pycom15g17120

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001838
Physical Location & Seq
Reverse (-)
12012110 .. 12019086
6977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4038 bp
ATGACCACCGCCACCATGACCTCCGCCTCATCCTCCCAAATCCTCGGCCACACTGCCACCTTCATCTCCAAAACCTTATCCCAACCCGAGCTCCGCCGCCACCTCTTCTCCACTCTCCGCCGTAAACTCTCTCCCTCCACCTCCCAAGCCACCCTCAAACCCTTAAACCTGGCCGCGGAAACCCTGGAAAACGCAATCTCCACCACCAGCCCCGCCATTTGTGCCTCCTCCCTCACCCTTTCCGAAAAGCTCCTCCGGTCAACCCCCAAAAACCTCTTCTCTTCCCTCCTCCTCTCCCTCATCTACGGTCTCTCCAGACGCCCCATTGACGCTGCTCTCAGCCTCCTAGACATCTTCCACACCGACCCTTCATTCGCCCGAACGGAACTCGCCCCCATACTCTTTGAAGAGCTCTTTCTAGTCCACCTCCTCCCTGTCGTCCATTGGTACAACGAACAAAGATCACAAATATTACCAACATTGTCCCCGAACATTAGCTATGAAAATGACGACTGTTCTATATCCGACATGTCCGTCGTGTTTTCGTGCTCGAAATCGTTGTCTAAGATGAGTGGGGGGCAGACTTCGGAGCTAAAGGAGTTGGAATCTAATTACGAGAAAGTTCTTGATGAAAATTGCAGGGTTTTTAGTAATTATTTCAAACAAGTTATTGAAAATAAAGATGCGAATAGACGGCTTGATCTGCCCGCAGTGATTTTGAAGAGAGTTGTTGGACTTGGCGAAGTCAAGAATGGAGATGATGATGATGAACGAGGGATCAAAATGGAGGAACCCGGATTCCAAAACGGACGGTATAATCCAATATGGGCTGAAGCAGAAAGACCAGTTGAAGTACTCAGCAGCAGAAGAAAACCAAAGTCTCCACCTTTTTATCCTCAAAGAGTTTCAATCAGCAATATCCTCAAAACCCAGGAACAGGAACCTTCCTGGAACTTGAAATCTTCGCCCAATGTGAATTCTTCAGATTCCGAGTCAGAGTCTTTCCTGGAGGATATTTCGGTAAACTCCTCTTCTTCCTTGGATTACGGAGCAGAAATTGAGGAGGAAAACAACAGAGAAATGGAATTGTTTGAGGCTACACAGAGCCAAATAAAGAAGCTAAAGCAACCCATTTCTGCAGAATCGAGCCGTTCTCCAGACCAGCTGATCATGGCAGATTGTGATAACAATTCTGTTGGGGGAAAAGTACATACACCTCCCAAGGATTTTGTGTGTCCCATTACTAGCACTTTGTTTGATGATCCTGTGACACTTGAGACGGGACAGACATATGAGCGAAAAGCGATCCAGGAGTGGATCGAAAGAGGGAACTCTACGTGCCCAATTACGCGCCAGAAATTGCAGAGCACTCAACTGCCTAAGACCAACTATGTGCTCAAAAGGCTTATTGCAAGCTGGCAAGAACAGAATCCTAGTGCAGTGTTGAGCCAATCGGAGAGTACAAGCCCATTGATTGAGCCTGTTATGAAGGCCATAATGCCTTCAACTTCTCCCGACAGTGTTATAACCCAAGGTGGGTTTGATGGCGCAGTTGGCGAGTTAAGACTCGCAATTACCAACCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCAAATTGAGCGGTTCTGGCAGGAGGCAAATGTGGAATGGGATATTCAGAATTTGCTCACGAGACCTCCAGTGATCAACGGTTTTGTGGAGGTTCTTTTCAATTCTGTTGATCCCAGGGTTCTGTTAGCTGCAGTTTTTCTCCTGTCTGAGCTGGGGTCAAGAGACAATGCTGTGATCCAGACGCTAACGCGCGTTGATTCTGATGTTGAATGTATTGTGGCGCTTTTTAAAAAGGGGTTGATGGAGGCTGTGGTGTTGATATATCTGTTGAGGCATTCGATCCTGAACCTAATTGAGATGGGAATGGTGGAGTCTCTCTTACTGGTTATCAAAAGGAAAGACGATGATTTGCTTAACATGTGTCTAAAGCCGAGAACAGCTGCAGTGGTTTTACTTGGGCTGATTCTTGGTGGCAGTGAAGAGGGTATTGCCTCTTCGATTGTTAACACAGTGGTTTCTGAAAAAGCGCTTGAAACAATTATTAGAAGTTTGGAATCTGAATCGACCGAAGAGAGGATTGCAGCAGTTAGGATCATGTTGAGATGTATGCAGCAGGATGGAAAGTGCAGGAACACAATAGCCGATAAAGCTGAGCTGGCTCCTGTTTTGGATAGCTTTGTCAGCGTAAGTGATGGAGAAAAGTTTGAGATAGTTCACTTTTTCTCCGAGTTAGTTAAATTAAACAGAAGGACATTTAATGAACAAATCCTGCATATCATAAAGGATGAAGGCTCTCTCAGCACAATGCACACCCTTCTTATTTATCTGCAGACTGCCCCTCCAGTTCAGTGTCCTATTGTGGCCGGTCTACTACTTCAACTCGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGCGAAGAGGCAATAGACACGCTCATCTCATGTCTCAGAAATGCGGACTTCCCTACTGCCCAAATTGCTGCTGCTGAGACAATTATGTCGCTGCAAGGGAGGTTCACCACATCCGGAAAGCCTCTCACCAGGGCTTTCCTTCTCAAGCGTGCTGGCCTTGACAAAAGTTACAAAAATCATGTGCGAATGGATCAACTTAGCAATTTTTCTGGAGATGACGAAACTCTTGAGGAAGAGAAGGCGGCTGATAATTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCATGAGTTTGGTCTACTTTTTGAGGCTTTAGCCGAAGGCTTGAAGAGCAGATATGCGGAATTATGTTCAGCGTGCTTTGTGATAGCCACATGGCTCGTACACATGCTCAATGTTCTTCCAGACACAGGAATACGTGGAGCAGCCCGCGTCTGCTTGCTCAAACGTTACATGTCGATATTCAAGTCTGCCAAGGACACCGATGACAAAGCCCTCTCTATGCTTGCGTTGACCAGCTTCAGCCAAGATCCTGAGGGGATGCAAGAGGTAACTTCTTCCATAAAGGATATCGTAAAAGGCCTGAGAGAGCTTAAGAGATCGACTCCCTTGGCATTTCAATTGCTAAAACTATTTTCTGAGGGGCAAGACTCTAGCGCCGAACTGTGGGATCATAAAGAATTAGTTCAAGTAGATTGCAGCGAGAATGGAGAGGTTCTTTCAGTCATTTGCTTCAAGGACAAAATATTTTCGGGCCACTCGGATGGAACTATAAAGGTATGGACGGGTAAAGGTAGCGTTCTTCATCTAATCCAAGAAACTCGAGAACACACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCGGGGGAAGTTTTATACAGTGGCTCGCTCGATAGAACTACAAGGGTATGGTCTATTAGCAATGAAGCAATGTACTGTGTACAAGTACACGACATGAAGGATCAAATTCATAGTTTAGCCGTCACGAATAGCCTCGCTTGCTTCATTCCACAAAGTACCGGTGTCAAGGTTCATTCGTGGAATGGAGGAACCAAATTGTTAAATTCAAGTAAACATGTGAGGTGCTTCGCTCTACTACATGGAAAATTATATTGTGGATGCAATGACAATGGTATTCAGGAGATTGATTTGGCCACGGGAACACTCAGTACCATTCAAAATGGCACTCGGAAACTACTCACGAAATCAAACCCTATCCACGCCATTCAAGTGCACAGTGGACTTATCTATACAGCCAGTTCTTCCGCGGATGGGGCAGCTGTTAAGATATGGAATGCTGCAAACTCCAACCTGGTTGGATCACTTCCGACCACATTGGAAGTGCGGGCGATGGCCATAAGCTCGGAACTGATTTATTTGGGAGGTAAAGGAGGAAGCGTGGAAATATGGGATAGGAATAAGCAGAACAGAATAGACACCCTACAAACTGGTACCAATTGCAAGGTCCTTTGCTTGGCTCTTGATGCCAATGAGGACGTTTTTGTCACTGGAACTTCTGATGGCCGAATTCGGGCATGGGGACTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1346

Amino Acids

148.86

Weight (kDa)

5.51

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 16 - 232 1.6e-21 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 407 - 477 4.4e-17 U-box domain
ARM_LIN_2nd PF23654 517 - 674 3e-59 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 677 - 1041 8.8e-149 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 1040 - 1154 1.7e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1040 - 1179 1e-08 WDR5 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 1043 - 1145 9.8e-06 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 1045 - 1151 5.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1050 - 1180 1.3e-10 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 1066 - 1159 1.1e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 1074 - 1159 8.9e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 1074 - 1261 4.6e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1075 - 1139 1.4e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 1075 - 1151 3.4e-07 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1189 - 1343 2.3e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1231 - 1343 3.2e-07 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1246 - 1344 4.7e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 1248 - 1344 3e-06 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 1277 - 1343 3.9e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1526
AasI GACNNNNNNGTC 3 cut(s) 533, 2569, 3989
Acc65I GGTACC 1 cut(s) 3938
AccB1I GGYRCC 1 cut(s) 3938
AccBSI CCGCTC 1 cut(s) 1636
AccI GTMKAC 2 cut(s) 2434, 2782
AccII CGCG 7 cut(s) 176, 1351, 1816, 1818, 2487, 2916, 3755
AccIII TCCGGA 1 cut(s) 2597
AclI AACGTT 1 cut(s) 2932
AcoI YGGCCR 6 cut(s) 46, 171, 2427, 3639, 3840, 4009
AcsI RAATTY 6 cut(s) 976, 1596, 1675, 3453, 3550, 4014
AcuI CTGAAG 3 cut(s) 852, 966, 2989
AcyI GRCGYC 1 cut(s) 319
AfeI AGCGCT 1 cut(s) 2094
AflII CTTAAG 1 cut(s) 3077
AflIII ACRYGT 4 cut(s) 528, 1983, 2937, 3562
AgeI ACCGGT 1 cut(s) 3506
AjnI CCWGG 9 cut(s) 168, 183, 930, 947, 1005, 1308, 1739, 2612, 3798
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AloI GAACNNNNNNTCC 2 cut(s) 783, 815
Alw21I GWGCWC 6 cut(s) 93, 414, 551, 1370, 1398, 3723
Alw26I GTCTC 8 cut(s) 314, 885, 1271, 1681, 1782, 1944, 2522, 2555
Alw44I GTGCAC 1 cut(s) 3719
Ama87I CYCGRG 2 cut(s) 86, 3306
Aor13HI TCCGGA 1 cut(s) 2597
Aor51HI AGCGCT 1 cut(s) 2094
ApaLI GTGCAC 1 cut(s) 3719
ApoI RAATTY 6 cut(s) 976, 1596, 1675, 3453, 3550, 4014
ArsI GACNNNNNNTTYG 4 cut(s) 356, 388, 545, 577
AsiGI ACCGGT 1 cut(s) 3506
Asp700I GAANNNNTTC 1 cut(s) 3168
Asp718I GGTACC 1 cut(s) 3938
AspLEI GCGC 6 cut(s) 1353, 1550, 1818, 1849, 2095, 3143
AspS9I GGNCC 2 cut(s) 3238, 3952
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 3 cut(s) 226, 2581, 2602
AvaI CYCGRG 2 cut(s) 86, 3306
AvaII GGWCC 1 cut(s) 3952
AxyI CCTNAGG 1 cut(s) 3018
BaeGI GKGCMC 2 cut(s) 1343, 3723
BalI TGGCCA 2 cut(s) 3641, 3842
BanI GGYRCC 1 cut(s) 3938
BanII GRGCYC 2 cut(s) 93, 414
BarI GAAGNNNNNNTAC 2 cut(s) 2044, 2076
BauI CACGAG 1 cut(s) 1684
Bbv12I GWGCWC 6 cut(s) 93, 414, 551, 1370, 1398, 3723
BccI CCATC 9 cut(s) 1538, 1861, 1918, 2177, 2252, 3242, 3752, 3832, 4001
BceAI ACGGC 4 cut(s) 105, 710, 1134, 3452
BciT130I CCWGG 9 cut(s) 170, 185, 932, 949, 1007, 1310, 1741, 2614, 3800
BclI TGATCA 2 cut(s) 1167, 1698
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 8 cut(s) 314, 885, 1271, 1681, 1782, 1944, 2522, 2555
BfaI CTAG 6 cut(s) 347, 419, 1245, 1434, 3138, 3330
BfmI CTRYAG 4 cut(s) 1137, 1755, 2007, 2393
BfoI RGCGCY 3 cut(s) 1850, 2096, 3144
BfrI CTTAAG 1 cut(s) 3077
BlpI GCTNAGC 1 cut(s) 2217
BmcAI AGTACT 1 cut(s) 855
Bme18I GGWCC 1 cut(s) 3952
BmeT110I CYCGRG 2 cut(s) 86, 3306
BmgT120I GGNCC 2 cut(s) 3238, 3952
BmiI GGNNCC 5 cut(s) 792, 942, 2226, 3538, 3940
BmsI GCATC 4 cut(s) 673, 3015, 3596, 3961
BplI GAGNNNNNCTC 4 cut(s) 1138, 1170, 3073, 3105
BpmI CTGGAG 6 cut(s) 298, 1028, 1140, 1677, 2391, 2715
Bpu1102I GCTNAGC 1 cut(s) 2217
BpuEI CTTGAG 4 cut(s) 1295, 1586, 2612, 2732
BpuMI CCSGG 1 cut(s) 795
BsaAI YACGTR 2 cut(s) 1338, 2903
BsaHI GRCGYC 1 cut(s) 319
BsaI GGTCTC 2 cut(s) 314, 1681
BsaWI WCCGGW 3 cut(s) 255, 2597, 3506
Bse118I RCCGGY 2 cut(s) 2429, 3506
Bse1I ACTGG 7 cut(s) 845, 1694, 1953, 2408, 3744, 3940, 4000
Bse21I CCTNAGG 1 cut(s) 3018
Bse3DI GCAATG 3 cut(s) 3415, 3423, 3616
BseAI TCCGGA 1 cut(s) 2597
BseBI CCWGG 9 cut(s) 170, 185, 932, 949, 1007, 1310, 1741, 2614, 3800
BseGI GGATG 8 cut(s) 29, 2188, 2356, 2594, 3030, 3253, 3611, 3763
BseMI GCAATG 3 cut(s) 3415, 3423, 3616
BseNI ACTGG 7 cut(s) 845, 1694, 1953, 2408, 3744, 3940, 4000
BseRI GAGGAG 7 cut(s) 217, 242, 278, 281, 419, 1018, 1076
BseSI GKGCMC 2 cut(s) 1343, 3723
BseYI CCCAGC 1 cut(s) 1777
BsgI GTGCAG 2 cut(s) 1458, 2212
Bsh1236I CGCG 7 cut(s) 176, 1351, 1816, 1818, 2487, 2916, 3755
Bsh1285I CGRYCG 1 cut(s) 2133
BshNI GGYRCC 1 cut(s) 3938
BshTI ACCGGT 1 cut(s) 3506
BsiEI CGRYCG 1 cut(s) 2133
BsiHKAI GWGCWC 6 cut(s) 93, 414, 551, 1370, 1398, 3723
BsiHKCI CYCGRG 2 cut(s) 86, 3306
BsiSI CCGG 5 cut(s) 256, 795, 2430, 2598, 3507
BslFI GGGAC 3 cut(s) 469, 1221, 1296
BsmAI GTCTC 8 cut(s) 314, 885, 1271, 1681, 1782, 1944, 2522, 2555
BsmBI CGTCTC 1 cut(s) 1271
BsmFI GGGAC 3 cut(s) 469, 1221, 1296
BsmI GAATGC 2 cut(s) 1900, 3787
Bso31I GGTCTC 2 cut(s) 314, 1681
BsoBI CYCGRG 2 cut(s) 86, 3306
Bsp1286I GDGCHC 7 cut(s) 93, 414, 551, 1343, 1370, 1398, 3723
Bsp13I TCCGGA 1 cut(s) 2597
Bsp1407I TGTACA 1 cut(s) 3427
Bsp1720I GCTNAGC 1 cut(s) 2217
BspEI TCCGGA 1 cut(s) 2597
BspFNI CGCG 7 cut(s) 176, 1351, 1816, 1818, 2487, 2916, 3755
BspLI GGNNCC 5 cut(s) 792, 942, 2226, 3538, 3940
BspMAI CTGCAG 4 cut(s) 1141, 1759, 2011, 2397
BspQI GCTCTTC 2 cut(s) 402, 2807
BspT107I GGYRCC 1 cut(s) 3938
BspTI CTTAAG 1 cut(s) 3077
BspTNI GGTCTC 2 cut(s) 314, 1681
BsrBI CCGCTC 1 cut(s) 1636
BsrDI GCAATG 3 cut(s) 3415, 3423, 3616
BsrFI RCCGGY 2 cut(s) 2429, 3506
BsrGI TGTACA 1 cut(s) 3427
BsrI ACTGG 7 cut(s) 845, 1694, 1953, 2408, 3744, 3940, 4000
BssAI RCCGGY 2 cut(s) 2429, 3506
BssNI GRCGYC 1 cut(s) 319
BssSI CACGAG 1 cut(s) 1684
BssT1I CCWWGG 5 cut(s) 1038, 1221, 1531, 2958, 3093
Bst2BI CACGAG 1 cut(s) 1684
Bst2UI CCWGG 9 cut(s) 170, 185, 932, 949, 1007, 1310, 1741, 2614, 3800
BstACI GRCGYC 1 cut(s) 319
BstAFI CTTAAG 1 cut(s) 3077
BstAUI TGTACA 1 cut(s) 3427
BstBAI YACGTR 2 cut(s) 1338, 2903
BstDSI CCRYGG 3 cut(s) 174, 3642, 3753
BstF5I GGATG 8 cut(s) 29, 2188, 2356, 2594, 3030, 3253, 3611, 3763
BstFNI CGCG 7 cut(s) 176, 1351, 1816, 1818, 2487, 2916, 3755
BstH2I RGCGCY 3 cut(s) 1850, 2096, 3144
BstHHI GCGC 6 cut(s) 1353, 1550, 1818, 1849, 2095, 3143
BstMAI GTCTC 8 cut(s) 314, 885, 1271, 1681, 1782, 1944, 2522, 2555
BstMCI CGRYCG 1 cut(s) 2133
BstNI CCWGG 9 cut(s) 170, 185, 932, 949, 1007, 1310, 1741, 2614, 3800
BstNSI RCATGY 5 cut(s) 532, 1987, 2875, 2941, 3566
BstSFI CTRYAG 4 cut(s) 1137, 1755, 2007, 2393
BstSLI GKGCMC 2 cut(s) 1343, 3723
BstUI CGCG 7 cut(s) 176, 1351, 1816, 1818, 2487, 2916, 3755
BstX2I RGATCY 1 cut(s) 3013
BstXI CCANNNNNNTGG 2 cut(s) 2777, 3248
BstYI RGATCY 1 cut(s) 3013
Bsu36I CCTNAGG 1 cut(s) 3018
BtgI CCRYGG 3 cut(s) 174, 3642, 3753
BtgZI GCGATG 1 cut(s) 3851
BtsCI GGATG 8 cut(s) 29, 2188, 2356, 2594, 3030, 3253, 3611, 3763
BtsI GCAGTG 5 cut(s) 51, 717, 1446, 2016, 2047
CfoI GCGC 6 cut(s) 1353, 1550, 1818, 1849, 2095, 3143
Cfr10I RCCGGY 2 cut(s) 2429, 3506
Cfr13I GGNCC 2 cut(s) 3238, 3952
Cfr42I CCGCGG 2 cut(s) 177, 3756
CseI GACGC 4 cut(s) 327, 338, 1816, 2905
CsiI ACCWGGT 1 cut(s) 3798
CspAI ACCGGT 1 cut(s) 3506
DraI TTTAAA 1 cut(s) 1855
DrdI GACNNNNNNGTC 3 cut(s) 533, 2569, 3989
DseDI GACNNNNNNGTC 3 cut(s) 533, 2569, 3989
EaeI YGGCCR 6 cut(s) 46, 171, 2427, 3639, 3840, 4009
EciI GGCGGA 3 cut(s) 13, 83, 107
Ecl136II GAGCTC 2 cut(s) 91, 412
Eco130I CCWWGG 5 cut(s) 1038, 1221, 1531, 2958, 3093
Eco147I AGGCCT 1 cut(s) 3066
Eco24I GRGCYC 2 cut(s) 93, 414
Eco31I GGTCTC 2 cut(s) 314, 1681
Eco32I GATATC 1 cut(s) 3055
Eco47I GGWCC 1 cut(s) 3952
Eco47III AGCGCT 1 cut(s) 2094
Eco53kI GAGCTC 2 cut(s) 91, 412
Eco57I CTGAAG 3 cut(s) 852, 966, 2989
Eco81I CCTNAGG 1 cut(s) 3018
Eco88I CYCGRG 2 cut(s) 86, 3306
EcoICRI GAGCTC 2 cut(s) 91, 412
EcoO109I RGGNCCY 1 cut(s) 3952
EcoRI GAATTC 2 cut(s) 976, 4014
EcoRII CCWGG 9 cut(s) 168, 183, 930, 947, 1005, 1308, 1739, 2612, 3798
EcoRV GATATC 1 cut(s) 3055
EcoT14I CCWWGG 5 cut(s) 1038, 1221, 1531, 2958, 3093
EcoT38I GRGCYC 2 cut(s) 93, 414
ErhI CCWWGG 5 cut(s) 1038, 1221, 1531, 2958, 3093
Esp3I CGTCTC 1 cut(s) 1271
FaqI GGGAC 3 cut(s) 469, 1221, 1296
FauI CCCGC 4 cut(s) 220, 715, 2921, 3825
FauNDI CATATG 1 cut(s) 1291
FbaI TGATCA 2 cut(s) 1167, 1698
FblI GTMKAC 2 cut(s) 2434, 2782
FokI GGATG 8 cut(s) 16, 2195, 2363, 2581, 3037, 3260, 3618, 3770
FriOI GRGCYC 2 cut(s) 93, 414
FspBI CTAG 6 cut(s) 347, 419, 1245, 1434, 3138, 3330
GlaI GCGC 6 cut(s) 1352, 1549, 1817, 1848, 2094, 3142
GsaI CCCAGC 1 cut(s) 1781
GsuI CTGGAG 6 cut(s) 298, 1028, 1140, 1677, 2391, 2715
HaeII RGCGCY 3 cut(s) 1850, 2096, 3144
HapII CCGG 5 cut(s) 256, 795, 2430, 2598, 3507
HgaI GACGC 4 cut(s) 327, 338, 1816, 2905
HhaI GCGC 6 cut(s) 1353, 1550, 1818, 1849, 2095, 3143
Hin1I GRCGYC 1 cut(s) 319
Hin6I GCGC 6 cut(s) 1351, 1548, 1816, 1847, 2093, 3141
HinP1I GCGC 6 cut(s) 1351, 1548, 1816, 1847, 2093, 3141
HincII GTYRAC 3 cut(s) 261, 2071, 2997
HindII GTYRAC 3 cut(s) 261, 2071, 2997
HpaI GTTAAC 1 cut(s) 2071
HpaII CCGG 5 cut(s) 256, 795, 2430, 2598, 3507
HphI GGTGA 3 cut(s) 226, 2581, 2602
Hpy99I CGWCG 1 cut(s) 539
HpyCH4IV ACGT 4 cut(s) 1337, 2902, 2932, 3984
HpySE526I ACGT 4 cut(s) 1337, 2902, 2932, 3984
Hsp92I GRCGYC 1 cut(s) 319
HspAI GCGC 6 cut(s) 1351, 1548, 1816, 1847, 2093, 3141
Kpn2I TCCGGA 1 cut(s) 2597
KpnI GGTACC 1 cut(s) 3942
Ksp22I TGATCA 2 cut(s) 1167, 1698
KspAI GTTAAC 1 cut(s) 2071
KspI CCGCGG 2 cut(s) 177, 3756
LguI GCTCTTC 2 cut(s) 402, 2807
LmnI GCTCC 6 cut(s) 96, 255, 589, 1049, 2230, 2906
LweI GCATC 4 cut(s) 673, 3015, 3596, 3961
MabI ACCWGGT 1 cut(s) 3798
MaeI CTAG 6 cut(s) 347, 419, 1245, 1434, 3138, 3330
MaeII ACGT 4 cut(s) 1337, 2902, 2932, 3984
MaeIII GTNAC 7 cut(s) 1267, 2651, 2933, 3034, 3325, 3469, 3991
MbiI CCGCTC 1 cut(s) 1636
MfeI CAATTG 2 cut(s) 3104, 3943
MflI RGATCY 1 cut(s) 3013
MhlI GDGCHC 7 cut(s) 93, 414, 551, 1343, 1370, 1398, 3723
MlsI TGGCCA 2 cut(s) 3641, 3842
MluNI TGGCCA 2 cut(s) 3641, 3842
MlyI GAGTC 8 cut(s) 1001, 1007, 1560, 1616, 1946, 3082, 3128, 4023
MmeI TCCRAC 6 cut(s) 549, 582, 712, 3784, 3819, 3839
Mox20I TGGCCA 2 cut(s) 3641, 3842
MroI TCCGGA 1 cut(s) 2597
MroXI GAANNNNTTC 1 cut(s) 3168
MscI TGGCCA 2 cut(s) 3641, 3842
MslI CAYNNNNRTG 2 cut(s) 3246, 3716
Msp20I TGGCCA 2 cut(s) 3641, 3842
MspA1I CMGCKG 6 cut(s) 176, 1165, 2006, 3755, 3767, 4035
MspCI CTTAAG 1 cut(s) 3077
MspI CCGG 5 cut(s) 256, 795, 2430, 2598, 3507
MunI CAATTG 2 cut(s) 3104, 3943
Mva1269I GAATGC 2 cut(s) 1900, 3787
MvaI CCWGG 9 cut(s) 170, 185, 932, 949, 1007, 1310, 1741, 2614, 3800
MvnI CGCG 7 cut(s) 176, 1351, 1816, 1818, 2487, 2916, 3755
NciI CCSGG 1 cut(s) 795
NdeI CATATG 1 cut(s) 1291
NlaIV GGNNCC 5 cut(s) 792, 942, 2226, 3538, 3940
NmeAIII GCCGAG 3 cut(s) 24, 2022, 2490
NmuCI GTSAC 3 cut(s) 1267, 3469, 3991
NspI RCATGY 5 cut(s) 532, 1987, 2875, 2941, 3566
PaeR7I CTCGAG 1 cut(s) 3306
PasI CCCWGGG 1 cut(s) 1740
PceI AGGCCT 1 cut(s) 3066
PciI ACATGT 4 cut(s) 528, 1983, 2937, 3562
PciSI GCTCTTC 2 cut(s) 402, 2807
PctI GAATGC 2 cut(s) 1900, 3787
PdmI GAANNNNTTC 1 cut(s) 3168
PfeI GAWTC 9 cut(s) 605, 798, 986, 1142, 1429, 1823, 2029, 2120, 2126
PfoI TCCNGGA 3 cut(s) 947, 1005, 1308
PinAI ACCGGT 1 cut(s) 3506
PleI GAGTC 8 cut(s) 1000, 1006, 1560, 1615, 1945, 3082, 3128, 4023
PpsI GAGTC 8 cut(s) 1000, 1006, 1560, 1615, 1945, 3082, 3128, 4023
Ppu21I YACGTR 2 cut(s) 1338, 2903
PpuMI RGGWCCY 1 cut(s) 3952
PscI ACATGT 4 cut(s) 528, 1983, 2937, 3562
PsiI TTATAA 1 cut(s) 1526
Psp124BI GAGCTC 2 cut(s) 93, 414
Psp1406I AACGTT 1 cut(s) 2932
Psp5II RGGWCCY 1 cut(s) 3952
Psp6I CCWGG 9 cut(s) 168, 183, 930, 947, 1005, 1308, 1739, 2612, 3798
PspFI CCCAGC 1 cut(s) 1777
PspGI CCWGG 9 cut(s) 168, 183, 930, 947, 1005, 1308, 1739, 2612, 3798
PspN4I GGNNCC 5 cut(s) 792, 942, 2226, 3538, 3940
PspPI GGNCC 2 cut(s) 3238, 3952
PspPPI RGGWCCY 1 cut(s) 3952
PsrI GAACNNNNNNTAC 2 cut(s) 3640, 3672
PstI CTGCAG 4 cut(s) 1141, 1759, 2011, 2397
PsuI RGATCY 1 cut(s) 3013
PvuII CAGCTG 4 cut(s) 1165, 2006, 3767, 4035
RseI CAYNNNNRTG 2 cut(s) 3246, 3716
SacI GAGCTC 2 cut(s) 93, 414
SacII CCGCGG 2 cut(s) 177, 3756
SapI GCTCTTC 2 cut(s) 402, 2807
Sau96I GGNCC 2 cut(s) 3238, 3952
ScaI AGTACT 1 cut(s) 855
SchI GAGTC 8 cut(s) 1001, 1007, 1560, 1616, 1946, 3082, 3128, 4023
SduI GDGCHC 7 cut(s) 93, 414, 551, 1343, 1370, 1398, 3723
SexAI ACCWGGT 1 cut(s) 3798
SfaNI GCATC 4 cut(s) 673, 3015, 3596, 3961
SfcI CTRYAG 4 cut(s) 1137, 1755, 2007, 2393
Sfr274I CTCGAG 1 cut(s) 3306
Sfr303I CCGCGG 2 cut(s) 177, 3756
SgrBI CCGCGG 2 cut(s) 177, 3756
SinI GGWCC 1 cut(s) 3952
SlaI CTCGAG 1 cut(s) 3306
SmiMI CAYNNNNRTG 2 cut(s) 3246, 3716
SmlI CTYRAG 6 cut(s) 1274, 1601, 2627, 2711, 3077, 3306
SmoI CTYRAG 6 cut(s) 1274, 1601, 2627, 2711, 3077, 3306
SseBI AGGCCT 1 cut(s) 3066
SspI AATATT 2 cut(s) 471, 3231
SspMI CTAG 6 cut(s) 347, 419, 1245, 1434, 3138, 3330
SstI GAGCTC 2 cut(s) 93, 414
StuI AGGCCT 1 cut(s) 3066
StyI CCWWGG 5 cut(s) 1038, 1221, 1531, 2958, 3093
TaiI ACGT 4 cut(s) 1340, 2905, 2935, 3987
TaqII GACCGA 1 cut(s) 2147
TatI WGTACW 6 cut(s) 853, 1207, 1460, 3420, 3427, 3433
TauI GCSGC 3 cut(s) 99, 176, 2729
TfiI GAWTC 9 cut(s) 605, 798, 986, 1142, 1429, 1823, 2029, 2120, 2126
TseFI GTSAC 3 cut(s) 1267, 3469, 3991
Tsp45I GTSAC 3 cut(s) 1267, 3469, 3991
TspGWI ACGGA 4 cut(s) 398, 523, 822, 1062
Vha464I CTTAAG 1 cut(s) 3077
VneI GTGCAC 1 cut(s) 3719
VpaK11BI GGWCC 1 cut(s) 3952
XapI RAATTY 6 cut(s) 976, 1596, 1675, 3453, 3550, 4014
XceI RCATGY 5 cut(s) 532, 1987, 2875, 2941, 3566
XhoI CTCGAG 1 cut(s) 3306
XmiI GTMKAC 2 cut(s) 2434, 2782
XmnI GAANNNNTTC 1 cut(s) 3168
XspI CTAG 6 cut(s) 347, 419, 1245, 1434, 3138, 3330
ZrmI AGTACT 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.