RchiOBHm_Chr6g0305121

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001092
Physical Location & Seq
Forward (+)
64193551 .. 64202819
9269 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27420

Sequence Viewer

Length: 6861 bp
ATGTCCGTCACTGTTGAATCAGATATTCCGAGTAGGGAAAAGGAGTCAATGCCTTCACGGGAAAATATTAATACATCCTTAATGCAGGAGAGGGAAGATCAGGAAGAATTACAGCAGTTAAATGCCTTGTCTCTCCAGTGTGAAAGTGAAATCATTGTTCCAAGGGAAAGTTTAGCTGTTGTTGGTCCCTCGGCAGTGCCAATTACAGAGGAGAAAAGTGTGGTTGAAACCGAATTGGAGAAAAATGCCGAATCGTCATCAGAGGAAGTGGGGGTTAGTAAGCGTGATGCTGACTCTTCTGTTCAGAATGAAGGTGAGAGTACTGGGACTGCTGGTGCTGAGATGGCAAAAGACAACTCATTGGGCACTTCACATACAGTGGATTCTGGAGGAGAAGCCGAAAATTCAAATGTGTCTATACAGGTTGATGAATCGGCCCAACATGCTTCAGTCGATATTACAGAGGGGATGTCCGTCACTGTTGAATTAGAGATTCCGAGTAGGGAAAAGGAGTCAATGCCTTCACGGGAAAATATTAATACATCCTTAATGCAGGAGAGGGAAGATCAGGTAACAGATGAAGAAGTACAGCAGTTAAATGCCTTGTCTCTCCAGCCTTGTCTCTCAACTGCAACTAAACCTACAGCATCGTCTCGGGTCTGTGACGGAGAAATTAAGTCTGTTGTATGTTCTAATTCAGGAATTGTCAACACATCAGAGTCAAATTCTGTAGAGAATTCTGTAGACTCTGACACTAGTGTAGCTGTTGCTGGTCCCTCAGCAGTGCCCATTACACAGGAGAAAAGTGTGGTTGAAACTGAATTGGAGAAAAATGCCGAATCACCATCAGAGGAAGTGGGGGTTAGTAAGCGTGATGCTGACTCCTCTGTTCAGAATGAAGGTGAGAGTACTGGGACTGCTGATGCTGAGGTGGCAAGAGGCATTTCATTGGACACTTCACATACAGTGGATTCTGGAGGAGAAGCCAAGAATTCAAAGATGTCTATACAGGTTGATGTATCAGCGCAACATGCTTCTGATAATATTACAGAGGGGATGTCCGTTATTGTTGAATCAGAGATTCCAAGTAGTGAAGAGGAGTCAATGCCTTCACAGGACAATATTAATACATCCTTAATGCAGGATAGGGAAGATCAGGAAGAAGTACAGCAGTTGAATTCCTTGTCTCTGGGGAATGCTGATGTCACCTCTGGGGAATTGAAACATCAGAATGAGGCCTTAGAAGAATATAGCAAGAAACTGGAAGCAACAAACATTGAGCTCAGGGTCCTATATGAAGCTTTAGAAGAACATAGGGGAAGCGTTGAATCTAGGAACTGTGAGCTTTCGATTCTCTGCGAATCCTTACAACTAGAAGTCACTAATCTTAAAGTAGAAAACGTGGAAGTTGACAGAAAACTACATGTGTATGAGGCCACCTCTGGGGAGTTGAAGGATCAGAATGAGGCCTTAGAAGAATATAGTAAGAAACTGGAAGCAACGAACATTGAGCGCAGGGTCCTATATGAAGCTTTAGAAGAACATAGGGGAAGGATTGAATCTAGGAACTGTGAGCTTTCGATTCTCTGCGAATCCTTACAACTAGAGGTCACTAATCTTAAAGTAGAAAACGTGGAAGTTGACAGAAAGCTACATGTGTATGAATCAAGAACTAGTAAATTGCAGAGTCAATTGCATGATCTACATCTAATTTCAAATGTTATGGTATCTCAGATCTCTGAGCAGTTGGAAAATTTTCACAAGGAAGCAGCTGAGAAAGTTTTGATACTTGAGCGCCATTGGAATTCAACTATTGATCCGGTTCTTGAAGCAACTGGGAAGCTTGATGAATCTCTTGGCAGGGTCAACATGACTACAGCAGTCTCTCATGATTCTTTGGACAGAATTAGCTATTCTGTTGCTTCTGTTTATGATGCCATCAGTTTTATTCAAGATCTGAAGGACAAACTTGAAAGTTCTCAAACAGAACATGAAGCAGTCTTTACTTTATACAAAGAAGCGACTGAGAAATGTGATGATCTGCATGGAAAGAATCAAATGGCCAGTGATTTGTTGCAGAAGTTGTATGGCGACCTTTCTGAACTTCTCACGGTTTTGCATGGGTCTACAGATGGAAGTGATATGTACTTAGAACCTGAGAAGCTTCCTGATCCTCTAGATTACAGTAATTATGAGATCATCATAGAACATGTGGAATCTTTTTTGAGGGGGAGTCTGCAACTTGAATCTGTTAACAAGAAACTAAATTCAGAGTTGATGGCTAGCGCTGAAGAAGTTGAGGAACTGAAACAAAGATGCCTTGATTCTAATGTTCTGCAGAAGTTAATAGAAAATGTTGAAGGGGTGCTGAAAGTGGAACATGCTGAGATTCAATTGGATAAAACGCCTGCTTCACGTCTTGAATCACTGGTGTCATGTCTTGTTCGGAAATGCGAGGAGGCTGACGTGCAGCTAAGCTTATCTAAGGAAGATTCTGGATCTAAGGAGGTGGAGCTGACTTTAATGCAGGAAGAAGTACAGCAGTTAAATGCCTTGTCTCTCCAGCATGAAAGTGAACTCATTGTTCTAAGGGAAAGTTTACATCAGGCGGAGGAAGCACTTCTTGTTGCTCGTTCTGAGATAGAAGGGAAAGTAAATGAACTTGAACAGTCAGAGCAACGGGTGTCTTCCCTTAGAGAGAAGCTTACCATTGCTGTCACCAAGGGGAAAGGTTTGATTGTGCAGCGAGATGGTCTGAAGCAGTCGCTTAACGAGAAATCTGTTGAACTGGAAAGATTCTCACAGGAACTGCAAATGAAAGATGCAAGGCTTCTTGAGATTGAAACAAAACTTAAGGCATATTCAGAGTCAGGTGAGCGGGTGGAAGCTCTGGAATCTGAGCTTTCATATATTCGCAATTCGGCTACTGCATTAAGAGAATCATTCCTTCTCAAAGATTCTGTCCTTCAGAGAATAGAAGAGATCTTGGAAGACCTTGATCTGCCAGAGCATTTTCATTCAAGAGATATTATTGAGAAGATTGATTGGCTGGCTAGGACAGCTACCAGTAACACTTTTCCTGTGACTGATTCAGATCAGAAGAGTTCTGCCGGTGGAGTTTCGTACTCTGATACTGGTTTTGTTGTTATGGATTCCTGGAAAGATGATTTACAACCAAGTTCAAATTCAAGTGAGGATATCAAAAGAAAATATGACGAACTTCAGAGTAAATTTTATGGGTTGGCTGAACAGAATGAAATGTTGGAACAATCTTTAATGGAAAGGAACAACATAGTACAGAGATGGGAGGAAATTTTGGACAGAATTGACATGCCATCACATCTGCGATCTGTGGAGCCAGAGGATAGGATCAATTGGTTAAGTAAAGCACTTTCAGAAGTGCAGGAAGACAATATGTCTCTCCAGCAGAAGGTTGTTAACCTTGAAAACCATTGTGTATCACTAACTGCTGATTTGGAAGACTCGCAAAGGAGAGTGTCTGACCTTGAGGCAGACCTTCAAACATTTGTCCATGAGAGAGATCATCTTTCTGAAAGACTGGAGACTCTGGTCAATGATCAGGAGAAGCTTTCAACAAAGGCAGTTGAATTTGAGCTTGAGAATGAAAAGCTGCAGAAGGAAGTCACTGATTTGCAAGAAAATGTAGCCAAGATGCATGGAAATGAGAATCAAATTCTCAGCATAGAAGCTGACTTAAGAAGATTACAGAGTTTGGTTACTGATGCCTTGGAGGTCTCTGGATCAAAGTATGAGTACTCCAGCGATAGTAGCATTGAGTGCTTGGAAGGGTTACTGAATAAGCTTTTAGAGAGTTATGCAACTCTTTCCTCAGGAAAACCTGTGCATGGGGGTGCAGCTGAAGGTCTCCATACTGAATATACTGATGCAACAGTTGTTGGATCGAGAAGCCTAAATAGACTTGATTCCCAGGAATCAGATATAGATGTTTTGAAGAAAGAGTTGGAGGATGTTCAACATGAACTTTTAGATGTGAAGGAGGAGAGGGATGGATATCTAGAGAAGCAACAGTCTATGGCTAGTGAATTTGAAGCATTACATAACAAAGTGAATGAGTTGCAAGGGCTGCTTAATCAAGAGGAGAAGAAGTCAGCTTCTGTTAGAGAGAAGTTAAATGTTGCAGTCAGAAAAGGGAAGTCTTTGGTACAACAGCGTGACAGTCTGAAACAAAGTATTGATGAGGTCAACTCTGAGGTGGAACGTTTGAGATCAGAGATCACGACGGGGCAAGTTAAAATTGCAGAGTATGAACAAAACTTCAGGGATTTGTCTACTTACCCTGGTAGGGTAGAAGCCTTAGAATCTGAGACATTGTTCTTGAGGAATTGTTTGAATGAAACTGAGCAGAATTTGCAGCAGAAAGCAAATACTTTGAGCATGATTGTAAATATCTTAGACAACATTGATGTAGGAGGCGATTTTAATTCTCGTGATCCAGTTGTGAAGTTGGAACAAATTGGGAAAATGTGCTTTGATTTACGCACAGATGTAGCCTCTTCTGAGCAAGAGACTAGGAAATCCAAAAGAGCAGCAGAGCTACTCCTTGCAGAACTGAATGAGGTTCAAGAGAGGAATGACGGTCTCCAGGAAGAGCTAGCAAAATCTGCTGATGAAATTTCTATACTCTCCAAGGAAAGGGATCTTGCTGAGGCTGGCAAGCTTGAAGCTGTTTTAAGTCTTGAAAAGTTATCCACTGCTCACTCTGAGGAAAGAAACCAATTTTCTGAATTTGCAGAACTGAAATCTGGTGTAGATCAACTCAGGAAGGGTTTCCATGATATTAGCAATTCATTGGCTGGTCTTTTTTACAACGACTTGGAATTTTTGAAAAACTTGGAGTCTGGTATTGACTCATGTCTCAACTTGAATAGTGGTAATGTAGTTGATGTGCACCCCTTCACTGCAGCTGGTGGATTTCTATTGAGCAAATCAGATAAGGATAACTCTATTTCAATTCATTCTTGGTCAGACGAGGCGCATGGACATTTTGGTGACAGTTTTGCCATCGAAACCTTTACATACATAACACATTATGTGCAAGAGCTCATGACGGAGATTGGTGTGCTTAAAGAAAAATTAGATGAACACTCAATGTCTTTGCATGAAAAATCTAGCAGTGTATCCAGATTGGTGGCAATTGTTCGTGGAGAGATAAGTTCCAAAAATGAGTCGTTTGAAGCCTTGAGGAGAGACTTTTTGCAGATGGAAATGGTCAAAAAGGAAAAGGACAAGGAACTTCTTGTCTTGCATAGAAATGCTGCCTTGCTTTTTGAAGCATGCACTAGTTCAGTTGTGGAAATAAATAGAAGAAAAGCTGAATTGGTGGGAAATTGTTGGGCTGTTGGAGATCTGGGAATGACATCAAAAACAGCAGAATTGCCCGCTTTCAGGGGAGAGGGTGAGCTGTACTCTGAGGAATCTGTTAGGTCTGTGGCAGACAGGCTTTTGTCGGCTGCTAGTGATTTTGCTACTCTGACAGCTGAAATAGCTGAAGGCAGCCAAAAGGAAATGAAGCTTACCATTTCAAATTTGCAGAAAGAGCTTCAGGAGAAGGACGTTCAAAAGGAGAGGATTTTCATGGAGCTTGTAAGTCAAATCAAGGAGGCTGAAGCTACTGCAAGCAGCTATTCAGTGGACCTTGAATCTTCACAAACCTTGGTGCATGATTTGGAGAAACAGTTGGAAGCGATGAAAGGTGAACGCAACCTACTTGAGCAGAGAGTAAAGGAGCTAGAAGATGACCATGCCTCCTCTGACGAGTTACAACAGAGGGTCAGATCACTAACGGATGTGCTTGCTGCCAAAGACCATGAAATTGAGGAGCTAATGCAAGCATTTGATGAGGAGGAGGTACAGATGCATGGTATCACCTTCAAGATCAAGGAACTGGAAAAGATTGTTGAACAAAAAAACCTAGATTTAGAGAACCTTGAAGCTTCTCGTGGCAAGGTGATGAAAAAGCTCTTTGTCACTGTGAATAAGTTTGATGAGCTGCATCATCTATCTGCAAGCCTCCTTGCGGAAGTTGAAGAACTGCAGTCACAGTTGCAAGATCGGGATGCAGAGATTTCTTTCTTAAGGCAAGAGGTCACTAGGTGCACAAATGATGTTCTGGTTGCATCACAAGTCAGTAAGAAGGGAAATTCAGATGAGATCCACGAGTTATTAACATGGTTTGATATGAATATTGCTCGATTTGGGGTGCGCAGTGAGTATCTTGAAGATAAGAACAACAGTGACATCGCTGAACAAATGGAAGTACTCAAGAAGACAGTTGATTCTATTTTATCAGAATTGGGGGATCTACGGGCTGCTGCTCAAAGCAAAGATATATTGTTGCAAGAAGAAAGGACTAAGGTAAAAGAATTAACACGCAAGGGTCAGACTCTTGAGAAGTGTTTGCGGGAGAAGGAATCACAATTAAATCTGCTTGAAGGTGTGGAAGACGGCCAGGCAACTAGCTTGACCTCGGAGATTCATGAAGTTGAGCCTGCGATAAACAAATGGGCAGCATCTGGTTCCTCCCTTGCATCGCAAGTCCGCAGTTTGCGCAAAGGCAATAGTGAGCAAGTTGCAATTGCCATAGATATGGATCCTGGGAGCACTAGTAGATTGGAAGATGAAGAAGATGATAAGGTTCATGGTTTCAAGTCGCTCACTACATCAAGAATGGTTCCTAGATTTACAAGACCTCTGACTGACATGGTTGATGGCCTCTGGGTTACTTGTGATAGGACTTTAATGCGACAACCAATTTTGCGGCTTGGTATCATATTCTATTGGGCTTTTTTGCATACGCTTCTTGCTAGTCTTGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2286

Amino Acids

256.25

Weight (kDa)

4.63

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 6245, 6590
AccBSI CCGCTC 1 cut(s) 2878
AccI GTMKAC 3 cut(s) 744, 2126, 4310
AciI CCGC 7 cut(s) 2609, 2878, 5421, 6059, 6442, 6580, 6799
AclI AACGTT 1 cut(s) 4240
AcoI YGGCCR 2 cut(s) 2061, 6487
AdeI CACNNNGTG 1 cut(s) 6135
AfeI AGCGCT 1 cut(s) 2287
AfiI CCNNNNNNNGG 7 cut(s) 1443, 3430, 3866, 4324, 4674, 5427, 6058
AflII CTTAAG 3 cut(s) 2852, 3715, 6115
AflIII ACRYGT 3 cut(s) 1423, 1654, 2209
AhdI GACNNNNNGTC 1 cut(s) 3415
AhlI ACTAGT 4 cut(s) 755, 1673, 5321, 6644
AjiI CACGTC 2 cut(s) 2417, 2467
AjnI CCWGG 6 cut(s) 3155, 3948, 4318, 4623, 6489, 6634
AjuI GAANNNNNNNTTGG 6 cut(s) 3169, 3201, 3245, 3277, 3965, 3997
Alw21I GWGCWC 5 cut(s) 1284, 4932, 5085, 6140, 6644
Alw44I GTGCAC 2 cut(s) 4928, 6136
AlwNI CAGNNNCTG 3 cut(s) 2809, 4136, 6554
Ama87I CYCGRG 1 cut(s) 654
Aor51HI AGCGCT 1 cut(s) 2287
AoxI GGCC 7 cut(s) 435, 1236, 1434, 1467, 2061, 6487, 6751
ApaLI GTGCAC 2 cut(s) 4928, 6136
ArsI GACNNNNNNTTYG 2 cut(s) 2946, 2978
AseI ATTAAT 3 cut(s) 69, 537, 1125
Asp700I GAANNNNTTC 3 cut(s) 1755, 2619, 4808
AspLEI GCGC 7 cut(s) 1027, 1515, 1797, 2288, 5017, 6246, 6591
AspS9I GGNCC 6 cut(s) 185, 436, 773, 1288, 1519, 5674
AsuNHI GCTAGC 2 cut(s) 2282, 4633
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 5 cut(s) 185, 773, 1288, 1519, 5674
AxyI CCTNAGG 1 cut(s) 3850
BaeGI GKGCMC 4 cut(s) 368, 789, 4932, 6140
BalI TGGCCA 1 cut(s) 2063
BamHI GGATCC 1 cut(s) 6631
BanII GRGCYC 2 cut(s) 1284, 5085
BauI CACGAG 3 cut(s) 4467, 5979, 6197
BbsI GAAGAC 6 cut(s) 2679, 2997, 3414, 3486, 6314, 6489
Bbv12I GWGCWC 5 cut(s) 1284, 4932, 5085, 6140, 6644
BbvCI CCTCAGC 3 cut(s) 778, 927, 4686
BceAI ACGGC 1 cut(s) 6502
BciT130I CCWGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
BciVI GTATCC 1 cut(s) 5170
BclI TGATCA 1 cut(s) 3577
BcuI ACTAGT 4 cut(s) 755, 1673, 5321, 6644
BfmI CTRYAG 9 cut(s) 642, 729, 741, 1875, 2127, 2336, 3632, 4941, 6074
BfoI RGCGCY 2 cut(s) 1798, 2289
BfrI CTTAAG 3 cut(s) 2852, 3715, 6115
BfuI GTATCC 1 cut(s) 5170
BglII AGATCT 4 cut(s) 1734, 1954, 2982, 5386
BlpI GCTNAGC 1 cut(s) 2474
BmcAI AGTACT 4 cut(s) 322, 910, 3776, 6300
Bme1390I CCNGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
Bme18I GGWCC 5 cut(s) 185, 773, 1288, 1519, 5674
BmeRI GACNNNNNGTC 1 cut(s) 3415
BmeT110I CYCGRG 1 cut(s) 654
BmgBI CACGTC 2 cut(s) 2417, 2467
BmgT120I GGNCC 6 cut(s) 185, 436, 773, 1288, 1519, 5674
BmiI GGNNCC 8 cut(s) 187, 775, 1289, 1520, 3357, 6559, 6633, 6714
BmrFI CCNGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
BmrI ACTGGG 3 cut(s) 333, 921, 1845
BmtI GCTAGC 2 cut(s) 2286, 4637
BmuI ACTGGG 3 cut(s) 333, 921, 1845
BoxI GACNNNNGTC 2 cut(s) 3569, 4893
BpiI GAAGAC 6 cut(s) 2679, 2997, 3414, 3486, 6314, 6489
BplI GAGNNNNNCTC 6 cut(s) 1424, 1456, 4211, 4243, 5432, 5464
BpmI CTGGAG 9 cut(s) 119, 408, 596, 996, 2546, 3407, 3581, 3763, 4607
Bpu10I CCTNAGC 4 cut(s) 778, 927, 1283, 4686
Bpu1102I GCTNAGC 1 cut(s) 2474
BpuEI CTTGAG 9 cut(s) 1811, 2855, 3527, 3638, 4378, 5242, 5771, 6287, 6449
BsaBI GATNNNNATC 4 cut(s) 1704, 3093, 4032, 6630
BsaI GGTCTC 3 cut(s) 3760, 3890, 4625
BsaWI WCCGGW 1 cut(s) 1819
BsaXI ACNNNNNCTCC 8 cut(s) 4112, 4142, 5702, 5732, 5771, 5801, 6631, 6661
Bsc4I CCNNNNNNNGG 7 cut(s) 1443, 3430, 3866, 4324, 4674, 5427, 6058
Bse118I RCCGGY 1 cut(s) 3110
Bse21I CCTNAGG 1 cut(s) 3850
Bse3DI GCAATG 1 cut(s) 2709
Bse8I GATNNNNATC 4 cut(s) 1704, 3093, 4032, 6630
BseBI CCWGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
BseGI GGATG 9 cut(s) 74, 474, 542, 1062, 1130, 3994, 4033, 5833, 6103
BseJI GATNNNNATC 4 cut(s) 1704, 3093, 4032, 6630
BseLI CCNNNNNNNGG 7 cut(s) 1443, 3430, 3866, 4324, 4674, 5427, 6058
BseMI GCAATG 1 cut(s) 2709
BseSI GKGCMC 4 cut(s) 368, 789, 4932, 6140
BsgI GTGCAG 4 cut(s) 2489, 2762, 3422, 3894
BshFI GGCC 7 cut(s) 437, 1238, 1436, 1469, 2063, 6489, 6753
BsiHKAI GWGCWC 5 cut(s) 1284, 4932, 5085, 6140, 6644
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 2 cut(s) 1820, 3111
BslFI GGGAC 4 cut(s) 171, 340, 759, 928
BslI CCNNNNNNNGG 7 cut(s) 1443, 3430, 3866, 4324, 4674, 5427, 6058
BsmBI CGTCTC 1 cut(s) 657
BsmFI GGGAC 4 cut(s) 171, 340, 759, 928
BsmI GAATGC 1 cut(s) 1201
BsnI GGCC 7 cut(s) 437, 1238, 1436, 1469, 2063, 6489, 6753
Bso31I GGTCTC 3 cut(s) 3760, 3890, 4625
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 7 cut(s) 368, 789, 1284, 4932, 5085, 6140, 6644
Bsp1720I GCTNAGC 1 cut(s) 2474
BspACI CCGC 7 cut(s) 2609, 2878, 5421, 6059, 6442, 6580, 6799
BspANI GGCC 7 cut(s) 437, 1238, 1436, 1469, 2063, 6489, 6753
BspHI TCATGA 3 cut(s) 1888, 5085, 6517
BspLI GGNNCC 8 cut(s) 187, 775, 1289, 1520, 3357, 6559, 6633, 6714
BspMAI CTGCAG 4 cut(s) 2340, 3636, 4945, 6078
BspOI GCTAGC 2 cut(s) 2286, 4637
BspQI GCTCTTC 1 cut(s) 4623
BspTI CTTAAG 3 cut(s) 2852, 3715, 6115
BspTNI GGTCTC 3 cut(s) 3760, 3890, 4625
BsrBI CCGCTC 1 cut(s) 2878
BsrDI GCAATG 1 cut(s) 2709
BsrFI RCCGGY 1 cut(s) 3110
BssAI RCCGGY 1 cut(s) 3110
BssSI CACGAG 3 cut(s) 4467, 5979, 6197
BssT1I CCWWGG 5 cut(s) 161, 2721, 3747, 4668, 5694
Bst2BI CACGAG 3 cut(s) 4467, 5979, 6197
Bst2UI CCWGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
Bst6I CTCTTC 6 cut(s) 301, 1089, 2973, 3095, 4540, 4623
BstAFI CTTAAG 3 cut(s) 2852, 3715, 6115
BstAPI GCANNNNNTGC 4 cut(s) 3798, 4105, 4390, 4643
BstF5I GGATG 9 cut(s) 74, 474, 542, 1062, 1130, 3994, 4033, 5833, 6103
BstH2I RGCGCY 2 cut(s) 1798, 2289
BstHHI GCGC 7 cut(s) 1027, 1515, 1797, 2288, 5017, 6246, 6591
BstNI CCWGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
BstNSI RCATGY 8 cut(s) 446, 1034, 1427, 1658, 2213, 2384, 3334, 5319
BstPAI GACNNNNGTC 2 cut(s) 3569, 4893
BstSCI CCNGG 6 cut(s) 3155, 3948, 4318, 4623, 6489, 6634
BstSFI CTRYAG 9 cut(s) 642, 729, 741, 1875, 2127, 2336, 3632, 4941, 6074
BstSLI GKGCMC 4 cut(s) 368, 789, 4932, 6140
BstV2I GAAGAC 6 cut(s) 2679, 2997, 3414, 3486, 6314, 6489
BstX2I RGATCY 9 cut(s) 1734, 1954, 2498, 2982, 4678, 5386, 6192, 6340, 6631
BstXI CCANNNNNNTGG 2 cut(s) 5170, 6628
BstYI RGATCY 9 cut(s) 1734, 1954, 2498, 2982, 4678, 5386, 6192, 6340, 6631
Bsu36I CCTNAGG 1 cut(s) 3850
BsuI GTATCC 1 cut(s) 5170
BsuRI GGCC 7 cut(s) 437, 1238, 1436, 1469, 2063, 6489, 6753
BtgZI GCGATG 3 cut(s) 5741, 6265, 6555
BtrI CACGTC 2 cut(s) 2417, 2467
BtsCI GGATG 9 cut(s) 74, 474, 542, 1062, 1130, 3994, 4033, 5833, 6103
BtsI GCAGTG 6 cut(s) 201, 789, 4731, 4938, 5161, 6253
CaiI CAGNNNCTG 3 cut(s) 2809, 4136, 6554
CciI TCATGA 3 cut(s) 1888, 5085, 6517
CfoI GCGC 7 cut(s) 1027, 1515, 1797, 2288, 5017, 6246, 6591
Cfr10I RCCGGY 1 cut(s) 3110
Cfr13I GGNCC 6 cut(s) 185, 436, 773, 1288, 1519, 5674
DraIII CACNNNGTG 1 cut(s) 6135
DriI GACNNNNNGTC 1 cut(s) 3415
EaeI YGGCCR 2 cut(s) 2061, 6487
Eam1104I CTCTTC 6 cut(s) 301, 1089, 2973, 3095, 4540, 4623
Eam1105I GACNNNNNGTC 1 cut(s) 3415
EarI CTCTTC 6 cut(s) 301, 1089, 2973, 3095, 4540, 4623
EciI GGCGGA 1 cut(s) 2624
Ecl136II GAGCTC 2 cut(s) 1282, 5083
Eco130I CCWWGG 5 cut(s) 161, 2721, 3747, 4668, 5694
Eco147I AGGCCT 2 cut(s) 1238, 1469
Eco24I GRGCYC 2 cut(s) 1284, 5085
Eco31I GGTCTC 3 cut(s) 3760, 3890, 4625
Eco32I GATATC 2 cut(s) 3199, 4034
Eco47I GGWCC 5 cut(s) 185, 773, 1288, 1519, 5674
Eco47III AGCGCT 1 cut(s) 2287
Eco53kI GAGCTC 2 cut(s) 1282, 5083
Eco81I CCTNAGG 1 cut(s) 3850
Eco88I CYCGRG 1 cut(s) 654
EcoICRI GAGCTC 2 cut(s) 1282, 5083
EcoO109I RGGNCCY 2 cut(s) 1288, 1519
EcoRI GAATTC 4 cut(s) 736, 991, 1177, 1804
EcoRII CCWGG 6 cut(s) 3155, 3948, 4318, 4623, 6489, 6634
EcoRV GATATC 2 cut(s) 3199, 4034
EcoT14I CCWWGG 5 cut(s) 161, 2721, 3747, 4668, 5694
EcoT22I ATGCAT 2 cut(s) 3678, 5901
EcoT38I GRGCYC 2 cut(s) 1284, 5085
ErhI CCWWGG 5 cut(s) 161, 2721, 3747, 4668, 5694
Esp3I CGTCTC 1 cut(s) 657
FalI AAGNNNNNCTT 4 cut(s) 2607, 2639, 4092, 4124
FaqI GGGAC 4 cut(s) 171, 340, 759, 928
FauI CCCGC 3 cut(s) 2871, 5428, 6435
FbaI TGATCA 1 cut(s) 3577
FblI GTMKAC 3 cut(s) 744, 2126, 4310
FokI GGATG 9 cut(s) 61, 481, 529, 1069, 1117, 4001, 4040, 5840, 6110
FriOI GRGCYC 2 cut(s) 1284, 5085
FspI TGCGCA 2 cut(s) 6245, 6590
GlaI GCGC 7 cut(s) 1026, 1514, 1796, 2287, 5016, 6245, 6590
GsuI CTGGAG 9 cut(s) 119, 408, 596, 996, 2546, 3407, 3581, 3763, 4607
HaeII RGCGCY 2 cut(s) 1798, 2289
HaeIII GGCC 7 cut(s) 437, 1238, 1436, 1469, 2063, 6489, 6753
HapII CCGG 2 cut(s) 1820, 3111
HhaI GCGC 7 cut(s) 1027, 1515, 1797, 2288, 5017, 6246, 6591
Hin6I GCGC 7 cut(s) 1025, 1513, 1795, 2286, 5015, 6244, 6589
HinP1I GCGC 7 cut(s) 1025, 1513, 1795, 2286, 5015, 6244, 6589
HincII GTYRAC 7 cut(s) 709, 1411, 1642, 1867, 2254, 3439, 4225
HindII GTYRAC 7 cut(s) 709, 1411, 1642, 1867, 2254, 3439, 4225
HpaI GTTAAC 2 cut(s) 2254, 3439
HpaII CCGG 2 cut(s) 1820, 3111
Hpy99I CGWCG 1 cut(s) 4264
HpyCH4IV ACGT 6 cut(s) 1401, 1632, 2416, 2466, 4240, 5595
HpySE526I ACGT 6 cut(s) 1401, 1632, 2416, 2466, 4240, 5595
HspAI GCGC 7 cut(s) 1025, 1513, 1795, 2286, 5015, 6244, 6589
Ksp22I TGATCA 1 cut(s) 3577
KspAI GTTAAC 2 cut(s) 2254, 3439
LguI GCTCTTC 1 cut(s) 4623
LmnI GCTCC 6 cut(s) 2512, 3355, 5620, 5767, 5860, 6639
MaeII ACGT 6 cut(s) 1401, 1632, 2416, 2466, 4240, 5595
MbiI CCGCTC 1 cut(s) 2878
MfeI CAATTG 5 cut(s) 1691, 2393, 3373, 5175, 6615
MflI RGATCY 9 cut(s) 1734, 1954, 2498, 2982, 4678, 5386, 6192, 6340, 6631
MhlI GDGCHC 7 cut(s) 368, 789, 1284, 4932, 5085, 6140, 6644
MlsI TGGCCA 1 cut(s) 2063
MluNI TGGCCA 1 cut(s) 2063
MmeI TCCRAC 7 cut(s) 1728, 3243, 3898, 3963, 4467, 5362, 5700
Mox20I TGGCCA 1 cut(s) 2063
Mph1103I ATGCAT 2 cut(s) 3678, 5901
MroXI GAANNNNTTC 3 cut(s) 1755, 2619, 4808
MscI TGGCCA 1 cut(s) 2063
MslI CAYNNNNRTG 9 cut(s) 1230, 1428, 1659, 2571, 3681, 3870, 5028, 5292, 6626
Msp20I TGGCCA 1 cut(s) 2063
MspA1I CMGCKG 4 cut(s) 1772, 3878, 4946, 5519
MspCI CTTAAG 3 cut(s) 2852, 3715, 6115
MspI CCGG 2 cut(s) 1820, 3111
MspR9I CCNGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
MunI CAATTG 5 cut(s) 1691, 2393, 3373, 5175, 6615
Mva1269I GAATGC 1 cut(s) 1201
MvaI CCWGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
NheI GCTAGC 2 cut(s) 2282, 4633
NlaIV GGNNCC 8 cut(s) 187, 775, 1289, 1520, 3357, 6559, 6633, 6714
NmeAIII GCCGAG 1 cut(s) 170
NsbI TGCGCA 2 cut(s) 6245, 6590
NsiI ATGCAT 2 cut(s) 3678, 5901
NspI RCATGY 8 cut(s) 446, 1034, 1427, 1658, 2213, 2384, 3334, 5319
PaeI GCATGC 1 cut(s) 5319
PagI TCATGA 3 cut(s) 1888, 5085, 6517
PceI AGGCCT 2 cut(s) 1238, 1469
PciI ACATGT 3 cut(s) 1423, 1654, 2209
PciSI GCTCTTC 1 cut(s) 4623
PctI GAATGC 1 cut(s) 1201
PdmI GAANNNNTTC 3 cut(s) 1755, 2619, 4808
PfoI TCCNGGA 2 cut(s) 3155, 4623
PpuMI RGGWCCY 2 cut(s) 1288, 1519
PscI ACATGT 3 cut(s) 1423, 1654, 2209
PshAI GACNNNNGTC 2 cut(s) 3569, 4893
PshBI ATTAAT 3 cut(s) 69, 537, 1125
Psp124BI GAGCTC 2 cut(s) 1284, 5085
Psp1406I AACGTT 1 cut(s) 4240
Psp5II RGGWCCY 2 cut(s) 1288, 1519
Psp6I CCWGG 6 cut(s) 3155, 3948, 4318, 4623, 6489, 6634
PspGI CCWGG 6 cut(s) 3155, 3948, 4318, 4623, 6489, 6634
PspN4I GGNNCC 8 cut(s) 187, 775, 1289, 1520, 3357, 6559, 6633, 6714
PspPI GGNCC 6 cut(s) 185, 436, 773, 1288, 1519, 5674
PspPPI RGGWCCY 2 cut(s) 1288, 1519
PsrI GAACNNNNNNTAC 2 cut(s) 5730, 5762
PstI CTGCAG 4 cut(s) 2340, 3636, 4945, 6078
PstNI CAGNNNCTG 3 cut(s) 2809, 4136, 6554
PsuI RGATCY 9 cut(s) 1734, 1954, 2498, 2982, 4678, 5386, 6192, 6340, 6631
PvuII CAGCTG 4 cut(s) 1772, 3878, 4946, 5519
RseI CAYNNNNRTG 9 cut(s) 1230, 1428, 1659, 2571, 3681, 3870, 5028, 5292, 6626
SacI GAGCTC 2 cut(s) 1284, 5085
SapI GCTCTTC 1 cut(s) 4623
Sau96I GGNCC 6 cut(s) 185, 436, 773, 1288, 1519, 5674
ScaI AGTACT 4 cut(s) 322, 910, 3776, 6300
ScrFI CCNGG 6 cut(s) 3157, 3950, 4320, 4625, 6491, 6636
SduI GDGCHC 7 cut(s) 368, 789, 1284, 4932, 5085, 6140, 6644
SfcI CTRYAG 9 cut(s) 642, 729, 741, 1875, 2127, 2336, 3632, 4941, 6074
SinI GGWCC 5 cut(s) 185, 773, 1288, 1519, 5674
SmiMI CAYNNNNRTG 9 cut(s) 1230, 1428, 1659, 2571, 3681, 3870, 5028, 5292, 6626
SpeI ACTAGT 4 cut(s) 755, 1673, 5321, 6644
SphI GCATGC 1 cut(s) 5319
SseBI AGGCCT 2 cut(s) 1238, 1469
SsiI CCGC 7 cut(s) 2609, 2878, 5421, 6059, 6442, 6580, 6799
SspI AATATT 5 cut(s) 67, 535, 1045, 1123, 6226
SstI GAGCTC 2 cut(s) 1284, 5085
StuI AGGCCT 2 cut(s) 1238, 1469
StyD4I CCNGG 6 cut(s) 3155, 3948, 4318, 4623, 6489, 6634
StyI CCWWGG 5 cut(s) 161, 2721, 3747, 4668, 5694
TaiI ACGT 6 cut(s) 1404, 1635, 2419, 2469, 4243, 5598
TaqI TCGA 6 cut(s) 453, 1349, 1580, 3923, 5046, 6232
TauI GCSGC 1 cut(s) 6802
TspGWI ACGGA 5 cut(s) 463, 681, 1051, 5105, 5840
Vha464I CTTAAG 3 cut(s) 2852, 3715, 6115
VneI GTGCAC 2 cut(s) 4928, 6136
VpaK11BI GGWCC 5 cut(s) 185, 773, 1288, 1519, 5674
VspI ATTAAT 3 cut(s) 69, 537, 1125
XbaI TCTAGA 2 cut(s) 2176, 4036
XceI RCATGY 8 cut(s) 446, 1034, 1427, 1658, 2213, 2384, 3334, 5319
XmiI GTMKAC 3 cut(s) 744, 2126, 4310
XmnI GAANNNNTTC 3 cut(s) 1755, 2619, 4808
ZrmI AGTACT 4 cut(s) 322, 910, 3776, 6300
Zsp2I ATGCAT 2 cut(s) 3678, 5901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.