pycom15g07740

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002224
Physical Location & Seq
Forward (+)
4785532 .. 4788845
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g07740.1

Sequence Viewer

Length: 1422 bp
ATGGATGCAATTTTGACCTACGTTTTGATCTTGCTCTTCACCCTTCTGTCATTTGTAATTTTTATGAAAAGGGACAAGAGAGGAGGACAAGTACCCCAGAAAGGGGCTCAGCTCCCTCCAGGTTCATTAGGTTGGCCTTATATAGGAGAGACCCTCCAGCTCTATTCTCAAAACCCAAACACCTTCTTTTCTTCCAAACAGAAAAGGTACGGAGAGATTTTCAAAACGCATATCCTGGGATGTCCAAGTGTCATGCTGGCAAGCCCCGAGGCCGCAAAGTTTGTATTGGTGACTCGAGCTCACTTGTTCAAGCCCACGTATCCCAAAAGCAAAGAGCGTTTGATTGGTCCGTCAGCGCTGTTTTTTCACCAAGGAGATTACCATATGCGGATGAGGAAATTAGTTCAAGGATCTCTCTCCCTTGACATTATTCGAAATTTGGTGCCTCATATTGAAGGCTTAGCTGTTTCTGCCACAGATTCCTGGGCCACTGGCCAAGTCATCGACACCTTCCATCATATGAAGAAGTTATCTTTCGAGGTTGGTATACTTGCAATTCTTGGCAATTTGGAGGCACATTACAAAGAAGAGCTCAAAAAGAATTACACCATTGTGGACAAAGGCTACAATTCATTCCCCACAAACTTTCCGGGCACACCATACAAAAAGGCTTTATTGGCAAGGAAGAGGCTAAGAGTGATTTTGGGTGACATTATATGTGATAGGAAGGAGAAGAGATTACTCGAACAAGATCTATTAGGTTGCATGTTGAACCCAAAAGATGAAAAGGTTGAAGTCATAACTGATGACCAAATTGCTGACAACATTTTGGGTGTGCTTTTTGCTGCTCAAGACACCACAGCTAGTGTAATGACCTGGATTGTCAAGTACCTCCACGATGACCCTAAGCTTCTAGAGGCTGTAAAGGCTGAGCAGAAGATAATTCGTGAAGCAAATGAAGAAGGCCAGCACCCATTGAGCTGGGCACAGACTAGAAACATGCCAGTTAGTCACAAGGTTATTTTAGAGAGTTTGAGAATGGCAACTATTATATCTTTTGCCTTTAGAGAGGCTGTCGTTGATGTGGAATACAAAGGGTACTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTCAGGAACATTCATCACAATCCTGAATATTTTGCCGACCCTCTGAAATTCGATCTTTCTAGATTCGAGGTGGCACCAAAGCCAAATACATTTATGCCATTTGGAAGTGGAGTGCATGCCTGCCCCGGAAACGAGCTTGCAAAGCTGGAAATGCTGGTTATGATGCACTATTTGGTCACCAAGTTGAAGTGGGAAGTAGTGGATCGATCCCAAAGTGAGATTCAATATAGCCCCTTCCCTGTGCCTCTGCATGGACTCCCAACCAAATTTTGGAAACAATCTGCCTGA

Protein Analysis

474

Amino Acids

53.87

Weight (kDa)

9.2

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 455 2.7e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 442, 1207
AccB7I CCANNNNNTGG 1 cut(s) 1404
AccI GTMKAC 1 cut(s) 547
AciI CCGC 2 cut(s) 273, 388
AclWI GGATC 3 cut(s) 418, 1335, 1344
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 3 cut(s) 436, 1181, 1400
AfaI GTAC 4 cut(s) 93, 209, 890, 1100
AfeI AGCGCT 1 cut(s) 357
AfiI CCNNNNNNNGG 6 cut(s) 101, 102, 103, 143, 1385, 1404
AgsI TTSAA 8 cut(s) 223, 310, 407, 455, 772, 794, 1321, 1358
AjnI CCWGG 4 cut(s) 118, 234, 482, 875
AjuI GAANNNNNNNTTGG 2 cut(s) 1338, 1370
AleI CACNNNNGTG 1 cut(s) 611
Alw21I GWGCWC 2 cut(s) 301, 594
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 3 cut(s) 418, 1335, 1344
Ama87I CYCGRG 2 cut(s) 266, 294
Aor51HI AGCGCT 1 cut(s) 357
AoxI GGCC 5 cut(s) 134, 270, 486, 493, 964
ApeKI GCWGC 1 cut(s) 845
ApoI RAATTY 3 cut(s) 436, 1181, 1400
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 347, 486
AsuC2I CCSGG 2 cut(s) 651, 1260
AsuHPI GGTGA 5 cut(s) 31, 301, 359, 719, 1303
AsuII TTCGAA 1 cut(s) 433
AvaI CYCGRG 2 cut(s) 266, 294
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 2 cut(s) 656, 988
BalI TGGCCA 1 cut(s) 495
BanI GGYRCC 2 cut(s) 442, 1207
BanII GRGCYC 3 cut(s) 109, 301, 594
Bbv12I GWGCWC 2 cut(s) 301, 594
BbvI GCAGC 1 cut(s) 832
BccI CCATC 1 cut(s) 522
BciT130I CCWGG 4 cut(s) 120, 236, 484, 877
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 2 cut(s) 651, 1260
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 4 cut(s) 864, 914, 993, 1194
BfoI RGCGCY 1 cut(s) 359
BfuI GTATCC 1 cut(s) 330
BglII AGATCT 1 cut(s) 751
BisI GCNGC 2 cut(s) 273, 846
BlpI GCTNAGC 3 cut(s) 108, 460, 930
BlsI GCNGC 2 cut(s) 274, 847
Bme1390I CCNGG 6 cut(s) 120, 236, 484, 651, 877, 1260
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 2 cut(s) 266, 294
BmgT120I GGNCC 2 cut(s) 347, 486
BmiI GGNNCC 2 cut(s) 444, 1209
BmrFI CCNGG 6 cut(s) 120, 236, 484, 651, 877, 1260
BmsI GCATC 2 cut(s) 1116, 1287
BplI GAGNNNNNCTC 2 cut(s) 138, 170
BpmI CTGGAG 2 cut(s) 102, 140
Bpu10I CCTNAGC 1 cut(s) 906
Bpu1102I GCTNAGC 3 cut(s) 108, 460, 930
Bpu14I TTCGAA 1 cut(s) 433
BpuEI CTTGAG 1 cut(s) 834
BpuMI CCSGG 2 cut(s) 651, 1260
Bsa29I ATCGAT 1 cut(s) 1339
BsaAI YACGTR 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 5 cut(s) 235, 267, 370, 483, 1258
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 6 cut(s) 101, 102, 103, 143, 1385, 1404
Bse1I ACTGG 2 cut(s) 496, 1004
BseBI CCWGG 4 cut(s) 120, 236, 484, 877
BseCI ATCGAT 1 cut(s) 1339
BseDI CCNNGG 5 cut(s) 235, 267, 370, 483, 1258
BseGI GGATG 3 cut(s) 10, 245, 396
BseLI CCNNNNNNNGG 6 cut(s) 101, 102, 103, 143, 1385, 1404
BseMII CTCAG 2 cut(s) 122, 921
BseNI ACTGG 2 cut(s) 496, 1004
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 2 cut(s) 656, 988
BseXI GCAGC 1 cut(s) 832
BseYI CCCAGC 1 cut(s) 981
BshFI GGCC 5 cut(s) 136, 272, 488, 495, 966
BshNI GGYRCC 2 cut(s) 442, 1207
BshVI ATCGAT 1 cut(s) 1339
BsiHKAI GWGCWC 2 cut(s) 301, 594
BsiHKCI CYCGRG 2 cut(s) 266, 294
BsiSI CCGG 2 cut(s) 650, 1260
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 6 cut(s) 101, 102, 103, 143, 1385, 1404
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 5 cut(s) 136, 272, 488, 495, 966
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 2 cut(s) 266, 294
Bsp119I TTCGAA 1 cut(s) 433
Bsp1286I GDGCHC 5 cut(s) 109, 301, 594, 656, 988
Bsp143I GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
Bsp1720I GCTNAGC 3 cut(s) 108, 460, 930
BspACI CCGC 2 cut(s) 273, 388
BspANI GGCC 5 cut(s) 136, 272, 488, 495, 966
BspCNI CTCAG 2 cut(s) 121, 922
BspDI ATCGAT 1 cut(s) 1339
BspLI GGNNCC 2 cut(s) 444, 1209
BspPI GGATC 3 cut(s) 418, 1335, 1344
BspQI GCTCTTC 2 cut(s) 41, 582
BspT104I TTCGAA 1 cut(s) 433
BspT107I GGYRCC 2 cut(s) 442, 1207
BspTNI GGTCTC 1 cut(s) 143
BsrI ACTGG 2 cut(s) 496, 1004
BssECI CCNNGG 5 cut(s) 235, 267, 370, 483, 1258
BssMI GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
BssNAI GTATAC 1 cut(s) 548
BssT1I CCWWGG 1 cut(s) 370
Bst1107I GTATAC 1 cut(s) 548
Bst2UI CCWGG 4 cut(s) 120, 236, 484, 877
Bst6I CTCTTC 4 cut(s) 41, 582, 680, 728
BstBAI YACGTR 1 cut(s) 318
BstBI TTCGAA 1 cut(s) 433
BstC8I GCNNGC 6 cut(s) 258, 262, 968, 1251, 1255, 1272
BstDEI CTNAG 5 cut(s) 108, 460, 692, 906, 930
BstEII GGTNACC 1 cut(s) 1309
BstENI CCTNNNNNAGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 10, 245, 396
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 6 cut(s) 30, 413, 754, 1189, 1339, 1343
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
BstMWI GCNNNNNNNGC 5 cut(s) 470, 677, 926, 1276, 1285
BstNI CCWGG 4 cut(s) 120, 236, 484, 877
BstNSI RCATGY 3 cut(s) 769, 1003, 1253
BstPI GGTNACC 1 cut(s) 1309
BstSCI CCNGG 6 cut(s) 118, 234, 482, 649, 875, 1258
BstSLI GKGCMC 2 cut(s) 656, 988
BstV1I GCAGC 1 cut(s) 832
BstX2I RGATCY 2 cut(s) 410, 751
BstXI CCANNNNNNTGG 2 cut(s) 981, 1116
BstYI RGATCY 2 cut(s) 410, 751
BstZ17I GTATAC 1 cut(s) 548
Bsu15I ATCGAT 1 cut(s) 1339
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 5 cut(s) 136, 272, 488, 495, 966
BsuTUI ATCGAT 1 cut(s) 1339
BtsCI GGATG 3 cut(s) 10, 245, 396
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 6 cut(s) 258, 262, 968, 1251, 1255, 1272
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 347, 486
ClaI ATCGAT 1 cut(s) 1339
Csp6I GTAC 4 cut(s) 92, 208, 889, 1099
CviAII CATG 5 cut(s) 253, 766, 1000, 1250, 1385
CviQI GTAC 4 cut(s) 92, 208, 889, 1099
DdeI CTNAG 5 cut(s) 108, 460, 692, 906, 930
DpnI GATC 6 cut(s) 29, 412, 753, 1188, 1338, 1342
DpnII GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 4 cut(s) 41, 582, 680, 728
EarI CTCTTC 4 cut(s) 41, 582, 680, 728
Ecl136II GAGCTC 2 cut(s) 299, 592
Eco130I CCWWGG 1 cut(s) 370
Eco24I GRGCYC 3 cut(s) 109, 301, 594
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 347
Eco47III AGCGCT 1 cut(s) 357
Eco53kI GAGCTC 2 cut(s) 299, 592
Eco88I CYCGRG 2 cut(s) 266, 294
Eco91I GGTNACC 1 cut(s) 1309
EcoICRI GAGCTC 2 cut(s) 299, 592
EcoNI CCTNNNNNAGG 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 1309
EcoRII CCWGG 4 cut(s) 118, 234, 482, 875
EcoT14I CCWWGG 1 cut(s) 370
EcoT38I GRGCYC 3 cut(s) 109, 301, 594
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 5 cut(s) 256, 769, 1003, 1253, 1388
FaqI GGGAC 1 cut(s) 86
FatI CATG 5 cut(s) 252, 765, 999, 1249, 1384
FauNDI CATATG 2 cut(s) 384, 519
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 273, 846
FokI GGATG 3 cut(s) 17, 252, 403
FriOI GRGCYC 3 cut(s) 109, 301, 594
Fsp4HI GCNGC 2 cut(s) 273, 846
FspBI CTAG 4 cut(s) 864, 914, 993, 1194
GlaI GCGC 1 cut(s) 357
GluI GCNGC 2 cut(s) 273, 846
GsaI CCCAGC 1 cut(s) 985
GsuI CTGGAG 2 cut(s) 102, 140
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 5 cut(s) 136, 272, 488, 495, 966
HapII CCGG 2 cut(s) 650, 1260
HhaI GCGC 1 cut(s) 358
Hin1II CATG 5 cut(s) 256, 769, 1003, 1253, 1388
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 6 cut(s) 292, 479, 1105, 1197, 1354, 1389
HpaII CCGG 2 cut(s) 650, 1260
HphI GGTGA 5 cut(s) 31, 301, 359, 719, 1303
Hpy166II GTNNAC 2 cut(s) 548, 616
Hpy188I TCNGA 1 cut(s) 1179
Hpy188III TCNNGA 6 cut(s) 851, 914, 947, 1138, 1157, 1194
Hpy8I GTNNAC 2 cut(s) 548, 616
HpyAV CCTTC 7 cut(s) 53, 193, 449, 520, 721, 956, 1378
HpyCH4IV ACGT 2 cut(s) 21, 317
HpyCH4V TGCA 7 cut(s) 8, 554, 765, 1249, 1274, 1300, 1384
HpyF10VI GCNNNNNNNGC 5 cut(s) 470, 677, 926, 1276, 1285
HpyF3I CTNAG 5 cut(s) 108, 460, 692, 906, 930
HpySE526I ACGT 2 cut(s) 21, 317
Hsp92II CATG 5 cut(s) 256, 769, 1003, 1253, 1388
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
LguI GCTCTTC 2 cut(s) 41, 582
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 1 cut(s) 832
LweI GCATC 2 cut(s) 1116, 1287
MaeI CTAG 4 cut(s) 864, 914, 993, 1194
MaeII ACGT 2 cut(s) 21, 317
MaeIII GTNAC 4 cut(s) 289, 707, 1010, 1309
MalI GATC 6 cut(s) 29, 412, 753, 1188, 1338, 1342
MboI GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
MboII GAAGA 8 cut(s) 28, 183, 535, 599, 697, 745, 949, 971
MflI RGATCY 2 cut(s) 410, 751
MhlI GDGCHC 5 cut(s) 109, 301, 594, 656, 988
MlsI TGGCCA 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 2 cut(s) 286, 1383
MmeI TCCRAC 1 cut(s) 1097
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MslI CAYNNNNRTG 1 cut(s) 611
Msp20I TGGCCA 1 cut(s) 495
MspI CCGG 2 cut(s) 650, 1260
MspR9I CCNGG 6 cut(s) 120, 236, 484, 651, 877, 1260
MvaI CCWGG 4 cut(s) 120, 236, 484, 877
MwoI GCNNNNNNNGC 5 cut(s) 470, 677, 926, 1276, 1285
NciI CCSGG 2 cut(s) 651, 1260
NdeI CATATG 2 cut(s) 384, 519
NdeII GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
NlaIII CATG 5 cut(s) 256, 769, 1003, 1253, 1388
NlaIV GGNNCC 2 cut(s) 444, 1209
NmuCI GTSAC 4 cut(s) 289, 707, 1010, 1309
NspI RCATGY 3 cut(s) 769, 1003, 1253
NspV TTCGAA 1 cut(s) 433
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 1 cut(s) 1253
PaeR7I CTCGAG 1 cut(s) 294
PciSI GCTCTTC 2 cut(s) 41, 582
PfeI GAWTC 4 cut(s) 479, 1105, 1197, 1354
PflMI CCANNNNNTGG 1 cut(s) 1404
PkrI GCNGC 2 cut(s) 274, 847
PleI GAGTC 2 cut(s) 286, 1383
PpsI GAGTC 2 cut(s) 286, 1383
Ppu21I YACGTR 1 cut(s) 318
Psp124BI GAGCTC 2 cut(s) 301, 594
Psp6I CCWGG 4 cut(s) 118, 234, 482, 875
PspEI GGTNACC 1 cut(s) 1309
PspFI CCCAGC 1 cut(s) 981
PspGI CCWGG 4 cut(s) 118, 234, 482, 875
PspN4I GGNNCC 2 cut(s) 444, 1209
PspPI GGNCC 2 cut(s) 347, 486
PspXI VCTCGAGB 1 cut(s) 294
PsuI RGATCY 2 cut(s) 410, 751
RsaI GTAC 4 cut(s) 93, 209, 890, 1100
RsaNI GTAC 4 cut(s) 92, 208, 889, 1099
RseI CAYNNNNRTG 1 cut(s) 611
SacI GAGCTC 2 cut(s) 301, 594
SapI GCTCTTC 2 cut(s) 41, 582
SatI GCNGC 2 cut(s) 273, 846
Sau3AI GATC 6 cut(s) 27, 410, 751, 1186, 1336, 1340
Sau96I GGNCC 2 cut(s) 347, 486
SchI GAGTC 2 cut(s) 286, 1383
ScrFI CCNGG 6 cut(s) 120, 236, 484, 651, 877, 1260
SduI GDGCHC 5 cut(s) 109, 301, 594, 656, 988
SfaNI GCATC 2 cut(s) 1116, 1287
Sfr274I CTCGAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 433
SinI GGWCC 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 2 cut(s) 294, 849
SmoI CTYRAG 2 cut(s) 294, 849
SphI GCATGC 1 cut(s) 1253
SsiI CCGC 2 cut(s) 273, 388
SspI AATATT 1 cut(s) 1163
SspMI CTAG 4 cut(s) 864, 914, 993, 1194
SstI GAGCTC 2 cut(s) 301, 594
StyD4I CCNGG 6 cut(s) 118, 234, 482, 649, 875, 1258
StyI CCWWGG 1 cut(s) 370
TaiI ACGT 2 cut(s) 24, 320
TaqI TCGA 8 cut(s) 295, 433, 504, 537, 744, 1185, 1200, 1339
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 4 cut(s) 479, 1105, 1197, 1354
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 4 cut(s) 289, 707, 1010, 1309
TseI GCWGC 1 cut(s) 845
Tsp45I GTSAC 4 cut(s) 289, 707, 1010, 1309
TspDTI ATGAA 7 cut(s) 80, 114, 536, 621, 798, 972, 1136
TspGWI ACGGA 2 cut(s) 225, 339
TspRI CASTG 1 cut(s) 496
Van91I CCANNNNNTGG 1 cut(s) 1404
VpaK11BI GGWCC 1 cut(s) 347
XagI CCTNNNNNAGG 1 cut(s) 141
XapI RAATTY 3 cut(s) 436, 1181, 1400
XbaI TCTAGA 2 cut(s) 913, 1193
XceI RCATGY 3 cut(s) 769, 1003, 1253
XcmI CCANNNNNNNNNTGG 1 cut(s) 1401
XhoI CTCGAG 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 4 cut(s) 864, 914, 993, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.