pycom17g04640

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002869
Physical Location & Seq
Reverse (-)
3201697 .. 3202394
698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
ATGGACCTTTCTTTTCAGGTCCCAATTTATGCAGTAGCTCAAATAGCAGGATCAATATCTGCCTCGTTTACCCTGTATGTACTGTTGCACCCAATTAAACAACTTGGCACCGCATCACCTTCTGCACCAGAGACCCAAGCTTTGATTGCAGAGATAATCATGACATTTTCCATGGTGTTCATAGCCTCCGCCGTCGCAACTGATACCAAAGTAGGAGAACTAGCAGGCATCGCAGTTGGCTCCGCGGTTTGCATAACTTCAATCTTTGCCGGACTAGACAAACATGTCGTGCATATATTTAAGCAAGTGAAACAGCTAACATGGGTCTACGTCGTTGGACCAGTGATAGGAGCCCTACTTGGCTCATGGTCCTACAGCTTCATTCGGGTCAGCGATAACCCCATCCAGGCAGTATCACCACCCTCATTCGCATTCAAGCTTCGTCGAATCAAGACCGACAGCAACAGACAACTTACCTTCAGAGACCCTCTGGATTCAGCTTGA

Protein Analysis

168

Amino Acids

17.94

Weight (kDa)

8.79

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 5 - 125 6.7e-13 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 327
AccII CGCG 1 cut(s) 245
AciI CCGC 4 cut(s) 111, 189, 243, 245
AclWI GGATC 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 463
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 347, 406
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 2 cut(s) 261, 436
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 6 cut(s) 38, 140, 316, 378, 439, 500
AluI AGCT 6 cut(s) 38, 140, 316, 378, 439, 500
Alw26I GTCTC 2 cut(s) 125, 477
AlwI GGATC 1 cut(s) 58
ArsI GACNNNNNNTTYG 2 cut(s) 124, 156
AspS9I GGNCC 4 cut(s) 4, 19, 338, 369
AsuHPI GGTGA 2 cut(s) 108, 408
AvaII GGWCC 4 cut(s) 4, 19, 338, 369
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BanI GGYRCC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 355
BccI CCATC 1 cut(s) 410
BceAI ACGGC 1 cut(s) 176
BciT130I CCWGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 125, 477
BfaI CTAG 2 cut(s) 221, 275
BfmI CTRYAG 1 cut(s) 373
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 4 cut(s) 4, 19, 338, 369
BmgT120I GGNCC 4 cut(s) 4, 19, 338, 369
BmiI GGNNCC 4 cut(s) 21, 109, 241, 352
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 2 cut(s) 122, 237
BsaBI GATNNNNATC 1 cut(s) 55
BsaI GGTCTC 2 cut(s) 125, 477
BsaJI CCNNGG 2 cut(s) 171, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 406
Bse1I ACTGG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 55
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 171, 243
BseGI GGATG 1 cut(s) 402
BseJI GATNNNNATC 1 cut(s) 55
BseLI CCNNNNNNNGG 2 cut(s) 347, 406
BseNI ACTGG 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 245
BshNI GGYRCC 1 cut(s) 107
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 5
BslI CCNNNNNNNGG 2 cut(s) 347, 406
BsmAI GTCTC 2 cut(s) 125, 477
BsmFI GGGAC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 431
Bso31I GGTCTC 2 cut(s) 125, 477
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 1 cut(s) 50
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 4 cut(s) 111, 189, 243, 245
BspFNI CGCG 1 cut(s) 245
BspHI TCATGA 1 cut(s) 159
BspLI GGNNCC 4 cut(s) 21, 109, 241, 352
BspPI GGATC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 2 cut(s) 125, 477
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 171, 243
BssMI GATC 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 226
BstDSI CCRYGG 2 cut(s) 171, 243
BstF5I GGATG 1 cut(s) 402
BstFNI CGCG 1 cut(s) 245
BstKTI GATC 1 cut(s) 53
BstMAI GTCTC 2 cut(s) 125, 477
BstMBI GATC 1 cut(s) 50
BstMWI GCNNNNNNNGC 3 cut(s) 44, 146, 230
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 373
BstUI CGCG 1 cut(s) 245
BtgI CCRYGG 2 cut(s) 171, 243
BtgZI GCGATG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 226
CciI TCATGA 1 cut(s) 159
Cfr13I GGNCC 4 cut(s) 4, 19, 338, 369
Cfr42I CCGCGG 1 cut(s) 246
Csp6I GTAC 1 cut(s) 80
CviAII CATG 5 cut(s) 160, 172, 284, 321, 366
CviQI GTAC 1 cut(s) 80
DpnI GATC 1 cut(s) 52
DpnII GATC 1 cut(s) 50
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EciI GGCGGA 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 2 cut(s) 125, 477
Eco47I GGWCC 4 cut(s) 4, 19, 338, 369
Eco57I CTGAAG 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 5 cut(s) 163, 175, 287, 324, 369
FaqI GGGAC 1 cut(s) 5
FatI CATG 5 cut(s) 159, 171, 283, 320, 365
FblI GTMKAC 1 cut(s) 327
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 355
FspBI CTAG 2 cut(s) 221, 275
HapII CCGG 1 cut(s) 270
Hin1II CATG 5 cut(s) 163, 175, 287, 324, 369
HindIII AAGCTT 2 cut(s) 138, 437
HinfI GANTC 2 cut(s) 447, 494
HpaII CCGG 1 cut(s) 270
HphI GGTGA 2 cut(s) 108, 408
Hpy166II GTNNAC 2 cut(s) 69, 328
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 3 cut(s) 160, 451, 491
Hpy8I GTNNAC 2 cut(s) 69, 328
Hpy99I CGWCG 3 cut(s) 197, 335, 447
HpyAV CCTTC 2 cut(s) 129, 487
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 6 cut(s) 32, 88, 125, 149, 252, 292
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 146, 230
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 5 cut(s) 163, 175, 287, 324, 369
KspI CCGCGG 1 cut(s) 246
Kzo9I GATC 1 cut(s) 50
LmnI GCTCC 2 cut(s) 245, 350
LweI GCATC 2 cut(s) 122, 237
MaeI CTAG 2 cut(s) 221, 275
MaeII ACGT 1 cut(s) 330
MalI GATC 1 cut(s) 52
MboI GATC 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 2 cut(s) 24, 93
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 4 cut(s) 73, 196, 433, 498
MseI TTAA 2 cut(s) 96, 300
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 407
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 44, 146, 230
NcoI CCATGG 1 cut(s) 171
NdeII GATC 1 cut(s) 50
NlaIII CATG 5 cut(s) 163, 175, 287, 324, 369
NlaIV GGNNCC 4 cut(s) 21, 109, 241, 352
NspI RCATGY 1 cut(s) 287
PagI TCATGA 1 cut(s) 159
PciI ACATGT 1 cut(s) 283
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 447, 494
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 4 cut(s) 21, 109, 241, 352
PspPI GGNCC 4 cut(s) 4, 19, 338, 369
PspPPI RGGWCCY 1 cut(s) 19
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SacII CCGCGG 1 cut(s) 246
SaqAI TTAA 2 cut(s) 96, 300
Sau3AI GATC 1 cut(s) 50
Sau96I GGNCC 4 cut(s) 4, 19, 338, 369
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 355
SfaNI GCATC 2 cut(s) 122, 237
SfcI CTRYAG 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 246
SgrBI CCGCGG 1 cut(s) 246
SinI GGWCC 4 cut(s) 4, 19, 338, 369
Sse9I AATT 2 cut(s) 24, 93
SsiI CCGC 4 cut(s) 111, 189, 243, 245
SspMI CTAG 2 cut(s) 221, 275
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 84
TaiI ACGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 445
TaqII GACCGA 1 cut(s) 470
TasI AATT 2 cut(s) 24, 93
TatI WGTACW 1 cut(s) 79
TfiI GAWTC 2 cut(s) 447, 494
Tru1I TTAA 2 cut(s) 96, 300
Tru9I TTAA 2 cut(s) 96, 300
TscAI CASTG 1 cut(s) 348
TspDTI ATGAA 2 cut(s) 169, 370
TspRI CASTG 1 cut(s) 348
VpaK11BI GGWCC 4 cut(s) 4, 19, 338, 369
XceI RCATGY 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 2 cut(s) 221, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.