pycom13g16170

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003646
Physical Location & Seq
Forward (+)
11912715 .. 11916179
3465 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGGGGAAGAAGAAGAAGAGAGCCGAATCAGAAGCAGAAGCACAACCAGAACAGAACAAAGTCGACGCTGTGAATGGCGACCCTCATCAGAAAGATAAGAAGAAGAAGAAGCACAAATTCGACCGACCCGCCGTGATACCTTCCGTGAGCGTAGCCGTCCCCGGTTCAATCATCGACAACACTCAATCCCTCGAACTCGCCACCCGATTGGCCAGTCAGATTGCTCGTGCAGCGACGATTTTCCGAATCGACGAGGTGGTGATATTTGACAATAAGAGTGAGTCTGGCTCATTGCCACAAGCTGATTCAGATGAGAATGAAAGCGGTGCTGCTTTTCTTGTACGAATCTTGAGGTACCTTGAGACACCTCAGTATTTGAGAAAAGCTCTATTTCCAAAACACAACAGCCTAAGATTTGTGGGCATGTTACCCCCGCTTGATGCTCCGCACCATCTGCGCAAACATGAATGGGGTCTGTTTCGGGAAGGTGTCACATTGAAAGAAAGACCTCCCAACCTTGTGGGAACACTAGTTGATGTGGGCTTGAGTAAGAATGTCATTGTTGATCAAGTACTTGATCCTGGAACAAGAGTTACTGTGGCTATGGGAGCCAGTTGGAACTTGGATGCTGGTAATGAATCTGTCCTGAATTTGTTTTCTGGCCTTTTCTGTTTTCTTTCTCAACCATTAATGGAGGCTTTACTATTGACTATGAATCTTATGTGGGTATTGGCTTCTTTCTATTGCATTGATCTAGATATATCACGCCAGGTTGTCTCATCCTCCAAGCCTAGGGAAGAAGCAGGAACATATTGGGGGTACAAAGTGCGCTATGCTTCTAATATTACTTCAGTCATGAATGAATGCCCATACAAGGGTGGCTATGACCATTCAATTGGTACCTCAGAGCACGGTCAGATTATTAATTCCTCTGAGCTCACTGTACCTACTTTCAGGCATCTATTGATTGCTTTCGGTGGACTTGCTGGGTTGGAAGAGAGCGTTGAAGAGGATGCTAACTTAAAGCCGAGCATTGAAGAGAATTTAGCATCAAAGTTCACTAATTGGAGGACATTAGGAACTTCCACAGTTGCCAAGATCACTGGGAATCCGAAATCAAGTCATGATTCTTCCGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

381

Amino Acids

41.89

Weight (kDa)

6.39

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 49 - 359 8.1e-91 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 458
Acc65I GGTACC 2 cut(s) 354, 899
AccB1I GGYRCC 2 cut(s) 354, 899
AccI GTMKAC 1 cut(s) 63
AciI CCGC 4 cut(s) 129, 324, 434, 446
AclWI GGATC 1 cut(s) 572
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 3 cut(s) 116, 649, 1042
AcuI CTGAAG 1 cut(s) 834
AfaI GTAC 6 cut(s) 342, 356, 573, 821, 901, 945
AfiI CCNNNNNNNGG 3 cut(s) 792, 874, 875
AgsI TTSAA 5 cut(s) 168, 499, 894, 1007, 1037
AhlI ACTAGT 1 cut(s) 529
AjnI CCWGG 2 cut(s) 580, 768
AluBI AGCT 3 cut(s) 302, 386, 937
AluI AGCT 3 cut(s) 302, 386, 937
Alw21I GWGCWC 2 cut(s) 912, 939
Alw26I GTCTC 2 cut(s) 356, 781
AlwI GGATC 1 cut(s) 572
AoxI GGCC 2 cut(s) 210, 661
ApeKI GCWGC 2 cut(s) 230, 329
ApoI RAATTY 3 cut(s) 116, 649, 1042
AseI ATTAAT 2 cut(s) 689, 924
Asp718I GGTACC 2 cut(s) 354, 899
AspA2I CCTAGG 1 cut(s) 791
AspLEI GCGC 2 cut(s) 459, 831
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 271
AvrII CCTAGG 1 cut(s) 791
BalI TGGCCA 1 cut(s) 212
BanI GGYRCC 2 cut(s) 354, 899
BanII GRGCYC 1 cut(s) 939
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 912, 939
BbvI GCAGC 2 cut(s) 242, 316
BccI CCATC 1 cut(s) 459
BceAI ACGGC 2 cut(s) 116, 140
BciT130I CCWGG 2 cut(s) 582, 770
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 356, 781
BcuI ACTAGT 1 cut(s) 529
BfaI CTAG 3 cut(s) 530, 755, 792
BisI GCNGC 2 cut(s) 231, 330
BlnI CCTAGG 1 cut(s) 791
BlsI GCNGC 2 cut(s) 232, 331
BmcAI AGTACT 1 cut(s) 573
Bme1390I CCNGG 3 cut(s) 162, 582, 770
BmiI GGNNCC 3 cut(s) 356, 610, 901
BmrFI CCNGG 3 cut(s) 162, 582, 770
BmrI ACTGGG 1 cut(s) 1113
BmsI GCATC 5 cut(s) 430, 616, 967, 1003, 1058
BmuI ACTGGG 1 cut(s) 1113
BplI GAGNNNNNCTC 4 cut(s) 272, 304, 370, 402
BpuEI CTTGAG 3 cut(s) 370, 380, 565
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 160, 791
Bsc4I CCNNNNNNNGG 3 cut(s) 792, 874, 875
Bse1I ACTGG 3 cut(s) 213, 612, 1108
Bse3DI GCAATG 1 cut(s) 290
BseBI CCWGG 2 cut(s) 582, 770
BseDI CCNNGG 2 cut(s) 160, 791
BseGI GGATG 3 cut(s) 631, 779, 1018
BseLI CCNNNNNNNGG 3 cut(s) 792, 874, 875
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 3 cut(s) 383, 918, 924
BseNI ACTGG 3 cut(s) 213, 612, 1108
BseXI GCAGC 2 cut(s) 242, 316
BseYI CCCAGC 1 cut(s) 986
BsgI GTGCAG 1 cut(s) 249
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 2 cut(s) 212, 663
BshNI GGYRCC 2 cut(s) 354, 899
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 912, 939
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 3 cut(s) 792, 874, 875
BsmAI GTCTC 2 cut(s) 356, 781
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 869
BsnI GGCC 2 cut(s) 212, 663
Bsp1286I GDGCHC 2 cut(s) 912, 939
Bsp143I GATC 4 cut(s) 565, 577, 751, 1098
BspACI CCGC 4 cut(s) 129, 324, 434, 446
BspANI GGCC 2 cut(s) 212, 663
BspCNI CTCAG 3 cut(s) 382, 917, 925
BspHI TCATGA 2 cut(s) 855, 1123
BspLI GGNNCC 3 cut(s) 356, 610, 901
BspPI GGATC 1 cut(s) 572
BspT107I GGYRCC 2 cut(s) 354, 899
BsrDI GCAATG 1 cut(s) 290
BsrI ACTGG 3 cut(s) 213, 612, 1108
BssECI CCNNGG 2 cut(s) 160, 791
BssMI GATC 4 cut(s) 565, 577, 751, 1098
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 791
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 582, 770
Bst4CI ACNGT 4 cut(s) 598, 914, 943, 1090
Bst6I CTCTTC 4 cut(s) 11, 990, 1002, 1032
BstAPI GCANNNNNTGC 1 cut(s) 454
BstDEI CTNAG 4 cut(s) 369, 410, 904, 933
BstF5I GGATG 3 cut(s) 631, 779, 1018
BstHHI GCGC 2 cut(s) 459, 831
BstKTI GATC 4 cut(s) 568, 580, 754, 1101
BstMAI GTCTC 2 cut(s) 356, 781
BstMBI GATC 4 cut(s) 565, 577, 751, 1098
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 3 cut(s) 230, 454, 608
BstNI CCWGG 2 cut(s) 582, 770
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 3 cut(s) 160, 580, 768
BstV1I GCAGC 2 cut(s) 242, 316
BstXI CCANNNNNNTGG 3 cut(s) 208, 520, 896
BsuRI GGCC 2 cut(s) 212, 663
BtsCI GGATG 3 cut(s) 631, 779, 1018
BtsIMutI CAGTG 2 cut(s) 939, 1101
CciI TCATGA 2 cut(s) 855, 1123
CfoI GCGC 2 cut(s) 459, 831
CseI GACGC 1 cut(s) 74
Csp6I GTAC 6 cut(s) 341, 355, 572, 820, 900, 944
CviAII CATG 4 cut(s) 424, 464, 856, 1124
CviQI GTAC 6 cut(s) 341, 355, 572, 820, 900, 944
DdeI CTNAG 4 cut(s) 369, 410, 904, 933
DpnI GATC 4 cut(s) 567, 579, 753, 1100
DpnII GATC 4 cut(s) 565, 577, 751, 1098
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 4 cut(s) 11, 990, 1002, 1032
EarI CTCTTC 4 cut(s) 11, 990, 1002, 1032
Ecl136II GAGCTC 1 cut(s) 937
Eco130I CCWWGG 1 cut(s) 791
Eco24I GRGCYC 1 cut(s) 939
Eco53kI GAGCTC 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 834
EcoICRI GAGCTC 1 cut(s) 937
EcoRII CCWGG 2 cut(s) 580, 768
EcoT14I CCWWGG 1 cut(s) 791
EcoT38I GRGCYC 1 cut(s) 939
ErhI CCWWGG 1 cut(s) 791
FaeI CATG 4 cut(s) 427, 467, 859, 1127
FaqI GGGAC 1 cut(s) 143
FatI CATG 4 cut(s) 423, 463, 855, 1123
FauI CCCGC 2 cut(s) 136, 441
FbaI TGATCA 1 cut(s) 565
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 231, 330
FokI GGATG 3 cut(s) 638, 766, 1025
FriOI GRGCYC 1 cut(s) 939
Fsp4HI GCNGC 2 cut(s) 231, 330
FspBI CTAG 3 cut(s) 530, 755, 792
FspI TGCGCA 1 cut(s) 458
GlaI GCGC 2 cut(s) 458, 830
GluI GCNGC 2 cut(s) 231, 330
GsaI CCCAGC 1 cut(s) 990
HaeIII GGCC 2 cut(s) 212, 663
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 74
HhaI GCGC 2 cut(s) 459, 831
Hin1II CATG 4 cut(s) 427, 467, 859, 1127
Hin6I GCGC 2 cut(s) 457, 829
HinP1I GCGC 2 cut(s) 457, 829
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 3 cut(s) 64, 980, 1059
Hpy188III TCNNGA 6 cut(s) 349, 482, 646, 755, 856, 1124
Hpy8I GTNNAC 3 cut(s) 64, 980, 1059
Hpy99I CGWCG 3 cut(s) 68, 238, 254
HpyAV CCTTC 2 cut(s) 150, 479
HpyCH4III ACNGT 4 cut(s) 598, 914, 943, 1090
HpyCH4V TGCA 2 cut(s) 230, 747
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 454, 608
HpyF3I CTNAG 4 cut(s) 369, 410, 904, 933
Hsp92II CATG 4 cut(s) 427, 467, 859, 1127
HspAI GCGC 2 cut(s) 457, 829
KpnI GGTACC 2 cut(s) 358, 903
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 4 cut(s) 565, 577, 751, 1098
LmnI GCTCC 2 cut(s) 448, 608
Lsp1109I GCAGC 2 cut(s) 242, 316
LweI GCATC 5 cut(s) 430, 616, 967, 1003, 1058
MaeI CTAG 3 cut(s) 530, 755, 792
MaeIII GTNAC 3 cut(s) 426, 490, 592
MalI GATC 4 cut(s) 567, 579, 753, 1100
MboI GATC 4 cut(s) 565, 577, 751, 1098
MfeI CAATTG 1 cut(s) 894
MhlI GDGCHC 2 cut(s) 912, 939
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 6 cut(s) 116, 649, 894, 925, 1042, 1063
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 2 cut(s) 596, 972
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
MseI TTAA 3 cut(s) 689, 924, 1022
Msp20I TGGCCA 1 cut(s) 212
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 3 cut(s) 162, 582, 770
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 1 cut(s) 869
MvaI CCWGG 2 cut(s) 582, 770
MwoI GCNNNNNNNGC 3 cut(s) 230, 454, 608
NciI CCSGG 1 cut(s) 162
NdeII GATC 4 cut(s) 565, 577, 751, 1098
NlaIII CATG 4 cut(s) 427, 467, 859, 1127
NlaIV GGNNCC 3 cut(s) 356, 610, 901
NmeAIII GCCGAG 1 cut(s) 1053
NmuCI GTSAC 1 cut(s) 490
NsbI TGCGCA 1 cut(s) 458
NspI RCATGY 1 cut(s) 427
PagI TCATGA 2 cut(s) 855, 1123
PctI GAATGC 1 cut(s) 869
PfeI GAWTC 9 cut(s) 26, 246, 305, 345, 638, 715, 1108, 1127, 1136
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 2 cut(s) 232, 331
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PshBI ATTAAT 2 cut(s) 689, 924
Psp124BI GAGCTC 1 cut(s) 939
Psp6I CCWGG 2 cut(s) 580, 768
PspFI CCCAGC 1 cut(s) 986
PspGI CCWGG 2 cut(s) 580, 768
PspN4I GGNNCC 3 cut(s) 356, 610, 901
PsrI GAACNNNNNNTAC 2 cut(s) 577, 609
RsaI GTAC 6 cut(s) 342, 356, 573, 821, 901, 945
RsaNI GTAC 6 cut(s) 341, 355, 572, 820, 900, 944
SacI GAGCTC 1 cut(s) 939
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 3 cut(s) 689, 924, 1022
SatI GCNGC 2 cut(s) 231, 330
Sau3AI GATC 4 cut(s) 565, 577, 751, 1098
ScaI AGTACT 1 cut(s) 573
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 162, 582, 770
SduI GDGCHC 2 cut(s) 912, 939
SfaNI GCATC 5 cut(s) 430, 616, 967, 1003, 1058
SmlI CTYRAG 3 cut(s) 349, 359, 544
SmoI CTYRAG 3 cut(s) 349, 359, 544
SpeI ACTAGT 1 cut(s) 529
Sse9I AATT 6 cut(s) 116, 649, 894, 925, 1042, 1063
SsiI CCGC 4 cut(s) 129, 324, 434, 446
SspI AATATT 1 cut(s) 844
SspMI CTAG 3 cut(s) 530, 755, 792
SstI GAGCTC 1 cut(s) 939
StyD4I CCNGG 3 cut(s) 160, 580, 768
StyI CCWWGG 1 cut(s) 791
TaaI ACNGT 4 cut(s) 598, 914, 943, 1090
TaqI TCGA 5 cut(s) 63, 120, 174, 192, 249
TaqII GACCGA 1 cut(s) 138
TasI AATT 6 cut(s) 116, 649, 894, 925, 1042, 1063
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 9 cut(s) 26, 246, 305, 345, 638, 715, 1108, 1127, 1136
Tru1I TTAA 3 cut(s) 689, 924, 1022
Tru9I TTAA 3 cut(s) 689, 924, 1022
TscAI CASTG 2 cut(s) 946, 1108
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 2 cut(s) 230, 329
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 7 cut(s) 333, 480, 651, 728, 872, 876, 1128
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 2 cut(s) 946, 1108
VspI ATTAAT 2 cut(s) 689, 924
XapI RAATTY 3 cut(s) 116, 649, 1042
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmaJI CCTAGG 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 63
XspI CTAG 3 cut(s) 530, 755, 792
ZrmI AGTACT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.