Rorug06G0337600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001092
Physical Location & Seq
Forward (+)
48971364 .. 48971887
524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0337600.1

Sequence Viewer

Length: 417 bp
ATGAGTTCTCCGACTTCCAAGGGCGGCCGAGGCAAGCCCAAGGCCACCAAATCCGTCTCCAGGTCCTCCAAGGCCGGCCTCCAATTCCCCGTCGGCCGTATCGCTCGCTTCCTCAAATCCGGCAAGTACGCCGAGCGAGTCGGCGCCGGCGCTCCCGTCTACCTCTCCGCCGTCCTCGAGTATCTCGCCGCTGAGGTTCTTGAGCTCGCTGGAAATGCCGCTAGAGATAACAAGAAGAATCGGATCGTTCCGAGGCACATTCAGCTGGCTGTGCGTAATGACGAGGAGTTGAGCAAGCTTTTGGGGACTGTGACTATTGCTAATGGTGGTGTCTTGCCTAACATTCATCAGAATCTGCTGCCGAAGAAGACTGGGAAAGGCAAGGGAGACATTGGATCGGCCTCGCAAGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

14.58

Weight (kDa)

10.36

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 13 - 92 7.5e-15 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 95 - 128 1.6e-19 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 159
AciI CCGC 4 cut(s) 24, 168, 189, 219
AclWI GGATC 2 cut(s) 251, 403
AcoI YGGCCR 2 cut(s) 25, 94
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 60
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 3 cut(s) 205, 265, 298
AluI AGCT 3 cut(s) 205, 265, 298
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 61, 381
AlwI GGATC 2 cut(s) 251, 403
AlwNI CAGNNNCTG 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 6 cut(s) 25, 42, 72, 76, 94, 399
ApeKI GCWGC 1 cut(s) 358
AspLEI GCGC 2 cut(s) 146, 152
AspS9I GGNCC 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 63
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 207
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 345
BceAI ACGGC 2 cut(s) 81, 155
BciT130I CCWGG 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 61, 381
BfaI CTAG 1 cut(s) 222
BfoI RGCGCY 2 cut(s) 147, 153
BisI GCNGC 4 cut(s) 25, 189, 219, 359
BlsI GCNGC 4 cut(s) 26, 190, 220, 360
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 63
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 145
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 381
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 374
BpmI CTGGAG 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 5 cut(s) 18, 28, 39, 69, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse118I RCCGGY 2 cut(s) 74, 146
Bse1I ACTGG 1 cut(s) 376
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 5 cut(s) 18, 28, 39, 69, 251
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 1 cut(s) 299
BseX3I CGGCCG 2 cut(s) 25, 94
BseXI GCAGC 1 cut(s) 345
Bsh1285I CGRYCG 2 cut(s) 28, 97
BshFI GGCC 6 cut(s) 27, 44, 74, 78, 96, 401
BshNI GGYRCC 1 cut(s) 143
BsiEI CGRYCG 2 cut(s) 28, 97
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 3 cut(s) 75, 120, 147
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 2 cut(s) 61, 381
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 6 cut(s) 27, 44, 74, 78, 96, 401
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 243, 395
BspACI CCGC 4 cut(s) 24, 168, 189, 219
BspANI GGCC 6 cut(s) 27, 44, 74, 78, 96, 401
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 145
BspPI GGATC 2 cut(s) 251, 403
BspT107I GGYRCC 1 cut(s) 143
BsrFI RCCGGY 2 cut(s) 74, 146
BsrI ACTGG 1 cut(s) 376
BssAI RCCGGY 2 cut(s) 74, 146
BssECI CCNNGG 5 cut(s) 18, 28, 39, 69, 251
BssMI GATC 2 cut(s) 243, 395
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 3 cut(s) 18, 39, 69
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 310
BstACI GRCGYC 1 cut(s) 144
BstC8I GCNNGC 7 cut(s) 35, 76, 106, 148, 207, 267, 296
BstDEI CTNAG 1 cut(s) 192
BstH2I RGCGCY 2 cut(s) 147, 153
BstHHI GCGC 2 cut(s) 146, 152
BstKTI GATC 2 cut(s) 246, 398
BstMAI GTCTC 2 cut(s) 61, 381
BstMBI GATC 2 cut(s) 243, 395
BstMCI CGRYCG 2 cut(s) 28, 97
BstMWI GCNNNNNNNGC 4 cut(s) 30, 215, 262, 271
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 345
BstV2I GAAGAC 1 cut(s) 374
BstZI CGGCCG 2 cut(s) 25, 94
BsuRI GGCC 6 cut(s) 27, 44, 74, 78, 96, 401
Cac8I GCNNGC 7 cut(s) 35, 76, 106, 148, 207, 267, 296
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 2 cut(s) 146, 152
Cfr10I RCCGGY 2 cut(s) 74, 146
Cfr13I GGNCC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 127
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 1 cut(s) 192
DinI GGCGCC 1 cut(s) 145
DpnI GATC 2 cut(s) 245, 397
DpnII GATC 2 cut(s) 243, 395
EaeI YGGCCR 2 cut(s) 25, 94
EagI CGGCCG 2 cut(s) 25, 94
EciI GGCGGA 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 205
EclXI CGGCCG 2 cut(s) 25, 94
Eco130I CCWWGG 3 cut(s) 18, 39, 69
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 63
Eco52I CGGCCG 2 cut(s) 25, 94
Eco53kI GAGCTC 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 3 cut(s) 18, 39, 69
EcoT38I GRGCYC 1 cut(s) 207
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 3 cut(s) 18, 39, 69
Esp3I CGTCTC 1 cut(s) 61
FaqI GGGAC 1 cut(s) 319
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 25, 189, 219, 359
FriOI GRGCYC 1 cut(s) 207
FseI GGCCGGCC 1 cut(s) 78
Fsp4HI GCNGC 4 cut(s) 25, 189, 219, 359
FspBI CTAG 1 cut(s) 222
GlaI GCGC 2 cut(s) 145, 151
GluI GCNGC 4 cut(s) 25, 189, 219, 359
GsuI CTGGAG 1 cut(s) 43
HaeII RGCGCY 2 cut(s) 147, 153
HaeIII GGCC 6 cut(s) 27, 44, 74, 78, 96, 401
HapII CCGG 3 cut(s) 75, 120, 147
HhaI GCGC 2 cut(s) 146, 152
Hin1I GRCGYC 1 cut(s) 144
Hin6I GCGC 2 cut(s) 144, 150
HinP1I GCGC 2 cut(s) 144, 150
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 3 cut(s) 138, 238, 352
HpaII CCGG 3 cut(s) 75, 120, 147
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 4 cut(s) 12, 243, 252, 351
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 215, 262, 271
HpyF3I CTNAG 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 144
HspAI GCGC 2 cut(s) 144, 150
KasI GGCGCC 1 cut(s) 143
KroI GCCGGC 2 cut(s) 74, 146
KroNI GCCGGC 2 cut(s) 76, 148
Kzo9I GATC 2 cut(s) 243, 395
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 8 cut(s) 46, 73, 88, 133, 160, 195, 251, 357
Lsp1109I GCAGC 1 cut(s) 345
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 310
MalI GATC 2 cut(s) 245, 397
MboI GATC 2 cut(s) 243, 395
MboII GAAGA 3 cut(s) 247, 376, 379
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 1 cut(s) 83
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 147
MmeI TCCRAC 1 cut(s) 35
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 2 cut(s) 74, 146
MspA1I CMGCKG 2 cut(s) 191, 265
MspI CCGG 3 cut(s) 75, 120, 147
MspR9I CCNGG 1 cut(s) 61
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 4 cut(s) 30, 215, 262, 271
NaeI GCCGGC 2 cut(s) 76, 148
NarI GGCGCC 1 cut(s) 144
NdeII GATC 2 cut(s) 243, 395
NgoMIV GCCGGC 2 cut(s) 74, 146
NlaIV GGNNCC 1 cut(s) 145
NmeAIII GCCGAG 2 cut(s) 53, 157
NmuCI GTSAC 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 176
PdiI GCCGGC 2 cut(s) 76, 148
PfeI GAWTC 2 cut(s) 238, 352
PkrI GCNGC 4 cut(s) 26, 190, 220, 360
PleI GAGTC 1 cut(s) 146
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 1 cut(s) 63
PspPPI RGGWCCY 1 cut(s) 63
PspXI VCTCGAGB 1 cut(s) 176
PstNI CAGNNNCTG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 265
RigI GGCCGGCC 1 cut(s) 78
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacI GAGCTC 1 cut(s) 207
SatI GCNGC 4 cut(s) 25, 189, 219, 359
Sau3AI GATC 2 cut(s) 243, 395
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 207
SetI ASST 6 cut(s) 65, 165, 198, 207, 267, 300
SfoI GGCGCC 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 176
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 63
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 176, 200
SmoI CTYRAG 2 cut(s) 176, 200
Sse9I AATT 1 cut(s) 83
SsiI CCGC 4 cut(s) 24, 168, 189, 219
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 1 cut(s) 222
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 3 cut(s) 18, 39, 69
TaaI ACNGT 1 cut(s) 310
TaqI TCGA 1 cut(s) 177
TasI AATT 1 cut(s) 83
TauI GCSGC 3 cut(s) 27, 191, 221
TfiI GAWTC 2 cut(s) 238, 352
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 335
TspGWI ACGGA 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 63
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.