DNA-directed RNA polymerases I, II, and III subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 173 |
| AclWI | GGATC | 1 cut(s) | 437 |
| AcoI | YGGCCR | 1 cut(s) | 497 |
| AcsI | RAATTY | 1 cut(s) | 313 |
| AfaI | GTAC | 2 cut(s) | 302, 549 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 173 |
| AflII | CTTAAG | 1 cut(s) | 467 |
| AgsI | TTSAA | 2 cut(s) | 248, 297 |
| AjnI | CCWGG | 1 cut(s) | 559 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 156, 188 |
| AluBI | AGCT | 1 cut(s) | 436 |
| AluI | AGCT | 1 cut(s) | 436 |
| Alw26I | GTCTC | 2 cut(s) | 418, 500 |
| AlwI | GGATC | 1 cut(s) | 437 |
| AoxI | GGCC | 2 cut(s) | 177, 497 |
| ApoI | RAATTY | 1 cut(s) | 313 |
| AspS9I | GGNCC | 2 cut(s) | 97, 177 |
| AsuHPI | GGTGA | 2 cut(s) | 349, 517 |
| AvaII | GGWCC | 1 cut(s) | 97 |
| BbsI | GAAGAC | 1 cut(s) | 404 |
| BccI | CCATC | 1 cut(s) | 283 |
| BciT130I | CCWGG | 1 cut(s) | 561 |
| BclI | TGATCA | 2 cut(s) | 31, 136 |
| BcoDI | GTCTC | 2 cut(s) | 418, 500 |
| BfaI | CTAG | 1 cut(s) | 359 |
| BfmI | CTRYAG | 1 cut(s) | 510 |
| BfrI | CTTAAG | 1 cut(s) | 467 |
| Bme1390I | CCNGG | 1 cut(s) | 561 |
| Bme18I | GGWCC | 1 cut(s) | 97 |
| BmgT120I | GGNCC | 2 cut(s) | 97, 177 |
| BmiI | GGNNCC | 1 cut(s) | 178 |
| BmrFI | CCNGG | 1 cut(s) | 561 |
| BmsI | GCATC | 1 cut(s) | 379 |
| BpiI | GAAGAC | 1 cut(s) | 404 |
| BpmI | CTGGAG | 1 cut(s) | 427 |
| Bpu10I | CCTNAGC | 1 cut(s) | 366 |
| BsaBI | GATNNNNATC | 1 cut(s) | 36 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 173 |
| Bse8I | GATNNNNATC | 1 cut(s) | 36 |
| BseBI | CCWGG | 1 cut(s) | 561 |
| BseJI | GATNNNNATC | 1 cut(s) | 36 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 173 |
| BseMII | CTCAG | 1 cut(s) | 357 |
| BshFI | GGCC | 2 cut(s) | 179, 499 |
| BsiSI | CCGG | 1 cut(s) | 496 |
| BslFI | GGGAC | 1 cut(s) | 29 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 173 |
| BsmAI | GTCTC | 2 cut(s) | 418, 500 |
| BsmFI | GGGAC | 1 cut(s) | 29 |
| BsnI | GGCC | 2 cut(s) | 179, 499 |
| Bsp143I | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| BspANI | GGCC | 2 cut(s) | 179, 499 |
| BspCNI | CTCAG | 1 cut(s) | 358 |
| BspHI | TCATGA | 1 cut(s) | 34 |
| BspLI | GGNNCC | 1 cut(s) | 178 |
| BspMAI | CTGCAG | 1 cut(s) | 514 |
| BspPI | GGATC | 1 cut(s) | 437 |
| BspTI | CTTAAG | 1 cut(s) | 467 |
| BssMI | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| Bst2UI | CCWGG | 1 cut(s) | 561 |
| Bst4CI | ACNGT | 2 cut(s) | 60, 533 |
| Bst6I | CTCTTC | 2 cut(s) | 113, 185 |
| BstAFI | CTTAAG | 1 cut(s) | 467 |
| BstDEI | CTNAG | 2 cut(s) | 238, 366 |
| BstKTI | GATC | 5 cut(s) | 13, 34, 40, 139, 445 |
| BstMAI | GTCTC | 2 cut(s) | 418, 500 |
| BstMBI | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| BstNI | CCWGG | 1 cut(s) | 561 |
| BstNSI | RCATGY | 1 cut(s) | 356 |
| BstSCI | CCNGG | 1 cut(s) | 559 |
| BstSFI | CTRYAG | 1 cut(s) | 510 |
| BstV2I | GAAGAC | 1 cut(s) | 404 |
| BsuRI | GGCC | 2 cut(s) | 179, 499 |
| CciI | TCATGA | 1 cut(s) | 34 |
| Cfr13I | GGNCC | 2 cut(s) | 97, 177 |
| Csp6I | GTAC | 2 cut(s) | 301, 548 |
| CviAII | CATG | 6 cut(s) | 35, 46, 74, 85, 208, 353 |
| CviJI | RGCY | 4 cut(s) | 179, 436, 466, 499 |
| CviKI_1 | RGCY | 4 cut(s) | 179, 436, 466, 499 |
| CviQI | GTAC | 2 cut(s) | 301, 548 |
| DdeI | CTNAG | 2 cut(s) | 238, 366 |
| DpnI | GATC | 5 cut(s) | 12, 33, 39, 138, 444 |
| DpnII | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| DraI | TTTAAA | 1 cut(s) | 64 |
| EaeI | YGGCCR | 1 cut(s) | 497 |
| Eam1104I | CTCTTC | 2 cut(s) | 113, 185 |
| EarI | CTCTTC | 2 cut(s) | 113, 185 |
| Eco47I | GGWCC | 1 cut(s) | 97 |
| EcoO109I | RGGNCCY | 1 cut(s) | 97 |
| EcoRII | CCWGG | 1 cut(s) | 559 |
| FaeI | CATG | 6 cut(s) | 38, 49, 77, 88, 211, 356 |
| FaiI | YATR | 8 cut(s) | 36, 47, 75, 86, 147, 209, 354, 522 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 290, 322 |
| FaqI | GGGAC | 1 cut(s) | 29 |
| FatI | CATG | 6 cut(s) | 34, 45, 73, 84, 207, 352 |
| FbaI | TGATCA | 2 cut(s) | 31, 136 |
| FspBI | CTAG | 1 cut(s) | 359 |
| GsuI | CTGGAG | 1 cut(s) | 427 |
| HaeIII | GGCC | 2 cut(s) | 179, 499 |
| HapII | CCGG | 1 cut(s) | 496 |
| Hin1II | CATG | 6 cut(s) | 38, 49, 77, 88, 211, 356 |
| HinfI | GANTC | 4 cut(s) | 131, 211, 259, 411 |
| HpaII | CCGG | 1 cut(s) | 496 |
| HphI | GGTGA | 2 cut(s) | 349, 517 |
| Hpy166II | GTNNAC | 1 cut(s) | 476 |
| Hpy188I | TCNGA | 3 cut(s) | 136, 141, 258 |
| Hpy188III | TCNNGA | 5 cut(s) | 14, 35, 215, 320, 365 |
| Hpy8I | GTNNAC | 1 cut(s) | 476 |
| HpyAV | CCTTC | 1 cut(s) | 108 |
| HpyCH4III | ACNGT | 2 cut(s) | 60, 533 |
| HpyCH4V | TGCA | 4 cut(s) | 269, 452, 512, 568 |
| HpyF3I | CTNAG | 2 cut(s) | 238, 366 |
| Hsp92II | CATG | 6 cut(s) | 38, 49, 77, 88, 211, 356 |
| Ksp22I | TGATCA | 2 cut(s) | 31, 136 |
| Kzo9I | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| LpnPI | CCDG | 8 cut(s) | 171, 200, 305, 378, 391, 498, 509, 546 |
| LweI | GCATC | 1 cut(s) | 379 |
| MaeI | CTAG | 1 cut(s) | 359 |
| MaeIII | GTNAC | 4 cut(s) | 54, 194, 523, 533 |
| MalI | GATC | 5 cut(s) | 12, 33, 39, 138, 444 |
| MboI | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| MboII | GAAGA | 4 cut(s) | 130, 202, 409, 499 |
| MfeI | CAATTG | 1 cut(s) | 447 |
| MluCI | AATT | 5 cut(s) | 24, 225, 313, 329, 447 |
| MlyI | GAGTC | 3 cut(s) | 140, 253, 420 |
| MnlI | CCTC | 5 cut(s) | 88, 286, 316, 382, 435 |
| MseI | TTAA | 3 cut(s) | 63, 345, 468 |
| MslI | CAYNNNNRTG | 2 cut(s) | 50, 206 |
| MspA1I | CMGCKG | 1 cut(s) | 436 |
| MspCI | CTTAAG | 1 cut(s) | 467 |
| MspI | CCGG | 1 cut(s) | 496 |
| MspR9I | CCNGG | 1 cut(s) | 561 |
| MunI | CAATTG | 1 cut(s) | 447 |
| MvaI | CCWGG | 1 cut(s) | 561 |
| NdeII | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| NlaIII | CATG | 6 cut(s) | 38, 49, 77, 88, 211, 356 |
| NlaIV | GGNNCC | 1 cut(s) | 178 |
| NmuCI | GTSAC | 2 cut(s) | 54, 523 |
| NspI | RCATGY | 1 cut(s) | 356 |
| PagI | TCATGA | 1 cut(s) | 34 |
| PfeI | GAWTC | 1 cut(s) | 211 |
| PflFI | GACNNNGTC | 1 cut(s) | 52 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 173 |
| PleI | GAGTC | 3 cut(s) | 139, 253, 419 |
| PpsI | GAGTC | 3 cut(s) | 139, 253, 419 |
| PpuMI | RGGWCCY | 1 cut(s) | 97 |
| Psp5II | RGGWCCY | 1 cut(s) | 97 |
| Psp6I | CCWGG | 1 cut(s) | 559 |
| PspGI | CCWGG | 1 cut(s) | 559 |
| PspN4I | GGNNCC | 1 cut(s) | 178 |
| PspPI | GGNCC | 2 cut(s) | 97, 177 |
| PspPPI | RGGWCCY | 1 cut(s) | 97 |
| PstI | CTGCAG | 1 cut(s) | 514 |
| PsyI | GACNNNGTC | 1 cut(s) | 52 |
| PvuII | CAGCTG | 1 cut(s) | 436 |
| RsaI | GTAC | 2 cut(s) | 302, 549 |
| RsaNI | GTAC | 2 cut(s) | 301, 548 |
| RseI | CAYNNNNRTG | 2 cut(s) | 50, 206 |
| SaqAI | TTAA | 3 cut(s) | 63, 345, 468 |
| Sau3AI | GATC | 5 cut(s) | 10, 31, 37, 136, 442 |
| Sau96I | GGNCC | 2 cut(s) | 97, 177 |
| SchI | GAGTC | 3 cut(s) | 140, 253, 420 |
| ScrFI | CCNGG | 1 cut(s) | 561 |
| SetI | ASST | 5 cut(s) | 102, 278, 438, 530, 562 |
| SfaNI | GCATC | 1 cut(s) | 379 |
| SfcI | CTRYAG | 1 cut(s) | 510 |
| SinI | GGWCC | 1 cut(s) | 97 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 50, 206 |
| SmlI | CTYRAG | 1 cut(s) | 467 |
| SmoI | CTYRAG | 1 cut(s) | 467 |
| Sse9I | AATT | 5 cut(s) | 24, 225, 313, 329, 447 |
| SspI | AATATT | 1 cut(s) | 343 |
| SspMI | CTAG | 1 cut(s) | 359 |
| StyD4I | CCNGG | 1 cut(s) | 559 |
| TaaI | ACNGT | 2 cut(s) | 60, 533 |
| TasI | AATT | 5 cut(s) | 24, 225, 313, 329, 447 |
| TatI | WGTACW | 1 cut(s) | 547 |
| TfiI | GAWTC | 1 cut(s) | 211 |
| Tru1I | TTAA | 3 cut(s) | 63, 345, 468 |
| Tru9I | TTAA | 3 cut(s) | 63, 345, 468 |
| TseFI | GTSAC | 2 cut(s) | 54, 523 |
| Tsp45I | GTSAC | 2 cut(s) | 54, 523 |
| TspDTI | ATGAA | 3 cut(s) | 101, 203, 224 |
| Tth111I | GACNNNGTC | 1 cut(s) | 52 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 173 |
| Vha464I | CTTAAG | 1 cut(s) | 467 |
| VpaK11BI | GGWCC | 1 cut(s) | 97 |
| XapI | RAATTY | 1 cut(s) | 313 |
| XceI | RCATGY | 1 cut(s) | 356 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 286 |
| XspI | CTAG | 1 cut(s) | 359 |