pycom17g15160

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002322
Physical Location & Seq
Forward (+)
12970358 .. 12972709
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 966 bp
ATGGTCAAAATCGCGGATTCGAGCAGATTTCACGGCCTATGCATACGGTCGTCGGAGGAATTTTGGCGCGAAAATATCCATGGCGGTTTCGCCACTCTCGTCGCCGTGCTCTTCGTCTTTGCTTGCCACTGTGCCAAGAGGTTCTTCTTTGGAAAACCCTCTGGCTCCAGTGCTTCTCCGGTCGGTCTATCGGATTCTGATCTCCCCACTTCTGGGTCTCGGATTCCGCCGCACAGCACCTTGGAACTGGTGACAGATGCAGATTTGAAGTTTCTGATTGATAACTTGGGGGAGAAAACGGGCGAAAATGAGAAATGGGAAAAGGTCATAGACAAAAGAAATGATATTCTATCCTACTATGCGAAGTGCTGCAAGCCTAAGGATAGTCCTTTGACATACTTGAGCGTGACGATATTTGAGAATTGCTCTCCTGAGAAGCTGAGAGACTTCTACATGGACAATGATTACAGGAAGCAATGGGATAAGATGCTGATTCAGCATGAGCAGTTACAGATGGACAAAAACAAGGGGGTTGAAGTTGGTCGGACAATAAAAAAGTTTCCACTGTTGACAGCCAGAGAATATGTATTAGCTTGGAGATTGTGGGAGGGGAAAGATAACACCTTTTATTGTTTTATCAAGGAATGTGAACATCCTTTGGCACCACGCCAGAAGAAGTATGTGCGTGTTAGTACTTTCAGATCTGGTTGGCGAATCCGAAAAGTGCCTGATACTAATGCCTGTGAGATCAAGATGTTTCATCAAGAAGATGCCGGTTTAAATGTGGAGATGGCAAAATTGGCTTTTTCTAGGGGCATATGGAGCTATGTGTGCAAGATGGGCAATGCACTTAACAGATATACTACAATCAGCTCTCATCAATCAAGGGCTGCTGCTTCTGCTGTCACGTTGATTAAAAAGGCCAGGACAACTTTCTTTGATGGATTAGTATTGTTTAAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

322

Amino Acids

36.9

Weight (kDa)

9.17

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 100 - 255 7.2e-09 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 661
AccII CGCG 2 cut(s) 14, 69
AciI CCGC 4 cut(s) 14, 84, 227, 230
AcsI RAATTY 2 cut(s) 59, 959
AfaI GTAC 1 cut(s) 694
AfiI CCNNNNNNNGG 2 cut(s) 212, 213
AgsI TTSAA 2 cut(s) 268, 536
AjnI CCWGG 1 cut(s) 923
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 4 cut(s) 439, 593, 825, 873
AluI AGCT 4 cut(s) 439, 593, 825, 873
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 2 cut(s) 222, 438
AoxI GGCC 2 cut(s) 34, 921
ApeKI GCWGC 3 cut(s) 369, 890, 893
ApoI RAATTY 2 cut(s) 59, 959
AspLEI GCGC 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 262
AxyI CCTNAGG 1 cut(s) 378
BanI GGYRCC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 356, 877, 880
BccI CCATC 4 cut(s) 508, 784, 832, 935
BceAI ACGGC 2 cut(s) 49, 89
BciT130I CCWGG 1 cut(s) 925
BcoDI GTCTC 2 cut(s) 222, 438
BfaI CTAG 1 cut(s) 810
BglII AGATCT 1 cut(s) 701
BisI GCNGC 4 cut(s) 230, 370, 891, 894
BlsI GCNGC 4 cut(s) 231, 371, 892, 895
BmcAI AGTACT 1 cut(s) 694
Bme1390I CCNGG 1 cut(s) 925
BmiI GGNNCC 2 cut(s) 166, 663
BmrFI CCNGG 1 cut(s) 925
BmsI GCATC 3 cut(s) 247, 477, 760
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 421
BsaBI GATNNNNATC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 79, 240
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 213
Bse118I RCCGGY 1 cut(s) 773
Bse1I ACTGG 2 cut(s) 168, 252
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 2 cut(s) 482, 850
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 1 cut(s) 925
BseDI CCNNGG 2 cut(s) 79, 240
BseGI GGATG 1 cut(s) 652
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 212, 213
BseMI GCAATG 2 cut(s) 482, 850
BseMII CTCAG 2 cut(s) 423, 431
BseNI ACTGG 2 cut(s) 168, 252
BseXI GCAGC 3 cut(s) 356, 877, 880
Bsh1236I CGCG 2 cut(s) 14, 69
Bsh1285I CGRYCG 2 cut(s) 50, 183
BshFI GGCC 2 cut(s) 36, 923
BshNI GGYRCC 1 cut(s) 661
BsiEI CGRYCG 2 cut(s) 50, 183
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 2 cut(s) 179, 774
BslI CCNNNNNNNGG 2 cut(s) 212, 213
BsmAI GTCTC 2 cut(s) 222, 438
BsnI GGCC 2 cut(s) 36, 923
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 3 cut(s) 199, 701, 747
Bsp19I CCATGG 1 cut(s) 79
BspACI CCGC 4 cut(s) 14, 84, 227, 230
BspANI GGCC 2 cut(s) 36, 923
BspCNI CTCAG 2 cut(s) 424, 432
BspFNI CGCG 2 cut(s) 14, 69
BspLI GGNNCC 2 cut(s) 166, 663
BspQI GCTCTTC 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 661
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 2 cut(s) 482, 850
BsrFI RCCGGY 1 cut(s) 773
BsrI ACTGG 2 cut(s) 168, 252
BssAI RCCGGY 1 cut(s) 773
BssECI CCNNGG 2 cut(s) 79, 240
BssMI GATC 3 cut(s) 199, 701, 747
BssT1I CCWWGG 2 cut(s) 79, 240
Bst2UI CCWGG 1 cut(s) 925
Bst4CI ACNGT 3 cut(s) 48, 131, 567
Bst6I CTCTTC 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 124, 374
BstDEI CTNAG 3 cut(s) 378, 432, 440
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 1 cut(s) 652
BstFNI CGCG 2 cut(s) 14, 69
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 3 cut(s) 202, 704, 750
BstMAI GTCTC 2 cut(s) 222, 438
BstMBI GATC 3 cut(s) 199, 701, 747
BstMCI CGRYCG 2 cut(s) 50, 183
BstMWI GCNNNNNNNGC 6 cut(s) 496, 800, 822, 831, 840, 899
BstNI CCWGG 1 cut(s) 925
BstSCI CCNGG 1 cut(s) 923
BstUI CGCG 2 cut(s) 14, 69
BstV1I GCAGC 3 cut(s) 356, 877, 880
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 2 cut(s) 36, 923
BtgI CCRYGG 1 cut(s) 79
BtsCI GGATG 1 cut(s) 652
BtsIMutI CAGTG 3 cut(s) 127, 175, 563
Cac8I GCNNGC 2 cut(s) 124, 374
CfoI GCGC 1 cut(s) 69
Cfr10I RCCGGY 1 cut(s) 773
Csp6I GTAC 1 cut(s) 693
CviAII CATG 3 cut(s) 80, 454, 500
CviQI GTAC 1 cut(s) 693
DdeI CTNAG 3 cut(s) 378, 432, 440
DpnI GATC 3 cut(s) 201, 703, 749
DpnII GATC 3 cut(s) 199, 701, 747
DraI TTTAAA 2 cut(s) 780, 958
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 79, 240
Eco31I GGTCTC 1 cut(s) 222
Eco81I CCTNAGG 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 923
EcoT14I CCWWGG 2 cut(s) 79, 240
EcoT22I ATGCAT 1 cut(s) 44
ErhI CCWWGG 2 cut(s) 79, 240
FaeI CATG 3 cut(s) 83, 457, 503
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FatI CATG 3 cut(s) 79, 453, 499
FauNDI CATATG 1 cut(s) 818
Fnu4HI GCNGC 4 cut(s) 230, 370, 891, 894
FokI GGATG 1 cut(s) 639
Fsp4HI GCNGC 4 cut(s) 230, 370, 891, 894
FspBI CTAG 1 cut(s) 810
GlaI GCGC 1 cut(s) 68
GluI GCNGC 4 cut(s) 230, 370, 891, 894
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 2 cut(s) 36, 923
HapII CCGG 2 cut(s) 179, 774
HhaI GCGC 1 cut(s) 69
Hin1II CATG 3 cut(s) 83, 457, 503
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HinfI GANTC 5 cut(s) 17, 194, 223, 493, 714
HpaII CCGG 2 cut(s) 179, 774
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 2 cut(s) 570, 650
Hpy188I TCNGA 8 cut(s) 55, 193, 199, 222, 276, 546, 701, 719
Hpy188III TCNNGA 3 cut(s) 431, 751, 764
Hpy8I GTNNAC 2 cut(s) 570, 650
Hpy99I CGWCG 2 cut(s) 55, 104
HpyCH4III ACNGT 3 cut(s) 48, 131, 567
HpyCH4IV ACGT 1 cut(s) 908
HpyCH4V TGCA 5 cut(s) 42, 260, 372, 834, 848
HpyF10VI GCNNNNNNNGC 6 cut(s) 496, 800, 822, 831, 840, 899
HpyF3I CTNAG 3 cut(s) 378, 432, 440
HpySE526I ACGT 1 cut(s) 908
Hsp92II CATG 3 cut(s) 83, 457, 503
HspAI GCGC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 199, 701, 747
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 170, 822
Lsp1109I GCAGC 3 cut(s) 356, 877, 880
LweI GCATC 3 cut(s) 247, 477, 760
MaeI CTAG 1 cut(s) 810
MaeII ACGT 1 cut(s) 908
MaeIII GTNAC 4 cut(s) 250, 406, 507, 904
MalI GATC 3 cut(s) 201, 703, 749
MboI GATC 3 cut(s) 199, 701, 747
MboII GAAGA 4 cut(s) 103, 136, 685, 779
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 4 cut(s) 59, 421, 797, 959
MmeI TCCRAC 2 cut(s) 33, 524
MnlI CCTC 4 cut(s) 49, 132, 169, 601
Mph1103I ATGCAT 1 cut(s) 44
MseI TTAA 5 cut(s) 779, 852, 915, 957, 964
MspI CCGG 2 cut(s) 179, 774
MspR9I CCNGG 1 cut(s) 925
MvaI CCWGG 1 cut(s) 925
MvnI CGCG 2 cut(s) 14, 69
MwoI GCNNNNNNNGC 6 cut(s) 496, 800, 822, 831, 840, 899
NcoI CCATGG 1 cut(s) 79
NdeI CATATG 1 cut(s) 818
NdeII GATC 3 cut(s) 199, 701, 747
NlaIII CATG 3 cut(s) 83, 457, 503
NlaIV GGNNCC 2 cut(s) 166, 663
NmuCI GTSAC 3 cut(s) 250, 406, 904
NsiI ATGCAT 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 5 cut(s) 17, 194, 223, 493, 714
PkrI GCNGC 4 cut(s) 231, 371, 892, 895
Psp6I CCWGG 1 cut(s) 923
PspGI CCWGG 1 cut(s) 923
PspN4I GGNNCC 2 cut(s) 166, 663
PsuI RGATCY 1 cut(s) 701
RsaI GTAC 1 cut(s) 694
RsaNI GTAC 1 cut(s) 693
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 5 cut(s) 779, 852, 915, 957, 964
SatI GCNGC 4 cut(s) 230, 370, 891, 894
Sau3AI GATC 3 cut(s) 199, 701, 747
ScaI AGTACT 1 cut(s) 694
ScrFI CCNGG 1 cut(s) 925
SduI GDGCHC 1 cut(s) 111
SetI ASST 9 cut(s) 143, 242, 327, 441, 595, 626, 827, 875, 911
SfaNI GCATC 3 cut(s) 247, 477, 760
SmlI CTYRAG 1 cut(s) 400
SmoI CTYRAG 1 cut(s) 400
Sse9I AATT 4 cut(s) 59, 421, 797, 959
SsiI CCGC 4 cut(s) 14, 84, 227, 230
SspMI CTAG 1 cut(s) 810
StyD4I CCNGG 1 cut(s) 923
StyI CCWWGG 2 cut(s) 79, 240
TaaI ACNGT 3 cut(s) 48, 131, 567
TaiI ACGT 1 cut(s) 911
TaqI TCGA 1 cut(s) 20
TaqII GACCGA 1 cut(s) 173
TasI AATT 4 cut(s) 59, 421, 797, 959
TatI WGTACW 1 cut(s) 692
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 5 cut(s) 17, 194, 223, 493, 714
Tru1I TTAA 5 cut(s) 779, 852, 915, 957, 964
Tru9I TTAA 5 cut(s) 779, 852, 915, 957, 964
TscAI CASTG 3 cut(s) 134, 175, 570
TseFI GTSAC 3 cut(s) 250, 406, 904
TseI GCWGC 3 cut(s) 369, 890, 893
Tsp45I GTSAC 3 cut(s) 250, 406, 904
TspDTI ATGAA 1 cut(s) 749
TspRI CASTG 3 cut(s) 134, 175, 570
XapI RAATTY 2 cut(s) 59, 959
XspI CTAG 1 cut(s) 810
ZrmI AGTACT 1 cut(s) 694
Zsp2I ATGCAT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.