pycom16g11320

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001565
Physical Location & Seq
Forward (+)
7863612 .. 7866754
3143 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g11320.5

Sequence Viewer

Length: 714 bp
ATGTCTTGTTTAACGGCAAGAACAGGGCGGCAGAGACAGCGCTACGACGGTCATTTGAGGCTTGTTTCAGGGTGTATTCCTTATAAGTTTGAAAAGATTGCTGGCGGTTGCAATGGCAATATAGAGCAAAAATTGCTTGTTCTCATGATTTCTTCACCAAATCGGGATGATCTTGTGTTTCCAAAGGGCGGATGGGAGGATGACGAAACCATGAGGGAAGCTGCCTGTCGCGAGGCCTTGGAGGAAGCAGGAGTGAAGGGAGTTCTTCGTGATAATCCACTGGGAGTTTGGGAGTTTAGAAGCAAGAGCAAACAGAACAGCTGCAGTCTGCAAGGAGGTTGTAAAGGTTTCATGTTTGCAATGGAGGTGACTGAGGAGCTCGATTCCTGGCCCGAGCAGGCTAACTATGGAAGGAAATGGCTCACCATAGAAGAAGCATTCAGATTCTGTCGTTATGACTGGATGCGAGAAGCGCTAGGAAAACTTTTGACAACCCTATCAGAAAGCAGGGAGAATAACACAAGAAATGAACTGGAAGAACTTCCTTTGAGGCCAATCTCCGAAATTGGATCAGAACATCCAATGTCATCATCACCGGTGTGCTTTGCTAATCATTCCAGTATCCACAGCTTGCAGCTTAAAAATTTAACCAGAAAAATGAACTGTACAGGATTGATCAATGATCACAGGTCATGTAAAAATCCCTTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

238

Amino Acids

27.0

Weight (kDa)

6.95

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 84
AccII CGCG 1 cut(s) 231
AciI CCGC 3 cut(s) 28, 105, 189
AclWI GGATC 1 cut(s) 577
AcsI RAATTY 1 cut(s) 643
AfaI GTAC 1 cut(s) 667
AfeI AGCGCT 2 cut(s) 41, 474
AfiI CCNNNNNNNGG 1 cut(s) 188
AgeI ACCGGT 1 cut(s) 595
AgsI TTSAA 1 cut(s) 92
AjnI CCWGG 1 cut(s) 386
AleI CACNNNNGTG 1 cut(s) 598
AluBI AGCT 5 cut(s) 221, 321, 379, 630, 637
AluI AGCT 5 cut(s) 221, 321, 379, 630, 637
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 1 cut(s) 577
AlwNI CAGNNNCTG 1 cut(s) 447
Ama87I CYCGRG 1 cut(s) 392
Aor51HI AGCGCT 2 cut(s) 41, 474
AoxI GGCC 3 cut(s) 234, 389, 551
ApeKI GCWGC 3 cut(s) 221, 321, 634
ApoI RAATTY 1 cut(s) 643
AsiGI ACCGGT 1 cut(s) 595
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 2 cut(s) 42, 475
AspS9I GGNCC 1 cut(s) 390
AsuHPI GGTGA 4 cut(s) 147, 379, 415, 585
AvaI CYCGRG 1 cut(s) 392
BanII GRGCYC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 208, 308, 646
BccI CCATC 1 cut(s) 186
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 388
BciVI GTATCC 1 cut(s) 632
BclI TGATCA 2 cut(s) 675, 682
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 1 cut(s) 476
BfmI CTRYAG 1 cut(s) 322
BfoI RGCGCY 2 cut(s) 43, 476
BfuI GTATCC 1 cut(s) 632
BisI GCNGC 4 cut(s) 29, 222, 322, 635
BlsI GCNGC 4 cut(s) 30, 223, 323, 636
Bme1390I CCNGG 1 cut(s) 388
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 390
BmrFI CCNGG 1 cut(s) 388
BmrI ACTGGG 1 cut(s) 290
BmsI GCATC 1 cut(s) 453
BmuI ACTGGG 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 237
BsaWI WCCGGW 1 cut(s) 595
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 4 cut(s) 285, 464, 537, 618
Bse3DI GCAATG 2 cut(s) 118, 366
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 5 cut(s) 172, 197, 205, 468, 577
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 2 cut(s) 118, 366
BseMII CTCAG 1 cut(s) 363
BseNI ACTGG 4 cut(s) 285, 464, 537, 618
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 3 cut(s) 208, 308, 646
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 3 cut(s) 236, 391, 553
BshTI ACCGGT 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 28
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 3 cut(s) 236, 391, 553
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 381
Bsp1407I TGTACA 1 cut(s) 665
Bsp143I GATC 4 cut(s) 169, 569, 675, 682
Bsp68I TCGCGA 1 cut(s) 231
BspACI CCGC 3 cut(s) 28, 105, 189
BspANI GGCC 3 cut(s) 236, 391, 553
BspCNI CTCAG 1 cut(s) 364
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 144
BspMAI CTGCAG 1 cut(s) 326
BspPI GGATC 1 cut(s) 577
BsrDI GCAATG 2 cut(s) 118, 366
BsrFI RCCGGY 1 cut(s) 595
BsrGI TGTACA 1 cut(s) 665
BsrI ACTGG 4 cut(s) 285, 464, 537, 618
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 4 cut(s) 169, 569, 675, 682
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 50, 665
BstAPI GCANNNNNTGC 1 cut(s) 133
BstAUI TGTACA 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 103, 399, 632
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 5 cut(s) 172, 197, 205, 468, 577
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 2 cut(s) 43, 476
BstHHI GCGC 2 cut(s) 42, 475
BstKTI GATC 4 cut(s) 172, 572, 678, 685
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 4 cut(s) 169, 569, 675, 682
BstMWI GCNNNNNNNGC 3 cut(s) 37, 133, 472
BstNI CCWGG 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 386
BstSFI CTRYAG 1 cut(s) 322
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 3 cut(s) 208, 308, 646
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 3 cut(s) 236, 391, 553
BtsCI GGATG 5 cut(s) 172, 197, 205, 468, 577
BtsIMutI CAGTG 1 cut(s) 278
BtuMI TCGCGA 1 cut(s) 231
Cac8I GCNNGC 3 cut(s) 103, 399, 632
CaiI CAGNNNCTG 1 cut(s) 447
CciI TCATGA 1 cut(s) 144
CfoI GCGC 2 cut(s) 42, 475
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 390
Csp6I GTAC 1 cut(s) 666
CspAI ACCGGT 1 cut(s) 595
CviAII CATG 4 cut(s) 145, 211, 352, 693
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 1 cut(s) 372
DpnI GATC 4 cut(s) 171, 571, 677, 684
DpnII GATC 4 cut(s) 169, 569, 675, 682
EciI GGCGGA 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 237
Eco147I AGGCCT 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 381
Eco47III AGCGCT 2 cut(s) 41, 474
Eco53kI GAGCTC 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 4 cut(s) 148, 214, 355, 696
FaiI YATR 9 cut(s) 84, 122, 146, 212, 353, 408, 428, 456, 694
FatI CATG 4 cut(s) 144, 210, 351, 692
FbaI TGATCA 2 cut(s) 675, 682
Fnu4HI GCNGC 4 cut(s) 29, 222, 322, 635
FokI GGATG 5 cut(s) 179, 204, 212, 475, 564
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 4 cut(s) 29, 222, 322, 635
FspBI CTAG 1 cut(s) 476
GlaI GCGC 2 cut(s) 41, 474
GluI GCNGC 4 cut(s) 29, 222, 322, 635
HaeII RGCGCY 2 cut(s) 43, 476
HaeIII GGCC 3 cut(s) 236, 391, 553
HapII CCGG 1 cut(s) 596
HhaI GCGC 2 cut(s) 42, 475
Hin1II CATG 4 cut(s) 148, 214, 355, 696
Hin6I GCGC 2 cut(s) 40, 473
HinP1I GCGC 2 cut(s) 40, 473
HinfI GANTC 2 cut(s) 383, 444
HpaII CCGG 1 cut(s) 596
HphI GGTGA 4 cut(s) 147, 379, 415, 585
Hpy188I TCNGA 5 cut(s) 443, 502, 562, 574, 713
Hpy188III TCNNGA 4 cut(s) 145, 164, 230, 269
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 250, 405
HpyCH4III ACNGT 2 cut(s) 50, 665
HpyCH4V TGCA 5 cut(s) 111, 324, 331, 359, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 133, 472
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 4 cut(s) 148, 214, 355, 696
HspAI GCGC 2 cut(s) 40, 473
Ksp22I TGATCA 2 cut(s) 675, 682
Kzo9I GATC 4 cut(s) 169, 569, 675, 682
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 3 cut(s) 208, 308, 646
LweI GCATC 1 cut(s) 453
MaeI CTAG 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 367
MalI GATC 4 cut(s) 171, 571, 677, 684
MboI GATC 4 cut(s) 169, 569, 675, 682
MboII GAAGA 4 cut(s) 144, 257, 443, 548
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 3 cut(s) 131, 564, 643
MnlI CCTC 9 cut(s) 51, 190, 207, 226, 235, 329, 358, 367, 543
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 3 cut(s) 11, 639, 647
MslI CAYNNNNRTG 1 cut(s) 598
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 1 cut(s) 388
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 3 cut(s) 37, 133, 472
NdeII GATC 4 cut(s) 169, 569, 675, 682
NlaIII CATG 4 cut(s) 148, 214, 355, 696
NmuCI GTSAC 1 cut(s) 367
NruI TCGCGA 1 cut(s) 231
OliI CACNNNNGTG 1 cut(s) 598
PagI TCATGA 1 cut(s) 144
PceI AGGCCT 1 cut(s) 236
PctI GAATGC 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 2 cut(s) 383, 444
PinAI ACCGGT 1 cut(s) 595
PkrI GCNGC 4 cut(s) 30, 223, 323, 636
PsiI TTATAA 1 cut(s) 84
Psp124BI GAGCTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 386
PspGI CCWGG 1 cut(s) 386
PspPI GGNCC 1 cut(s) 390
PstI CTGCAG 1 cut(s) 326
PstNI CAGNNNCTG 1 cut(s) 447
PvuII CAGCTG 1 cut(s) 321
RruI TCGCGA 1 cut(s) 231
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
RseI CAYNNNNRTG 1 cut(s) 598
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 3 cut(s) 11, 639, 647
SatI GCNGC 4 cut(s) 29, 222, 322, 635
Sau3AI GATC 4 cut(s) 169, 569, 675, 682
Sau96I GGNCC 1 cut(s) 390
ScrFI CCNGG 1 cut(s) 388
SduI GDGCHC 1 cut(s) 381
SetI ASST 9 cut(s) 223, 323, 340, 349, 369, 381, 632, 639, 692
SfaNI GCATC 1 cut(s) 453
SfcI CTRYAG 1 cut(s) 322
SgrAI CRCCGGYG 1 cut(s) 595
SmiMI CAYNNNNRTG 1 cut(s) 598
Sse9I AATT 3 cut(s) 131, 564, 643
SseBI AGGCCT 1 cut(s) 236
SsiI CCGC 3 cut(s) 28, 105, 189
SspMI CTAG 1 cut(s) 476
SstI GAGCTC 1 cut(s) 381
StuI AGGCCT 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 386
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 2 cut(s) 50, 665
TaqI TCGA 1 cut(s) 381
TasI AATT 3 cut(s) 131, 564, 643
TatI WGTACW 1 cut(s) 665
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 2 cut(s) 383, 444
Tru1I TTAA 3 cut(s) 11, 639, 647
Tru9I TTAA 3 cut(s) 11, 639, 647
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 221, 321, 634
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 3 cut(s) 340, 543, 674
TspRI CASTG 1 cut(s) 285
XapI RAATTY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 2 cut(s) 189, 285
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.