pycom16g22820

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001671
Physical Location & Seq
Forward (+)
23185831 .. 23190736
4906 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1509 bp
ATGAGAGCAATTGCTCGATCTTTTCACCAATGTAGATTGGCTTTGTGCCATCAAGCATGTCATTACTCAAGTGGTTTTGTAACAAGGCAGAAGCTGTTGACAAACACCCGTGCTGTGTTTGCTTATCAACTCGCTAAAAATGCCGAATTCGATAGACTTCAACACCCCATGCTTATGGTCTCGAGAGCTCTGTCAATTGATGTTGCTCCAATTACTAATGCAGATGTAAAAACAGCGGGGCCACTTGTTGAGTATGAACGAAGAATTGCTGCAGGGGAACTTGTTGACGGTGATGCCTACCAGGTAGGCACCTTAAGAGAACTTCAAAGGCTTTATGATGAACTTGTTCAATCAGCTGATGCTTGCCAGTTGGATAGATATTCAATTTCTACAAAATCTGGAAGGAATAGGTGGTTGTGGTATCATTTCATCCCACATTCTTCAACCTCACCTGTCAAGGGTCTTTATCTGTATGGAGGAGTGGGTACCGGTAAAACCATGTTGATGGACTTGTTTTTTGATCAGCTACAATGCTGTTGGAGGAAAAAGAGGATTCATTTTCATGACTTTATGCTGAATGTACATAAAACATTGCGAAAGCACCAGGGTGTAGAAGATCCACTTGAAGTTGTTGCAAGAGAGATATCGGACGAGGCAGTCTTGTTGTGTCTGGATGAATTCATGGTGACAGATGTTGCTGATGCATTAATATTAAATCGCCTGTTTAGACATCTATTCAATAATGGTATTATCCTTGTTTCCACCTCCAATCGTGCTCCAGATAAGCTGTATGAAGGTGGACTGCCGATCGATCTTTTTCTGCCGTTCATTGCGACTTTGAAGGAAAGATGTGTAGTTCATGAAATTGGTTCTGCGGTAGACTACCGGAAACTGACATCGGCAGAGCAGGGTTTCTACTTTGTTGGCAAAGATTTGTCGGGATTTCTCAAGCAAAGTTTTCAACAATTGATTGGGGAACACGAAGCTGTTCCACAAGAAGCAGAAGTAGTAATGGGAAGGACATTAAAGGTTCCACTCGGTGCTAATGGATGTGCGTATTTTCCTTTTGAGGAACTCTGTAACAGACCATTGGGAGCTGCAGACTATTTTGGATTGTGCAAGAACTACCATACCTTGGCATTGGAGGGTGTCCCAGTTTTTGGGCTCCACAATAGAACAGCTGCATACCGGTTTGTCACTCTAGTTGATGTGATGTACGAGAACAAGGCCAGGTTGTTGTGTACAGCTGAGGGAACTCCCTTTGAACTCTTTGAAAAGGTAGTGACAATCTCTGATGCCCTACAAATCGCACCTAGAACCTTTTCAAGGTCAAGGAAAAATGATGATGCCGGACTCTGCGTGGATAATGAATTGGGTTTTGCCAAAGACCGCACCATTAGTAGATTAACAGAGATGAATAGCAAAGAATACTTGGAGCACCATGCTGAAACATTGGCTGAGACGAACTCAGAGGAAGGTGTGAACCTTAATAAGGTCGTGCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

503

Amino Acids

56.67

Weight (kDa)

6.39

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 81 - 426 3.7e-87 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 656
Acc65I GGTACC 1 cut(s) 485
AccB1I GGYRCC 2 cut(s) 308, 485
AccB7I CCANNNNNTGG 2 cut(s) 1135, 1160
AccI GTMKAC 1 cut(s) 879
AciI CCGC 3 cut(s) 236, 875, 1390
AclWI GGATC 1 cut(s) 611
AcsI RAATTY 2 cut(s) 146, 677
AdeI CACNNNGTG 1 cut(s) 1040
AfaI GTAC 4 cut(s) 487, 582, 1217, 1243
AfiI CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1326, 1492
AflII CTTAAG 1 cut(s) 313
AgeI ACCGGT 2 cut(s) 488, 1188
AjnI CCWGG 3 cut(s) 300, 603, 1229
AleI CACNNNNGTG 1 cut(s) 606
AluBI AGCT 9 cut(s) 94, 188, 356, 526, 787, 986, 1097, 1181, 1247
AluI AGCT 9 cut(s) 94, 188, 356, 526, 787, 986, 1097, 1181, 1247
Alw21I GWGCWC 3 cut(s) 190, 778, 1440
Alw26I GTCTC 2 cut(s) 184, 1454
AlwI GGATC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 181
AoxI GGCC 2 cut(s) 239, 1227
ApeKI GCWGC 3 cut(s) 269, 1097, 1181
ApoI RAATTY 2 cut(s) 146, 677
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
AseI ATTAAT 1 cut(s) 707
AsiGI ACCGGT 2 cut(s) 488, 1188
Asp700I GAANNNNTTC 3 cut(s) 345, 987, 1321
Asp718I GGTACC 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 4 cut(s) 17, 302, 441, 697
AvaI CYCGRG 1 cut(s) 181
BanI GGYRCC 2 cut(s) 308, 485
BanII GRGCYC 2 cut(s) 190, 1167
Bbv12I GWGCWC 3 cut(s) 190, 778, 1440
BbvCI CCTCAGC 1 cut(s) 1248
BbvI GCAGC 3 cut(s) 256, 1084, 1168
BccI CCATC 2 cut(s) 57, 499
BceAI ACGGC 1 cut(s) 808
BciT130I CCWGG 3 cut(s) 302, 605, 1231
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 2 cut(s) 184, 1454
BfaI CTAG 2 cut(s) 1202, 1314
BfmI CTRYAG 2 cut(s) 270, 1098
BfrI CTTAAG 1 cut(s) 313
BisI GCNGC 3 cut(s) 270, 1098, 1182
BlsI GCNGC 3 cut(s) 271, 1099, 1183
Bme1390I CCNGG 3 cut(s) 302, 605, 1231
BmeT110I CYCGRG 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 5 cut(s) 240, 310, 487, 1032, 1166
BmrFI CCNGG 3 cut(s) 302, 605, 1231
BmrI ACTGGG 1 cut(s) 1148
BmsI GCATC 5 cut(s) 283, 349, 691, 1285, 1336
BmuI ACTGGG 1 cut(s) 1148
BplI GAGNNNNNCTC 2 cut(s) 1451, 1483
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 1248
BpuEI CTTGAG 2 cut(s) 52, 932
Bsa29I ATCGAT 1 cut(s) 810
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 2 cut(s) 604, 1134
BsaWI WCCGGW 3 cut(s) 488, 885, 1188
Bsc4I CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1326, 1492
Bse118I RCCGGY 2 cut(s) 488, 1188
Bse1I ACTGG 2 cut(s) 367, 1154
Bse3DI GCAATG 2 cut(s) 590, 828
BseBI CCWGG 3 cut(s) 302, 605, 1231
BseCI ATCGAT 1 cut(s) 810
BseDI CCNNGG 2 cut(s) 604, 1134
BseGI GGATG 3 cut(s) 429, 679, 1055
BseLI CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1326, 1492
BseMI GCAATG 2 cut(s) 590, 828
BseMII CTCAG 3 cut(s) 1239, 1449, 1482
BseNI ACTGG 2 cut(s) 367, 1154
BseRI GAGGAG 1 cut(s) 492
BseXI GCAGC 3 cut(s) 256, 1084, 1168
Bsh1285I CGRYCG 1 cut(s) 810
BshFI GGCC 2 cut(s) 241, 1229
BshNI GGYRCC 2 cut(s) 308, 485
BshTI ACCGGT 2 cut(s) 488, 1188
BshVI ATCGAT 1 cut(s) 810
BsiEI CGRYCG 1 cut(s) 810
BsiHKAI GWGCWC 3 cut(s) 190, 778, 1440
BsiHKCI CYCGRG 1 cut(s) 181
BsiSI CCGG 4 cut(s) 489, 886, 1189, 1350
BslFI GGGAC 1 cut(s) 1136
BslI CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1326, 1492
BsmAI GTCTC 2 cut(s) 184, 1454
BsmBI CGTCTC 1 cut(s) 1454
BsmFI GGGAC 1 cut(s) 1136
BsnI GGCC 2 cut(s) 241, 1229
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 181
Bsp1286I GDGCHC 4 cut(s) 190, 778, 1167, 1440
Bsp1407I TGTACA 2 cut(s) 580, 1241
Bsp143I GATC 5 cut(s) 17, 520, 616, 807, 811
BspACI CCGC 3 cut(s) 236, 875, 1390
BspANI GGCC 2 cut(s) 241, 1229
BspCNI CTCAG 3 cut(s) 1240, 1450, 1481
BspDI ATCGAT 1 cut(s) 810
BspHI TCATGA 2 cut(s) 562, 859
BspLI GGNNCC 5 cut(s) 240, 310, 487, 1032, 1166
BspMAI CTGCAG 2 cut(s) 274, 1102
BspPI GGATC 1 cut(s) 611
BspT107I GGYRCC 2 cut(s) 308, 485
BspTI CTTAAG 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 590, 828
BsrFI RCCGGY 2 cut(s) 488, 1188
BsrGI TGTACA 2 cut(s) 580, 1241
BsrI ACTGG 2 cut(s) 367, 1154
BssAI RCCGGY 2 cut(s) 488, 1188
BssECI CCNNGG 2 cut(s) 604, 1134
BssMI GATC 5 cut(s) 17, 520, 616, 807, 811
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 3 cut(s) 302, 605, 1231
Bst4CI ACNGT 1 cut(s) 290
BstAFI CTTAAG 1 cut(s) 313
BstAUI TGTACA 2 cut(s) 580, 1241
BstC8I GCNNGC 2 cut(s) 364, 1503
BstDEI CTNAG 3 cut(s) 1248, 1458, 1468
BstENI CCTNNNNNAGG 2 cut(s) 1324, 1490
BstF5I GGATG 3 cut(s) 429, 679, 1055
BstKTI GATC 5 cut(s) 20, 523, 619, 810, 814
BstMAI GTCTC 2 cut(s) 184, 1454
BstMBI GATC 5 cut(s) 17, 520, 616, 807, 811
BstMCI CGRYCG 1 cut(s) 810
BstMWI GCNNNNNNNGC 2 cut(s) 119, 140
BstNI CCWGG 3 cut(s) 302, 605, 1231
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 3 cut(s) 300, 603, 1229
BstSFI CTRYAG 2 cut(s) 270, 1098
BstV1I GCAGC 3 cut(s) 256, 1084, 1168
BstX2I RGATCY 1 cut(s) 616
BstXI CCANNNNNNTGG 2 cut(s) 175, 505
BstYI RGATCY 1 cut(s) 616
Bsu15I ATCGAT 1 cut(s) 810
BsuRI GGCC 2 cut(s) 241, 1229
BsuTUI ATCGAT 1 cut(s) 810
BtsCI GGATG 3 cut(s) 429, 679, 1055
Cac8I GCNNGC 2 cut(s) 364, 1503
CaiI CAGNNNCTG 1 cut(s) 94
CciI TCATGA 2 cut(s) 562, 859
Cfr10I RCCGGY 2 cut(s) 488, 1188
Cfr13I GGNCC 1 cut(s) 239
ClaI ATCGAT 1 cut(s) 810
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 4 cut(s) 486, 581, 1216, 1242
CspAI ACCGGT 2 cut(s) 488, 1188
CviAII CATG 8 cut(s) 57, 169, 499, 563, 682, 860, 1442, 1506
CviQI GTAC 4 cut(s) 486, 581, 1216, 1242
DdeI CTNAG 3 cut(s) 1248, 1458, 1468
DpnI GATC 5 cut(s) 19, 522, 618, 809, 813
DpnII GATC 5 cut(s) 17, 520, 616, 807, 811
DraIII CACNNNGTG 1 cut(s) 1040
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
Ecl136II GAGCTC 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 1134
Eco24I GRGCYC 2 cut(s) 190, 1167
Eco31I GGTCTC 1 cut(s) 184
Eco32I GATATC 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 188
EcoNI CCTNNNNNAGG 2 cut(s) 1324, 1490
EcoRI GAATTC 2 cut(s) 146, 677
EcoRII CCWGG 3 cut(s) 300, 603, 1229
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 2 cut(s) 190, 1167
ErhI CCWWGG 1 cut(s) 1134
Esp3I CGTCTC 1 cut(s) 1454
FaeI CATG 8 cut(s) 60, 172, 502, 566, 685, 863, 1445, 1509
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 1 cut(s) 1136
FatI CATG 8 cut(s) 56, 168, 498, 562, 681, 859, 1441, 1505
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 1 cut(s) 879
Fnu4HI GCNGC 3 cut(s) 270, 1098, 1182
FokI GGATG 3 cut(s) 416, 686, 1062
FriOI GRGCYC 2 cut(s) 190, 1167
Fsp4HI GCNGC 3 cut(s) 270, 1098, 1182
FspBI CTAG 2 cut(s) 1202, 1314
GluI GCNGC 3 cut(s) 270, 1098, 1182
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 2 cut(s) 241, 1229
HapII CCGG 4 cut(s) 489, 886, 1189, 1350
Hin1II CATG 8 cut(s) 60, 172, 502, 566, 685, 863, 1445, 1509
HincII GTYRAC 2 cut(s) 99, 286
HindII GTYRAC 2 cut(s) 99, 286
HinfI GANTC 2 cut(s) 553, 1353
HpaII CCGG 4 cut(s) 489, 886, 1189, 1350
HphI GGTGA 4 cut(s) 17, 302, 441, 697
Hpy166II GTNNAC 6 cut(s) 99, 286, 800, 880, 1242, 1483
Hpy188I TCNGA 3 cut(s) 649, 1294, 1471
Hpy188III TCNNGA 8 cut(s) 181, 183, 399, 563, 671, 779, 860, 939
Hpy8I GTNNAC 6 cut(s) 99, 286, 800, 880, 1242, 1483
HpyAV CCTTC 5 cut(s) 396, 788, 835, 1011, 1469
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 8 cut(s) 221, 272, 635, 704, 1100, 1119, 1184, 1501
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 140
HpyF3I CTNAG 3 cut(s) 1248, 1458, 1468
Hsp92II CATG 8 cut(s) 60, 172, 502, 566, 685, 863, 1445, 1509
KpnI GGTACC 1 cut(s) 489
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 5 cut(s) 17, 520, 616, 807, 811
LmnI GCTCC 5 cut(s) 211, 781, 1094, 1170, 1435
Lsp1109I GCAGC 3 cut(s) 256, 1084, 1168
LweI GCATC 5 cut(s) 283, 349, 691, 1285, 1336
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 2 cut(s) 1202, 1314
MaeIII GTNAC 5 cut(s) 79, 685, 1079, 1195, 1282
MalI GATC 5 cut(s) 19, 522, 618, 809, 813
MboI GATC 5 cut(s) 17, 520, 616, 807, 811
MboII GAAGA 3 cut(s) 273, 432, 626
MfeI CAATTG 3 cut(s) 9, 195, 965
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 4 cut(s) 190, 778, 1167, 1440
MlyI GAGTC 1 cut(s) 1347
MmeI TCCRAC 2 cut(s) 351, 518
Mph1103I ATGCAT 1 cut(s) 706
MroXI GAANNNNTTC 3 cut(s) 345, 987, 1321
MseI TTAA 6 cut(s) 314, 707, 713, 1025, 1406, 1488
MslI CAYNNNNRTG 4 cut(s) 173, 503, 561, 606
MspA1I CMGCKG 4 cut(s) 236, 356, 1181, 1247
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 4 cut(s) 489, 886, 1189, 1350
MspR9I CCNGG 3 cut(s) 302, 605, 1231
MunI CAATTG 3 cut(s) 9, 195, 965
MvaI CCWGG 3 cut(s) 302, 605, 1231
MwoI GCNNNNNNNGC 2 cut(s) 119, 140
NdeII GATC 5 cut(s) 17, 520, 616, 807, 811
NlaIII CATG 8 cut(s) 60, 172, 502, 566, 685, 863, 1445, 1509
NlaIV GGNNCC 5 cut(s) 240, 310, 487, 1032, 1166
NmuCI GTSAC 3 cut(s) 685, 1195, 1282
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 606
PaeR7I CTCGAG 1 cut(s) 181
PagI TCATGA 2 cut(s) 562, 859
PdmI GAANNNNTTC 3 cut(s) 345, 987, 1321
PfeI GAWTC 1 cut(s) 553
PflMI CCANNNNNTGG 2 cut(s) 1135, 1160
PinAI ACCGGT 2 cut(s) 488, 1188
PkrI GCNGC 3 cut(s) 271, 1099, 1183
Ple19I CGATCG 1 cut(s) 810
PleI GAGTC 1 cut(s) 1347
PpsI GAGTC 1 cut(s) 1347
PshBI ATTAAT 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 3 cut(s) 300, 603, 1229
PspGI CCWGG 3 cut(s) 300, 603, 1229
PspN4I GGNNCC 5 cut(s) 240, 310, 487, 1032, 1166
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 2 cut(s) 274, 1102
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 616
PvuI CGATCG 1 cut(s) 810
PvuII CAGCTG 3 cut(s) 356, 1181, 1247
RsaI GTAC 4 cut(s) 487, 582, 1217, 1243
RsaNI GTAC 4 cut(s) 486, 581, 1216, 1242
RseI CAYNNNNRTG 4 cut(s) 173, 503, 561, 606
SacI GAGCTC 1 cut(s) 190
SaqAI TTAA 6 cut(s) 314, 707, 713, 1025, 1406, 1488
SatI GCNGC 3 cut(s) 270, 1098, 1182
Sau3AI GATC 5 cut(s) 17, 520, 616, 807, 811
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 3 cut(s) 302, 605, 1231
SduI GDGCHC 4 cut(s) 190, 778, 1167, 1440
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 5 cut(s) 283, 349, 691, 1285, 1336
SfcI CTRYAG 2 cut(s) 270, 1098
Sfr274I CTCGAG 1 cut(s) 181
SlaI CTCGAG 1 cut(s) 181
SmiMI CAYNNNNRTG 4 cut(s) 173, 503, 561, 606
SmlI CTYRAG 4 cut(s) 67, 181, 313, 947
SmoI CTYRAG 4 cut(s) 67, 181, 313, 947
SsiI CCGC 3 cut(s) 236, 875, 1390
SspI AATATT 1 cut(s) 711
SspMI CTAG 2 cut(s) 1202, 1314
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 3 cut(s) 300, 603, 1229
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 4 cut(s) 16, 150, 182, 810
TatI WGTACW 2 cut(s) 580, 1241
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 6 cut(s) 314, 707, 713, 1025, 1406, 1488
Tru9I TTAA 6 cut(s) 314, 707, 713, 1025, 1406, 1488
TseFI GTSAC 3 cut(s) 685, 1195, 1282
TseI GCWGC 3 cut(s) 269, 1097, 1181
Tsp45I GTSAC 3 cut(s) 685, 1195, 1282
Van91I CCANNNNNTGG 2 cut(s) 1135, 1160
Vha464I CTTAAG 1 cut(s) 313
VspI ATTAAT 1 cut(s) 707
XagI CCTNNNNNAGG 2 cut(s) 1324, 1490
XapI RAATTY 2 cut(s) 146, 677
XceI RCATGY 1 cut(s) 60
XhoI CTCGAG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 879
XmnI GAANNNNTTC 3 cut(s) 345, 987, 1321
XspI CTAG 2 cut(s) 1202, 1314
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.