Rorug06G0337500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001092
Physical Location & Seq
Forward (+)
48968252 .. 48969012
761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0337500.1

Sequence Viewer

Length: 426 bp
ATGACCACCGCCGTCGCCACAACCGCTCAGGCCGGCCGAGGCAAGCCCAAGACCACCAAATCCGTCTCCAGGTCATCCAAGGCCGGCCTCCAATTTCCCGTCGGCCGAGTCGCCCGCTTCCTCAAGAAGGGCCGCTACGCCCAGCGTGTCGGCGGTGGCTCCCCCGTCTACCTCTCAGCCGTCCTCGAATACCTAGCTGCTGAGGTTTTGGAGCTGGCTGGAAACGCTGCCAGGGACAACAAGAAGAATCGGATCACTCCAAGGCATATCCAGCTTGCGGTGAGGAATGATGAGGAGTTCACCAAGCTTTTGGGGTCTGTGACGATTGCGAATGGAGGTGTTCTGCCCAACATTCATCAGAACTTGCTGCCCAAGAAGAATGCGAAAGGGAAGGGCGAGATTGGGTCTATGTCTCAGGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.07

Weight (kDa)

10.58

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 17 - 95 5e-15 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 98 - 131 2.1e-17 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 168
AciI CCGC 6 cut(s) 9, 24, 115, 133, 153, 278
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 2 cut(s) 34, 103
AcsI RAATTY 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 69, 127, 277
AjnI CCWGG 2 cut(s) 68, 230
AluBI AGCT 4 cut(s) 197, 214, 274, 307
AluI AGCT 4 cut(s) 197, 214, 274, 307
Alw26I GTCTC 2 cut(s) 70, 417
AlwI GGATC 1 cut(s) 260
AoxI GGCC 6 cut(s) 30, 34, 81, 85, 103, 130
ApeKI GCWGC 3 cut(s) 197, 227, 367
ApoI RAATTY 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 292, 292
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 3 cut(s) 184, 214, 354
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 70, 232
BcoDI GTCTC 2 cut(s) 70, 417
BfaI CTAG 2 cut(s) 194, 424
BisI GCNGC 4 cut(s) 133, 198, 228, 368
BlsI GCNGC 4 cut(s) 134, 199, 229, 369
Bme1390I CCNGG 2 cut(s) 70, 232
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 70, 232
BpmI CTGGAG 1 cut(s) 52
Bpu10I CCTNAGC 2 cut(s) 27, 201
BpuEI CTTGAG 1 cut(s) 107
BsaJI CCNNGG 4 cut(s) 37, 78, 231, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 127, 277
Bse118I RCCGGY 2 cut(s) 32, 83
BseBI CCWGG 2 cut(s) 70, 232
BseDI CCNNGG 4 cut(s) 37, 78, 231, 260
BseGI GGATG 1 cut(s) 74
BseLI CCNNNNNNNGG 3 cut(s) 69, 127, 277
BseMII CTCAG 3 cut(s) 41, 189, 192
BseRI GAGGAG 1 cut(s) 308
BseX3I CGGCCG 2 cut(s) 34, 103
BseXI GCAGC 3 cut(s) 184, 214, 354
BseYI CCCAGC 1 cut(s) 141
Bsh1285I CGRYCG 2 cut(s) 37, 106
BshFI GGCC 6 cut(s) 32, 36, 83, 87, 105, 132
BsiEI CGRYCG 2 cut(s) 37, 106
BsiSI CCGG 2 cut(s) 33, 84
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 69, 127, 277
BsmAI GTCTC 2 cut(s) 70, 417
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 6 cut(s) 32, 36, 83, 87, 105, 132
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 6 cut(s) 9, 24, 115, 133, 153, 278
BspANI GGCC 6 cut(s) 32, 36, 83, 87, 105, 132
BspCNI CTCAG 3 cut(s) 40, 188, 193
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 26
BsrFI RCCGGY 2 cut(s) 32, 83
BssAI RCCGGY 2 cut(s) 32, 83
BssECI CCNNGG 4 cut(s) 37, 78, 231, 260
BssMI GATC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 78, 260
Bst2UI CCWGG 2 cut(s) 70, 232
BstC8I GCNNGC 6 cut(s) 34, 44, 85, 115, 216, 276
BstDEI CTNAG 4 cut(s) 27, 175, 201, 414
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 74
BstKTI GATC 1 cut(s) 255
BstMAI GTCTC 2 cut(s) 70, 417
BstMBI GATC 1 cut(s) 252
BstMCI CGRYCG 2 cut(s) 37, 106
BstMWI GCNNNNNNNGC 3 cut(s) 23, 224, 271
BstNI CCWGG 2 cut(s) 70, 232
BstSCI CCNGG 2 cut(s) 68, 230
BstV1I GCAGC 3 cut(s) 184, 214, 354
BstXI CCANNNNNNTGG 1 cut(s) 310
BstZI CGGCCG 2 cut(s) 34, 103
BsuRI GGCC 6 cut(s) 32, 36, 83, 87, 105, 132
BtsCI GGATG 1 cut(s) 74
Cac8I GCNNGC 6 cut(s) 34, 44, 85, 115, 216, 276
Cfr10I RCCGGY 2 cut(s) 32, 83
Cfr13I GGNCC 1 cut(s) 130
DdeI CTNAG 4 cut(s) 27, 175, 201, 414
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
EaeI YGGCCR 2 cut(s) 34, 103
EagI CGGCCG 2 cut(s) 34, 103
EclXI CGGCCG 2 cut(s) 34, 103
Eco130I CCWWGG 2 cut(s) 78, 260
Eco52I CGGCCG 2 cut(s) 34, 103
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 68, 230
EcoT14I CCWWGG 2 cut(s) 78, 260
ErhI CCWWGG 2 cut(s) 78, 260
Esp3I CGTCTC 1 cut(s) 70
FaiI YATR 2 cut(s) 267, 410
FaqI GGGAC 1 cut(s) 248
FauI CCCGC 1 cut(s) 122
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 4 cut(s) 133, 198, 228, 368
FokI GGATG 1 cut(s) 61
FseI GGCCGGCC 2 cut(s) 36, 87
Fsp4HI GCNGC 4 cut(s) 133, 198, 228, 368
FspBI CTAG 2 cut(s) 194, 424
GluI GCNGC 4 cut(s) 133, 198, 228, 368
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 52
HaeIII GGCC 6 cut(s) 32, 36, 83, 87, 105, 132
HapII CCGG 2 cut(s) 33, 84
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 2 cut(s) 108, 247
HpaII CCGG 2 cut(s) 33, 84
HphI GGTGA 2 cut(s) 292, 292
Hpy166II GTNNAC 2 cut(s) 169, 300
Hpy188I TCNGA 2 cut(s) 252, 360
Hpy188III TCNNGA 2 cut(s) 124, 416
Hpy8I GTNNAC 2 cut(s) 169, 300
Hpy99I CGWCG 2 cut(s) 17, 104
HpyAV CCTTC 2 cut(s) 121, 385
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 224, 271
HpyF3I CTNAG 4 cut(s) 27, 175, 201, 414
KroI GCCGGC 2 cut(s) 32, 83
KroNI GCCGGC 2 cut(s) 34, 85
Kzo9I GATC 1 cut(s) 252
LmnI GCTCC 2 cut(s) 164, 211
Lsp1109I GCAGC 3 cut(s) 184, 214, 354
MaeI CTAG 2 cut(s) 194, 424
MaeIII GTNAC 1 cut(s) 319
MalI GATC 1 cut(s) 254
MbiI CCGCTC 1 cut(s) 26
MboI GATC 1 cut(s) 252
MboII GAAGA 2 cut(s) 256, 388
MluCI AATT 2 cut(s) 92, 419
MlyI GAGTC 1 cut(s) 117
MnlI CCTC 9 cut(s) 32, 98, 131, 182, 194, 196, 276, 286, 329
MroNI GCCGGC 2 cut(s) 32, 83
MspI CCGG 2 cut(s) 33, 84
MspR9I CCNGG 2 cut(s) 70, 232
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 2 cut(s) 70, 232
MwoI GCNNNNNNNGC 3 cut(s) 23, 224, 271
NaeI GCCGGC 2 cut(s) 34, 85
NdeII GATC 1 cut(s) 252
NgoMIV GCCGGC 2 cut(s) 32, 83
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 2 cut(s) 62, 131
NmuCI GTSAC 1 cut(s) 319
PctI GAATGC 1 cut(s) 385
PdiI GCCGGC 2 cut(s) 34, 85
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 4 cut(s) 134, 199, 229, 369
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Psp6I CCWGG 2 cut(s) 68, 230
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 2 cut(s) 68, 230
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 130
RigI GGCCGGCC 2 cut(s) 36, 87
SatI GCNGC 4 cut(s) 133, 198, 228, 368
Sau3AI GATC 1 cut(s) 252
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 117
ScrFI CCNGG 2 cut(s) 70, 232
SetI ASST 9 cut(s) 74, 174, 195, 199, 207, 216, 276, 309, 340
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 92, 419
SsiI CCGC 6 cut(s) 9, 24, 115, 133, 153, 278
SspMI CTAG 2 cut(s) 194, 424
StyD4I CCNGG 2 cut(s) 68, 230
StyI CCWWGG 2 cut(s) 78, 260
TaqI TCGA 1 cut(s) 186
TasI AATT 2 cut(s) 92, 419
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 247
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 197, 227, 367
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 2 cut(s) 194, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.