RchiOBHm_Chr2g0091931

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001914
Physical Location & Seq
Forward (+)
5573372 .. 5574020
649 bp
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UTR
Exon/CDS
Intron
PRQ46710

Sequence Viewer

Length: 555 bp
ATGTGGAATGGCTTACCGTGGGAGAGATGGTCTTACAAAGTGATCGAAAGACCCTGCTCCGTCACAAAGCCACTACAACAAGCGAGGCTATTTCCGTCACAACGCACAAGTTCCTCTTCCTCTTTACTCTTATATTTCTCTCTCTTCAACCGGCAAAACCCTGAAAACTTAAACCCTGACCGTGAAACTTGTCCGAAGCCGTCCCCGGACCATGTCGTCAACGTGGTCGGCGGAACCTTCGGGGTAGGCTTCGTCTTCGGAGGCGCCCTTCACTTCCTCAAAGGAATCTACAACTCGCCCGGTGGCGCGCGTCTCGCCGGCGCCGCCCAGGCCATCCGTATCAACGCTCCGCTCGTGGGCGGCCGCTTCGCCGTATGGGGCGGCTTATTTTCCACCTTCGACTGCACGTTGGAGCACATGTGGCAAAAAGAGGACCACTGGAACCGGAACATCGCCGGCGCTTCCGCCAGATGCGGCAGGGGTTCGAGCCGACGGCGAGGTCCACCGTCGTGGGAGGGGTACTGTTGGCCCTACTCGAAGGCTTGGAGATCATGA

Protein Analysis

184

Amino Acids

20.59

Weight (kDa)

9.97

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 74 - 155 1.4e-18 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 215, 498
AccB1I GGYRCC 2 cut(s) 263, 320
AccBSI CCGCTC 1 cut(s) 352
AccII CGCG 2 cut(s) 308, 310
AciI CCGC 8 cut(s) 231, 324, 350, 360, 364, 381, 465, 474
AcoI YGGCCR 1 cut(s) 361
AcyI GRCGYC 2 cut(s) 264, 321
AfaI GTAC 1 cut(s) 521
AfiI CCNNNNNNNGG 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 327
AleI CACNNNNGTG 1 cut(s) 508
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 1 cut(s) 317
AoxI GGCC 3 cut(s) 330, 361, 527
AspLEI GCGC 5 cut(s) 266, 308, 310, 323, 461
AspS9I GGNCC 4 cut(s) 208, 433, 500, 528
AsuC2I CCSGG 2 cut(s) 206, 300
AvaII GGWCC 3 cut(s) 208, 433, 500
BanI GGYRCC 2 cut(s) 263, 320
BauI CACGAG 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 417
BccI CCATC 2 cut(s) 21, 341
BceAI ACGGC 3 cut(s) 184, 356, 509
BciT130I CCWGG 1 cut(s) 329
BcnI CCSGG 2 cut(s) 206, 300
BcoDI GTCTC 1 cut(s) 317
BfoI RGCGCY 3 cut(s) 267, 324, 462
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 5 cut(s) 324, 361, 364, 382, 475
BlsI GCNGC 5 cut(s) 325, 362, 365, 383, 476
Bme1390I CCNGG 3 cut(s) 206, 300, 329
Bme18I GGWCC 3 cut(s) 208, 433, 500
BmgT120I GGNCC 4 cut(s) 208, 433, 500, 528
BmiI GGNNCC 4 cut(s) 235, 265, 322, 443
BmrFI CCNGG 3 cut(s) 206, 300, 329
BmsI GCATC 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 247
BpuMI CCSGG 2 cut(s) 206, 300
BsaHI GRCGYC 2 cut(s) 264, 321
BsaJI CCNNGG 3 cut(s) 17, 204, 327
BsaWI WCCGGW 1 cut(s) 444
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 3 cut(s) 150, 317, 455
Bse1I ACTGG 1 cut(s) 443
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 3 cut(s) 17, 204, 327
BseGI GGATG 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 443
BsePI GCGCGC 1 cut(s) 306
BseX3I CGGCCG 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 388
Bsh1236I CGCG 2 cut(s) 308, 310
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 3 cut(s) 332, 363, 529
BshNI GGYRCC 2 cut(s) 263, 320
BsiEI CGRYCG 1 cut(s) 364
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 6 cut(s) 151, 206, 300, 318, 445, 456
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 317
BsmBI CGTCTC 1 cut(s) 317
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 3 cut(s) 332, 363, 529
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 2 cut(s) 42, 548
BspACI CCGC 8 cut(s) 231, 324, 350, 360, 364, 381, 465, 474
BspANI GGCC 3 cut(s) 332, 363, 529
BspFNI CGCG 2 cut(s) 308, 310
BspHI TCATGA 1 cut(s) 551
BspLI GGNNCC 4 cut(s) 235, 265, 322, 443
BspT107I GGYRCC 2 cut(s) 263, 320
BsrBI CCGCTC 1 cut(s) 352
BsrFI RCCGGY 3 cut(s) 150, 317, 455
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 3 cut(s) 150, 317, 455
BssECI CCNNGG 3 cut(s) 17, 204, 327
BssHII GCGCGC 1 cut(s) 306
BssMI GATC 2 cut(s) 42, 548
BssNI GRCGYC 2 cut(s) 264, 321
BssSI CACGAG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 4 cut(s) 18, 182, 507, 524
Bst6I CTCTTC 2 cut(s) 121, 149
BstACI GRCGYC 2 cut(s) 264, 321
BstC8I GCNNGC 3 cut(s) 308, 319, 457
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 1 cut(s) 333
BstFNI CGCG 2 cut(s) 308, 310
BstH2I RGCGCY 3 cut(s) 267, 324, 462
BstHHI GCGC 5 cut(s) 266, 308, 310, 323, 461
BstKTI GATC 2 cut(s) 45, 551
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 2 cut(s) 42, 548
BstMCI CGRYCG 1 cut(s) 364
BstMWI GCNNNNNNNGC 4 cut(s) 314, 323, 329, 421
BstNI CCWGG 1 cut(s) 329
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 3 cut(s) 204, 298, 327
BstUI CGCG 2 cut(s) 308, 310
BstV2I GAAGAC 1 cut(s) 247
BstXI CCANNNNNNTGG 1 cut(s) 510
BstZI CGGCCG 1 cut(s) 361
BsuRI GGCC 3 cut(s) 332, 363, 529
BtgI CCRYGG 1 cut(s) 17
BtgZI GCGATG 1 cut(s) 436
BtsCI GGATG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 308, 319, 457
CciI TCATGA 1 cut(s) 551
CciNI GCGGCCGC 1 cut(s) 361
CfoI GCGC 5 cut(s) 266, 308, 310, 323, 461
Cfr10I RCCGGY 3 cut(s) 150, 317, 455
Cfr13I GGNCC 4 cut(s) 208, 433, 500, 528
CseI GACGC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 520
CviAII CATG 3 cut(s) 212, 418, 552
CviQI GTAC 1 cut(s) 520
DinI GGCGCC 2 cut(s) 265, 322
DpnI GATC 2 cut(s) 44, 550
DpnII GATC 2 cut(s) 42, 548
DrdI GACNNNNNNGTC 2 cut(s) 215, 498
DseDI GACNNNNNNGTC 2 cut(s) 215, 498
EaeI YGGCCR 1 cut(s) 361
EagI CGGCCG 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 121, 149
EarI CTCTTC 2 cut(s) 121, 149
EciI GGCGGA 2 cut(s) 246, 454
EclXI CGGCCG 1 cut(s) 361
Eco47I GGWCC 3 cut(s) 208, 433, 500
Eco52I CGGCCG 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 327
EgeI GGCGCC 2 cut(s) 265, 322
EheI GGCGCC 2 cut(s) 265, 322
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 3 cut(s) 215, 421, 555
FaiI YATR 5 cut(s) 133, 213, 376, 419, 553
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 1 cut(s) 187
FatI CATG 3 cut(s) 211, 417, 551
Fnu4HI GCNGC 5 cut(s) 324, 361, 364, 382, 475
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 5 cut(s) 324, 361, 364, 382, 475
GlaI GCGC 5 cut(s) 265, 307, 309, 322, 460
GluI GCNGC 5 cut(s) 324, 361, 364, 382, 475
HaeII RGCGCY 3 cut(s) 267, 324, 462
HaeIII GGCC 3 cut(s) 332, 363, 529
HapII CCGG 6 cut(s) 151, 206, 300, 318, 445, 456
HgaI GACGC 1 cut(s) 299
HhaI GCGC 5 cut(s) 266, 308, 310, 323, 461
Hin1I GRCGYC 2 cut(s) 264, 321
Hin1II CATG 3 cut(s) 215, 421, 555
Hin6I GCGC 5 cut(s) 264, 306, 308, 321, 459
HinP1I GCGC 5 cut(s) 264, 306, 308, 321, 459
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 1 cut(s) 285
HpaII CCGG 6 cut(s) 151, 206, 300, 318, 445, 456
Hpy166II GTNNAC 2 cut(s) 220, 503
Hpy188I TCNGA 2 cut(s) 195, 260
Hpy188III TCNNGA 1 cut(s) 552
Hpy8I GTNNAC 2 cut(s) 220, 503
Hpy99I CGWCG 2 cut(s) 495, 511
HpyAV CCTTC 4 cut(s) 247, 278, 406, 532
HpyCH4III ACNGT 4 cut(s) 18, 182, 507, 524
HpyCH4IV ACGT 2 cut(s) 222, 407
HpyCH4V TGCA 1 cut(s) 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 323, 329, 421
HpySE526I ACGT 2 cut(s) 222, 407
Hsp92I GRCGYC 2 cut(s) 264, 321
Hsp92II CATG 3 cut(s) 215, 421, 555
HspAI GCGC 5 cut(s) 264, 306, 308, 321, 459
KasI GGCGCC 2 cut(s) 263, 320
KroI GCCGGC 2 cut(s) 317, 455
KroNI GCCGGC 2 cut(s) 319, 457
Kzo9I GATC 2 cut(s) 42, 548
LmnI GCTCC 3 cut(s) 62, 352, 412
LweI GCATC 1 cut(s) 461
MaeII ACGT 2 cut(s) 222, 407
MaeIII GTNAC 2 cut(s) 61, 96
MalI GATC 2 cut(s) 44, 550
MbiI CCGCTC 1 cut(s) 352
MboI GATC 2 cut(s) 42, 548
MboII GAAGA 3 cut(s) 108, 136, 247
MhlI GDGCHC 1 cut(s) 417
Mly113I GGCGCC 2 cut(s) 264, 321
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 8 cut(s) 78, 124, 130, 254, 287, 424, 491, 508
MreI CGCCGGCG 2 cut(s) 317, 455
MroNI GCCGGC 2 cut(s) 317, 455
MseI TTAA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 508
MspI CCGG 6 cut(s) 151, 206, 300, 318, 445, 456
MspR9I CCNGG 3 cut(s) 206, 300, 329
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 2 cut(s) 308, 310
MwoI GCNNNNNNNGC 4 cut(s) 314, 323, 329, 421
NaeI GCCGGC 2 cut(s) 319, 457
NarI GGCGCC 2 cut(s) 264, 321
NciI CCSGG 2 cut(s) 206, 300
NdeII GATC 2 cut(s) 42, 548
NgoMIV GCCGGC 2 cut(s) 317, 455
NlaIII CATG 3 cut(s) 215, 421, 555
NlaIV GGNNCC 4 cut(s) 235, 265, 322, 443
NmuCI GTSAC 2 cut(s) 61, 96
NotI GCGGCCGC 1 cut(s) 361
NspI RCATGY 1 cut(s) 421
OliI CACNNNNGTG 1 cut(s) 508
PagI TCATGA 1 cut(s) 551
PauI GCGCGC 1 cut(s) 306
PciI ACATGT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PdiI GCCGGC 2 cut(s) 319, 457
PfeI GAWTC 1 cut(s) 285
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 5 cut(s) 325, 362, 365, 383, 476
PluTI GGCGCC 2 cut(s) 267, 324
PscI ACATGT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 327
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 4 cut(s) 235, 265, 322, 443
PspPI GGNCC 4 cut(s) 208, 433, 500, 528
PsyI GACNNNGTC 1 cut(s) 212
PteI GCGCGC 1 cut(s) 306
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
RseI CAYNNNNRTG 1 cut(s) 508
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 5 cut(s) 324, 361, 364, 382, 475
Sau3AI GATC 2 cut(s) 42, 548
Sau96I GGNCC 4 cut(s) 208, 433, 500, 528
ScrFI CCNGG 3 cut(s) 206, 300, 329
SduI GDGCHC 1 cut(s) 417
SetI ASST 5 cut(s) 225, 239, 398, 410, 502
SfaNI GCATC 1 cut(s) 461
SfoI GGCGCC 2 cut(s) 265, 322
SgrAI CRCCGGYG 2 cut(s) 317, 455
SinI GGWCC 3 cut(s) 208, 433, 500
SmiMI CAYNNNNRTG 1 cut(s) 508
SsiI CCGC 8 cut(s) 231, 324, 350, 360, 364, 381, 465, 474
SspDI GGCGCC 2 cut(s) 263, 320
StyD4I CCNGG 3 cut(s) 204, 298, 327
TaaI ACNGT 4 cut(s) 18, 182, 507, 524
TaiI ACGT 2 cut(s) 225, 410
TaqI TCGA 4 cut(s) 45, 399, 485, 536
TauI GCSGC 5 cut(s) 326, 363, 366, 384, 477
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TscAI CASTG 1 cut(s) 443
TseFI GTSAC 2 cut(s) 61, 96
Tsp45I GTSAC 2 cut(s) 61, 96
TspGWI ACGGA 3 cut(s) 49, 84, 326
TspRI CASTG 1 cut(s) 443
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 3 cut(s) 208, 433, 500
XceI RCATGY 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.