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 156, 260
EcoT38I GRGCYC 1 cut(s) 330
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
FaeI CATG 1 cut(s) 394
FaiI YATR 1 cut(s) 392
FaqI GGGAC 1 cut(s) 278
FatI CATG 1 cut(s) 390
FauI CCCGC 1 cut(s) 62
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 258, 367
FokI GGATG 2 cut(s) 284, 368
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 258, 367
GlaI GCGC 4 cut(s) 34, 131, 145, 421
GluI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 258, 367
HaeII RGCGCY 3 cut(s) 36, 147, 423
HaeIII GGCC 8 cut(s) 5, 47, 72, 90, 161, 248, 308, 428
HapII CCGG 8 cut(s) 6, 30, 69, 114, 141, 249, 313, 425
HgaI GACGC 1 cut(s) 121
HhaI GCGC 4 cut(s) 35, 132, 146, 422
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 4 cut(s) 33, 130, 144, 420
HinP1I GCGC 4 cut(s) 33, 130, 144, 420
HinfI GANTC 2 cut(s) 197, 277
HpaII CCGG 8 cut(s) 6, 30, 69, 114, 141, 249, 313, 425
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 221, 282, 344
Hpy188III TCNNGA 1 cut(s) 313
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 4 cut(s) 89, 137, 323, 434
HpyAV CCTTC 2 cut(s) 37, 215
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 1 cut(s) 257
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 69, 254, 334
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 1 cut(s) 292
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 4 cut(s) 33, 130, 144, 420
KasI GGCGCC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 312
KroI GCCGGC 2 cut(s) 68, 140
KroNI GCCGGC 2 cut(s) 70, 142
Kzo9I GATC 1 cut(s) 344
LmnI GCTCC 4 cut(s) 32, 151, 325, 427
Lsp1109I GCAGC 3 cut(s) 69, 72, 269
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 293, 349
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 4 cut(s) 21, 286, 389, 418
MfeI CAATTG 1 cut(s) 302
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 3 cut(s) 77, 229, 302
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 9 cut(s) 5, 61, 83, 116, 179, 188, 316, 421, 427
MroI TCCGGA 1 cut(s) 312
MroNI GCCGGC 2 cut(s) 68, 140
MspI CCGG 8 cut(s) 6, 30, 69, 114, 141, 249, 313, 425
MspR9I CCNGG 2 cut(s) 158, 262
MunI CAATTG 1 cut(s) 302
MvaI CCWGG 2 cut(s) 158, 262
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 4 cut(s) 44, 69, 254, 334
NaeI GCCGGC 2 cut(s) 70, 142
NarI GGCGCC 1 cut(s) 33
NdeII GATC 1 cut(s) 344
NgoMIV GCCGGC 2 cut(s) 68, 140
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 4 cut(s) 28, 34, 139, 346
NmeAIII GCCGAG 3 cut(s) 151, 211, 309
NmuCI GTSAC 2 cut(s) 293, 349
NspI RCATGY 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdiI GCCGGC 2 cut(s) 70, 142
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 7 cut(s) 37, 59, 62, 163, 184, 259, 368
PleI GAGTC 1 cut(s) 271
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 156, 260
PspGI CCWGG 2 cut(s) 156, 260
PspN4I GGNNCC 4 cut(s) 28, 34, 139, 346
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SacI GAGCTC 1 cut(s) 330
SatI GCNGC 7 cut(s) 36, 58, 61, 162, 183, 258, 367
Sau3AI GATC 1 cut(s) 344
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 158, 262
SduI GDGCHC 1 cut(s) 330
SetI ASST 6 cut(s) 53, 159, 180, 295, 330, 413
SfoI GGCGCC 1 cut(s) 34
Sse9I AATT 3 cut(s) 77, 229, 302
SsiI CCGC 5 cut(s) 36, 55, 162, 183, 366
SspDI GGCGCC 1 cut(s) 32
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 156, 260
TaaI ACNGT 1 cut(s) 225
TaiI ACGT 1 cut(s) 295
TaqI TCGA 1 cut(s) 171
TasI AATT 3 cut(s) 77, 229, 302
TauI GCSGC 4 cut(s) 38, 164, 185, 369
TfiI GAWTC 1 cut(s) 197
TseFI GTSAC 2 cut(s) 293, 349
TseI GCWGC 3 cut(s) 57, 60, 257
Tsp45I GTSAC 2 cut(s) 293, 349
TspGWI ACGGA 1 cut(s) 337
XapI RAATTY 1 cut(s) 229
XceI RCATGY 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.