RchiOBHm_Chr1g0382911

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004548
Physical Location & Seq
Reverse (-)
67493728 .. 67494300
573 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGGATCAACTGAGCAACCTAGCTGTACTAATTTGTCATCGTCTCTTTCTATTCATGAAGAAGATGATCGTATACTCATTGTTCAAACCTGAAGTTGAAGACAAGGTCGACACTAACGATCATCATCAGGTTGAGATGAATTTCTCACCTGCAGCCGCTCCGAATTGGTTGCACTTGGAGGTACCAGTAGTTCCGGTGCTAGTTGACACTGAATCGGTTAAGCAACTTCCGGTTGTGCAATACCGGAATCGAATGATGGAGAGAAGAGATCAATCGCGATATAGAGATCACGAGGATGACGAGTCTATGTGTATAGTGTGCATGAATAGCATAGAGGCTATAGATGAAGTAAGAGAGCTGAAGAACTGTTCCCACGTCTATCATAGAGAGTGCTTGGATCTTTGGGTTGCTCAGGGTCAGGTAACCTGTCCTCTGTGCAGGTCTAAGCTATTGCCAACTGCTAGGGCTACGCCAAATGAACATTTGAAACCTGCGGGAGGGGATCCATGGAGATCGGAGAGGATGATTTACTTGTTCGGTGATGATTGCTTCAAGCACTGGATAGACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

190

Amino Acids

22.27

Weight (kDa)

5.57

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 102 - 147 4.4e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 103 - 147 1e-10 Ring finger domain
zf-C3HC4_2 PF13923 103 - 146 2.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 104 - 146 4.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 104 - 132 1.3e-06 RING-like zinc finger
zf-RING_UBOX PF13445 104 - 144 8.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 3 cut(s) 157, 429, 499
Acc65I GGTACC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 2 cut(s) 72, 108
AccII CGCG 1 cut(s) 277
AciI CCGC 2 cut(s) 156, 494
AclWI GGATC 4 cut(s) 12, 405, 497, 510
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 111, 380
AfaI GTAC 2 cut(s) 27, 183
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 4 cut(s) 85, 98, 487, 553
AjiI CACGTC 1 cut(s) 376
AluBI AGCT 3 cut(s) 23, 358, 448
AluI AGCT 3 cut(s) 23, 358, 448
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 4 cut(s) 12, 405, 497, 510
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 139
Asp718I GGTACC 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 138, 551
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 181
BauI CACGAG 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 3 cut(s) 20, 200, 462
BfmI CTRYAG 2 cut(s) 150, 339
BfuAI ACCTGC 3 cut(s) 157, 429, 499
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
BmgBI CACGTC 1 cut(s) 376
BmiI GGNNCC 2 cut(s) 183, 504
BpiI GAAGAC 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 506
BsaWI WCCGGW 3 cut(s) 193, 229, 243
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse1I ACTGG 2 cut(s) 185, 563
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 2 cut(s) 301, 528
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 2 cut(s) 185, 563
BseXI GCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 457
Bsh1236I CGCG 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 181
BsiSI CCGG 3 cut(s) 194, 230, 244
BslI CCNNNNNNNGG 1 cut(s) 497
BsmAI GTCTC 1 cut(s) 47
BsmBI CGTCTC 1 cut(s) 47
Bsp143I GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
Bsp19I CCATGG 1 cut(s) 506
Bsp68I TCGCGA 1 cut(s) 277
BspACI CCGC 2 cut(s) 156, 494
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 277
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 183, 504
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 3 cut(s) 157, 429, 499
BspPI GGATC 4 cut(s) 12, 405, 497, 510
BspT107I GGYRCC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 158
BsrI ACTGG 2 cut(s) 185, 563
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
BssNAI GTATAC 1 cut(s) 73
BssSI CACGAG 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 506
Bst1107I GTATAC 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 1 cut(s) 259
BstDEI CTNAG 3 cut(s) 11, 411, 444
BstDSI CCRYGG 1 cut(s) 506
BstEII GGTNACC 1 cut(s) 421
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 301, 528
BstFNI CGCG 1 cut(s) 277
BstKTI GATC 8 cut(s) 7, 69, 121, 271, 289, 400, 505, 515
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstPI GGTNACC 1 cut(s) 421
BstSFI CTRYAG 2 cut(s) 150, 339
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 397, 502
BstYI RGATCY 2 cut(s) 397, 502
BstZ17I GTATAC 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 506
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 2 cut(s) 301, 528
BtsIMutI CAGTG 2 cut(s) 207, 556
BtuMI TCGCGA 1 cut(s) 277
BveI ACCTGC 3 cut(s) 157, 429, 499
CciI TCATGA 1 cut(s) 54
Csp6I GTAC 2 cut(s) 26, 182
CviAII CATG 3 cut(s) 55, 322, 507
CviJI RGCY 6 cut(s) 23, 155, 338, 358, 448, 467
CviKI_1 RGCY 6 cut(s) 23, 155, 338, 358, 448, 467
CviQI GTAC 2 cut(s) 26, 182
DdeI CTNAG 3 cut(s) 11, 411, 444
DpnI GATC 8 cut(s) 6, 68, 120, 270, 288, 399, 504, 514
DpnII GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 506
Eco57I CTGAAG 2 cut(s) 111, 380
Eco91I GGTNACC 1 cut(s) 421
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoO65I GGTNACC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 3 cut(s) 58, 325, 510
FatI CATG 3 cut(s) 54, 321, 506
FauI CCCGC 1 cut(s) 487
FblI GTMKAC 2 cut(s) 72, 108
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 2 cut(s) 308, 535
Fsp4HI GCNGC 2 cut(s) 153, 156
FspBI CTAG 3 cut(s) 20, 200, 462
GluI GCNGC 2 cut(s) 153, 156
HapII CCGG 3 cut(s) 194, 230, 244
Hin1II CATG 3 cut(s) 58, 325, 510
HincII GTYRAC 2 cut(s) 109, 205
HindII GTYRAC 2 cut(s) 109, 205
HinfI GANTC 3 cut(s) 212, 247, 302
HpaII CCGG 3 cut(s) 194, 230, 244
HphI GGTGA 2 cut(s) 138, 551
Hpy166II GTNNAC 3 cut(s) 73, 109, 205
Hpy188I TCNGA 2 cut(s) 162, 517
Hpy188III TCNNGA 3 cut(s) 55, 276, 290
Hpy8I GTNNAC 3 cut(s) 73, 109, 205
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 152, 172, 238, 321, 438
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpyF3I CTNAG 3 cut(s) 11, 411, 444
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 3 cut(s) 58, 325, 510
KpnI GGTACC 1 cut(s) 185
Kzo9I GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
LmnI GCTCC 1 cut(s) 163
Lsp1109I GCAGC 1 cut(s) 164
MaeI CTAG 3 cut(s) 20, 200, 462
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 421
MalI GATC 8 cut(s) 6, 68, 120, 270, 288, 399, 504, 514
MbiI CCGCTC 1 cut(s) 158
MboI GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
MboII GAAGA 5 cut(s) 70, 73, 110, 276, 373
MflI RGATCY 2 cut(s) 397, 502
MluCI AATT 3 cut(s) 30, 139, 163
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 6 cut(s) 172, 286, 328, 441, 491, 513
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 294
MspI CCGG 3 cut(s) 194, 230, 244
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 1 cut(s) 327
NcoI CCATGG 1 cut(s) 506
NdeII GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
NlaIII CATG 3 cut(s) 58, 325, 510
NlaIV GGNNCC 2 cut(s) 183, 504
NruI TCGCGA 1 cut(s) 277
PagI TCATGA 1 cut(s) 54
PaqCI CACCTGC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 297
PfeI GAWTC 2 cut(s) 212, 247
PflFI GACNNNGTC 1 cut(s) 104
PkrI GCNGC 2 cut(s) 154, 157
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PspEI GGTNACC 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 183, 504
PsrI GAACNNNNNNTAC 2 cut(s) 174, 206
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 2 cut(s) 397, 502
PsyI GACNNNGTC 1 cut(s) 104
RruI TCGCGA 1 cut(s) 277
RsaI GTAC 2 cut(s) 27, 183
RsaNI GTAC 2 cut(s) 26, 182
RseI CAYNNNNRTG 1 cut(s) 294
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 8 cut(s) 4, 66, 118, 268, 286, 397, 502, 512
SchI GAGTC 1 cut(s) 311
SfcI CTRYAG 2 cut(s) 150, 339
SmiMI CAYNNNNRTG 1 cut(s) 294
Sse9I AATT 3 cut(s) 30, 139, 163
SsiI CCGC 2 cut(s) 156, 494
SspMI CTAG 3 cut(s) 20, 200, 462
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 1 cut(s) 378
TaqI TCGA 2 cut(s) 108, 250
TasI AATT 3 cut(s) 30, 139, 163
TatI WGTACW 1 cut(s) 25
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 212, 247
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 2 cut(s) 214, 563
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 6 cut(s) 43, 71, 152, 338, 360, 492
TspRI CASTG 2 cut(s) 214, 563
Tth111I GACNNNGTC 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 1 cut(s) 139
XmiI GTMKAC 2 cut(s) 72, 108
XspI CTAG 3 cut(s) 20, 200, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.