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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 116, 5114 |
| Acc16I | TGCGCA | 1 cut(s) | 4083 |
| Acc36I | ACCTGC | 3 cut(s) | 2050, 2197, 4075 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 5480 |
| AccBSI | CCGCTC | 1 cut(s) | 2338 |
| AccII | CGCG | 1 cut(s) | 263 |
| AccIII | TCCGGA | 1 cut(s) | 1366 |
| AclWI | GGATC | 5 cut(s) | 1140, 1923, 2532, 3779, 6076 |
| AcoI | YGGCCR | 2 cut(s) | 3675, 4857 |
| AcuI | CTGAAG | 6 cut(s) | 216, 546, 1578, 2439, 4565, 5532 |
| AcyI | GRCGYC | 2 cut(s) | 113, 5111 |
| AfaI | GTAC | 5 cut(s) | 22, 2760, 4785, 5500, 5545 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1129, 2095, 2242, 4918, 5480 |
| AflII | CTTAAG | 2 cut(s) | 1930, 3214 |
| AflIII | ACRYGT | 3 cut(s) | 487, 1357, 2722 |
| AjiI | CACGTC | 1 cut(s) | 2723 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1930, 1962 |
| Alw26I | GTCTC | 9 cut(s) | 160, 216, 1241, 2824, 3507, 3916, 4336, 4373, 5037 |
| AlwI | GGATC | 5 cut(s) | 1140, 1923, 2532, 3779, 6076 |
| AlwNI | CAGNNNCTG | 6 cut(s) | 281, 1019, 4423, 4484, 5347, 5744 |
| Ama87I | CYCGRG | 2 cut(s) | 1829, 2267 |
| Aor13HI | TCCGGA | 1 cut(s) | 1366 |
| AoxI | GGCC | 5 cut(s) | 1119, 1160, 1621, 3675, 4857 |
| ApaI | GGGCCC | 1 cut(s) | 1123 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1324, 1356, 4560, 4592 |
| AseI | ATTAAT | 2 cut(s) | 627, 3291 |
| Asp700I | GAANNNNTTC | 8 cut(s) | 1095, 1398, 2089, 3372, 3777, 3978, 4107, 4233 |
| AspLEI | GCGC | 2 cut(s) | 2869, 4084 |
| AspS9I | GGNCC | 4 cut(s) | 1119, 1120, 1200, 4962 |
| AsuC2I | CCSGG | 1 cut(s) | 1286 |
| AsuNHI | GCTAGC | 1 cut(s) | 508 |
| AvaI | CYCGRG | 2 cut(s) | 1829, 2267 |
| AvaII | GGWCC | 2 cut(s) | 1200, 4962 |
| AxyI | CCTNAGG | 2 cut(s) | 1035, 4974 |
| BaeGI | GKGCMC | 2 cut(s) | 446, 1123 |
| BanII | GRGCYC | 1 cut(s) | 1123 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 5527, 5559 |
| BauI | CACGAG | 2 cut(s) | 2294, 4168 |
| BbsI | GAAGAC | 5 cut(s) | 3090, 4293, 4611, 5100, 5133 |
| BceAI | ACGGC | 2 cut(s) | 3, 1650 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1659, 1693 |
| BciVI | GTATCC | 1 cut(s) | 3511 |
| BclI | TGATCA | 3 cut(s) | 577, 3358, 5191 |
| BcnI | CCSGG | 1 cut(s) | 1286 |
| BcoDI | GTCTC | 9 cut(s) | 160, 216, 1241, 2824, 3507, 3916, 4336, 4373, 5037 |
| BfmI | CTRYAG | 8 cut(s) | 174, 273, 1899, 1910, 2019, 3741, 5246, 5945 |
| BfrI | CTTAAG | 2 cut(s) | 1930, 3214 |
| BfuAI | ACCTGC | 3 cut(s) | 2050, 2197, 4075 |
| BfuI | GTATCC | 1 cut(s) | 3511 |
| BglII | AGATCT | 5 cut(s) | 928, 1046, 3351, 3568, 5855 |
| BlpI | GCTNAGC | 2 cut(s) | 1268, 4319 |
| Bme18I | GGWCC | 2 cut(s) | 1200, 4962 |
| BmeT110I | CYCGRG | 2 cut(s) | 1829, 2267 |
| BmgBI | CACGTC | 1 cut(s) | 2723 |
| BmgT120I | GGNCC | 4 cut(s) | 1119, 1120, 1200, 4962 |
| BmiI | GGNNCC | 5 cut(s) | 312, 1120, 1121, 2608, 4971 |
| BmrI | ACTGGG | 1 cut(s) | 447 |
| BmtI | GCTAGC | 1 cut(s) | 512 |
| BmuI | ACTGGG | 1 cut(s) | 447 |
| BpiI | GAAGAC | 5 cut(s) | 3090, 4293, 4611, 5100, 5133 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 2250, 2282, 4353, 4385 |
| BpmI | CTGGAG | 7 cut(s) | 1149, 1571, 2814, 3848, 4746, 5021, 5117 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1268, 4319 |
| BpuMI | CCSGG | 1 cut(s) | 1286 |
| Bsa29I | ATCGAT | 1 cut(s) | 4656 |
| BsaBI | GATNNNNATC | 6 cut(s) | 225, 576, 1137, 1845, 3321, 4707 |
| BsaHI | GRCGYC | 2 cut(s) | 113, 5111 |
| BsaI | GGTCTC | 1 cut(s) | 4373 |
| BsaJI | CCNNGG | 7 cut(s) | 261, 303, 636, 1618, 4335, 4965, 5927 |
| BsaWI | WCCGGW | 2 cut(s) | 1366, 3436 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1129, 2095, 2242, 4918, 5480 |
| Bse118I | RCCGGY | 1 cut(s) | 3677 |
| Bse21I | CCTNAGG | 2 cut(s) | 1035, 4974 |
| Bse3DI | GCAATG | 5 cut(s) | 684, 4323, 5194, 5360, 5523 |
| Bse8I | GATNNNNATC | 6 cut(s) | 225, 576, 1137, 1845, 3321, 4707 |
| BseAI | TCCGGA | 1 cut(s) | 1366 |
| BseCI | ATCGAT | 1 cut(s) | 4656 |
| BseDI | CCNNGG | 7 cut(s) | 261, 303, 636, 1618, 4335, 4965, 5927 |
| BseGI | GGATG | 6 cut(s) | 632, 706, 1813, 2501, 2998, 6070 |
| BseJI | GATNNNNATC | 6 cut(s) | 225, 576, 1137, 1845, 3321, 4707 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1129, 2095, 2242, 4918, 5480 |
| BseMI | GCAATG | 5 cut(s) | 684, 4323, 5194, 5360, 5523 |
| BseSI | GKGCMC | 2 cut(s) | 446, 1123 |
| BsgI | GTGCAG | 5 cut(s) | 662, 1463, 1594, 3674, 5036 |
| Bsh1236I | CGCG | 1 cut(s) | 263 |
| BshFI | GGCC | 5 cut(s) | 1121, 1162, 1623, 3677, 4859 |
| BshVI | ATCGAT | 1 cut(s) | 4656 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1829, 2267 |
| BsiSI | CCGG | 5 cut(s) | 1285, 1367, 2921, 3437, 3678 |
| BslFI | GGGAC | 3 cut(s) | 1345, 2733, 4363 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1129, 2095, 2242, 4918, 5480 |
| BsmAI | GTCTC | 9 cut(s) | 160, 216, 1241, 2824, 3507, 3916, 4336, 4373, 5037 |
| BsmFI | GGGAC | 3 cut(s) | 1345, 2733, 4363 |
| BsnI | GGCC | 5 cut(s) | 1121, 1162, 1623, 3677, 4859 |
| Bso31I | GGTCTC | 1 cut(s) | 4373 |
| BsoBI | CYCGRG | 2 cut(s) | 1829, 2267 |
| Bsp120I | GGGCCC | 1 cut(s) | 1119 |
| Bsp1286I | GDGCHC | 2 cut(s) | 446, 1123 |
| Bsp13I | TCCGGA | 1 cut(s) | 1366 |
| Bsp1407I | TGTACA | 2 cut(s) | 2758, 4783 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1268, 4319 |
| Bsp19I | CCATGG | 1 cut(s) | 4965 |
| BspANI | GGCC | 5 cut(s) | 1121, 1162, 1623, 3677, 4859 |
| BspDI | ATCGAT | 1 cut(s) | 4656 |
| BspEI | TCCGGA | 1 cut(s) | 1366 |
| BspFNI | CGCG | 1 cut(s) | 263 |
| BspHI | TCATGA | 3 cut(s) | 1852, 2560, 5194 |
| BspLI | GGNNCC | 5 cut(s) | 312, 1120, 1121, 2608, 4971 |
| BspMAI | CTGCAG | 1 cut(s) | 5250 |
| BspMI | ACCTGC | 3 cut(s) | 2050, 2197, 4075 |
| BspOI | GCTAGC | 1 cut(s) | 512 |
| BspPI | GGATC | 5 cut(s) | 1140, 1923, 2532, 3779, 6076 |
| BspQI | GCTCTTC | 1 cut(s) | 1554 |
| BspTI | CTTAAG | 2 cut(s) | 1930, 3214 |
| BspTNI | GGTCTC | 1 cut(s) | 4373 |
| BsrBI | CCGCTC | 1 cut(s) | 2338 |
| BsrDI | GCAATG | 5 cut(s) | 684, 4323, 5194, 5360, 5523 |
| BsrFI | RCCGGY | 1 cut(s) | 3677 |
| BsrGI | TGTACA | 2 cut(s) | 2758, 4783 |
| BssAI | RCCGGY | 1 cut(s) | 3677 |
| BssECI | CCNNGG | 7 cut(s) | 261, 303, 636, 1618, 4335, 4965, 5927 |
| BssNI | GRCGYC | 2 cut(s) | 113, 5111 |
| BssSI | CACGAG | 2 cut(s) | 2294, 4168 |
| BssT1I | CCWWGG | 4 cut(s) | 636, 1618, 4335, 4965 |
| Bst2BI | CACGAG | 2 cut(s) | 2294, 4168 |
| Bst6I | CTCTTC | 9 cut(s) | 487, 1272, 1512, 1554, 1953, 2121, 2724, 4587, 4709 |
| BstACI | GRCGYC | 2 cut(s) | 113, 5111 |
| BstAFI | CTTAAG | 2 cut(s) | 1930, 3214 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1441, 5412, 5791, 6065 |
| BstAUI | TGTACA | 2 cut(s) | 2758, 4783 |
| BstC8I | GCNNGC | 7 cut(s) | 33, 510, 807, 1738, 1974, 4023, 4665 |
| BstDSI | CCRYGG | 2 cut(s) | 261, 4965 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1127 |
| BstF5I | GGATG | 6 cut(s) | 632, 706, 1813, 2501, 2998, 6070 |
| BstFNI | CGCG | 1 cut(s) | 263 |
| BstHHI | GCGC | 2 cut(s) | 2869, 4084 |
| BstMAI | GTCTC | 9 cut(s) | 160, 216, 1241, 2824, 3507, 3916, 4336, 4373, 5037 |
| BstNSI | RCATGY | 3 cut(s) | 491, 1361, 3998 |
| BstSFI | CTRYAG | 8 cut(s) | 174, 273, 1899, 1910, 2019, 3741, 5246, 5945 |
| BstSLI | GKGCMC | 2 cut(s) | 446, 1123 |
| BstUI | CGCG | 1 cut(s) | 263 |
| BstV2I | GAAGAC | 5 cut(s) | 3090, 4293, 4611, 5100, 5133 |
| BstX2I | RGATCY | 5 cut(s) | 928, 1046, 3351, 3568, 5855 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 424 |
| BstYI | RGATCY | 5 cut(s) | 928, 1046, 3351, 3568, 5855 |
| Bsu15I | ATCGAT | 1 cut(s) | 4656 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1035, 4974 |
| BsuI | GTATCC | 1 cut(s) | 3511 |
| BsuRI | GGCC | 5 cut(s) | 1121, 1162, 1623, 3677, 4859 |
| BsuTUI | ATCGAT | 1 cut(s) | 4656 |
| BtgI | CCRYGG | 2 cut(s) | 261, 4965 |
| BtgZI | GCGATG | 2 cut(s) | 4935, 5058 |
| BtrI | CACGTC | 1 cut(s) | 2723 |
| BtsCI | GGATG | 6 cut(s) | 632, 706, 1813, 2501, 2998, 6070 |
| BtsI | GCAGTG | 3 cut(s) | 94, 186, 2650 |
| BveI | ACCTGC | 3 cut(s) | 2050, 2197, 4075 |
| Cac8I | GCNNGC | 7 cut(s) | 33, 510, 807, 1738, 1974, 4023, 4665 |
| CaiI | CAGNNNCTG | 6 cut(s) | 281, 1019, 4423, 4484, 5347, 5744 |
| CciI | TCATGA | 3 cut(s) | 1852, 2560, 5194 |
| CfoI | GCGC | 2 cut(s) | 2869, 4084 |
| Cfr10I | RCCGGY | 1 cut(s) | 3677 |
| Cfr13I | GGNCC | 4 cut(s) | 1119, 1120, 1200, 4962 |
| Cfr42I | CCGCGG | 1 cut(s) | 264 |
| ClaI | ATCGAT | 1 cut(s) | 4656 |
| Csp6I | GTAC | 5 cut(s) | 21, 2759, 4784, 5499, 5544 |
| CviQI | GTAC | 5 cut(s) | 21, 2759, 4784, 5499, 5544 |
| DraI | TTTAAA | 1 cut(s) | 2385 |
| EaeI | YGGCCR | 2 cut(s) | 3675, 4857 |
| Eam1104I | CTCTTC | 9 cut(s) | 487, 1272, 1512, 1554, 1953, 2121, 2724, 4587, 4709 |
| EarI | CTCTTC | 9 cut(s) | 487, 1272, 1512, 1554, 1953, 2121, 2724, 4587, 4709 |
| Eco130I | CCWWGG | 4 cut(s) | 636, 1618, 4335, 4965 |
| Eco147I | AGGCCT | 1 cut(s) | 1162 |
| Eco24I | GRGCYC | 1 cut(s) | 1123 |
| Eco31I | GGTCTC | 1 cut(s) | 4373 |
| Eco32I | GATATC | 1 cut(s) | 1423 |
| Eco47I | GGWCC | 2 cut(s) | 1200, 4962 |
| Eco57I | CTGAAG | 6 cut(s) | 216, 546, 1578, 2439, 4565, 5532 |
| Eco81I | CCTNAGG | 2 cut(s) | 1035, 4974 |
| Eco88I | CYCGRG | 2 cut(s) | 1829, 2267 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1127 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1119, 1120 |
| EcoRV | GATATC | 1 cut(s) | 1423 |
| EcoT14I | CCWWGG | 4 cut(s) | 636, 1618, 4335, 4965 |
| EcoT22I | ATGCAT | 2 cut(s) | 1324, 5362 |
| EcoT38I | GRGCYC | 1 cut(s) | 1123 |
| ErhI | CCWWGG | 4 cut(s) | 636, 1618, 4335, 4965 |
| FaqI | GGGAC | 3 cut(s) | 1345, 2733, 4363 |
| FbaI | TGATCA | 3 cut(s) | 577, 3358, 5191 |
| FokI | GGATG | 6 cut(s) | 619, 713, 1820, 2488, 3005, 6077 |
| FriOI | GRGCYC | 1 cut(s) | 1123 |
| FspI | TGCGCA | 1 cut(s) | 4083 |
| GlaI | GCGC | 2 cut(s) | 2868, 4083 |
| GsuI | CTGGAG | 7 cut(s) | 1149, 1571, 2814, 3848, 4746, 5021, 5117 |
| HaeIII | GGCC | 5 cut(s) | 1121, 1162, 1623, 3677, 4859 |
| HapII | CCGG | 5 cut(s) | 1285, 1367, 2921, 3437, 3678 |
| HhaI | GCGC | 2 cut(s) | 2869, 4084 |
| Hin1I | GRCGYC | 2 cut(s) | 113, 5111 |
| Hin6I | GCGC | 2 cut(s) | 2867, 4082 |
| HinP1I | GCGC | 2 cut(s) | 2867, 4082 |
| HincII | GTYRAC | 4 cut(s) | 406, 984, 3868, 5053 |
| HindII | GTYRAC | 4 cut(s) | 406, 984, 3868, 5053 |
| HindIII | AAGCTT | 9 cut(s) | 50, 1865, 1970, 2117, 3147, 3458, 3776, 4316, 5435 |
| HpaI | GTTAAC | 3 cut(s) | 406, 3868, 5053 |
| HpaII | CCGG | 5 cut(s) | 1285, 1367, 2921, 3437, 3678 |
| HpyCH4IV | ACGT | 6 cut(s) | 113, 423, 2722, 4216, 5111, 5605 |
| HpySE526I | ACGT | 6 cut(s) | 113, 423, 2722, 4216, 5111, 5605 |
| Hsp92I | GRCGYC | 2 cut(s) | 113, 5111 |
| HspAI | GCGC | 2 cut(s) | 2867, 4082 |
| Kpn2I | TCCGGA | 1 cut(s) | 1366 |
| Ksp22I | TGATCA | 3 cut(s) | 577, 3358, 5191 |
| KspAI | GTTAAC | 3 cut(s) | 406, 3868, 5053 |
| KspI | CCGCGG | 1 cut(s) | 264 |
| LguI | GCTCTTC | 1 cut(s) | 1554 |
| MaeII | ACGT | 6 cut(s) | 113, 423, 2722, 4216, 5111, 5605 |
| MbiI | CCGCTC | 1 cut(s) | 2338 |
| MfeI | CAATTG | 5 cut(s) | 1302, 1671, 2931, 3296, 5786 |
| MflI | RGATCY | 5 cut(s) | 928, 1046, 3351, 3568, 5855 |
| MhlI | GDGCHC | 2 cut(s) | 446, 1123 |
| MmeI | TCCRAC | 3 cut(s) | 2352, 3238, 5571 |
| Mph1103I | ATGCAT | 2 cut(s) | 1324, 5362 |
| MroI | TCCGGA | 1 cut(s) | 1366 |
| MroXI | GAANNNNTTC | 8 cut(s) | 1095, 1398, 2089, 3372, 3777, 3978, 4107, 4233 |
| MslI | CAYNNNNRTG | 4 cut(s) | 219, 422, 2874, 3276 |
| MspA1I | CMGCKG | 6 cut(s) | 38, 263, 1727, 3826, 5219, 5492 |
| MspCI | CTTAAG | 2 cut(s) | 1930, 3214 |
| MspI | CCGG | 5 cut(s) | 1285, 1367, 2921, 3437, 3678 |
| MunI | CAATTG | 5 cut(s) | 1302, 1671, 2931, 3296, 5786 |
| MvnI | CGCG | 1 cut(s) | 263 |
| NciI | CCSGG | 1 cut(s) | 1286 |
| NcoI | CCATGG | 1 cut(s) | 4965 |
| NheI | GCTAGC | 1 cut(s) | 508 |
| NlaIV | GGNNCC | 5 cut(s) | 312, 1120, 1121, 2608, 4971 |
| NmeAIII | GCCGAG | 1 cut(s) | 284 |
| NmuCI | GTSAC | 4 cut(s) | 252, 1698, 3647, 5801 |
| NsbI | TGCGCA | 1 cut(s) | 4083 |
| NsiI | ATGCAT | 2 cut(s) | 1324, 5362 |
| NspI | RCATGY | 3 cut(s) | 491, 1361, 3998 |
| PaeR7I | CTCGAG | 2 cut(s) | 1829, 2267 |
| PagI | TCATGA | 3 cut(s) | 1852, 2560, 5194 |
| PceI | AGGCCT | 1 cut(s) | 1162 |
| PciI | ACATGT | 2 cut(s) | 487, 1357 |
| PciSI | GCTCTTC | 1 cut(s) | 1554 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 4857 |
| PdmI | GAANNNNTTC | 8 cut(s) | 1095, 1398, 2089, 3372, 3777, 3978, 4107, 4233 |
| PflFI | GACNNNGTC | 1 cut(s) | 4574 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 5480 |
| PfoI | TCCNGGA | 2 cut(s) | 2988, 4769 |
| PscI | ACATGT | 2 cut(s) | 487, 1357 |
| PshBI | ATTAAT | 2 cut(s) | 627, 3291 |
| PspN4I | GGNNCC | 5 cut(s) | 312, 1120, 1121, 2608, 4971 |
| PspOMI | GGGCCC | 1 cut(s) | 1119 |
| PspPI | GGNCC | 4 cut(s) | 1119, 1120, 1200, 4962 |
| PspXI | VCTCGAGB | 1 cut(s) | 2267 |
| PstI | CTGCAG | 1 cut(s) | 5250 |
| PstNI | CAGNNNCTG | 6 cut(s) | 281, 1019, 4423, 4484, 5347, 5744 |
| PsuI | RGATCY | 5 cut(s) | 928, 1046, 3351, 3568, 5855 |
| PsyI | GACNNNGTC | 1 cut(s) | 4574 |
| PvuII | CAGCTG | 5 cut(s) | 38, 1727, 3826, 5219, 5492 |
| RsaI | GTAC | 5 cut(s) | 22, 2760, 4785, 5500, 5545 |
| RsaNI | GTAC | 5 cut(s) | 21, 2759, 4784, 5499, 5544 |
| RseI | CAYNNNNRTG | 4 cut(s) | 219, 422, 2874, 3276 |
| SacII | CCGCGG | 1 cut(s) | 264 |
| SapI | GCTCTTC | 1 cut(s) | 1554 |
| Sau96I | GGNCC | 4 cut(s) | 1119, 1120, 1200, 4962 |
| SduI | GDGCHC | 2 cut(s) | 446, 1123 |
| SfcI | CTRYAG | 8 cut(s) | 174, 273, 1899, 1910, 2019, 3741, 5246, 5945 |
| Sfr274I | CTCGAG | 2 cut(s) | 1829, 2267 |
| Sfr303I | CCGCGG | 1 cut(s) | 264 |
| SgrBI | CCGCGG | 1 cut(s) | 264 |
| SinI | GGWCC | 2 cut(s) | 1200, 4962 |
| SlaI | CTCGAG | 2 cut(s) | 1829, 2267 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 219, 422, 2874, 3276 |
| SseBI | AGGCCT | 1 cut(s) | 1162 |
| SspI | AATATT | 3 cut(s) | 625, 5821, 6037 |
| StuI | AGGCCT | 1 cut(s) | 1162 |
| StyI | CCWWGG | 4 cut(s) | 636, 1618, 4335, 4965 |
| TaiI | ACGT | 6 cut(s) | 116, 426, 2725, 4219, 5114, 5608 |
| TaqI | TCGA | 8 cut(s) | 1830, 2268, 2856, 3237, 3307, 4634, 4656, 6080 |
| TatI | WGTACW | 3 cut(s) | 2758, 4783, 5543 |
| TauI | GCSGC | 2 cut(s) | 1760, 3389 |
| TseFI | GTSAC | 4 cut(s) | 252, 1698, 3647, 5801 |
| Tsp45I | GTSAC | 4 cut(s) | 252, 1698, 3647, 5801 |
| Tth111I | GACNNNGTC | 1 cut(s) | 4574 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 5480 |
| Vha464I | CTTAAG | 2 cut(s) | 1930, 3214 |
| VpaK11BI | GGWCC | 2 cut(s) | 1200, 4962 |
| VspI | ATTAAT | 2 cut(s) | 627, 3291 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1127 |
| XbaI | TCTAGA | 1 cut(s) | 3982 |
| XceI | RCATGY | 3 cut(s) | 491, 1361, 3998 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 74, 1194 |
| XhoI | CTCGAG | 2 cut(s) | 1829, 2267 |
| XmnI | GAANNNNTTC | 8 cut(s) | 1095, 1398, 2089, 3372, 3777, 3978, 4107, 4233 |
| ZraI | GACGTC | 2 cut(s) | 114, 5112 |
| Zsp2I | ATGCAT | 2 cut(s) | 1324, 5362 |