Rh6DG449900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001092
Physical Location & Seq
Forward (+)
62240775 .. 62244894
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG449900.1

Sequence Viewer

Length: 1743 bp
ATGTGCTTTGATTTACGCACAGATGTAGCTTCTTCTGAGCAAGAGACTAGGAAATCCAAAAGAGCAGCAGAGCTACTCGTTGCAGAACTGAATGAGGTTCAAGAGAGGAATGACGGTCTCCAGGAAGAGCTAGCAAAATCTGCTGATGAAATTTCTATACTCTCCAAGGAAAGGGATCTTGCTGAGGCTGGCAAGCTTGAAGCTGTTTTAAGTCTTGAAAAGTTATCTACTGCTCACTCCGAGGAAAGAAACCAATTTTCTGAATTTGCAGGACTGAAATCTGGTGTAGATCAACTCAGGAAGGGTTTCCATGATATTAGCAATTCATTGGCTGGTCTTTTTTACAACGACTTGGAATTTTTGAAAAACTTGGAGTCTGGTATTGACTCATGTCTCAACTTGAATAGTGCTAATGTGGTTGATGTGCACCCCTTCACTGCAGCTGGTGGATTTCTATTGAGCAAATCAGATAAGGTTAACTCTATTTCAATTAATTCTTGGTCAGACCCCGAGACGCATGGACATTTTGGTGACAGTTTTGCCATCGAAACCTTTACATACATAACACATTATGTGCAAGAGCTCATGACGGAGATTGGTGTGCTTAAAGAAAAATTAGATGAACACTCAATGTCTTTGCATGAAAAATCTAGCAGTGTATCCAGATTGGTGGCAATTGTTCGTGGAGAGATAAGTTCCAAAAATGAGTCGTTTGAAGCCTTGAGGAGAGACTTTTTGCAGATGGAAATGGTCAAAAAGGAAAAGGACAAGGAACTTCTTGTCTTGCATAGAAATGCTGCCTTGCTTTTTGAAGCATGCACTAGTTCTGTTGTGGAAATAAATAGAAGAAAAGCTGAATTGGTGGGAAATAGTTGGGTTGTTGGAGATCTGGGAATGACATCAAAAATAGCAGAATTGCCCGCTTTCAGGGGAGAGGGTGAGCTGTACTCTGAGGAATCTGTTAGGTCTGTGGCAGAGAGGCTTTTGTCGGCTGCTAGTGATTTTGCTACTCTGACAGCTGAAATAGTTGAAGGCAGCCAAAAGGAAATGAAGCTTACCATTTCAAATTTGCAGAAAGAGCTTCAGGAGAAGGGCGTTCAAAAGGAGAGGATTTTCATGGAGCTTGTAAGTCAAATCAAGGAGGCTGAAGCTACTGCAAGCAGCTATTCAGTGGACCTTGAATCTTCAAAAACCTTGGTGCATGATTTGGAGAAACAGTTGGAAGCAATGAAAGGTGAACGCAACCTACTTGAGCAGAGAGTAAAGGAGCTAGAAGATGGCCATGCCTCCTCTGACGAGTTACAACAGAGGGTCAGATCACTAATGGATGTGCTTGCTGCCAAAGACCGTGAAATTGAGGAGCTAATCCAAGCACTTGATGAGGAGGAGGTACAGATGCAAGGTATCACCTTCAAGATCAAGGAACTGGAAAAGATCGTTGAACAAAAAAACCTAGATTTAGAGAACCTTGAAGCTTCTCGTGGCAAGGTGATGAAAAAGCTCTTCGTCACTGTGAATAAGTTTGACGAGCTGCATCATCTATCTGCAAGCCTCCTTGCGGAAGTTGAAGAACTGCAGTCACAGTTGCAAGATCGGGATGCAGAGATTTCTTTCTTAAGGCAAGAGGTCACTAGGTGCACCAATGATGTTCTGGTTGCATCACAAGCAACTCAGATTCCCATATTTTTATCTGAAGGAATCCTAGAAGCTTGGTTGCATCTAATAAGGAAAAGAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

580

Amino Acids

65.23

Weight (kDa)

4.89

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HOOK PF05622 334 - 545 1.2e-06 HOOK protein coiled-coil region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 921, 1559
AclWI GGATC 1 cut(s) 183
AcoI YGGCCR 1 cut(s) 1279
AcsI RAATTY 4 cut(s) 150, 263, 356, 1066
AcuI CTGAAG 3 cut(s) 1067, 1167, 1713
AdeI CACNNNGTG 1 cut(s) 1635
AfaI GTAC 2 cut(s) 947, 1392
AfiI CCNNNNNNNGG 3 cut(s) 171, 927, 1558
AflII CTTAAG 1 cut(s) 1615
AhlI ACTAGT 1 cut(s) 821
AjnI CCWGG 1 cut(s) 120
Alw21I GWGCWC 3 cut(s) 429, 585, 1640
Alw26I GTCTC 5 cut(s) 38, 122, 398, 506, 723
Alw44I GTGCAC 2 cut(s) 425, 1636
AlwI GGATC 1 cut(s) 183
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 1 cut(s) 1279
ApaLI GTGCAC 2 cut(s) 425, 1636
ApeKI GCWGC 8 cut(s) 65, 440, 797, 992, 1035, 1161, 1337, 1531
ApoI RAATTY 4 cut(s) 150, 263, 356, 1066
AseI ATTAAT 1 cut(s) 492
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 1174
AsuHPI GGTGA 5 cut(s) 542, 950, 1247, 1399, 1501
AsuNHI GCTAGC 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 1174
BaeGI GKGCMC 2 cut(s) 429, 1640
BalI TGGCCA 1 cut(s) 1281
BanII GRGCYC 1 cut(s) 585
BauI CACGAG 1 cut(s) 1479
Bbv12I GWGCWC 3 cut(s) 429, 585, 1640
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 8 cut(s) 77, 452, 784, 979, 1047, 1173, 1324, 1518
BccI CCATC 3 cut(s) 551, 736, 1271
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 670
BcoDI GTCTC 5 cut(s) 38, 122, 398, 506, 723
BcuI ACTAGT 1 cut(s) 821
BfaI CTAG 9 cut(s) 48, 131, 651, 822, 996, 1271, 1454, 1632, 1703
BfmI CTRYAG 2 cut(s) 438, 1574
BfrI CTTAAG 1 cut(s) 1615
BfuI GTATCC 1 cut(s) 670
BglII AGATCT 1 cut(s) 886
BisI GCNGC 8 cut(s) 66, 441, 798, 993, 1036, 1162, 1338, 1532
BlsI GCNGC 8 cut(s) 67, 442, 799, 994, 1037, 1163, 1339, 1533
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 1174
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 1174
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 5 cut(s) 1386, 1543, 1588, 1667, 1726
BmtI GCTAGC 1 cut(s) 134
BoxI GACNNNNGTC 1 cut(s) 390
BplI GAGNNNNNCTC 2 cut(s) 932, 964
BpmI CTGGAG 1 cut(s) 104
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 2 cut(s) 742, 1271
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 165, 240, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 1202, 1232
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 927, 1558
Bse1I ACTGG 1 cut(s) 1431
Bse3DI GCAATG 1 cut(s) 1233
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 165, 240, 1194
BseGI GGATG 2 cut(s) 1333, 1603
BseLI CCNNNNNNNGG 3 cut(s) 171, 927, 1558
BseMI GCAATG 1 cut(s) 1233
BseMII CTCAG 5 cut(s) 27, 174, 310, 942, 1685
BseNI ACTGG 1 cut(s) 1431
BseRI GAGGAG 5 cut(s) 739, 1279, 1373, 1397, 1400
BseSI GKGCMC 2 cut(s) 429, 1640
BseXI GCAGC 8 cut(s) 77, 452, 784, 979, 1047, 1173, 1324, 1518
BshFI GGCC 1 cut(s) 1281
BsiHKAI GWGCWC 3 cut(s) 429, 585, 1640
BsiHKCI CYCGRG 1 cut(s) 509
BslI CCNNNNNNNGG 3 cut(s) 171, 927, 1558
BsmAI GTCTC 5 cut(s) 38, 122, 398, 506, 723
BsmBI CGTCTC 1 cut(s) 506
BsnI GGCC 1 cut(s) 1281
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 3 cut(s) 429, 585, 1640
Bsp143I GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
BspACI CCGC 2 cut(s) 921, 1559
BspANI GGCC 1 cut(s) 1281
BspCNI CTCAG 5 cut(s) 28, 175, 309, 943, 1684
BspHI TCATGA 2 cut(s) 585, 1739
BspMAI CTGCAG 2 cut(s) 442, 1578
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 1 cut(s) 183
BspQI GCTCTTC 2 cut(s) 120, 1508
BspTI CTTAAG 1 cut(s) 1615
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 1233
BsrI ACTGG 1 cut(s) 1431
BssECI CCNNGG 3 cut(s) 165, 240, 1194
BssMI GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
BssSI CACGAG 1 cut(s) 1479
BssT1I CCWWGG 2 cut(s) 165, 1194
Bst2BI CACGAG 1 cut(s) 1479
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 6 cut(s) 116, 536, 1218, 1349, 1513, 1584
Bst6I CTCTTC 2 cut(s) 120, 1508
BstAFI CTTAAG 1 cut(s) 1615
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 8 cut(s) 132, 190, 194, 817, 921, 1159, 1335, 1549
BstDEI CTNAG 5 cut(s) 36, 183, 296, 951, 1671
BstF5I GGATG 2 cut(s) 1333, 1603
BstKTI GATC 7 cut(s) 178, 292, 889, 1319, 1419, 1437, 1594
BstMAI GTCTC 5 cut(s) 38, 122, 398, 506, 723
BstMBI GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
BstMWI GCNNNNNNNGC 3 cut(s) 140, 1078, 1664
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 819
BstPAI GACNNNNGTC 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 120
BstSFI CTRYAG 2 cut(s) 438, 1574
BstSLI GKGCMC 2 cut(s) 429, 1640
BstV1I GCAGC 8 cut(s) 77, 452, 784, 979, 1047, 1173, 1324, 1518
BstX2I RGATCY 2 cut(s) 175, 886
BstXI CCANNNNNNTGG 1 cut(s) 670
BstYI RGATCY 2 cut(s) 175, 886
BsuI GTATCC 1 cut(s) 670
BsuRI GGCC 1 cut(s) 1281
BtsCI GGATG 2 cut(s) 1333, 1603
BtsI GCAGTG 2 cut(s) 435, 661
BtsIMutI CAGTG 4 cut(s) 435, 661, 1176, 1509
Cac8I GCNNGC 8 cut(s) 132, 190, 194, 817, 921, 1159, 1335, 1549
CciI TCATGA 2 cut(s) 585, 1739
Cfr13I GGNCC 1 cut(s) 1174
CseI GACGC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 946, 1391
CviQI GTAC 2 cut(s) 946, 1391
DdeI CTNAG 5 cut(s) 36, 183, 296, 951, 1671
DpnI GATC 7 cut(s) 177, 291, 888, 1318, 1418, 1436, 1593
DpnII GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
DraIII CACNNNGTG 1 cut(s) 1635
EaeI YGGCCR 1 cut(s) 1279
Eam1104I CTCTTC 2 cut(s) 120, 1508
EarI CTCTTC 2 cut(s) 120, 1508
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 2 cut(s) 165, 1194
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 1174
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 3 cut(s) 1067, 1167, 1713
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 165, 1194
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 2 cut(s) 165, 1194
Esp3I CGTCTC 1 cut(s) 506
FauI CCCGC 1 cut(s) 928
Fnu4HI GCNGC 8 cut(s) 66, 441, 798, 993, 1036, 1162, 1338, 1532
FokI GGATG 2 cut(s) 1340, 1610
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 8 cut(s) 66, 441, 798, 993, 1036, 1162, 1338, 1532
FspBI CTAG 9 cut(s) 48, 131, 651, 822, 996, 1271, 1454, 1632, 1703
GluI GCNGC 8 cut(s) 66, 441, 798, 993, 1036, 1162, 1338, 1532
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 1 cut(s) 1281
HgaI GACGC 1 cut(s) 523
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 4 cut(s) 194, 1052, 1473, 1707
HinfI GANTC 8 cut(s) 374, 386, 707, 956, 1181, 1675, 1698, 1736
HpaI GTTAAC 1 cut(s) 478
HphI GGTGA 5 cut(s) 542, 950, 1247, 1399, 1501
Hpy166II GTNNAC 5 cut(s) 427, 478, 1174, 1238, 1638
Hpy188III TCNNGA 9 cut(s) 101, 215, 298, 586, 663, 1085, 1414, 1595, 1740
Hpy8I GTNNAC 5 cut(s) 427, 478, 1174, 1238, 1638
HpyAV CCTTC 6 cut(s) 295, 442, 1025, 1084, 1420, 1688
HpyCH4III ACNGT 6 cut(s) 116, 536, 1218, 1349, 1513, 1584
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 1078, 1664
HpyF3I CTNAG 5 cut(s) 36, 183, 296, 951, 1671
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
LguI GCTCTTC 2 cut(s) 120, 1508
LmnI GCTCC 3 cut(s) 1120, 1267, 1360
Lsp1109I GCAGC 8 cut(s) 77, 452, 784, 979, 1047, 1173, 1324, 1518
LweI GCATC 5 cut(s) 1386, 1543, 1588, 1667, 1726
MaeI CTAG 9 cut(s) 48, 131, 651, 822, 996, 1271, 1454, 1632, 1703
MaeIII GTNAC 5 cut(s) 530, 1299, 1507, 1578, 1627
MalI GATC 7 cut(s) 177, 291, 888, 1318, 1418, 1436, 1593
MboI GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
MboII GAAGA 7 cut(s) 24, 137, 858, 1176, 1286, 1495, 1580
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 2 cut(s) 175, 886
MhlI GDGCHC 3 cut(s) 429, 585, 1640
MlsI TGGCCA 1 cut(s) 1281
MluNI TGGCCA 1 cut(s) 1281
MlyI GAGTC 3 cut(s) 380, 383, 716
MmeI TCCRAC 2 cut(s) 862, 1200
Mox20I TGGCCA 1 cut(s) 1281
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 1281
MseI TTAA 5 cut(s) 209, 477, 492, 606, 1616
MslI CAYNNNNRTG 2 cut(s) 528, 792
Msp20I TGGCCA 1 cut(s) 1281
MspA1I CMGCKG 2 cut(s) 443, 1019
MspCI CTTAAG 1 cut(s) 1615
MspR9I CCNGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 675
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 140, 1078, 1664
NdeII GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
NheI GCTAGC 1 cut(s) 130
NmuCI GTSAC 4 cut(s) 530, 1507, 1578, 1627
NspI RCATGY 1 cut(s) 819
PaeI GCATGC 1 cut(s) 819
PagI TCATGA 2 cut(s) 585, 1739
PciSI GCTCTTC 2 cut(s) 120, 1508
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 5 cut(s) 956, 1181, 1675, 1698, 1736
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 8 cut(s) 67, 442, 799, 994, 1037, 1163, 1339, 1533
PleI GAGTC 3 cut(s) 380, 382, 715
PpsI GAGTC 3 cut(s) 380, 382, 715
PshAI GACNNNNGTC 1 cut(s) 390
PshBI ATTAAT 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspPI GGNCC 1 cut(s) 1174
PsrI GAACNNNNNNTAC 2 cut(s) 1230, 1262
PstI CTGCAG 2 cut(s) 442, 1578
PsuI RGATCY 2 cut(s) 175, 886
PvuII CAGCTG 2 cut(s) 443, 1019
RsaI GTAC 2 cut(s) 947, 1392
RsaNI GTAC 2 cut(s) 946, 1391
RseI CAYNNNNRTG 2 cut(s) 528, 792
SacI GAGCTC 1 cut(s) 585
SapI GCTCTTC 2 cut(s) 120, 1508
SaqAI TTAA 5 cut(s) 209, 477, 492, 606, 1616
SatI GCNGC 8 cut(s) 66, 441, 798, 993, 1036, 1162, 1338, 1532
Sau3AI GATC 7 cut(s) 175, 289, 886, 1316, 1416, 1434, 1591
Sau96I GGNCC 1 cut(s) 1174
SchI GAGTC 3 cut(s) 380, 383, 716
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 3 cut(s) 429, 585, 1640
SfaNI GCATC 5 cut(s) 1386, 1543, 1588, 1667, 1726
SfcI CTRYAG 2 cut(s) 438, 1574
SinI GGWCC 1 cut(s) 1174
SmiMI CAYNNNNRTG 2 cut(s) 528, 792
SmlI CTYRAG 3 cut(s) 721, 1250, 1615
SmoI CTYRAG 3 cut(s) 721, 1250, 1615
SpeI ACTAGT 1 cut(s) 821
SphI GCATGC 1 cut(s) 819
SsiI CCGC 2 cut(s) 921, 1559
SspMI CTAG 9 cut(s) 48, 131, 651, 822, 996, 1271, 1454, 1632, 1703
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 2 cut(s) 165, 1194
TaaI ACNGT 6 cut(s) 116, 536, 1218, 1349, 1513, 1584
TaqI TCGA 1 cut(s) 546
TatI WGTACW 1 cut(s) 945
TfiI GAWTC 5 cut(s) 956, 1181, 1675, 1698, 1736
Tru1I TTAA 5 cut(s) 209, 477, 492, 606, 1616
Tru9I TTAA 5 cut(s) 209, 477, 492, 606, 1616
TscAI CASTG 4 cut(s) 442, 661, 1176, 1516
TseFI GTSAC 4 cut(s) 530, 1507, 1578, 1627
TseI GCWGC 8 cut(s) 65, 440, 797, 992, 1035, 1161, 1337, 1531
Tsp45I GTSAC 4 cut(s) 530, 1507, 1578, 1627
TspDTI ATGAA 9 cut(s) 162, 315, 636, 657, 1064, 1105, 1244, 1508, 1728
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 4 cut(s) 442, 661, 1176, 1516
Vha464I CTTAAG 1 cut(s) 1615
VneI GTGCAC 2 cut(s) 425, 1636
VpaK11BI GGWCC 1 cut(s) 1174
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 4 cut(s) 150, 263, 356, 1066
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 1648
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 9 cut(s) 48, 131, 651, 822, 996, 1271, 1454, 1632, 1703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.