Rorug06G0337800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001092
Physical Location & Seq
Forward (+)
48977148 .. 48984076
6929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0337800.1

Sequence Viewer

Length: 2658 bp
ATGAGTTCGAAACCATGGCTATACCCGGCTCCTACCTATCGTACTCTCGAGACCTATTGGGATACCGACGACGACGCGCCCGGCGCGCGATGCGGTCACACTCTCACCGCCGTCGCAGCCACCAAGACCCACGGCCCCCGCCTTATTCTCTTCGGTGGCGCCACCGCCATTGAAGGCGGCGCAGCCTCCTCCGCCCCCGGCATCAGGCTAGCGGGTGTGACCAATTCTGTTCACTCCTACGATGTTCTCACCAGAAAGTGGACTAGAATCAGGCCGGCTGGTGAGCCACCCTCTCCGCGGGCGGCGCACGCTGCAGCTGCAGTGGGGACTATGGTGGTTTTTCAGGGTGGCATTGGTCCTGCTGGGCACTCCACGGATGATCTCTATGTGCTTGACATGGATAAGTTCAAGTGGCACAGAGTGGTTGTGCAAGGACAAGGACCTGGGCCTCGCTATGGCCATGTTATGGACTTGGTTGCTCAAAGATATCTTGTTACTGTCAGCGGCAATGATGGAAAAAGAGTTCTTGCTGATTCCTGGGCTCTGGATACTGCACAGAAACCTTACGTATGGGCTAAGCTGAGTCCAGAAGGGGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGCATGTTTTTGCTTTGTGGCGGAAGAGACGCTTCCGGGGCGCCTCTAGCTGATGCTTATGGACTGCTCATGCATAGAAATGGTCAGTGGGAGTGGACTCTCGCACCGGGAGTGTCTCCTTCACCAAGATATCAACATGCTGCGGTTTTTGTTGGTGCAAGATTACATGTAACGGGAGGTGTTCTCAGGGGAGGACGTGCTGTAGAAGGGGAAGGAGCTATTGCAGTATTGGACACTGCCGCTGGAGTTTGGTTAGATAGAAGCGGGCTTGTGACCTCCTCACGGACAGGCAAGGGACAAAATGACTATGATCCCCCTTTGGAGCTTATGCGTCGTTGTCGCCATGCAGCCGCATCTCTTGGTGTTCGTATTTACATTTATGGCGGTCTCAAGGGAGACATCCTGTTAGATGATTTCCTGGTTGCGGAAAATTCATCATTTCAATCTGAAATTAATTCTCCCGTATCAACTTCTGAGAAATCCCCATCTTTAACAAGTCCGAGAGCCTTTCATGCCAACACAAGTTCCTTTGGTGTATCACCATCTTCGGATGGTGAATCTGAGATACCCTCATCTAGTGGCTTGAGCATGGATAAAAATTCCATGGAGAAACTACGGCAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAGTCCCAATCTTCCCCTCCTCCTGAAGAAACTTCTGTTTCAGATGACAACTCACAAATTGCAGAAACACTTTCGGATGGTAGTGACACTGAGGCAGATGTTCGCCTTCATCCCAGAGCTGTTGTTGTTGCCAAAGAGGCTGTAGGCAATTTGGGTGGCATGGTAAGACAGTTGTCTTTGGATCAATTCGAAAATGAGAGCAGACGAATGATTCCAATGAATAACGACTTATCATATCCTAACAAAAAGTTTACCAGGCAGAAGTCCCCACAGGGCTTACATAAGAAGATAATATCTACATTGCTGAGGCCAAGGAACTGGAAACCTCCGGTTAATAGGAAGTTTTTCCTCGATTCTTACGAAGTGGGAGAACTTTGTTATGCTGCTGAACAGATCTTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCTCCTATTAAAGTATTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTGTTTGATGAATATGGATTTCCATCTGCTGCAGGAGACATAACGTACATTGACTACTTGTTTTTGGGGGATTATGTTGATCGAGGACAGCACAGCTTGGAGACAATAACTCTACTCCTTGCACTCAAGATTGAGTATCCTGATAATGTTCACTTAATACGTGGAAACCATGAGGCTGCTGATATCAACGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGAGAGAGTGATGGAATATGGGCATGGACAAGGTTCAATCAACTTTTCAACTACCTCCCACTGGCTGCACTAATTGAAAAGAAAATCATCTGTATGCATGGTGGCATTGGAAGATCTATAAATTCTGTAGAACAAATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGGTCTATAATTTTAATGGATCTTCTATGGTCTGATCCTACAGAAAACGATGGTGTAGAGGGTCTCAGACCAAATGCAAGAGGGCCTGGTCTTGTCACTTTTGGGCCTGATCGTGTCACGGACTTCTGTAAGAGGAACAAATTACAGCTCATTATAAGAGCTCATGAATGTGTTATGGATGGTTTTGAACGGTTTGCCCAGGGACAATTGATCACTCTTTTTTCTGCCACCAACTATTGTGGAACGGCAAACAATGCTGGTGCAATACTGGTGGTTGGCAGGGGGCTGGTTGTAGTTCCAAAACTAATTCATCCGCTGCCACCTCCTCTTCAGTCACCGGAGACATCTCCTGAACGCGTCATAGATGACACATGGATGCAGGAGCTAAACATTCAGAGGCCGCCAACTCCTACTCGTGGTCGACCACAGCCTGATCTTGATCGGAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

885

Amino Acids

96.56

Weight (kDa)

6.0

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 218 2.5e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 22 - 186 1.5e-24 FBX42, beta-propeller domain
Kelch_HCF PF13854 72 - 346 1.2e-15 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 173 - 355 3.5e-12 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 201 - 277 7.6e-07 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 582 - 789 1.6e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2348
AccB1I GGYRCC 2 cut(s) 158, 682
AccB7I CCANNNNNTGG 2 cut(s) 258, 466
AccBSI CCGCTC 1 cut(s) 636
AccI GTMKAC 1 cut(s) 2614
AccII CGCG 5 cut(s) 77, 86, 88, 298, 2550
AclWI GGATC 4 cut(s) 947, 1500, 2220, 2222
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 3 cut(s) 1072, 1240, 2143
AcuI CTGAAG 3 cut(s) 1356, 1715, 2507
AcyI GRCGYC 2 cut(s) 159, 683
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 2 cut(s) 43, 1841
AfiI CCNNNNNNNGG 9 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083, 2609
AflIII ACRYGT 2 cut(s) 808, 2548
AgsI TTSAA 9 cut(s) 173, 409, 1085, 2006, 2059, 2071, 2099, 2171, 2381
AjiI CACGTC 1 cut(s) 839
AjnI CCWGG 6 cut(s) 442, 536, 1059, 1565, 2278, 2391
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
Alw21I GWGCWC 1 cut(s) 2356
Alw26I GTCTC 9 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261, 2528
AlwI GGATC 4 cut(s) 947, 1500, 2220, 2222
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 9 cut(s) 133, 272, 446, 457, 599, 1619, 2276, 2297, 2591
ApoI RAATTY 3 cut(s) 1072, 1240, 2143
AseI ATTAAT 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 1655
AspLEI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
AspS9I GGNCC 6 cut(s) 134, 356, 440, 446, 2276, 2297
AsuC2I CCSGG 5 cut(s) 26, 81, 198, 679, 750
AsuHPI GGTGA 8 cut(s) 97, 241, 293, 756, 1173, 1208, 1793, 2520
AsuII TTCGAA 2 cut(s) 8, 1500
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 2 cut(s) 356, 440
BaeGI GKGCMC 2 cut(s) 369, 634
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 2 cut(s) 158, 682
BanII GRGCYC 2 cut(s) 544, 2356
BauI CACGAG 1 cut(s) 2607
Bbv12I GWGCWC 1 cut(s) 2356
BbvCI CCTCAGC 2 cut(s) 1281, 1616
BceAI ACGGC 4 cut(s) 95, 148, 1274, 2454
BciT130I CCWGG 6 cut(s) 444, 538, 1061, 1567, 2280, 2393
BciVI GTATCC 3 cut(s) 55, 541, 1941
BclI TGATCA 1 cut(s) 2403
BcnI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BcoDI GTCTC 9 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261, 2528
BfaI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
BfmI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2148, 2232
BfoI RGCGCY 2 cut(s) 162, 686
BfuI GTATCC 3 cut(s) 55, 541, 1941
BglI GCCNNNNNGGC 1 cut(s) 1448
BglII AGATCT 2 cut(s) 1704, 2135
BlpI GCTNAGC 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 356, 440
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 6 cut(s) 134, 356, 440, 446, 2276, 2297
BmiI GGNNCC 4 cut(s) 30, 136, 160, 684
BmsI GCATC 6 cut(s) 80, 210, 685, 1004, 2179, 2559
BmtI GCTAGC 1 cut(s) 212
BoxI GACNNNNGTC 1 cut(s) 1483
BplI GAGNNNNNCTC 6 cut(s) 275, 307, 810, 842, 1196, 1228
BpmI CTGGAG 1 cut(s) 906
Bpu10I CCTNAGC 2 cut(s) 1281, 1616
Bpu1102I GCTNAGC 1 cut(s) 576
Bpu14I TTCGAA 2 cut(s) 8, 1500
BpuEI CTTGAG 3 cut(s) 1016, 1246, 1904
BpuMI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BsaAI YACGTR 2 cut(s) 568, 1955
BsaBI GATNNNNATC 2 cut(s) 1816, 2631
BsaHI GRCGYC 2 cut(s) 159, 683
BsaI GGTCTC 3 cut(s) 44, 1034, 2261
BsaWI WCCGGW 2 cut(s) 1639, 2530
Bsc4I CCNNNNNNNGG 9 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083, 2609
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 4 cut(s) 627, 1634, 2088, 2466
Bse3DI GCAATG 2 cut(s) 514, 1610
Bse8I GATNNNNATC 2 cut(s) 1816, 2631
BseBI CCWGG 6 cut(s) 444, 538, 1061, 1567, 2280, 2393
BseGI GGATG 9 cut(s) 382, 1041, 1198, 1393, 1420, 2194, 2377, 2503, 2574
BseJI GATNNNNATC 2 cut(s) 1816, 2631
BseLI CCNNNNNNNGG 9 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083, 2609
BseMI GCAATG 2 cut(s) 514, 1610
BseMII CTCAG 9 cut(s) 572, 651, 841, 1107, 1194, 1272, 1392, 1607, 2272
BseNI ACTGG 4 cut(s) 627, 1634, 2088, 2466
BsePI GCGCGC 1 cut(s) 84
BseRI GAGGAG 4 cut(s) 178, 910, 1320, 2508
BseSI GKGCMC 2 cut(s) 369, 634
BseYI CCCAGC 2 cut(s) 362, 2192
BsgI GTGCAG 3 cut(s) 537, 1331, 2073
Bsh1236I CGCG 5 cut(s) 77, 86, 88, 298, 2550
BshFI GGCC 9 cut(s) 135, 274, 448, 459, 601, 1621, 2278, 2299, 2593
BshNI GGYRCC 2 cut(s) 158, 682
BsiHKAI GWGCWC 1 cut(s) 2356
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2531
BslFI GGGAC 5 cut(s) 340, 951, 1300, 1561, 2409
BslI CCNNNNNNNGG 9 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083, 2609
BsmAI GTCTC 9 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261, 2528
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 5 cut(s) 340, 951, 1300, 1561, 2409
BsmI GAATGC 1 cut(s) 2012
BsnI GGCC 9 cut(s) 135, 274, 448, 459, 601, 1621, 2278, 2299, 2593
Bso31I GGTCTC 3 cut(s) 44, 1034, 2261
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 2 cut(s) 8, 1500
Bsp1286I GDGCHC 4 cut(s) 369, 544, 634, 2356
Bsp1720I GCTNAGC 1 cut(s) 576
Bsp19I CCATGG 2 cut(s) 14, 1245
BspANI GGCC 9 cut(s) 135, 274, 448, 459, 601, 1621, 2278, 2299, 2593
BspCNI CTCAG 9 cut(s) 573, 650, 840, 1108, 1195, 1273, 1393, 1608, 2271
BspFNI CGCG 5 cut(s) 77, 86, 88, 298, 2550
BspHI TCATGA 1 cut(s) 2356
BspLI GGNNCC 4 cut(s) 30, 136, 160, 684
BspMAI CTGCAG 3 cut(s) 316, 322, 1828
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 4 cut(s) 947, 1500, 2220, 2222
BspT104I TTCGAA 2 cut(s) 8, 1500
BspT107I GGYRCC 2 cut(s) 158, 682
BspTNI GGTCTC 3 cut(s) 44, 1034, 2261
BsrBI CCGCTC 1 cut(s) 636
BsrDI GCAATG 2 cut(s) 514, 1610
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 4 cut(s) 627, 1634, 2088, 2466
BssAI RCCGGY 1 cut(s) 274
BssHII GCGCGC 1 cut(s) 84
BssNI GRCGYC 2 cut(s) 159, 683
BssSI CACGAG 1 cut(s) 2607
BssT1I CCWWGG 3 cut(s) 14, 1245, 1622
Bst2BI CACGAG 1 cut(s) 2607
Bst2UI CCWGG 6 cut(s) 444, 538, 1061, 1567, 2280, 2393
Bst4CI ACNGT 5 cut(s) 499, 1482, 1726, 1776, 2385
Bst6I CTCTTC 3 cut(s) 155, 661, 2526
BstACI GRCGYC 2 cut(s) 159, 683
BstAPI GCANNNNNTGC 3 cut(s) 1292, 1309, 2447
BstBAI YACGTR 2 cut(s) 568, 1955
BstBI TTCGAA 2 cut(s) 8, 1500
BstC8I GCNNGC 9 cut(s) 86, 210, 276, 300, 309, 634, 908, 1263, 1305
BstDSI CCRYGG 5 cut(s) 14, 130, 296, 372, 1245
BstF5I GGATG 9 cut(s) 382, 1041, 1198, 1393, 1420, 2194, 2377, 2503, 2574
BstFNI CGCG 5 cut(s) 77, 86, 88, 298, 2550
BstH2I RGCGCY 2 cut(s) 162, 686
BstHHI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
BstMAI GTCTC 9 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261, 2528
BstNI CCWGG 6 cut(s) 444, 538, 1061, 1567, 2280, 2393
BstNSI RCATGY 3 cut(s) 649, 782, 812
BstPAI GACNNNNGTC 1 cut(s) 1483
BstSFI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2148, 2232
BstSLI GKGCMC 2 cut(s) 369, 634
BstSNI TACGTA 1 cut(s) 568
BstUI CGCG 5 cut(s) 77, 86, 88, 298, 2550
BstX2I RGATCY 3 cut(s) 1704, 2135, 2212
BstXI CCANNNNNNTGG 2 cut(s) 1629, 2185
BstYI RGATCY 3 cut(s) 1704, 2135, 2212
BsuI GTATCC 3 cut(s) 55, 541, 1941
BsuRI GGCC 9 cut(s) 135, 274, 448, 459, 601, 1621, 2278, 2299, 2593
BtgI CCRYGG 5 cut(s) 14, 130, 296, 372, 1245
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 9 cut(s) 382, 1041, 1198, 1393, 1420, 2194, 2377, 2503, 2574
BtsI GCAGTG 2 cut(s) 327, 876
BtsIMutI CAGTG 6 cut(s) 327, 634, 734, 876, 1398, 2081
Cac8I GCNNGC 9 cut(s) 86, 210, 276, 300, 309, 634, 908, 1263, 1305
CciI TCATGA 1 cut(s) 2356
CfoI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 6 cut(s) 134, 356, 440, 446, 2276, 2297
Cfr42I CCGCGG 1 cut(s) 299
CseI GACGC 4 cut(s) 83, 680, 962, 2539
Csp6I GTAC 2 cut(s) 42, 1840
CspCI CAANNNNNGTGG 8 cut(s) 151, 186, 1447, 1482, 2413, 2445, 2448, 2480
CviQI GTAC 2 cut(s) 42, 1840
DinI GGCGCC 2 cut(s) 160, 684
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 3 cut(s) 155, 661, 2526
EarI CTCTTC 3 cut(s) 155, 661, 2526
EciI GGCGGA 2 cut(s) 181, 678
Ecl136II GAGCTC 1 cut(s) 2354
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 3 cut(s) 14, 1245, 1622
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 2 cut(s) 544, 2356
Eco31I GGTCTC 3 cut(s) 44, 1034, 2261
Eco32I GATATC 3 cut(s) 488, 773, 1978
Eco47I GGWCC 2 cut(s) 356, 440
Eco53kI GAGCTC 1 cut(s) 2354
Eco57I CTGAAG 3 cut(s) 1356, 1715, 2507
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 2354
EcoO109I RGGNCCY 2 cut(s) 440, 2276
EcoRII CCWGG 6 cut(s) 442, 536, 1059, 1565, 2278, 2391
EcoRV GATATC 3 cut(s) 488, 773, 1978
EcoT14I CCWWGG 3 cut(s) 14, 1245, 1622
EcoT22I ATGCAT 3 cut(s) 717, 2012, 2121
EcoT38I GRGCYC 2 cut(s) 544, 2356
EgeI GGCGCC 2 cut(s) 160, 684
EheI GGCGCC 2 cut(s) 160, 684
ErhI CCWWGG 3 cut(s) 14, 1245, 1622
Esp3I CGTCTC 1 cut(s) 663
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 5 cut(s) 340, 951, 1300, 1561, 2409
FauI CCCGC 5 cut(s) 146, 205, 291, 641, 899
FbaI TGATCA 1 cut(s) 2403
FblI GTMKAC 1 cut(s) 2614
FokI GGATG 9 cut(s) 389, 1028, 1205, 1400, 1407, 2201, 2384, 2490, 2581
FriOI GRGCYC 2 cut(s) 544, 2356
FspBI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
GlaI GCGC 8 cut(s) 78, 85, 87, 160, 181, 306, 684, 1793
GsaI CCCAGC 2 cut(s) 366, 2196
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 2 cut(s) 162, 686
HaeIII GGCC 9 cut(s) 135, 274, 448, 459, 601, 1621, 2278, 2299, 2593
HapII CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2531
HgaI GACGC 4 cut(s) 83, 680, 962, 2539
HhaI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
Hin1I GRCGYC 2 cut(s) 159, 683
Hin6I GCGC 8 cut(s) 77, 84, 86, 159, 180, 305, 683, 1792
HinP1I GCGC 8 cut(s) 77, 84, 86, 159, 180, 305, 683, 1792
HincII GTYRAC 1 cut(s) 2615
HindII GTYRAC 1 cut(s) 2615
HindIII AAGCTT 1 cut(s) 2165
HinfI GANTC 7 cut(s) 267, 533, 583, 739, 1199, 1522, 1664
HpaII CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2531
HphI GGTGA 8 cut(s) 97, 241, 293, 756, 1173, 1208, 1793, 2520
Hpy166II GTNNAC 6 cut(s) 232, 261, 738, 1563, 1945, 2615
Hpy8I GTNNAC 6 cut(s) 232, 261, 738, 1563, 1945, 2615
Hpy99I CGWCG 5 cut(s) 71, 74, 77, 116, 978
HpyAV CCTTC 7 cut(s) 167, 584, 612, 771, 842, 848, 1427
HpyCH4III ACNGT 5 cut(s) 499, 1482, 1726, 1776, 2385
HpyCH4IV ACGT 4 cut(s) 567, 838, 1838, 1954
HpySE526I ACGT 4 cut(s) 567, 838, 1838, 1954
Hsp92I GRCGYC 2 cut(s) 159, 683
HspAI GCGC 8 cut(s) 77, 84, 86, 159, 180, 305, 683, 1792
KasI GGCGCC 2 cut(s) 158, 682
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 2403
KspI CCGCGG 1 cut(s) 299
LmnI GCTCC 6 cut(s) 34, 857, 964, 1717, 1747, 2575
LweI GCATC 6 cut(s) 80, 210, 685, 1004, 2179, 2559
MaeI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
MaeII ACGT 4 cut(s) 567, 838, 1838, 1954
MaeIII GTNAC 9 cut(s) 95, 217, 493, 811, 913, 1394, 2287, 2308, 2526
MbiI CCGCTC 1 cut(s) 636
MfeI CAATTG 1 cut(s) 2399
MflI RGATCY 3 cut(s) 1704, 2135, 2212
MhlI GDGCHC 4 cut(s) 369, 544, 634, 2356
MlsI TGGCCA 1 cut(s) 459
MluI ACGCGT 1 cut(s) 2548
MluNI TGGCCA 1 cut(s) 459
Mly113I GGCGCC 2 cut(s) 159, 683
MlyI GAGTC 2 cut(s) 592, 733
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 3 cut(s) 717, 2012, 2121
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 1655
MscI TGGCCA 1 cut(s) 459
MseI TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
MslI CAYNNNNRTG 5 cut(s) 720, 2114, 2183, 2361, 2567
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 6 cut(s) 298, 317, 504, 884, 1735, 2509
MspI CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2531
MunI CAATTG 1 cut(s) 2399
Mva1269I GAATGC 1 cut(s) 2012
MvaI CCWGG 6 cut(s) 444, 538, 1061, 1567, 2280, 2393
MvnI CGCG 5 cut(s) 77, 86, 88, 298, 2550
NaeI GCCGGC 1 cut(s) 276
NarI GGCGCC 2 cut(s) 159, 683
NciI CCSGG 5 cut(s) 26, 81, 198, 679, 750
NcoI CCATGG 2 cut(s) 14, 1245
NgoMIV GCCGGC 1 cut(s) 274
NheI GCTAGC 1 cut(s) 208
NlaIV GGNNCC 4 cut(s) 30, 136, 160, 684
NmuCI GTSAC 7 cut(s) 95, 217, 913, 1394, 2287, 2308, 2526
NsiI ATGCAT 3 cut(s) 717, 2012, 2121
NspI RCATGY 3 cut(s) 649, 782, 812
NspV TTCGAA 2 cut(s) 8, 1500
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2356
PasI CCCWGGG 1 cut(s) 2392
PauI GCGCGC 1 cut(s) 84
PceI AGGCCT 1 cut(s) 601
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 1 cut(s) 1668
PctI GAATGC 1 cut(s) 2012
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1655
PfeI GAWTC 5 cut(s) 267, 533, 1199, 1522, 1664
PflMI CCANNNNNTGG 2 cut(s) 258, 466
PleI GAGTC 2 cut(s) 591, 733
PluTI GGCGCC 2 cut(s) 162, 686
PpsI GAGTC 2 cut(s) 591, 733
Ppu21I YACGTR 2 cut(s) 568, 1955
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 808
PshAI GACNNNNGTC 1 cut(s) 1483
PshBI ATTAAT 1 cut(s) 1095
PsiI TTATAA 1 cut(s) 2348
Psp124BI GAGCTC 1 cut(s) 2356
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 6 cut(s) 442, 536, 1059, 1565, 2278, 2391
PspFI CCCAGC 2 cut(s) 362, 2192
PspGI CCWGG 6 cut(s) 442, 536, 1059, 1565, 2278, 2391
PspN4I GGNNCC 4 cut(s) 30, 136, 160, 684
PspPI GGNCC 6 cut(s) 134, 356, 440, 446, 2276, 2297
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 3 cut(s) 316, 322, 1828
PsuI RGATCY 3 cut(s) 1704, 2135, 2212
PteI GCGCGC 1 cut(s) 84
PvuII CAGCTG 2 cut(s) 317, 1735
RsaI GTAC 2 cut(s) 43, 1841
RsaNI GTAC 2 cut(s) 42, 1840
RseI CAYNNNNRTG 5 cut(s) 720, 2114, 2183, 2361, 2567
SacI GAGCTC 1 cut(s) 2356
SacII CCGCGG 1 cut(s) 299
SalI GTCGAC 1 cut(s) 2613
SaqAI TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
Sau96I GGNCC 6 cut(s) 134, 356, 440, 446, 2276, 2297
SchI GAGTC 2 cut(s) 592, 733
SduI GDGCHC 4 cut(s) 369, 544, 634, 2356
SfaNI GCATC 6 cut(s) 80, 210, 685, 1004, 2179, 2559
SfcI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2148, 2232
SfoI GGCGCC 2 cut(s) 160, 684
Sfr274I CTCGAG 1 cut(s) 47
Sfr303I CCGCGG 1 cut(s) 299
SfuI TTCGAA 2 cut(s) 8, 1500
SgrBI CCGCGG 1 cut(s) 299
SinI GGWCC 2 cut(s) 356, 440
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 5 cut(s) 720, 2114, 2183, 2361, 2567
SmlI CTYRAG 4 cut(s) 47, 1031, 1225, 1919
SmoI CTYRAG 4 cut(s) 47, 1031, 1225, 1919
SnaBI TACGTA 1 cut(s) 568
SseBI AGGCCT 1 cut(s) 601
SspDI GGCGCC 2 cut(s) 158, 682
SspMI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
SstI GAGCTC 1 cut(s) 2356
StuI AGGCCT 1 cut(s) 601
StyI CCWWGG 3 cut(s) 14, 1245, 1622
TaaI ACNGT 5 cut(s) 499, 1482, 1726, 1776, 2385
TaiI ACGT 4 cut(s) 570, 841, 1841, 1957
TaqI TCGA 6 cut(s) 8, 48, 1500, 1662, 1876, 2614
TauI GCSGC 6 cut(s) 180, 305, 507, 884, 995, 2596
TfiI GAWTC 5 cut(s) 267, 533, 1199, 1522, 1664
Tru1I TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
Tru9I TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
TscAI CASTG 6 cut(s) 327, 634, 734, 883, 1405, 2088
TseFI GTSAC 7 cut(s) 95, 217, 913, 1394, 2287, 2308, 2526
Tsp45I GTSAC 7 cut(s) 95, 217, 913, 1394, 2287, 2308, 2526
TspDTI ATGAA 8 cut(s) 1065, 1142, 1409, 1544, 1757, 1818, 2373, 2492
TspGWI ACGGA 3 cut(s) 389, 940, 2327
TspRI CASTG 6 cut(s) 327, 634, 734, 883, 1405, 2088
Van91I CCANNNNNTGG 2 cut(s) 258, 466
VpaK11BI GGWCC 2 cut(s) 356, 440
VspI ATTAAT 1 cut(s) 1095
XapI RAATTY 3 cut(s) 1072, 1240, 2143
XceI RCATGY 3 cut(s) 649, 782, 812
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 2614
XmnI GAANNNNTTC 1 cut(s) 1655
XspI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
Zsp2I ATGCAT 3 cut(s) 717, 2012, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.