Rh6AG042000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001914
Physical Location & Seq
Forward (+)
6196410 .. 6196703
294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG042000.1

Sequence Viewer

Length: 294 bp
ATGAAGATTTCGGGTCAGATCGCCCCGAATCAAATCCCTCTGCTACTGAATCCGATTCTGACTCCAGTGCACAGAACAATAGAGATTCAGAGTCTGATTGCGAGAGTGAAACCTGAATCAAATTCTATTGATGAATTTGGGGCTTTCACTGAAAATGGGATGATGTTCAGAGTGAATGATGTGGGTGGGCCTCCTGGTGCAGTTGGGTCTGAAGAAAGCTCCTCCGGTGGGTCCTGGTTGGCAAAGGCAAAGAGCCTGAGCCGGCGAGCAGCGGAAGCGAGAAGAAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.46

Weight (kDa)

9.97

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 272
AcsI RAATTY 2 cut(s) 121, 134
AcuI CTGAAG 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 228
AjnI CCWGG 2 cut(s) 193, 233
AluBI AGCT 1 cut(s) 219
AluI AGCT 1 cut(s) 219
Alw21I GWGCWC 1 cut(s) 72
Alw44I GTGCAC 1 cut(s) 68
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 1 cut(s) 188
ApaLI GTGCAC 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 2 cut(s) 121, 134
AspS9I GGNCC 2 cut(s) 188, 231
AvaII GGWCC 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 281
BciT130I CCWGG 2 cut(s) 195, 235
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 195, 235
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 2 cut(s) 188, 231
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 195, 235
BpmI CTGGAG 1 cut(s) 48
Bpu10I CCTNAGC 1 cut(s) 257
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 65
BseBI CCWGG 2 cut(s) 195, 235
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 219
BshFI GGCC 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 225, 262
BslI CCNNNNNNNGG 1 cut(s) 228
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 1 cut(s) 272
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 261
BssMI GATC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 195, 235
BstC8I GCNNGC 2 cut(s) 263, 267
BstDEI CTNAG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 165
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 2 cut(s) 195, 235
BstSCI CCNGG 2 cut(s) 193, 233
BstSLI GKGCMC 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 281
BsuRI GGCC 1 cut(s) 190
BtsCI GGATG 1 cut(s) 165
BtsIMutI CAGTG 2 cut(s) 72, 147
Cac8I GCNNGC 2 cut(s) 263, 267
CaiI CAGNNNCTG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 2 cut(s) 188, 231
CviJI RGCY 5 cut(s) 143, 190, 219, 255, 261
CviKI_1 RGCY 5 cut(s) 143, 190, 219, 255, 261
DdeI CTNAG 1 cut(s) 257
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 193, 233
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 1 cut(s) 190
HapII CCGG 2 cut(s) 225, 262
HinfI GANTC 7 cut(s) 28, 49, 55, 61, 85, 91, 116
HpaII CCGG 2 cut(s) 225, 262
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 7 cut(s) 18, 54, 60, 90, 96, 170, 211
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 70, 200
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 257
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 1 cut(s) 18
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 9 cut(s) 78, 126, 180, 207, 220, 238, 247, 269, 275
Lsp1109I GCAGC 1 cut(s) 281
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MboII GAAGA 3 cut(s) 16, 224, 294
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 2 cut(s) 121, 134
MlyI GAGTC 2 cut(s) 55, 100
MnlI CCTC 3 cut(s) 48, 201, 232
MroNI GCCGGC 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 2 cut(s) 225, 262
MspR9I CCNGG 2 cut(s) 195, 235
MvaI CCWGG 2 cut(s) 195, 235
MwoI GCNNNNNNNGC 1 cut(s) 275
NaeI GCCGGC 1 cut(s) 263
NdeII GATC 1 cut(s) 18
NgoMIV GCCGGC 1 cut(s) 261
NlaIV GGNNCC 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 263
PfeI GAWTC 5 cut(s) 28, 49, 55, 85, 116
PkrI GCNGC 1 cut(s) 271
PleI GAGTC 2 cut(s) 55, 99
PpsI GAGTC 2 cut(s) 55, 99
PpuMI RGGWCCY 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 193, 233
PspGI CCWGG 2 cut(s) 193, 233
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 2 cut(s) 188, 231
PspPPI RGGWCCY 1 cut(s) 231
PstNI CAGNNNCTG 1 cut(s) 94
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 2 cut(s) 188, 231
SchI GAGTC 2 cut(s) 55, 100
ScrFI CCNGG 2 cut(s) 195, 235
SduI GDGCHC 1 cut(s) 72
SetI ASST 3 cut(s) 115, 221, 290
SinI GGWCC 1 cut(s) 231
Sse9I AATT 2 cut(s) 121, 134
SsiI CCGC 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 193, 233
TasI AATT 2 cut(s) 121, 134
TfiI GAWTC 5 cut(s) 28, 49, 55, 85, 116
TscAI CASTG 2 cut(s) 72, 154
TseI GCWGC 1 cut(s) 269
TspDTI ATGAA 2 cut(s) 17, 147
TspRI CASTG 2 cut(s) 72, 154
VneI GTGCAC 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 2 cut(s) 121, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.