RchiOBHm_Chr5g0014701

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003059
Physical Location & Seq
Forward (+)
10125535 .. 10126191
657 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29515

Sequence Viewer

Length: 657 bp
ATGGAGATTCACGATCTACTGTTTCGGTTCTTAGGATCCGGCCACTTAGACAAGAAATTCGTCACTGACAACGTCATCAGCTTCACCCTCTCCGGAAGAGACACTACCTCGGTTGCGCTGGCATGGTTCTTCTGGCTAATTTCAAACCACCCAAACGTGGATAGCGAAATTCTCAAGGAAATAGAAGAGAAATCGAAGACTGGAGCAAGATATGATGAAGTGAGGGACATGGTGTATACACACGCGGCGCTCTGCAAGAGCATGAGACTGTACCCACCGGTTCCGATCGACAGTAAAGAGGTGGTGATCGACGATGTTTTGTTGAATGGGACTAAAGTGAAAAGGGGGATGGCAGTGCTATACCATGTTTACGCGATGGGGAGGATAGAGCAGATATGGGGCAAGGACTGGGCAGAGTTCCAACCTGAGAAGTGGTTGGAGTGTGGCGTGGCGTGGCGTGGCGTGGCGACAGAGAAATGGAGGCTTGTGGCAAAGGACTCGTTTAACTACCTGTTGTTTCAGGTGGGGCTGAGGATTTGTTTAGGGAAGGAGATGGCGTTCTTGCATATGAAGAGGATGGTGGTTGGGGTTTTGAAGAGGTTTAAGGTGGTACCGGAGAAGAGAGAGAATGGGTTAGAGCCGGATTTTGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

218

Amino Acids

25.2

Weight (kDa)

8.35

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 15 - 208 3.8e-34 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 610
AccB1I GGYRCC 1 cut(s) 610
AccI GTMKAC 1 cut(s) 236
AccII CGCG 2 cut(s) 245, 374
AccIII TCCGGA 1 cut(s) 92
AciI CCGC 1 cut(s) 245
AclWI GGATC 2 cut(s) 30, 43
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 2 cut(s) 56, 168
AfaI GTAC 2 cut(s) 272, 612
AfiI CCNNNNNNNGG 1 cut(s) 157
AgeI ACCGGT 1 cut(s) 277
AgsI TTSAA 3 cut(s) 144, 325, 595
AloI GAACNNNNNNTCC 2 cut(s) 542, 574
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 93, 259
AlwI GGATC 2 cut(s) 30, 43
Aor13HI TCCGGA 1 cut(s) 92
AoxI GGCC 1 cut(s) 40
ApoI RAATTY 2 cut(s) 56, 168
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AsiGI ACCGGT 1 cut(s) 277
Asp718I GGTACC 1 cut(s) 610
AspLEI GCGC 2 cut(s) 118, 250
AsuHPI GGTGA 2 cut(s) 76, 316
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 610
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
BbsI GAAGAC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 530
BccI CCATC 4 cut(s) 343, 370, 547, 571
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BcoDI GTCTC 2 cut(s) 93, 259
BfoI RGCGCY 1 cut(s) 251
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
BmiI GGNNCC 3 cut(s) 37, 282, 612
BmrI ACTGGG 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 222
Bpu10I CCTNAGC 1 cut(s) 530
BpuEI CTTGAG 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 3 cut(s) 92, 277, 613
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 205, 413
BseAI TCCGGA 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 354, 582
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 417, 521
BseNI ACTGG 2 cut(s) 205, 413
Bsh1236I CGCG 2 cut(s) 245, 374
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 42
BshNI GGYRCC 1 cut(s) 610
BshTI ACCGGT 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 288
BsiSI CCGG 5 cut(s) 39, 93, 278, 614, 641
BslFI GGGAC 2 cut(s) 239, 343
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 2 cut(s) 93, 259
BsmFI GGGAC 2 cut(s) 239, 343
BsnI GGCC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 92
Bsp143I GATC 4 cut(s) 13, 35, 285, 306
BspACI CCGC 1 cut(s) 245
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 2 cut(s) 418, 522
BspEI TCCGGA 1 cut(s) 92
BspFNI CGCG 2 cut(s) 245, 374
BspLI GGNNCC 3 cut(s) 37, 282, 612
BspPI GGATC 2 cut(s) 30, 43
BspT107I GGYRCC 1 cut(s) 610
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 2 cut(s) 205, 413
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 4 cut(s) 13, 35, 285, 306
BssNAI GTATAC 1 cut(s) 237
Bst1107I GTATAC 1 cut(s) 237
Bst4CI ACNGT 3 cut(s) 21, 270, 293
Bst6I CTCTTC 5 cut(s) 91, 180, 566, 590, 614
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 5 cut(s) 31, 46, 426, 530, 654
BstF5I GGATG 2 cut(s) 354, 582
BstFNI CGCG 2 cut(s) 245, 374
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 118, 250
BstKTI GATC 4 cut(s) 16, 38, 288, 309
BstMAI GTCTC 2 cut(s) 93, 259
BstMBI GATC 4 cut(s) 13, 35, 285, 306
BstMCI CGRYCG 1 cut(s) 288
BstUI CGCG 2 cut(s) 245, 374
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BstZ17I GTATAC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 42
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 2 cut(s) 354, 582
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 63, 360
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 2 cut(s) 118, 250
Cfr10I RCCGGY 1 cut(s) 277
Csp6I GTAC 2 cut(s) 271, 611
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 4 cut(s) 123, 229, 262, 365
CviJI RGCY 6 cut(s) 42, 81, 136, 484, 529, 640
CviKI_1 RGCY 6 cut(s) 42, 81, 136, 484, 529, 640
CviQI GTAC 2 cut(s) 271, 611
DdeI CTNAG 5 cut(s) 31, 46, 426, 530, 654
DpnI GATC 4 cut(s) 15, 37, 287, 308
DpnII GATC 4 cut(s) 13, 35, 285, 306
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 5 cut(s) 91, 180, 566, 590, 614
EarI CTCTTC 5 cut(s) 91, 180, 566, 590, 614
FaeI CATG 4 cut(s) 126, 232, 265, 368
FaqI GGGAC 2 cut(s) 239, 343
FatI CATG 4 cut(s) 122, 228, 261, 364
FauNDI CATATG 1 cut(s) 567
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 2 cut(s) 361, 589
Fsp4HI GCNGC 1 cut(s) 246
GlaI GCGC 2 cut(s) 117, 249
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 1 cut(s) 42
HapII CCGG 5 cut(s) 39, 93, 278, 614, 641
HhaI GCGC 2 cut(s) 118, 250
Hin1II CATG 4 cut(s) 126, 232, 265, 368
Hin6I GCGC 2 cut(s) 116, 248
HinP1I GCGC 2 cut(s) 116, 248
HinfI GANTC 2 cut(s) 7, 497
HpaII CCGG 5 cut(s) 39, 93, 278, 614, 641
HphI GGTGA 2 cut(s) 76, 316
Hpy166II GTNNAC 2 cut(s) 237, 370
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 2 cut(s) 11, 93
Hpy8I GTNNAC 2 cut(s) 237, 370
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 3 cut(s) 21, 270, 293
HpyCH4IV ACGT 2 cut(s) 72, 156
HpyCH4V TGCA 2 cut(s) 255, 565
HpyF3I CTNAG 5 cut(s) 31, 46, 426, 530, 654
HpySE526I ACGT 2 cut(s) 72, 156
Hsp92II CATG 4 cut(s) 126, 232, 265, 368
HspAI GCGC 2 cut(s) 116, 248
Kpn2I TCCGGA 1 cut(s) 92
KpnI GGTACC 1 cut(s) 614
Kzo9I GATC 4 cut(s) 13, 35, 285, 306
LmnI GCTCC 1 cut(s) 203
MaeII ACGT 2 cut(s) 72, 156
MaeIII GTNAC 1 cut(s) 61
MalI GATC 4 cut(s) 15, 37, 287, 308
MboI GATC 4 cut(s) 13, 35, 285, 306
MboII GAAGA 7 cut(s) 108, 121, 197, 208, 583, 607, 631
MflI RGATCY 1 cut(s) 35
MluCI AATT 3 cut(s) 56, 138, 168
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 2 cut(s) 417, 445
MnlI CCTC 9 cut(s) 98, 118, 216, 292, 375, 474, 525, 567, 591
MroI TCCGGA 1 cut(s) 92
MseI TTAA 2 cut(s) 504, 603
MspI CCGG 5 cut(s) 39, 93, 278, 614, 641
MvnI CGCG 2 cut(s) 245, 374
NdeI CATATG 1 cut(s) 567
NdeII GATC 4 cut(s) 13, 35, 285, 306
NlaIII CATG 4 cut(s) 126, 232, 265, 368
NlaIV GGNNCC 3 cut(s) 37, 282, 612
NmuCI GTSAC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PfeI GAWTC 1 cut(s) 7
PflFI GACNNNGTC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 277
PkrI GCNGC 1 cut(s) 247
Ple19I CGATCG 1 cut(s) 288
PleI GAGTC 1 cut(s) 491
PpsI GAGTC 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 37, 282, 612
PsuI RGATCY 1 cut(s) 35
PsyI GACNNNGTC 1 cut(s) 71
PvuI CGATCG 1 cut(s) 288
RsaI GTAC 2 cut(s) 272, 612
RsaNI GTAC 2 cut(s) 271, 611
SaqAI TTAA 2 cut(s) 504, 603
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 4 cut(s) 13, 35, 285, 306
SchI GAGTC 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 3 cut(s) 56, 138, 168
SsiI CCGC 1 cut(s) 245
TaaI ACNGT 3 cut(s) 21, 270, 293
TaiI ACGT 2 cut(s) 75, 159
TaqI TCGA 3 cut(s) 194, 288, 309
TasI AATT 3 cut(s) 56, 138, 168
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 504, 603
Tru9I TTAA 2 cut(s) 504, 603
TscAI CASTG 2 cut(s) 70, 360
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 2 cut(s) 231, 584
TspRI CASTG 2 cut(s) 70, 360
Tth111I GACNNNGTC 1 cut(s) 71
XapI RAATTY 2 cut(s) 56, 168
XmiI GTMKAC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.