RchiOBHm_Chr1g0355121

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003309
Physical Location & Seq
Reverse (-)
47861364 .. 47867884
6521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGACTCCGAATCAGTGATTGAGTTTCTGGGCTGCGTTCCTCTGCTTCAGAGACTACCCAGCTCCTCGCTCAAGAAAATCGCCCAGCTTGTAATTTTAAAGCACTACGAAAAAGGAGAGTATGTTGTTCGTGAAGGGGAGGTTGGGATTGGGGTTTACTTCATTTGGGAAGGAGAGGCTGAAGTTACTGGGTCTGTTAATGCTGATGAAGACAATCGGCCGGAGTTTCAGTTGAAGCGATATGATTACTTTGGTTATGGTTTTAAAGCATTAGGTCATCAAGCTGATGTAATTGCTTCATCTAAGCTGACATGCTTGGTGCTACCCCATGAACATTATTCTCTGCTGCAGCCAAAATCAATCTGGCATGCTGATAAAGCTCTTGAGACATGCTCTCTTGTTGAGAATATATTGCATTTGGAGCCAATTGAAGCAAATATATTTCAGGGGATTACTTTACCGGAGGCACCAAGATTTGGACAGGTTTTCGGAGGACAGTTAGCGTTGGCCGCAGCAACAAAAACTGTTGATTGTCTCAAAGTCGTCCATAGTTTGCATGCACATTTTTTGCTTGTCGGGGATTTCAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGAAAGAGCTTTGCTACTCGAAGAGTAGATGCAATACAAAAAGGGATAAACATATTCACTTTACTGGCTTCCTTTCAAAAAGAAGAAGAAGGTTCTGTCCACCAGGAAGCCATAATGCCATCTGTGCCTCCCCCAGATACGCTCCCATCAATGGAAGAACTGCGCGAAAGACGCCTTTTTGATCCCCGTCTTCCAAGAACTTATCGAATCAAGGTAGCTGCAAAGGAGTTTGTCCCGTGGCCAATTGAGATACGATTTTGTGAACCTAGTAGCCGGACCAATCAGACTAAATCTCCACCAAGATTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTGGCTTATGCTTCAGATTTGATATTCGCTGGTGTTAGTCTGAATCCTCACCGTAGAAGAGGACATAAAACTAGTCAAGTTAGTTTGGACCATGCAATGTGGTTTCACCGCCCCCTTAGAGCTGATGACTGGTTACTACATGTGATAGTGAGTCCCATTGCCTATAATGCACGCGGCTATGTCACAGGCCAAATGTTCAACACCAAGGGAGAGCTTGTTGTATCATTGACTCAAGAGTCCCTGAATAGGCCAATTAGACCAACTCCGGCTGTCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

425

Amino Acids

48.09

Weight (kDa)

8.61

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 34 - 112 3.1e-08 Cyclic nucleotide-binding domain
4HBT_3 PF13622 159 - 234 5e-14 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 299 - 410 1.9e-23 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 303 - 409 5.5e-24 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1225
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 476
AccII CGCG 2 cut(s) 789, 1164
AciI CCGC 3 cut(s) 510, 1099, 1164
AclWI GGATC 1 cut(s) 800
AcoI YGGCCR 3 cut(s) 218, 507, 863
AcuI CTGAAG 3 cut(s) 32, 201, 987
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 3 cut(s) 476, 775, 1236
AflIII ACRYGT 1 cut(s) 1129
AgsI TTSAA 5 cut(s) 235, 431, 586, 701, 1189
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 1 cut(s) 726
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 9 cut(s) 63, 88, 284, 307, 380, 633, 842, 1112, 1204
AluI AGCT 9 cut(s) 63, 88, 284, 307, 380, 633, 842, 1112, 1204
Alw26I GTCTC 3 cut(s) 46, 380, 539
AlwI GGATC 1 cut(s) 800
AoxI GGCC 5 cut(s) 218, 507, 863, 1177, 1238
ApeKI GCWGC 5 cut(s) 33, 346, 349, 512, 842
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 1 cut(s) 789
AspS9I GGNCC 2 cut(s) 900, 1078
AsuHPI GGTGA 3 cut(s) 602, 1031, 1088
AvaII GGWCC 2 cut(s) 900, 1078
BalI TGGCCA 1 cut(s) 865
BanI GGYRCC 1 cut(s) 466
BbsI GAAGAC 2 cut(s) 216, 806
BbvI GCAGC 5 cut(s) 20, 333, 361, 524, 829
BccI CCATC 3 cut(s) 617, 751, 778
BciT130I CCWGG 1 cut(s) 728
BclI TGATCA 1 cut(s) 967
BcoDI GTCTC 3 cut(s) 46, 380, 539
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 2 cut(s) 891, 1062
BfmI CTRYAG 1 cut(s) 347
BisI GCNGC 7 cut(s) 34, 347, 350, 510, 513, 843, 1165
BlsI GCNGC 7 cut(s) 35, 348, 351, 511, 514, 844, 1166
Bme1390I CCNGG 1 cut(s) 728
Bme18I GGWCC 2 cut(s) 900, 1078
BmgT120I GGNCC 2 cut(s) 900, 1078
BmiI GGNNCC 2 cut(s) 423, 468
BmrFI CCNGG 1 cut(s) 728
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 643
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 216, 806
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpuEI CTTGAG 3 cut(s) 56, 404, 1206
BsaAI YACGTR 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 2 cut(s) 860, 1194
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 8 cut(s) 108, 138, 215, 245, 901, 931, 1191, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 775, 1236
Bse1I ACTGG 3 cut(s) 193, 693, 1124
Bse3DI GCAATG 2 cut(s) 1092, 1146
BseBI CCWGG 1 cut(s) 728
BseDI CCNNGG 2 cut(s) 860, 1194
BseLI CCNNNNNNNGG 3 cut(s) 476, 775, 1236
BseMI GCAATG 2 cut(s) 1092, 1146
BseNI ACTGG 3 cut(s) 193, 693, 1124
BseRI GAGGAG 1 cut(s) 55
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 5 cut(s) 20, 333, 361, 524, 829
BseYI CCCAGC 2 cut(s) 59, 84
Bsh1236I CGCG 2 cut(s) 789, 1164
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 5 cut(s) 220, 509, 865, 1179, 1240
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 221
BsiSI CCGG 4 cut(s) 221, 461, 898, 1256
BslFI GGGAC 3 cut(s) 842, 1128, 1213
BslI CCNNNNNNNGG 3 cut(s) 476, 775, 1236
BsmAI GTCTC 3 cut(s) 46, 380, 539
BsmFI GGGAC 3 cut(s) 842, 1128, 1213
BsnI GGCC 5 cut(s) 220, 509, 865, 1179, 1240
Bsp143I GATC 2 cut(s) 805, 967
BspACI CCGC 3 cut(s) 510, 1099, 1164
BspANI GGCC 5 cut(s) 220, 509, 865, 1179, 1240
BspFNI CGCG 2 cut(s) 789, 1164
BspLI GGNNCC 2 cut(s) 423, 468
BspMAI CTGCAG 1 cut(s) 351
BspPI GGATC 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 2 cut(s) 1092, 1146
BsrI ACTGG 3 cut(s) 193, 693, 1124
BssECI CCNNGG 2 cut(s) 860, 1194
BssMI GATC 2 cut(s) 805, 967
BssNI GRCGYC 1 cut(s) 796
BssT1I CCWWGG 1 cut(s) 1194
Bst2UI CCWGG 1 cut(s) 728
Bst4CI ACNGT 3 cut(s) 498, 526, 1043
Bst6I CTCTTC 2 cut(s) 640, 1042
BstACI GRCGYC 1 cut(s) 796
BstBAI YACGTR 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 369, 558, 1162
BstDEI CTNAG 3 cut(s) 303, 1106, 1269
BstDSI CCRYGG 1 cut(s) 860
BstFNI CGCG 2 cut(s) 789, 1164
BstHHI GCGC 1 cut(s) 789
BstKTI GATC 2 cut(s) 808, 970
BstMAI GTCTC 3 cut(s) 46, 380, 539
BstMBI GATC 2 cut(s) 805, 967
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 6 cut(s) 377, 421, 509, 748, 795, 1157
BstNI CCWGG 1 cut(s) 728
BstNSI RCATGY 5 cut(s) 315, 371, 393, 560, 1133
BstSCI CCNGG 1 cut(s) 726
BstSFI CTRYAG 1 cut(s) 347
BstUI CGCG 2 cut(s) 789, 1164
BstV1I GCAGC 5 cut(s) 20, 333, 361, 524, 829
BstV2I GAAGAC 2 cut(s) 216, 806
BstZI CGGCCG 1 cut(s) 218
BsuRI GGCC 5 cut(s) 220, 509, 865, 1179, 1240
BtgI CCRYGG 1 cut(s) 860
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 3 cut(s) 369, 558, 1162
CfoI GCGC 1 cut(s) 789
Cfr13I GGNCC 2 cut(s) 900, 1078
CseI GACGC 1 cut(s) 804
Csp6I GTAC 1 cut(s) 617
CspCI CAANNNNNGTGG 2 cut(s) 909, 944
CviAII CATG 7 cut(s) 312, 329, 368, 390, 557, 1082, 1130
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 3 cut(s) 303, 1106, 1269
DpnI GATC 2 cut(s) 807, 969
DpnII GATC 2 cut(s) 805, 967
DraI TTTAAA 2 cut(s) 99, 265
DrdI GACNNNNNNGTC 1 cut(s) 1225
DseDI GACNNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 3 cut(s) 218, 507, 863
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 2 cut(s) 640, 1042
EarI CTCTTC 2 cut(s) 640, 1042
EclXI CGGCCG 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 1194
Eco47I GGWCC 2 cut(s) 900, 1078
Eco52I CGGCCG 1 cut(s) 218
Eco57I CTGAAG 3 cut(s) 32, 201, 987
EcoRII CCWGG 1 cut(s) 726
EcoT14I CCWWGG 1 cut(s) 1194
ErhI CCWWGG 1 cut(s) 1194
FaeI CATG 7 cut(s) 315, 332, 371, 393, 560, 1085, 1133
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FaqI GGGAC 3 cut(s) 842, 1128, 1213
FatI CATG 7 cut(s) 311, 328, 367, 389, 556, 1081, 1129
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 7 cut(s) 34, 347, 350, 510, 513, 843, 1165
Fsp4HI GCNGC 7 cut(s) 34, 347, 350, 510, 513, 843, 1165
FspBI CTAG 2 cut(s) 891, 1062
GlaI GCGC 1 cut(s) 788
GluI GCNGC 7 cut(s) 34, 347, 350, 510, 513, 843, 1165
GsaI CCCAGC 2 cut(s) 63, 88
HaeIII GGCC 5 cut(s) 220, 509, 865, 1179, 1240
HapII CCGG 4 cut(s) 221, 461, 898, 1256
HgaI GACGC 1 cut(s) 804
HhaI GCGC 1 cut(s) 789
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 7 cut(s) 315, 332, 371, 393, 560, 1085, 1133
Hin6I GCGC 1 cut(s) 787
HinP1I GCGC 1 cut(s) 787
HinfI GANTC 7 cut(s) 5, 11, 831, 1033, 1141, 1219, 1226
HpaII CCGG 4 cut(s) 221, 461, 898, 1256
HphI GGTGA 3 cut(s) 602, 1031, 1088
Hpy166II GTNNAC 4 cut(s) 157, 610, 724, 887
Hpy188I TCNGA 7 cut(s) 10, 51, 491, 909, 964, 1006, 1032
Hpy188III TCNNGA 4 cut(s) 73, 131, 383, 1223
Hpy8I GTNNAC 4 cut(s) 157, 610, 724, 887
HpyAV CCTTC 3 cut(s) 128, 164, 707
HpyCH4III ACNGT 3 cut(s) 498, 526, 1043
HpyCH4IV ACGT 1 cut(s) 619
HpyCH4V TGCA 9 cut(s) 349, 415, 556, 560, 656, 845, 979, 1085, 1160
HpyF10VI GCNNNNNNNGC 6 cut(s) 377, 421, 509, 748, 795, 1157
HpyF3I CTNAG 3 cut(s) 303, 1106, 1269
HpySE526I ACGT 1 cut(s) 619
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 7 cut(s) 315, 332, 371, 393, 560, 1085, 1133
HspAI GCGC 1 cut(s) 787
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 2 cut(s) 805, 967
LmnI GCTCC 3 cut(s) 68, 421, 771
Lsp1109I GCAGC 5 cut(s) 20, 333, 361, 524, 829
LweI GCATC 1 cut(s) 643
MaeI CTAG 2 cut(s) 891, 1062
MaeII ACGT 1 cut(s) 619
MaeIII GTNAC 4 cut(s) 184, 1122, 1171, 1261
MalI GATC 2 cut(s) 807, 969
MboI GATC 2 cut(s) 805, 967
MboII GAAGA 7 cut(s) 221, 657, 719, 722, 791, 806, 1059
MfeI CAATTG 2 cut(s) 426, 867
MlsI TGGCCA 1 cut(s) 865
MluCI AATT 6 cut(s) 93, 291, 426, 594, 867, 1242
MluNI TGGCCA 1 cut(s) 865
MlyI GAGTC 3 cut(s) 1150, 1213, 1235
Mox20I TGGCCA 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 958
MscI TGGCCA 1 cut(s) 865
MseI TTAA 3 cut(s) 98, 198, 264
MslI CAYNNNNRTG 1 cut(s) 984
Msp20I TGGCCA 1 cut(s) 865
MspI CCGG 4 cut(s) 221, 461, 898, 1256
MspR9I CCNGG 1 cut(s) 728
MunI CAATTG 2 cut(s) 426, 867
MvaI CCWGG 1 cut(s) 728
MvnI CGCG 2 cut(s) 789, 1164
MwoI GCNNNNNNNGC 6 cut(s) 377, 421, 509, 748, 795, 1157
NdeII GATC 2 cut(s) 805, 967
NlaIII CATG 7 cut(s) 315, 332, 371, 393, 560, 1085, 1133
NlaIV GGNNCC 2 cut(s) 423, 468
NmuCI GTSAC 2 cut(s) 1171, 1261
NspI RCATGY 5 cut(s) 315, 371, 393, 560, 1133
PaeI GCATGC 2 cut(s) 371, 560
PciI ACATGT 1 cut(s) 1129
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 3 cut(s) 11, 831, 1033
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 7 cut(s) 35, 348, 351, 511, 514, 844, 1166
PleI GAGTC 3 cut(s) 1149, 1213, 1234
PpsI GAGTC 3 cut(s) 1149, 1213, 1234
Ppu21I YACGTR 1 cut(s) 620
PscI ACATGT 1 cut(s) 1129
Psp6I CCWGG 1 cut(s) 726
PspFI CCCAGC 2 cut(s) 59, 84
PspGI CCWGG 1 cut(s) 726
PspN4I GGNNCC 2 cut(s) 423, 468
PspPI GGNCC 2 cut(s) 900, 1078
PstI CTGCAG 1 cut(s) 351
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
RseI CAYNNNNRTG 1 cut(s) 984
SaqAI TTAA 3 cut(s) 98, 198, 264
SatI GCNGC 7 cut(s) 34, 347, 350, 510, 513, 843, 1165
Sau3AI GATC 2 cut(s) 805, 967
Sau96I GGNCC 2 cut(s) 900, 1078
SchI GAGTC 3 cut(s) 1150, 1213, 1235
ScrFI CCNGG 1 cut(s) 728
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 347
SinI GGWCC 2 cut(s) 900, 1078
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 3 cut(s) 71, 383, 1221
SmoI CTYRAG 3 cut(s) 71, 383, 1221
SpeI ACTAGT 1 cut(s) 1061
SphI GCATGC 2 cut(s) 371, 560
Sse9I AATT 6 cut(s) 93, 291, 426, 594, 867, 1242
SsiI CCGC 3 cut(s) 510, 1099, 1164
SspMI CTAG 2 cut(s) 891, 1062
StyD4I CCNGG 1 cut(s) 726
StyI CCWWGG 1 cut(s) 1194
TaaI ACNGT 3 cut(s) 498, 526, 1043
TaiI ACGT 1 cut(s) 622
TaqI TCGA 2 cut(s) 643, 829
TasI AATT 6 cut(s) 93, 291, 426, 594, 867, 1242
TauI GCSGC 2 cut(s) 512, 1167
TfiI GAWTC 3 cut(s) 11, 831, 1033
Tru1I TTAA 3 cut(s) 98, 198, 264
Tru9I TTAA 3 cut(s) 98, 198, 264
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 2 cut(s) 1171, 1261
TseI GCWGC 5 cut(s) 33, 346, 349, 512, 842
Tsp45I GTSAC 2 cut(s) 1171, 1261
TspDTI ATGAA 4 cut(s) 151, 222, 288, 345
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 900, 1078
XceI RCATGY 5 cut(s) 315, 371, 393, 560, 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 2 cut(s) 891, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.