RchiOBHm_Chr2g0105591

Cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002224
Physical Location & Seq
Reverse (-)
16881767 .. 16884975
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47980

Sequence Viewer

Length: 1407 bp
ATGAATACTGTTCTTTTATACTGTTTTGTATTTGCTCTACTCTCTTTCATATTACTTGGAAAACAAACGAGAAACAAACCCCGAGCAAAGCTTCCTCCAGGTTCAATGGGATGGCCATATCTTGGAGAAACCCTGCAACTCTACTCTCAAAATCCAAATGTCTTCTTTGGCACTAGGCAGCAAAGGTATGGAGAAATTTTCAAGACCCATATTCTTGGGAGCCCTGGTGTGATGCTGTGTAGCCCAGAGGCTATCCGGTTTGTGTTGGTGACTCGGGCACATTTGTTTAAGCCAACATACCCTAAAAGCAAGGAAAAATTGAGTGGCCCCTCGGCACTTTTCTTCCACCAAGATGACTACCATACTCAAATGAGAAGATTAGTCCAAGAATCACTGTCCTTGGATGCTATCAAGAACTTAGTCCCGGATATTGAAGCCATTGCCATTTCTACCTTGGACTCATGGTCTGGAGGGCAAATTTTCACTTTTGATGTTGCTGCATTGTTTATCTTCGGTCGCTCGAACACTCACTACAAAGAACAGCTAAAGAAAAACTATTTCACTCTAGACAAAGGTTACAATTGTTTCCCTATAAACCTACCAGGCTCTTCATACAATAAGTCGGTCTTGGCAAGGAGAAGGCTAAGCTTAATTCTCGGTGAGATAATAAAGGAGAGAAAGGAAAATAAATTGGTCGAGAAAGATCTATTGGGAAGCTTATTGAAGTTCAAGGATGAACAGGGTCAAACCTTAACACACAACCAGATCCTTGATAACATTATTGGGGTATTGTTTGCTGCTCAGGACACCACAGCTACTCTCCTGACTTGGATTCTCAAATACATGCACGATTACTCTGAACTTCTAGAAGCTATTCAGGTAGAACAGAGGGCAATTTTTGCAGCAAATGAGGGTGGAAACCAGTCGCTCAGTTGGGCTCAAACTAGAAATATGCCCCCTACCAGTAGGGCTGTAAAGGAGAGTTTGAGGATGGCAAGCATAATTTCTTTCACCTTCAGAGAAGCCGTTGAAGACGTTGAATATAAAGGATACTTGATCCGAAAGGGATGGAAGGTACTGCCTTTGTTCCGGAACATTCATCACAATCCTGATTTTTTCAATGAACCCCATAATTTCAATCCATCAAGATTTGAGCTTGGAATAAGACCCAATGCGTTCGTGCCATTTGGCAATGGAGTTCATACTTGTCCTGGAAATGAAGTTGCCAAGACTGAGATACTTATTTTCATCTACCACCTCGTTAACAAGTTCAGGTGGGAAGCAGCGGCGTCCGATGGTGGGATTCATTATGACCCATTTCCAGTACCTCACCAAGGACTTCCGGCCAAATTTTGGAAAGAGTATTCCGGCAGCCAAGGAGCGCTGTATGATAAAATAGCAAACTAG

Protein Analysis

468

Amino Acids

53.32

Weight (kDa)

9.2

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 32 - 428 2e-62 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 122, 1353
AccIII TCCGGA 1 cut(s) 1089
AciI CCGC 1 cut(s) 1286
AclWI GGATC 2 cut(s) 760, 1051
AcoI YGGCCR 2 cut(s) 113, 1344
AcsI RAATTY 3 cut(s) 195, 477, 1349
AcuI CTGAAG 1 cut(s) 1000
AcyI GRCGYC 1 cut(s) 1289
AfaI GTAC 2 cut(s) 1077, 1326
AfeI AGCGCT 1 cut(s) 1383
AfiI CCNNNNNNNGG 4 cut(s) 122, 1299, 1334, 1353
AgsI TTSAA 9 cut(s) 105, 202, 434, 724, 730, 1031, 1040, 1120, 1138
AhdI GACNNNNNGTC 1 cut(s) 463
AjnI CCWGG 4 cut(s) 97, 223, 601, 1210
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AluBI AGCT 7 cut(s) 91, 544, 648, 717, 815, 872, 1156
AluI AGCT 7 cut(s) 91, 544, 648, 717, 815, 872, 1156
AlwI GGATC 2 cut(s) 760, 1051
Ama87I CYCGRG 2 cut(s) 81, 273
Aor13HI TCCGGA 1 cut(s) 1089
Aor51HI AGCGCT 1 cut(s) 1383
AoxI GGCC 3 cut(s) 113, 325, 1344
ApeKI GCWGC 6 cut(s) 178, 497, 797, 902, 1283, 1371
ApoI RAATTY 3 cut(s) 195, 477, 1349
Asp700I GAANNNNTTC 1 cut(s) 873
AspLEI GCGC 1 cut(s) 1384
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 4 cut(s) 280, 671, 1003, 1322
AvaI CYCGRG 2 cut(s) 81, 273
BaeGI GKGCMC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 115
BanII GRGCYC 2 cut(s) 224, 940
BbsI GAAGAC 2 cut(s) 154, 1038
BbvI GCAGC 6 cut(s) 190, 484, 784, 914, 1295, 1383
BccI CCATC 5 cut(s) 105, 985, 1062, 1150, 1289
BceAI ACGGC 1 cut(s) 1010
BciT130I CCWGG 4 cut(s) 99, 225, 603, 1212
BciVI GTATCC 1 cut(s) 1043
BcnI CCSGG 1 cut(s) 425
BfaI CTAG 5 cut(s) 174, 566, 866, 945, 1405
BfoI RGCGCY 1 cut(s) 1385
BfuI GTATCC 1 cut(s) 1043
BglII AGATCT 1 cut(s) 703
BisI GCNGC 7 cut(s) 179, 498, 798, 903, 1284, 1287, 1372
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 7 cut(s) 180, 499, 799, 904, 1285, 1288, 1373
Bme1390I CCNGG 5 cut(s) 99, 225, 425, 603, 1212
BmeRI GACNNNNNGTC 1 cut(s) 463
BmeT110I CYCGRG 2 cut(s) 81, 273
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 221, 328
BmrFI CCNGG 5 cut(s) 99, 225, 425, 603, 1212
BmsI GCATC 2 cut(s) 222, 394
BpiI GAAGAC 2 cut(s) 154, 1038
BpmI CTGGAG 2 cut(s) 81, 489
Bpu10I CCTNAGC 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 644
BpuMI CCSGG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 1289
BsaJI CCNNGG 6 cut(s) 223, 330, 399, 453, 1333, 1375
BsaWI WCCGGW 2 cut(s) 255, 1089
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 1299, 1334, 1353
Bse1I ACTGG 3 cut(s) 922, 963, 1322
Bse3DI GCAATG 2 cut(s) 438, 1198
BseAI TCCGGA 1 cut(s) 1089
BseBI CCWGG 4 cut(s) 99, 225, 603, 1212
BseDI CCNNGG 6 cut(s) 223, 330, 399, 453, 1333, 1375
BseGI GGATG 5 cut(s) 116, 409, 739, 996, 1073
BseLI CCNNNNNNNGG 4 cut(s) 122, 1299, 1334, 1353
BseMI GCAATG 2 cut(s) 438, 1198
BseMII CTCAG 3 cut(s) 815, 943, 1224
BseNI ACTGG 3 cut(s) 922, 963, 1322
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 6 cut(s) 190, 484, 784, 914, 1295, 1383
Bsh1285I CGRYCG 1 cut(s) 517
BshFI GGCC 3 cut(s) 115, 327, 1346
BsiEI CGRYCG 1 cut(s) 517
BsiHKCI CYCGRG 2 cut(s) 81, 273
BsiSI CCGG 5 cut(s) 256, 425, 1090, 1343, 1368
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 4 cut(s) 122, 1299, 1334, 1353
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 3 cut(s) 115, 327, 1346
BsoBI CYCGRG 2 cut(s) 81, 273
Bsp1286I GDGCHC 3 cut(s) 224, 280, 940
Bsp13I TCCGGA 1 cut(s) 1089
Bsp143I GATC 3 cut(s) 703, 765, 1056
Bsp1720I GCTNAGC 1 cut(s) 644
BspACI CCGC 1 cut(s) 1286
BspANI GGCC 3 cut(s) 115, 327, 1346
BspCNI CTCAG 3 cut(s) 814, 942, 1225
BspEI TCCGGA 1 cut(s) 1089
BspLI GGNNCC 2 cut(s) 221, 328
BspPI GGATC 2 cut(s) 760, 1051
BspQI GCTCTTC 1 cut(s) 613
BsrDI GCAATG 2 cut(s) 438, 1198
BsrI ACTGG 3 cut(s) 922, 963, 1322
BssECI CCNNGG 6 cut(s) 223, 330, 399, 453, 1333, 1375
BssMI GATC 3 cut(s) 703, 765, 1056
BssNI GRCGYC 1 cut(s) 1289
BssT1I CCWWGG 4 cut(s) 399, 453, 1333, 1375
Bst2UI CCWGG 4 cut(s) 99, 225, 603, 1212
Bst4CI ACNGT 3 cut(s) 10, 23, 396
Bst6I CTCTTC 1 cut(s) 613
BstACI GRCGYC 1 cut(s) 1289
BstAPI GCANNNNNTGC 1 cut(s) 899
BstC8I GCNNGC 1 cut(s) 997
BstDEI CTNAG 5 cut(s) 418, 644, 801, 929, 1233
BstENI CCTNNNNNAGG 1 cut(s) 1332
BstF5I GGATG 5 cut(s) 116, 409, 739, 996, 1073
BstH2I RGCGCY 1 cut(s) 1385
BstHHI GCGC 1 cut(s) 1384
BstKTI GATC 3 cut(s) 706, 768, 1059
BstMBI GATC 3 cut(s) 703, 765, 1056
BstMCI CGRYCG 1 cut(s) 517
BstMWI GCNNNNNNNGC 1 cut(s) 899
BstNI CCWGG 4 cut(s) 99, 225, 603, 1212
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 5 cut(s) 97, 223, 423, 601, 1210
BstSLI GKGCMC 1 cut(s) 280
BstV1I GCAGC 6 cut(s) 190, 484, 784, 914, 1295, 1383
BstV2I GAAGAC 2 cut(s) 154, 1038
BstX2I RGATCY 2 cut(s) 703, 765
BstXI CCANNNNNNTGG 1 cut(s) 215
BstYI RGATCY 2 cut(s) 703, 765
BsuI GTATCC 1 cut(s) 1043
BsuRI GGCC 3 cut(s) 115, 327, 1346
BtsCI GGATG 5 cut(s) 116, 409, 739, 996, 1073
BtsIMutI CAGTG 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 997
CfoI GCGC 1 cut(s) 1384
Cfr13I GGNCC 1 cut(s) 326
CseI GACGC 1 cut(s) 1278
Csp6I GTAC 2 cut(s) 1076, 1325
CviAII CATG 2 cut(s) 462, 844
CviQI GTAC 2 cut(s) 1076, 1325
DdeI CTNAG 5 cut(s) 418, 644, 801, 929, 1233
DpnI GATC 3 cut(s) 705, 767, 1058
DpnII GATC 3 cut(s) 703, 765, 1056
DriI GACNNNNNGTC 1 cut(s) 463
EaeI YGGCCR 2 cut(s) 113, 1344
Eam1104I CTCTTC 1 cut(s) 613
Eam1105I GACNNNNNGTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 613
Eco130I CCWWGG 4 cut(s) 399, 453, 1333, 1375
Eco24I GRGCYC 2 cut(s) 224, 940
Eco47III AGCGCT 1 cut(s) 1383
Eco57I CTGAAG 1 cut(s) 1000
Eco88I CYCGRG 2 cut(s) 81, 273
EcoNI CCTNNNNNAGG 1 cut(s) 1332
EcoRII CCWGG 4 cut(s) 97, 223, 601, 1210
EcoT14I CCWWGG 4 cut(s) 399, 453, 1333, 1375
EcoT38I GRGCYC 2 cut(s) 224, 940
ErhI CCWWGG 4 cut(s) 399, 453, 1333, 1375
FaeI CATG 2 cut(s) 465, 847
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 407
FatI CATG 2 cut(s) 461, 843
Fnu4HI GCNGC 7 cut(s) 179, 498, 798, 903, 1284, 1287, 1372
FokI GGATG 5 cut(s) 123, 416, 746, 1003, 1080
FriOI GRGCYC 2 cut(s) 224, 940
Fsp4HI GCNGC 7 cut(s) 179, 498, 798, 903, 1284, 1287, 1372
FspBI CTAG 5 cut(s) 174, 566, 866, 945, 1405
GlaI GCGC 1 cut(s) 1383
GluI GCNGC 7 cut(s) 179, 498, 798, 903, 1284, 1287, 1372
GsuI CTGGAG 2 cut(s) 81, 489
HaeII RGCGCY 1 cut(s) 1385
HaeIII GGCC 3 cut(s) 115, 327, 1346
HapII CCGG 5 cut(s) 256, 425, 1090, 1343, 1368
HgaI GACGC 1 cut(s) 1278
HhaI GCGC 1 cut(s) 1384
Hin1I GRCGYC 1 cut(s) 1289
Hin1II CATG 2 cut(s) 465, 847
Hin6I GCGC 1 cut(s) 1382
HinP1I GCGC 1 cut(s) 1382
HincII GTYRAC 1 cut(s) 1264
HindII GTYRAC 1 cut(s) 1264
HindIII AAGCTT 3 cut(s) 89, 646, 715
HinfI GANTC 5 cut(s) 271, 389, 458, 832, 1303
HpaI GTTAAC 1 cut(s) 1264
HpaII CCGG 5 cut(s) 256, 425, 1090, 1343, 1368
HphI GGTGA 4 cut(s) 280, 671, 1003, 1322
Hpy166II GTNNAC 1 cut(s) 1264
Hpy188I TCNGA 4 cut(s) 859, 1019, 1061, 1294
Hpy8I GTNNAC 1 cut(s) 1264
HpyAV CCTTC 3 cut(s) 633, 1024, 1066
HpyCH4III ACNGT 3 cut(s) 10, 23, 396
HpyCH4IV ACGT 1 cut(s) 1035
HpyCH4V TGCA 4 cut(s) 136, 500, 847, 902
HpyF10VI GCNNNNNNNGC 1 cut(s) 899
HpyF3I CTNAG 5 cut(s) 418, 644, 801, 929, 1233
HpySE526I ACGT 1 cut(s) 1035
Hsp92I GRCGYC 1 cut(s) 1289
Hsp92II CATG 2 cut(s) 465, 847
HspAI GCGC 1 cut(s) 1382
Kpn2I TCCGGA 1 cut(s) 1089
KspAI GTTAAC 1 cut(s) 1264
Kzo9I GATC 3 cut(s) 703, 765, 1056
LguI GCTCTTC 1 cut(s) 613
LmnI GCTCC 2 cut(s) 219, 1379
Lsp1109I GCAGC 6 cut(s) 190, 484, 784, 914, 1295, 1383
LweI GCATC 2 cut(s) 222, 394
MaeI CTAG 5 cut(s) 174, 566, 866, 945, 1405
MaeII ACGT 1 cut(s) 1035
MaeIII GTNAC 2 cut(s) 268, 575
MalI GATC 3 cut(s) 705, 767, 1058
MboI GATC 3 cut(s) 703, 765, 1056
MboII GAAGA 6 cut(s) 154, 334, 387, 502, 600, 1043
MfeI CAATTG 1 cut(s) 580
MflI RGATCY 2 cut(s) 703, 765
MhlI GDGCHC 3 cut(s) 224, 280, 940
MlsI TGGCCA 1 cut(s) 115
MluNI TGGCCA 1 cut(s) 115
MlyI GAGTC 2 cut(s) 265, 452
MnlI CCTC 9 cut(s) 105, 241, 340, 464, 882, 904, 981, 1268, 1338
Mox20I TGGCCA 1 cut(s) 115
MroI TCCGGA 1 cut(s) 1089
MroXI GAANNNNTTC 1 cut(s) 873
MscI TGGCCA 1 cut(s) 115
MseI TTAA 4 cut(s) 288, 650, 752, 1263
MslI CAYNNNNRTG 1 cut(s) 351
Msp20I TGGCCA 1 cut(s) 115
MspA1I CMGCKG 1 cut(s) 1286
MspI CCGG 5 cut(s) 256, 425, 1090, 1343, 1368
MspR9I CCNGG 5 cut(s) 99, 225, 425, 603, 1212
MunI CAATTG 1 cut(s) 580
MvaI CCWGG 4 cut(s) 99, 225, 603, 1212
MwoI GCNNNNNNNGC 1 cut(s) 899
NciI CCSGG 1 cut(s) 425
NdeII GATC 3 cut(s) 703, 765, 1056
NlaIII CATG 2 cut(s) 465, 847
NlaIV GGNNCC 2 cut(s) 221, 328
NmeAIII GCCGAG 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 847
PciSI GCTCTTC 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 873
PfeI GAWTC 3 cut(s) 389, 832, 1303
PflMI CCANNNNNTGG 2 cut(s) 122, 1353
PfoI TCCNGGA 2 cut(s) 423, 1210
PkrI GCNGC 7 cut(s) 180, 499, 799, 904, 1285, 1288, 1373
PleI GAGTC 2 cut(s) 265, 452
PpsI GAGTC 2 cut(s) 265, 452
Psp6I CCWGG 4 cut(s) 97, 223, 601, 1210
PspGI CCWGG 4 cut(s) 97, 223, 601, 1210
PspN4I GGNNCC 2 cut(s) 221, 328
PspPI GGNCC 1 cut(s) 326
PsrI GAACNNNNNNTAC 2 cut(s) 515, 547
PsuI RGATCY 2 cut(s) 703, 765
RsaI GTAC 2 cut(s) 1077, 1326
RsaNI GTAC 2 cut(s) 1076, 1325
RseI CAYNNNNRTG 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 613
SaqAI TTAA 4 cut(s) 288, 650, 752, 1263
SatI GCNGC 7 cut(s) 179, 498, 798, 903, 1284, 1287, 1372
Sau3AI GATC 3 cut(s) 703, 765, 1056
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 2 cut(s) 265, 452
ScrFI CCNGG 5 cut(s) 99, 225, 425, 603, 1212
SduI GDGCHC 3 cut(s) 224, 280, 940
SfaNI GCATC 2 cut(s) 222, 394
SmiMI CAYNNNNRTG 1 cut(s) 351
SsiI CCGC 1 cut(s) 1286
SspMI CTAG 5 cut(s) 174, 566, 866, 945, 1405
StyD4I CCNGG 5 cut(s) 97, 223, 423, 601, 1210
StyI CCWWGG 4 cut(s) 399, 453, 1333, 1375
TaaI ACNGT 3 cut(s) 10, 23, 396
TaiI ACGT 1 cut(s) 1038
TaqI TCGA 2 cut(s) 521, 696
TaqII GACCGA 2 cut(s) 503, 613
TauI GCSGC 1 cut(s) 1289
TfiI GAWTC 3 cut(s) 389, 832, 1303
Tru1I TTAA 4 cut(s) 288, 650, 752, 1263
Tru9I TTAA 4 cut(s) 288, 650, 752, 1263
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 6 cut(s) 178, 497, 797, 902, 1283, 1371
Tsp45I GTSAC 1 cut(s) 268
TspRI CASTG 1 cut(s) 399
Van91I CCANNNNNTGG 2 cut(s) 122, 1353
XagI CCTNNNNNAGG 1 cut(s) 1332
XapI RAATTY 3 cut(s) 195, 477, 1349
XbaI TCTAGA 2 cut(s) 565, 865
XceI RCATGY 1 cut(s) 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 873
XspI CTAG 5 cut(s) 174, 566, 866, 945, 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.