Rroxscaffold_5G00370270

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001565
Physical Location & Seq
Reverse (-)
51910748 .. 51914697
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370270.1

Sequence Viewer

Length: 693 bp
ATGTCTTGTTTGACAGCCAGAACAGGGCGGCAGAGACAGCGGTACGAGGATCAATTGAGGCTTGTTTCAGGATGTATACCTTATAGGTTTGAAGAGACAGTAGGGAGTAGCAATTGCAATGTAGAGAAAAAGTTGCTTGTTCTCATGATTTCTTCACCAGATCGAGATGATCTTGTCTTTCCAAAGGGAGGATGGGAGAATGACGAAACCATGCATGAAGCTGCTTGTCGCGAGGCCTTAGAAGAAGCAGGAGTGAAGGGAGTTCTTGGTGTAGGTTTCTCTTCCCTGCTTGGTAACAGGCATGGACTCCTTGTCACTGAAACTCCACTAGGACAATGGGAGTTCAGAAGCAAGAGCAAACAGAACAATGGCAGCCTGCAAGGTGGTTGTAGAGGTTACATGTTTGCACTAAGGGTTACTGAAGAGCTCGACTCCTGGCCCGAGCAGACCATCTACGGAAGGAAATGGGTTACCGTAGAAGACGCATTCAAGTTCTGTCGGTATGACTGGATGCGAGAAGCTCTTAGAAAGCTTTTGATAGCACTCTCAGAGAACACGGAAAATGGCCCGAGAGAAGAACTGGATGGACTTCCTTTGAGGCCAGTTATCTCAGAAGTCGGATCAGAGCATCAAATATCATCACCATCCGGTTGCTTTGCTAGTCATTCTAGTGTTCAGCAGCTCGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

230

Amino Acids

25.79

Weight (kDa)

5.53

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 40 - 177 1.4e-09 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 76
AccII CGCG 1 cut(s) 231
AciI CCGC 2 cut(s) 28, 40
AclWI GGATC 2 cut(s) 57, 628
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 2 cut(s) 24, 188
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 2 cut(s) 92, 490
AhdI GACNNNNNGTC 1 cut(s) 311
AjnI CCWGG 1 cut(s) 434
AluBI AGCT 6 cut(s) 221, 427, 521, 532, 682, 689
AluI AGCT 6 cut(s) 221, 427, 521, 532, 682, 689
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 28, 89
AlwI GGATC 2 cut(s) 57, 628
Ama87I CYCGRG 2 cut(s) 440, 568
AoxI GGCC 4 cut(s) 234, 437, 565, 599
ApeKI GCWGC 4 cut(s) 221, 372, 679, 686
AspS9I GGNCC 2 cut(s) 438, 566
AsuHPI GGTGA 2 cut(s) 147, 633
AvaI CYCGRG 2 cut(s) 440, 568
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 208, 384
BccI CCATC 4 cut(s) 186, 458, 578, 652
BciT130I CCWGG 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 28, 89
BfaI CTAG 3 cut(s) 329, 660, 669
BisI GCNGC 5 cut(s) 29, 222, 373, 680, 687
BlsI GCNGC 5 cut(s) 30, 223, 374, 681, 688
Bme1390I CCNGG 1 cut(s) 436
BmeRI GACNNNNNGTC 1 cut(s) 311
BmeT110I CYCGRG 2 cut(s) 440, 568
BmgT120I GGNCC 2 cut(s) 438, 566
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 2 cut(s) 501, 637
BpiI GAAGAC 1 cut(s) 486
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BsaWI WCCGGW 1 cut(s) 647
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 188
Bse1I ACTGG 3 cut(s) 512, 585, 602
Bse3DI GCAATG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 436
BseGI GGATG 5 cut(s) 77, 197, 516, 589, 644
BseLI CCNNNNNNNGG 2 cut(s) 24, 188
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 2 cut(s) 561, 624
BseNI ACTGG 3 cut(s) 512, 585, 602
BseXI GCAGC 2 cut(s) 208, 384
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 236, 439, 567, 601
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 2 cut(s) 440, 568
BsiSI CCGG 1 cut(s) 648
BslI CCNNNNNNNGG 2 cut(s) 24, 188
BsmAI GTCTC 2 cut(s) 28, 89
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 4 cut(s) 236, 439, 567, 601
BsoBI CYCGRG 2 cut(s) 440, 568
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 4 cut(s) 49, 160, 169, 620
Bsp68I TCGCGA 1 cut(s) 231
BspACI CCGC 2 cut(s) 28, 40
BspANI GGCC 4 cut(s) 236, 439, 567, 601
BspCNI CTCAG 2 cut(s) 560, 623
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 144
BspPI GGATC 2 cut(s) 57, 628
BspQI GCTCTTC 1 cut(s) 417
BsrDI GCAATG 1 cut(s) 124
BsrI ACTGG 3 cut(s) 512, 585, 602
BssMI GATC 4 cut(s) 49, 160, 169, 620
BssNAI GTATAC 1 cut(s) 77
Bst1107I GTATAC 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 100, 475
Bst6I CTCTTC 3 cut(s) 87, 286, 417
BstC8I GCNNGC 2 cut(s) 377, 684
BstDEI CTNAG 5 cut(s) 238, 410, 524, 547, 610
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 5 cut(s) 77, 197, 516, 589, 644
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 4 cut(s) 52, 163, 172, 623
BstMAI GTCTC 2 cut(s) 28, 89
BstMBI GATC 4 cut(s) 49, 160, 169, 620
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 1 cut(s) 403
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 434
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 208, 384
BstV2I GAAGAC 1 cut(s) 486
BstZ17I GTATAC 1 cut(s) 77
BsuRI GGCC 4 cut(s) 236, 439, 567, 601
BtsCI GGATG 5 cut(s) 77, 197, 516, 589, 644
BtsIMutI CAGTG 1 cut(s) 315
BtuMI TCGCGA 1 cut(s) 231
Cac8I GCNNGC 2 cut(s) 377, 684
CciI TCATGA 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 438, 566
CseI GACGC 1 cut(s) 491
Csp6I GTAC 1 cut(s) 43
CviAII CATG 5 cut(s) 145, 211, 215, 302, 400
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 5 cut(s) 238, 410, 524, 547, 610
DpnI GATC 4 cut(s) 51, 162, 171, 622
DpnII GATC 4 cut(s) 49, 160, 169, 620
DriI GACNNNNNGTC 1 cut(s) 311
Eam1104I CTCTTC 3 cut(s) 87, 286, 417
Eam1105I GACNNNNNGTC 1 cut(s) 311
EarI CTCTTC 3 cut(s) 87, 286, 417
Ecl136II GAGCTC 1 cut(s) 427
Eco147I AGGCCT 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 2 cut(s) 440, 568
Eco91I GGTNACC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 427
EcoO65I GGTNACC 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 434
EcoT22I ATGCAT 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 429
FaeI CATG 5 cut(s) 148, 214, 218, 305, 403
FaiI YATR 8 cut(s) 77, 84, 146, 212, 216, 303, 401, 504
FatI CATG 5 cut(s) 144, 210, 214, 301, 399
FblI GTMKAC 1 cut(s) 76
Fnu4HI GCNGC 5 cut(s) 29, 222, 373, 680, 687
FokI GGATG 5 cut(s) 84, 204, 523, 596, 631
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 29, 222, 373, 680, 687
FspBI CTAG 3 cut(s) 329, 660, 669
GluI GCNGC 5 cut(s) 29, 222, 373, 680, 687
HaeIII GGCC 4 cut(s) 236, 439, 567, 601
HapII CCGG 1 cut(s) 648
HgaI GACGC 1 cut(s) 491
Hin1II CATG 5 cut(s) 148, 214, 218, 305, 403
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 2 cut(s) 306, 431
HpaII CCGG 1 cut(s) 648
HphI GGTGA 2 cut(s) 147, 633
Hpy166II GTNNAC 1 cut(s) 77
Hpy188I TCNGA 5 cut(s) 347, 550, 613, 620, 625
Hpy188III TCNNGA 4 cut(s) 69, 145, 164, 230
Hpy8I GTNNAC 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 250, 453
HpyCH4III ACNGT 2 cut(s) 100, 475
HpyCH4V TGCA 4 cut(s) 117, 214, 379, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 5 cut(s) 238, 410, 524, 547, 610
Hsp92II CATG 5 cut(s) 148, 214, 218, 305, 403
Kzo9I GATC 4 cut(s) 49, 160, 169, 620
LguI GCTCTTC 1 cut(s) 417
Lsp1109I GCAGC 2 cut(s) 208, 384
LweI GCATC 2 cut(s) 501, 637
MaeI CTAG 3 cut(s) 329, 660, 669
MaeIII GTNAC 5 cut(s) 293, 313, 395, 415, 469
MalI GATC 4 cut(s) 51, 162, 171, 622
MboI GATC 4 cut(s) 49, 160, 169, 620
MboII GAAGA 7 cut(s) 104, 144, 254, 273, 434, 491, 587
MfeI CAATTG 2 cut(s) 53, 112
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 2 cut(s) 53, 112
MlyI GAGTC 2 cut(s) 300, 425
MmeI TCCRAC 1 cut(s) 598
MnlI CCTC 6 cut(s) 40, 51, 182, 226, 386, 591
Mph1103I ATGCAT 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 436
MunI CAATTG 2 cut(s) 53, 112
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeII GATC 4 cut(s) 49, 160, 169, 620
NlaIII CATG 5 cut(s) 148, 214, 218, 305, 403
NmuCI GTSAC 1 cut(s) 313
NruI TCGCGA 1 cut(s) 231
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 403
PagI TCATGA 1 cut(s) 144
PceI AGGCCT 1 cut(s) 236
PciI ACATGT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 417
PctI GAATGC 1 cut(s) 485
PkrI GCNGC 5 cut(s) 30, 223, 374, 681, 688
PleI GAGTC 2 cut(s) 300, 425
PpsI GAGTC 2 cut(s) 300, 425
PscI ACATGT 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 434
PspEI GGTNACC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 434
PspPI GGNCC 2 cut(s) 438, 566
RruI TCGCGA 1 cut(s) 231
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 669
SacI GAGCTC 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 417
SatI GCNGC 5 cut(s) 29, 222, 373, 680, 687
Sau3AI GATC 4 cut(s) 49, 160, 169, 620
Sau96I GGNCC 2 cut(s) 438, 566
SchI GAGTC 2 cut(s) 300, 425
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 2 cut(s) 501, 637
SmiMI CAYNNNNRTG 1 cut(s) 669
Sse9I AATT 2 cut(s) 53, 112
SseBI AGGCCT 1 cut(s) 236
SsiI CCGC 2 cut(s) 28, 40
SspMI CTAG 3 cut(s) 329, 660, 669
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 434
TaaI ACNGT 2 cut(s) 100, 475
TaqI TCGA 2 cut(s) 163, 429
TasI AATT 2 cut(s) 53, 112
TauI GCSGC 1 cut(s) 31
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 4 cut(s) 221, 372, 679, 686
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 2 cut(s) 471, 572
TspRI CASTG 1 cut(s) 322
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 2 cut(s) 189, 333
XmiI GTMKAC 1 cut(s) 76
XspI CTAG 3 cut(s) 329, 660, 669
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.