Rmu_sc0008509.1_g000028

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008509.1
OG0001092
Physical Location & Seq
Reverse (-)
119332 .. 121277
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008509.1_g000028.1.cds

Sequence Viewer

Length: 1782 bp
atgatcgatctgatttttaatatttggtccctgtacatttatcatcagcttcaacaattccttcagaagaaggataaaggcagtggaagccagggaaaatccacgaaaaagtctggaagatcagagcagcatggggctgtagctgatacagtctcaactgcaactaaacctgcagcgtccatcaagtctggaaaatcagagcagcatgaggctgttgctgatgcggtctcaactgcaactaaacccacagcatcgtctcacgtccgtgacggagaaattaagtctgctgtaggttctaattcaggaactgtcgacacatcagagtcaaattctgtagagaattctgcatactctgacactagtgtagctgttgctggtccctcggcagtgctcattacacaggagaaaagtgtggttgaaaccgaattgggggaaaatgccgaatcaccatcagaggaggtgggggttagtaagcgtgatgctgactcttctgttcagattgaaggtgagagtactgggactgctgatgctgaggtggcaagagacagttcattggacacttcacatacagtggattctgggggagaagccaagaattcaaagatgtctatacaggttgatgtatcagcgcaacatccttcagttgatattacagatgggatgtccgttactgttgaatcagagattccaagtagggaaaaggagtcattgccttcagtggaaaatattaatacatccttattgcaggagagggaagatcatgtaacagatgaagaattacagcagttaaatcccttgtctctccagcgtgaaagtgaaatcattgttccaagggaaagtttagctgttgttggtccctcggcagtgctcattacacaggagaaaagtgtggttgaaaccgaattgggggaaaatgccgaatcaccatcagaggaggtgggggttagtaagcgtgatgctgactcttctgttcagattgaaggtgagagtactgggactgctgatgctgaggtggcaagagacagttcattggacacttcacatacagtggattctgggggagaagccaagaattcaaagatgtctatacaggttgatgtatcagcgcaacatccttcagttgatattacagatgggatgtccgttactgttgaatcagagattccaagtagggaaaaggagtcattgccttcagtggaaaatattaatacatccttattgcaggagagggaagatcatgtaacagatgaagaattacagcagttaaatcccttgtctctccagcgtgaaagtgaaatcattgttccaagggaaagtttagctgttgttggtccctcggcagtgctcattacacaggagaaaagtgtggttgaaaccgaattgggggaaaatgccgaatcaccatcagaggaggtgggggttagtaagcgtgatgctgactcttctgttcagattgaaggtgagagtactgggactgctgatgctgaggtggcaagagacagttcattggacacttcacatacagtggattctgggggagaagccaagaattcaaagatgtctatacaggttgatgtatcagcgcaacatccttcagttgatattacagatgggatgtccgttactgttgaatcagagattccaagtagggaaaaggagtcattgccttcagtggaaaatattaatacatccttattgcaggagagggaagatcatgtaacagatgtacgttgtgctcttctccttttgttatttaattttcattgcactttttggatctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

593

Amino Acids

63.14

Weight (kDa)

4.37

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 312
AciI CCGC 1 cut(s) 224
AcsI RAATTY 5 cut(s) 328, 340, 595, 1072, 1549
AcuI CTGAAG 7 cut(s) 47, 624, 699, 1101, 1176, 1578, 1653
AfaI GTAC 5 cut(s) 35, 514, 991, 1468, 1728
AfiI CCNNNNNNNGG 3 cut(s) 429, 906, 1383
AhlI ACTAGT 1 cut(s) 359
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 90
AleI CACNNNNGTG 1 cut(s) 264
AluBI AGCT 5 cut(s) 49, 143, 368, 845, 1322
AluI AGCT 5 cut(s) 49, 143, 368, 845, 1322
Alw21I GWGCWC 4 cut(s) 393, 870, 1347, 1738
Alw26I GTCTC 8 cut(s) 157, 232, 261, 537, 804, 1014, 1281, 1491
AlwNI CAGNNNCTG 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 127, 173, 202
ApoI RAATTY 5 cut(s) 328, 340, 595, 1072, 1549
AseI ATTAAT 3 cut(s) 729, 1206, 1683
AspLEI GCGC 3 cut(s) 631, 1108, 1585
AspS9I GGNCC 4 cut(s) 27, 377, 854, 1331
AsuHPI GGTGA 6 cut(s) 438, 518, 915, 995, 1392, 1472
AvaII GGWCC 4 cut(s) 27, 377, 854, 1331
Bbv12I GWGCWC 4 cut(s) 393, 870, 1347, 1738
BbvCI CCTCAGC 3 cut(s) 531, 1008, 1485
BbvI GCAGC 3 cut(s) 139, 185, 214
BccI CCATC 7 cut(s) 188, 457, 650, 934, 1127, 1411, 1604
BciT130I CCWGG 1 cut(s) 92
BcoDI GTCTC 8 cut(s) 157, 232, 261, 537, 804, 1014, 1281, 1491
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 4 cut(s) 138, 171, 288, 333
BfuAI ACCTGC 1 cut(s) 178
BisI GCNGC 3 cut(s) 128, 174, 203
BlsI GCNGC 3 cut(s) 129, 175, 204
BmcAI AGTACT 3 cut(s) 514, 991, 1468
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 4 cut(s) 27, 377, 854, 1331
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 27, 377, 854, 1331
BmiI GGNNCC 4 cut(s) 29, 379, 856, 1333
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 3 cut(s) 525, 1002, 1479
BmsI GCATC 8 cut(s) 211, 260, 469, 517, 946, 994, 1423, 1471
BmuI ACTGGG 3 cut(s) 525, 1002, 1479
BpmI CTGGAG 2 cut(s) 788, 1265
Bpu10I CCTNAGC 3 cut(s) 531, 1008, 1485
Bsa29I ATCGAT 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 6 cut(s) 91, 381, 830, 858, 1307, 1335
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 906, 1383
Bse1I ACTGG 3 cut(s) 520, 997, 1474
Bse3DI GCAATG 4 cut(s) 707, 1184, 1661, 1762
BseBI CCWGG 1 cut(s) 92
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 6 cut(s) 91, 381, 830, 858, 1307, 1335
BseGI GGATG 9 cut(s) 634, 666, 734, 1111, 1143, 1211, 1588, 1620, 1688
BseLI CCNNNNNNNGG 3 cut(s) 429, 906, 1383
BseMI GCAATG 4 cut(s) 707, 1184, 1661, 1762
BseMII CTCAG 3 cut(s) 522, 999, 1476
BseNI ACTGG 3 cut(s) 520, 997, 1474
BseRI GAGGAG 3 cut(s) 470, 947, 1424
BseXI GCAGC 3 cut(s) 139, 185, 214
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 4 cut(s) 393, 870, 1347, 1738
BslFI GGGAC 7 cut(s) 13, 363, 532, 840, 1009, 1317, 1486
BslI CCNNNNNNNGG 3 cut(s) 429, 906, 1383
BsmAI GTCTC 8 cut(s) 157, 232, 261, 537, 804, 1014, 1281, 1491
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 7 cut(s) 13, 363, 532, 840, 1009, 1317, 1486
Bso31I GGTCTC 1 cut(s) 232
Bsp1286I GDGCHC 4 cut(s) 393, 870, 1347, 1738
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
BspACI CCGC 1 cut(s) 224
BspCNI CTCAG 3 cut(s) 523, 1000, 1477
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 4 cut(s) 29, 379, 856, 1333
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 178
BspQI GCTCTTC 1 cut(s) 1743
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 4 cut(s) 707, 1184, 1661, 1762
BsrGI TGTACA 1 cut(s) 33
BsrI ACTGG 3 cut(s) 520, 997, 1474
BssECI CCNNGG 6 cut(s) 91, 381, 830, 858, 1307, 1335
BssMI GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
BssT1I CCWWGG 2 cut(s) 830, 1307
Bst2UI CCWGG 1 cut(s) 92
Bst6I CTCTTC 4 cut(s) 493, 970, 1447, 1743
BstAUI TGTACA 1 cut(s) 33
BstDEI CTNAG 3 cut(s) 531, 1008, 1485
BstF5I GGATG 9 cut(s) 634, 666, 734, 1111, 1143, 1211, 1588, 1620, 1688
BstHHI GCGC 3 cut(s) 631, 1108, 1585
BstKTI GATC 7 cut(s) 6, 10, 122, 760, 1237, 1714, 1779
BstMAI GTCTC 8 cut(s) 157, 232, 261, 537, 804, 1014, 1281, 1491
BstMBI GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
BstMWI GCNNNNNNNGC 4 cut(s) 87, 536, 1013, 1490
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 4 cut(s) 138, 171, 288, 333
BstV1I GCAGC 3 cut(s) 139, 185, 214
BstX2I RGATCY 1 cut(s) 1776
BstYI RGATCY 1 cut(s) 1776
Bsu15I ATCGAT 1 cut(s) 6
BsuTUI ATCGAT 1 cut(s) 6
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 9 cut(s) 634, 666, 734, 1111, 1143, 1211, 1588, 1620, 1688
BtsI GCAGTG 4 cut(s) 88, 393, 870, 1347
BveI ACCTGC 1 cut(s) 178
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 3 cut(s) 631, 1108, 1585
Cfr13I GGNCC 4 cut(s) 27, 377, 854, 1331
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 1 cut(s) 165
Csp6I GTAC 5 cut(s) 34, 513, 990, 1467, 1727
CviAII CATG 5 cut(s) 131, 206, 761, 1238, 1715
CviQI GTAC 5 cut(s) 34, 513, 990, 1467, 1727
DdeI CTNAG 3 cut(s) 531, 1008, 1485
DpnI GATC 7 cut(s) 5, 9, 121, 759, 1236, 1713, 1778
DpnII GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
Eam1104I CTCTTC 4 cut(s) 493, 970, 1447, 1743
EarI CTCTTC 4 cut(s) 493, 970, 1447, 1743
Eco130I CCWWGG 2 cut(s) 830, 1307
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 4 cut(s) 27, 377, 854, 1331
Eco57I CTGAAG 7 cut(s) 47, 624, 699, 1101, 1176, 1578, 1653
EcoRI GAATTC 4 cut(s) 340, 595, 1072, 1549
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 2 cut(s) 830, 1307
ErhI CCWWGG 2 cut(s) 830, 1307
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 5 cut(s) 134, 209, 764, 1241, 1718
FaqI GGGAC 7 cut(s) 13, 363, 532, 840, 1009, 1317, 1486
FatI CATG 5 cut(s) 130, 205, 760, 1237, 1714
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 128, 174, 203
FokI GGATG 9 cut(s) 621, 673, 721, 1098, 1150, 1198, 1575, 1627, 1675
Fsp4HI GCNGC 3 cut(s) 128, 174, 203
FspBI CTAG 1 cut(s) 360
GlaI GCGC 3 cut(s) 630, 1107, 1584
GluI GCNGC 3 cut(s) 128, 174, 203
GsuI CTGGAG 2 cut(s) 788, 1265
HgaI GACGC 1 cut(s) 165
HhaI GCGC 3 cut(s) 631, 1108, 1585
Hin1II CATG 5 cut(s) 134, 209, 764, 1241, 1718
Hin6I GCGC 3 cut(s) 629, 1106, 1583
HinP1I GCGC 3 cut(s) 629, 1106, 1583
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HphI GGTGA 6 cut(s) 438, 518, 915, 995, 1392, 1472
Hpy166II GTNNAC 1 cut(s) 313
Hpy188III TCNNGA 3 cut(s) 114, 189, 303
Hpy8I GTNNAC 1 cut(s) 313
HpyCH4IV ACGT 2 cut(s) 261, 1729
HpyCH4V TGCA 8 cut(s) 161, 173, 236, 347, 745, 1222, 1699, 1767
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 536, 1013, 1490
HpyF3I CTNAG 3 cut(s) 531, 1008, 1485
HpySE526I ACGT 2 cut(s) 261, 1729
Hsp92II CATG 5 cut(s) 134, 209, 764, 1241, 1718
HspAI GCGC 3 cut(s) 629, 1106, 1583
Kzo9I GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
LguI GCTCTTC 1 cut(s) 1743
Lsp1109I GCAGC 3 cut(s) 139, 185, 214
LweI GCATC 8 cut(s) 211, 260, 469, 517, 946, 994, 1423, 1471
MaeI CTAG 1 cut(s) 360
MaeII ACGT 2 cut(s) 261, 1729
MaeIII GTNAC 7 cut(s) 266, 667, 763, 1144, 1240, 1621, 1717
MalI GATC 7 cut(s) 5, 9, 121, 759, 1236, 1713, 1778
MboI GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
MflI RGATCY 1 cut(s) 1776
MhlI GDGCHC 4 cut(s) 393, 870, 1347, 1738
MlyI GAGTC 7 cut(s) 332, 479, 713, 956, 1190, 1433, 1667
MseI TTAA 8 cut(s) 18, 279, 729, 788, 1206, 1265, 1683, 1755
MslI CAYNNNNRTG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 87, 536, 1013, 1490
NdeII GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
NlaIII CATG 5 cut(s) 134, 209, 764, 1241, 1718
NlaIV GGNNCC 4 cut(s) 29, 379, 856, 1333
NmeAIII GCCGAG 3 cut(s) 362, 839, 1316
NmuCI GTSAC 1 cut(s) 266
OliI CACNNNNGTG 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 1743
PkrI GCNGC 3 cut(s) 129, 175, 204
PleI GAGTC 7 cut(s) 331, 479, 712, 956, 1189, 1433, 1666
PpsI GAGTC 7 cut(s) 331, 479, 712, 956, 1189, 1433, 1666
PshBI ATTAAT 3 cut(s) 729, 1206, 1683
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 4 cut(s) 29, 379, 856, 1333
PspPI GGNCC 4 cut(s) 27, 377, 854, 1331
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 1 cut(s) 1776
RsaI GTAC 5 cut(s) 35, 514, 991, 1468, 1728
RsaNI GTAC 5 cut(s) 34, 513, 990, 1467, 1727
RseI CAYNNNNRTG 1 cut(s) 264
SalI GTCGAC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 1743
SaqAI TTAA 8 cut(s) 18, 279, 729, 788, 1206, 1265, 1683, 1755
SatI GCNGC 3 cut(s) 128, 174, 203
Sau3AI GATC 7 cut(s) 3, 7, 119, 757, 1234, 1711, 1776
Sau96I GGNCC 4 cut(s) 27, 377, 854, 1331
ScaI AGTACT 3 cut(s) 514, 991, 1468
SchI GAGTC 7 cut(s) 332, 479, 713, 956, 1190, 1433, 1667
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 4 cut(s) 393, 870, 1347, 1738
SfaNI GCATC 8 cut(s) 211, 260, 469, 517, 946, 994, 1423, 1471
SfcI CTRYAG 4 cut(s) 138, 171, 288, 333
SinI GGWCC 4 cut(s) 27, 377, 854, 1331
SmiMI CAYNNNNRTG 1 cut(s) 264
SpeI ACTAGT 1 cut(s) 359
SsiI CCGC 1 cut(s) 224
SspI AATATT 4 cut(s) 22, 727, 1204, 1681
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 2 cut(s) 830, 1307
TaiI ACGT 2 cut(s) 264, 1732
TaqI TCGA 2 cut(s) 6, 312
TatI WGTACW 4 cut(s) 33, 512, 989, 1466
Tru1I TTAA 8 cut(s) 18, 279, 729, 788, 1206, 1265, 1683, 1755
Tru9I TTAA 8 cut(s) 18, 279, 729, 788, 1206, 1265, 1683, 1755
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 3 cut(s) 127, 173, 202
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 6 cut(s) 540, 786, 1017, 1263, 1494, 1751
TspGWI ACGGA 5 cut(s) 254, 285, 655, 1132, 1609
VpaK11BI GGWCC 4 cut(s) 27, 377, 854, 1331
VspI ATTAAT 3 cut(s) 729, 1206, 1683
XapI RAATTY 5 cut(s) 328, 340, 595, 1072, 1549
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 1 cut(s) 360
ZrmI AGTACT 3 cut(s) 514, 991, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.