RchiOBHm_Chr1g0314251

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002225
Physical Location & Seq
Reverse (-)
1544760 .. 1549639
4880 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGGCTCTTGTCAGTAGGCTTCAATCTCTGTCTTCCTCTTTGTCTTCCACCCATTTCAGAAAACCCAATTCCTTAAACCCTCAACCCATTTGCTCCCTACACTTCAACAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCTATTGAGAGGTACCAGAGAGACCAGTGGCTATACAATAACCAATTGGAGCAACCCTCTCTTTTCTCTCTCCCTCCTGATACTGATGCTGATTCTGATTTCACAAGGCAGAATGACATTGCTTTGCAGTTACCAGAGCTAAGAAAGCTGCTTCAGGTGCTGCGAGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGAGGAGGCAAGTGTGGACCTGGCAATGTCTTCTTGGTCGGCACTGGCCCTGGGGACCCTGAACTCCTCACATTGAAGGCTGTCAGAGTTGTACAGAGTGCTGATTTATTGTTGTATGATAGGCTGGTGTCCAATCATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGGACCCAGGAGGAGATACATGAACTGCTACTGAGTTTTGCTGAAGCTGGAGCTACTGTTGTGAGACTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTTTGCAACAGCAAGGAATTCGAGTGCAAGTTATTCCAGGTATCACTGCTGCTTCTGGGATAGCAGCAGAGCTAGGGATCCCATTAACTCACAGAGGTGTTGCAAATAGTGTAAGATTTCTGACCGGGCACTCCAGGAAGGGAGGAACCGATCCTCTCTTTGTGGCAGAGAATGCAGCTGACCCTGATTCAACCTTGGTGGTATACATGGGTTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCCACCAACAACACCAGCTGTTGCAGTTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTGAAGGATCTTGCCAATGAAATTACATTAGCAGAGTTAGTATCACCAACGCTCATCGTCATTGGGAAAGTAGTTGCACTTTCACCATTGTGGCCACATACTTTGAACGAAGTGGCTGCTCTTGTTGGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

377

Amino Acids

40.7

Weight (kDa)

6.13

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 125 - 337 2.5e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 159, 540
AccB1I GGYRCC 2 cut(s) 159, 540
AccI GTMKAC 2 cut(s) 870, 884
AclWI GGATC 6 cut(s) 623, 636, 739, 752, 812, 1011
AcoI YGGCCR 1 cut(s) 1088
AcsI RAATTY 1 cut(s) 684
AcuI CTGAAG 3 cut(s) 284, 612, 1019
AfaI GTAC 3 cut(s) 161, 438, 542
AgsI TTSAA 5 cut(s) 23, 106, 421, 858, 1102
AjnI CCWGG 5 cut(s) 364, 394, 555, 703, 800
AleI CACNNNNGTG 2 cut(s) 762, 1084
AluBI AGCT 7 cut(s) 286, 295, 596, 602, 739, 845, 944
AluI AGCT 7 cut(s) 286, 295, 596, 602, 739, 845, 944
Alw26I GTCTC 2 cut(s) 162, 607
AlwI GGATC 6 cut(s) 623, 636, 739, 752, 812, 1011
AlwNI CAGNNNCTG 1 cut(s) 307
AoxI GGCC 2 cut(s) 391, 1088
ApeKI GCWGC 6 cut(s) 295, 307, 716, 731, 842, 1112
ApoI RAATTY 1 cut(s) 684
Asp718I GGTACC 2 cut(s) 159, 540
AspLEI GCGC 1 cut(s) 981
AspS9I GGNCC 6 cut(s) 362, 392, 400, 552, 913, 963
AsuC2I CCSGG 1 cut(s) 793
AsuHPI GGTGA 2 cut(s) 1032, 1071
AvaII GGWCC 5 cut(s) 362, 400, 552, 913, 963
BaeGI GKGCMC 1 cut(s) 798
BalI TGGCCA 1 cut(s) 1090
BamHI GGATCC 2 cut(s) 628, 744
BanI GGYRCC 2 cut(s) 159, 540
BbsI GAAGAC 3 cut(s) 24, 36, 367
BbvI GCAGC 6 cut(s) 282, 294, 703, 743, 854, 1099
BccI CCATC 2 cut(s) 655, 924
BcgI CGANNNNNNTGC 4 cut(s) 668, 702, 1094, 1128
BciT130I CCWGG 5 cut(s) 366, 396, 557, 705, 802
BcnI CCSGG 1 cut(s) 793
BcoDI GTCTC 2 cut(s) 162, 607
BfaI CTAG 2 cut(s) 510, 740
BisI GCNGC 6 cut(s) 296, 308, 717, 732, 843, 1113
BlsI GCNGC 6 cut(s) 297, 309, 718, 733, 844, 1114
Bme1390I CCNGG 6 cut(s) 366, 396, 557, 705, 793, 802
Bme18I GGWCC 5 cut(s) 362, 400, 552, 913, 963
BmgT120I GGNCC 6 cut(s) 362, 392, 400, 552, 913, 963
BmrFI CCNGG 6 cut(s) 366, 396, 557, 705, 793, 802
BmsI GCATC 2 cut(s) 223, 496
BpiI GAAGAC 3 cut(s) 24, 36, 367
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BpmI CTGGAG 2 cut(s) 618, 784
BpuMI CCSGG 1 cut(s) 793
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 4 cut(s) 394, 395, 555, 861
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bse1I ACTGG 3 cut(s) 172, 394, 639
Bse3DI GCAATG 2 cut(s) 264, 376
BseBI CCWGG 5 cut(s) 366, 396, 557, 705, 802
BseDI CCNNGG 4 cut(s) 394, 395, 555, 861
BseMI GCAATG 2 cut(s) 264, 376
BseMII CTCAG 1 cut(s) 572
BseNI ACTGG 3 cut(s) 172, 394, 639
BseRI GAGGAG 5 cut(s) 360, 363, 401, 575, 671
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 6 cut(s) 282, 294, 703, 743, 854, 1099
BseYI CCCAGC 1 cut(s) 536
BshFI GGCC 2 cut(s) 393, 1090
BshNI GGYRCC 2 cut(s) 159, 540
BsiSI CCGG 1 cut(s) 792
BslFI GGGAC 2 cut(s) 413, 976
BsmAI GTCTC 2 cut(s) 162, 607
BsmFI GGGAC 2 cut(s) 413, 976
BsmI GAATGC 2 cut(s) 145, 844
BsnI GGCC 2 cut(s) 393, 1090
Bso31I GGTCTC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 798
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 4 cut(s) 628, 744, 817, 1003
BspANI GGCC 2 cut(s) 393, 1090
BspCNI CTCAG 1 cut(s) 573
BspPI GGATC 6 cut(s) 623, 636, 739, 752, 812, 1011
BspT107I GGYRCC 2 cut(s) 159, 540
BspTNI GGTCTC 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 264, 376
BsrGI TGTACA 1 cut(s) 436
BsrI ACTGG 3 cut(s) 172, 394, 639
BssECI CCNNGG 4 cut(s) 394, 395, 555, 861
BssMI GATC 4 cut(s) 628, 744, 817, 1003
BssNAI GTATAC 1 cut(s) 871
BssT1I CCWWGG 1 cut(s) 861
Bst1107I GTATAC 1 cut(s) 871
Bst2UI CCWGG 5 cut(s) 366, 396, 557, 705, 802
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 839
BstAUI TGTACA 1 cut(s) 436
BstDEI CTNAG 2 cut(s) 287, 581
BstHHI GCGC 1 cut(s) 981
BstKTI GATC 4 cut(s) 631, 747, 820, 1006
BstMAI GTCTC 2 cut(s) 162, 607
BstMBI GATC 4 cut(s) 628, 744, 817, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 292, 304, 506, 839
BstNI CCWGG 5 cut(s) 366, 396, 557, 705, 802
BstSCI CCNGG 6 cut(s) 364, 394, 555, 703, 791, 800
BstSLI GKGCMC 1 cut(s) 798
BstV1I GCAGC 6 cut(s) 282, 294, 703, 743, 854, 1099
BstV2I GAAGAC 3 cut(s) 24, 36, 367
BstX2I RGATCY 3 cut(s) 628, 744, 1003
BstYI RGATCY 3 cut(s) 628, 744, 1003
BstZ17I GTATAC 1 cut(s) 871
BsuRI GGCC 2 cut(s) 393, 1090
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 4 cut(s) 179, 387, 632, 711
CaiI CAGNNNCTG 1 cut(s) 307
CfoI GCGC 1 cut(s) 981
Cfr13I GGNCC 6 cut(s) 362, 392, 400, 552, 913, 963
Csp6I GTAC 3 cut(s) 160, 437, 541
CspCI CAANNNNNGTGG 4 cut(s) 846, 881, 1080, 1115
CviAII CATG 5 cut(s) 482, 569, 874, 920, 982
CviQI GTAC 3 cut(s) 160, 437, 541
DdeI CTNAG 2 cut(s) 287, 581
DpnI GATC 4 cut(s) 630, 746, 819, 1005
DpnII GATC 4 cut(s) 628, 744, 817, 1003
EaeI YGGCCR 1 cut(s) 1088
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 5 cut(s) 362, 400, 552, 913, 963
Eco57I CTGAAG 3 cut(s) 284, 612, 1019
EcoO109I RGGNCCY 2 cut(s) 400, 552
EcoRI GAATTC 1 cut(s) 684
EcoRII CCWGG 5 cut(s) 364, 394, 555, 703, 800
EcoT14I CCWWGG 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 5 cut(s) 485, 572, 877, 923, 985
FaqI GGGAC 2 cut(s) 413, 976
FatI CATG 5 cut(s) 481, 568, 873, 919, 981
FblI GTMKAC 2 cut(s) 870, 884
Fnu4HI GCNGC 6 cut(s) 296, 308, 717, 732, 843, 1113
Fsp4HI GCNGC 6 cut(s) 296, 308, 717, 732, 843, 1113
FspBI CTAG 2 cut(s) 510, 740
GlaI GCGC 1 cut(s) 980
GluI GCNGC 6 cut(s) 296, 308, 717, 732, 843, 1113
GsaI CCCAGC 1 cut(s) 540
GsuI CTGGAG 2 cut(s) 618, 784
HaeIII GGCC 2 cut(s) 393, 1090
HapII CCGG 1 cut(s) 792
HhaI GCGC 1 cut(s) 981
Hin1II CATG 5 cut(s) 485, 572, 877, 923, 985
Hin6I GCGC 1 cut(s) 979
HinP1I GCGC 1 cut(s) 979
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HinfI GANTC 3 cut(s) 239, 854, 886
HpaII CCGG 1 cut(s) 792
HphI GGTGA 2 cut(s) 1032, 1071
Hpy166II GTNNAC 3 cut(s) 362, 871, 885
Hpy188I TCNGA 5 cut(s) 59, 244, 431, 505, 789
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 3 cut(s) 362, 871, 885
HpyAV CCTTC 7 cut(s) 126, 323, 415, 639, 799, 903, 994
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4V TGCA 8 cut(s) 274, 673, 694, 770, 842, 950, 992, 1073
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 304, 506, 839
HpyF3I CTNAG 2 cut(s) 287, 581
Hsp92II CATG 5 cut(s) 485, 572, 877, 923, 985
HspAI GCGC 1 cut(s) 979
KflI GGGWCCC 1 cut(s) 400
KpnI GGTACC 2 cut(s) 163, 544
Kzo9I GATC 4 cut(s) 628, 744, 817, 1003
LmnI GCTCC 4 cut(s) 98, 196, 599, 1124
Lsp1109I GCAGC 6 cut(s) 282, 294, 703, 743, 854, 1099
LweI GCATC 2 cut(s) 223, 496
MaeI CTAG 2 cut(s) 510, 740
MaeIII GTNAC 1 cut(s) 276
MalI GATC 4 cut(s) 630, 746, 819, 1005
MboI GATC 4 cut(s) 628, 744, 817, 1003
MboII GAAGA 4 cut(s) 24, 36, 328, 367
MfeI CAATTG 1 cut(s) 191
MflI RGATCY 3 cut(s) 628, 744, 1003
MhlI GDGCHC 1 cut(s) 798
MlsI TGGCCA 1 cut(s) 1090
MluCI AATT 4 cut(s) 67, 191, 684, 1017
MluNI TGGCCA 1 cut(s) 1090
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 2 cut(s) 468, 1102
Mox20I TGGCCA 1 cut(s) 1090
MscI TGGCCA 1 cut(s) 1090
MseI TTAA 2 cut(s) 74, 752
MslI CAYNNNNRTG 2 cut(s) 762, 1084
Msp20I TGGCCA 1 cut(s) 1090
MspA1I CMGCKG 2 cut(s) 845, 944
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 6 cut(s) 366, 396, 557, 705, 793, 802
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 2 cut(s) 145, 844
MvaI CCWGG 5 cut(s) 366, 396, 557, 705, 802
MwoI GCNNNNNNNGC 4 cut(s) 292, 304, 506, 839
NciI CCSGG 1 cut(s) 793
NdeII GATC 4 cut(s) 628, 744, 817, 1003
NlaIII CATG 5 cut(s) 485, 572, 877, 923, 985
OliI CACNNNNGTG 2 cut(s) 762, 1084
PasI CCCWGGG 1 cut(s) 395
PctI GAATGC 2 cut(s) 145, 844
PfeI GAWTC 2 cut(s) 239, 854
PfoI TCCNGGA 1 cut(s) 800
PkrI GCNGC 6 cut(s) 297, 309, 718, 733, 844, 1114
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
PpuMI RGGWCCY 2 cut(s) 400, 552
Psp5II RGGWCCY 2 cut(s) 400, 552
Psp6I CCWGG 5 cut(s) 364, 394, 555, 703, 800
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 5 cut(s) 364, 394, 555, 703, 800
PspPI GGNCC 6 cut(s) 362, 392, 400, 552, 913, 963
PspPPI RGGWCCY 2 cut(s) 400, 552
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 3 cut(s) 628, 744, 1003
PvuII CAGCTG 2 cut(s) 845, 944
RsaI GTAC 3 cut(s) 161, 438, 542
RsaNI GTAC 3 cut(s) 160, 437, 541
RseI CAYNNNNRTG 2 cut(s) 762, 1084
SalI GTCGAC 1 cut(s) 883
SaqAI TTAA 2 cut(s) 74, 752
SatI GCNGC 6 cut(s) 296, 308, 717, 732, 843, 1113
Sau3AI GATC 4 cut(s) 628, 744, 817, 1003
Sau96I GGNCC 6 cut(s) 362, 392, 400, 552, 913, 963
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 6 cut(s) 366, 396, 557, 705, 793, 802
SduI GDGCHC 1 cut(s) 798
SfaNI GCATC 2 cut(s) 223, 496
SinI GGWCC 5 cut(s) 362, 400, 552, 913, 963
SmiMI CAYNNNNRTG 2 cut(s) 762, 1084
Sse9I AATT 4 cut(s) 67, 191, 684, 1017
SspMI CTAG 2 cut(s) 510, 740
StyD4I CCNGG 6 cut(s) 364, 394, 555, 703, 791, 800
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 1 cut(s) 607
TaqI TCGA 2 cut(s) 688, 884
TasI AATT 4 cut(s) 67, 191, 684, 1017
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 2 cut(s) 239, 854
Tru1I TTAA 2 cut(s) 74, 752
Tru9I TTAA 2 cut(s) 74, 752
TscAI CASTG 4 cut(s) 179, 394, 639, 718
TseI GCWGC 6 cut(s) 295, 307, 716, 731, 842, 1112
TspDTI ATGAA 2 cut(s) 585, 1029
TspRI CASTG 4 cut(s) 179, 394, 639, 718
VpaK11BI GGWCC 5 cut(s) 362, 400, 552, 913, 963
XapI RAATTY 1 cut(s) 684
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 2 cut(s) 870, 884
XspI CTAG 2 cut(s) 510, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.