pycom16g15750

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003646
Physical Location & Seq
Forward (+)
11692478 .. 11695905
3428 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGGGGAAGAAGAAGAGAGCCGAATCAGAAGCAGAAGCACAACCAGAAGATAACAAAGTCGACATTGCCAGTGGAGATTCTCATAGGAAAGAGAAGAAGAAGAAGCACAAAGTCGACGGGGCCGCCGTGATACCCACCGTGAGCGTCGCCGTCCCCGGTTCAATCATCGAGAACACTCAATCCCTCGAACTCGCCACCAGATTGGCCAGTCAGATTGCTCGTGCTGCGACGATTTTCCGAATCGACGAGGTGGTGATATTTGACAATAAGAGTGAGTCTGGCTCATTGCCAGAAGCTGATTCGGATGAGAATGAAAGTGGTGCTGCTTTTCTTGTACGAATCTTGAGGTACCTTGAGACACCTCAGTATTTGAGAAAAGCTCTGTTTCCGAAACACAACAGCTTAAGATATGTGGGTATGTTGCCCCCGCTTGATGCTCCGCACCATCTGCGAAAACATGAATGGGGTCCGTTTCGGGAAGGTGTCACACTGAAAGAAAGACCTCCCAACGGAACATTAGTTGATGTGGGCTTGAGTAAGAATGTCATTGTTGATCAAGTACTTGATCCTGGAACCAGAGTTACTGTGGCTATGGGGGCCAGTTGGAACTTGGATGCTGGTAATGATTTTATATCTCACGGCAGGTTGTCTCATCCTCAAAGCCTAGGGAAGAAGCAGGAACTTATTGGGGGTACAAAATATGGCGTTTCTTTACCAACTTCTGTTATTCAATCACAGGGAGGCTATGATCATTCAATTGGTACATCAGAGCACGGTCAGATTATTAATTCCTATGATCTCACTATACCTACCTTCAGGCATCTATTGATTGCTTTTGGTGGACTTGCTGGGTTGGAAGAGAGCGTTGAAGAGGATGCTAACTTAAAGGCAAAGAATGTGCGAGAGATATTTGATTTGTATTTGAACACATGCCCGCATCAAGGGAGTCGAACAATTCGAACAGAGCCGAGCATTGAAGAGAACTCGTTGAAGGACATTAAGAATTCCCACAGTTGTCAGGATCACAGGGAACCTGAAATCAATTCACGATTCTTCCTCATCGTAACTTGGCTTCTAACGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

362

Amino Acids

39.85

Weight (kDa)

6.28

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 46 - 323 3e-86 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 633
Acc65I GGTACC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 348
AccI GTMKAC 2 cut(s) 60, 114
AciI CCGC 4 cut(s) 123, 428, 440, 935
AclWI GGATC 2 cut(s) 560, 1029
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 1003
AcuI CTGAAG 1 cut(s) 799
AfaI GTAC 5 cut(s) 336, 350, 561, 694, 763
AfiI CCNNNNNNNGG 2 cut(s) 509, 941
AflII CTTAAG 1 cut(s) 403
AgsI TTSAA 7 cut(s) 162, 731, 756, 869, 925, 977, 991
AjnI CCWGG 1 cut(s) 568
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
AluBI AGCT 3 cut(s) 296, 380, 402
AluI AGCT 3 cut(s) 296, 380, 402
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 2 cut(s) 350, 654
AlwI GGATC 2 cut(s) 560, 1029
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 3 cut(s) 120, 204, 597
ApeKI GCWGC 2 cut(s) 224, 323
ApoI RAATTY 1 cut(s) 1003
AseI ATTAAT 1 cut(s) 786
Asp718I GGTACC 1 cut(s) 348
AspA2I CCTAGG 1 cut(s) 664
AspS9I GGNCC 3 cut(s) 120, 467, 597
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 265
AsuII TTCGAA 1 cut(s) 958
AvaII GGWCC 1 cut(s) 467
AvrII CCTAGG 1 cut(s) 664
BalI TGGCCA 1 cut(s) 206
BanI GGYRCC 1 cut(s) 348
BarI GAAGNNNNNNTAC 1 cut(s) 1056
BauI CACGAG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 2 cut(s) 211, 310
BccI CCATC 1 cut(s) 453
BceAI ACGGC 3 cut(s) 110, 134, 655
BciT130I CCWGG 1 cut(s) 570
BclI TGATCA 2 cut(s) 553, 748
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 350, 654
BfaI CTAG 1 cut(s) 665
BfrI CTTAAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 633
BisI GCNGC 3 cut(s) 123, 225, 324
BlnI CCTAGG 1 cut(s) 664
BlsI GCNGC 3 cut(s) 124, 226, 325
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 156, 570
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 3 cut(s) 120, 467, 597
BmiI GGNNCC 6 cut(s) 121, 350, 468, 574, 598, 1032
BmrFI CCNGG 2 cut(s) 156, 570
BmsI GCATC 5 cut(s) 424, 604, 829, 865, 946
BplI GAGNNNNNCTC 4 cut(s) 266, 298, 364, 396
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 3 cut(s) 364, 374, 553
BpuMI CCSGG 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 154, 664
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 941
Bse1I ACTGG 3 cut(s) 69, 207, 600
Bse3DI GCAATG 2 cut(s) 63, 284
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 154, 664
BseGI GGATG 4 cut(s) 310, 619, 652, 880
BseLI CCNNNNNNNGG 2 cut(s) 509, 941
BseMI GCAATG 2 cut(s) 63, 284
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 3 cut(s) 69, 207, 600
BseXI GCAGC 2 cut(s) 211, 310
BseYI CCCAGC 1 cut(s) 848
BshFI GGCC 3 cut(s) 122, 206, 599
BshNI GGYRCC 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 509, 941
BsmAI GTCTC 2 cut(s) 350, 654
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 3 cut(s) 122, 206, 599
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 1 cut(s) 774
Bsp143I GATC 5 cut(s) 553, 565, 748, 796, 1021
BspACI CCGC 4 cut(s) 123, 428, 440, 935
BspANI GGCC 3 cut(s) 122, 206, 599
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 1082
BspLI GGNNCC 6 cut(s) 121, 350, 468, 574, 598, 1032
BspMI ACCTGC 1 cut(s) 633
BspPI GGATC 2 cut(s) 560, 1029
BspT104I TTCGAA 1 cut(s) 958
BspT107I GGYRCC 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 63, 284
BsrI ACTGG 3 cut(s) 69, 207, 600
BssECI CCNNGG 2 cut(s) 154, 664
BssMI GATC 5 cut(s) 553, 565, 748, 796, 1021
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 664
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 4 cut(s) 139, 586, 776, 1013
Bst6I CTCTTC 4 cut(s) 8, 852, 864, 972
BstAFI CTTAAG 1 cut(s) 403
BstAPI GCANNNNNTGC 1 cut(s) 448
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 1 cut(s) 935
BstDEI CTNAG 1 cut(s) 363
BstF5I GGATG 4 cut(s) 310, 619, 652, 880
BstKTI GATC 5 cut(s) 556, 568, 751, 799, 1024
BstMAI GTCTC 2 cut(s) 350, 654
BstMBI GATC 5 cut(s) 553, 565, 748, 796, 1021
BstMWI GCNNNNNNNGC 3 cut(s) 224, 448, 596
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 2 cut(s) 154, 568
BstV1I GCAGC 2 cut(s) 211, 310
BstXI CCANNNNNNTGG 1 cut(s) 202
BsuRI GGCC 3 cut(s) 122, 206, 599
BtsCI GGATG 4 cut(s) 310, 619, 652, 880
BtsIMutI CAGTG 2 cut(s) 76, 488
BveI ACCTGC 1 cut(s) 633
Cac8I GCNNGC 1 cut(s) 935
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 1082
Cfr13I GGNCC 3 cut(s) 120, 467, 597
CseI GACGC 1 cut(s) 133
Csp6I GTAC 5 cut(s) 335, 349, 560, 693, 762
CviAII CATG 3 cut(s) 458, 930, 1083
CviQI GTAC 5 cut(s) 335, 349, 560, 693, 762
DdeI CTNAG 1 cut(s) 363
DpnI GATC 5 cut(s) 555, 567, 750, 798, 1023
DpnII GATC 5 cut(s) 553, 565, 748, 796, 1021
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 4 cut(s) 8, 852, 864, 972
EarI CTCTTC 4 cut(s) 8, 852, 864, 972
Eco130I CCWWGG 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 799
EcoRI GAATTC 1 cut(s) 1003
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 664
FaeI CATG 3 cut(s) 461, 933, 1086
FaqI GGGAC 1 cut(s) 137
FatI CATG 3 cut(s) 457, 929, 1082
FauI CCCGC 2 cut(s) 435, 942
FbaI TGATCA 2 cut(s) 553, 748
FblI GTMKAC 2 cut(s) 60, 114
Fnu4HI GCNGC 3 cut(s) 123, 225, 324
FokI GGATG 4 cut(s) 317, 626, 639, 887
Fsp4HI GCNGC 3 cut(s) 123, 225, 324
FspBI CTAG 1 cut(s) 665
GluI GCNGC 3 cut(s) 123, 225, 324
GsaI CCCAGC 1 cut(s) 852
HaeIII GGCC 3 cut(s) 122, 206, 599
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 133
Hin1II CATG 3 cut(s) 461, 933, 1086
HincII GTYRAC 2 cut(s) 61, 115
HindII GTYRAC 2 cut(s) 61, 115
HinfI GANTC 8 cut(s) 23, 77, 240, 275, 299, 339, 946, 1050
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 3 cut(s) 61, 115, 842
Hpy188I TCNGA 7 cut(s) 28, 213, 239, 304, 390, 769, 780
Hpy188III TCNNGA 6 cut(s) 169, 343, 476, 1019, 1047, 1083
Hpy8I GTNNAC 3 cut(s) 61, 115, 842
Hpy99I CGWCG 4 cut(s) 119, 149, 232, 248
HpyAV CCTTC 3 cut(s) 473, 823, 985
HpyCH4III ACNGT 4 cut(s) 139, 586, 776, 1013
HpyCH4IV ACGT 1 cut(s) 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 448, 596
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 1079
Hsp92II CATG 3 cut(s) 461, 933, 1086
KpnI GGTACC 1 cut(s) 352
Ksp22I TGATCA 2 cut(s) 553, 748
Kzo9I GATC 5 cut(s) 553, 565, 748, 796, 1021
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 211, 310
LweI GCATC 5 cut(s) 424, 604, 829, 865, 946
MaeI CTAG 1 cut(s) 665
MaeII ACGT 1 cut(s) 1079
MaeIII GTNAC 3 cut(s) 484, 580, 1063
MalI GATC 5 cut(s) 555, 567, 750, 798, 1023
MboI GATC 5 cut(s) 553, 565, 748, 796, 1021
MfeI CAATTG 1 cut(s) 756
MhlI GDGCHC 1 cut(s) 774
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 5 cut(s) 756, 787, 954, 1003, 1042
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 2 cut(s) 284, 955
MmeI TCCRAC 2 cut(s) 584, 834
MnlI CCTC 9 cut(s) 194, 241, 339, 372, 513, 666, 734, 865, 1067
Mox20I TGGCCA 1 cut(s) 206
MscI TGGCCA 1 cut(s) 206
MseI TTAA 4 cut(s) 404, 786, 884, 999
Msp20I TGGCCA 1 cut(s) 206
MspCI CTTAAG 1 cut(s) 403
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 156, 570
MunI CAATTG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 570
MwoI GCNNNNNNNGC 3 cut(s) 224, 448, 596
NciI CCSGG 1 cut(s) 156
NdeII GATC 5 cut(s) 553, 565, 748, 796, 1021
NlaIII CATG 3 cut(s) 461, 933, 1086
NlaIV GGNNCC 6 cut(s) 121, 350, 468, 574, 598, 1032
NmeAIII GCCGAG 1 cut(s) 993
NmuCI GTSAC 1 cut(s) 484
NspI RCATGY 1 cut(s) 933
NspV TTCGAA 1 cut(s) 958
PagI TCATGA 1 cut(s) 1082
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 955
PfeI GAWTC 6 cut(s) 23, 77, 240, 299, 339, 1050
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 3 cut(s) 124, 226, 325
PleI GAGTC 2 cut(s) 283, 954
PpsI GAGTC 2 cut(s) 283, 954
PshBI ATTAAT 1 cut(s) 786
Psp6I CCWGG 1 cut(s) 568
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 6 cut(s) 121, 350, 468, 574, 598, 1032
PspPI GGNCC 3 cut(s) 120, 467, 597
PsrI GAACNNNNNNTAC 2 cut(s) 565, 597
PstNI CAGNNNCTG 1 cut(s) 296
RsaI GTAC 5 cut(s) 336, 350, 561, 694, 763
RsaNI GTAC 5 cut(s) 335, 349, 560, 693, 762
SalI GTCGAC 2 cut(s) 59, 113
SaqAI TTAA 4 cut(s) 404, 786, 884, 999
SatI GCNGC 3 cut(s) 123, 225, 324
Sau3AI GATC 5 cut(s) 553, 565, 748, 796, 1021
Sau96I GGNCC 3 cut(s) 120, 467, 597
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 2 cut(s) 284, 955
ScrFI CCNGG 2 cut(s) 156, 570
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 5 cut(s) 424, 604, 829, 865, 946
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 1 cut(s) 467
SmlI CTYRAG 4 cut(s) 343, 353, 403, 532
SmoI CTYRAG 4 cut(s) 343, 353, 403, 532
Sse9I AATT 5 cut(s) 756, 787, 954, 1003, 1042
SsiI CCGC 4 cut(s) 123, 428, 440, 935
SspMI CTAG 1 cut(s) 665
StyD4I CCNGG 2 cut(s) 154, 568
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 4 cut(s) 139, 586, 776, 1013
TaiI ACGT 1 cut(s) 1082
TaqI TCGA 7 cut(s) 60, 114, 168, 186, 243, 949, 958
TasI AATT 5 cut(s) 756, 787, 954, 1003, 1042
TatI WGTACW 1 cut(s) 559
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 6 cut(s) 23, 77, 240, 299, 339, 1050
Tru1I TTAA 4 cut(s) 404, 786, 884, 999
Tru9I TTAA 4 cut(s) 404, 786, 884, 999
TscAI CASTG 2 cut(s) 76, 495
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 2 cut(s) 224, 323
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 2 cut(s) 327, 474
TspGWI ACGGA 2 cut(s) 459, 525
TspRI CASTG 2 cut(s) 76, 495
Vha464I CTTAAG 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 467
VspI ATTAAT 1 cut(s) 786
XapI RAATTY 1 cut(s) 1003
XceI RCATGY 1 cut(s) 933
XcmI CCANNNNNNNNNTGG 2 cut(s) 583, 607
XmaJI CCTAGG 1 cut(s) 664
XmiI GTMKAC 2 cut(s) 60, 114
XspI CTAG 1 cut(s) 665
ZrmI AGTACT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.