Rroxscaffold_7G00200000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003059
Physical Location & Seq
Forward (+)
47288856 .. 47289869
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200000.1

Sequence Viewer

Length: 567 bp
ATGATATTTTCAATGTCGACGGTGACAACTAGAAATTCCAGCGGCAGTTCTCCAGCCACGAATTCAACACAAAGTCGCTCAAGAAATTCGTGGAAGAATACTACCTTGGATGCTCTAACCTGGATCTTCCGGCTAATCCCAAACCACCCAAACATCGAAAGTAAAATTCTCAAGGAGATCAATGAGAAAAAGGAGAGTGGAGCCGGATATGAGGAAGCCAAGGACATGGTGTACACACAAGCGGCGCTCTGTGAGAGCATGAGGCTGTACTCGCCTGTTCCGATAGAGAGAAAGGAGGCGATCAAAGACGACATTTTGTCGGACAAGGCTAAAGTGAAGAAGAAGATGACGGTGATGTACCACATGTACGCGATGGGGTGGATGGAGCACATATGGGGCAAGGATTTGGCCGAGTTCCGACCGAGAGGTGGTTGGAGAGTGATGTGGCAACGAAGAAGTGGAGGTTTGTGGCGAAGGACTTGTTTAGCTACCCGGTGTTTCGAGCGGGCCGAGGATCTGTCTAGGGAAGGAGATGGAGTTTTTGAAGATGAAAAGGGTGGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

188

Amino Acids

21.61

Weight (kDa)

9.26

Isoelectric Point (pI)

62.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 141 2.6e-15 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 17
AccII CGCG 1 cut(s) 371
AciI CCGC 3 cut(s) 42, 242, 505
AclWI GGATC 2 cut(s) 131, 522
AcoI YGGCCR 1 cut(s) 408
AcsI RAATTY 4 cut(s) 34, 61, 85, 165
AfaI GTAC 4 cut(s) 233, 269, 359, 368
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 3 cut(s) 12, 66, 545
AhdI GACNNNNNGTC 1 cut(s) 316
AjnI CCWGG 1 cut(s) 119
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 1 cut(s) 488
AluI AGCT 1 cut(s) 488
Alw21I GWGCWC 1 cut(s) 390
AlwI GGATC 2 cut(s) 131, 522
AoxI GGCC 2 cut(s) 408, 507
ApoI RAATTY 4 cut(s) 34, 61, 85, 165
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 507
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 34, 364
Bbv12I GWGCWC 1 cut(s) 390
BccI CCATC 3 cut(s) 367, 376, 527
BciT130I CCWGG 1 cut(s) 121
BcnI CCSGG 1 cut(s) 493
BfaI CTAG 2 cut(s) 30, 522
BfoI RGCGCY 1 cut(s) 248
BisI GCNGC 2 cut(s) 43, 243
BlsI GCNGC 2 cut(s) 44, 244
Bme1390I CCNGG 2 cut(s) 121, 493
BmeRI GACNNNNNGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 2 cut(s) 121, 493
BmsI GCATC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 64, 155
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 3 cut(s) 105, 219, 510
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 428
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 3 cut(s) 105, 219, 510
BseGI GGATG 2 cut(s) 115, 387
BseLI CCNNNNNNNGG 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 422
BshFI GGCC 2 cut(s) 410, 509
BsiEI CGRYCG 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 390
BsiSI CCGG 3 cut(s) 130, 204, 493
BslI CCNNNNNNNGG 1 cut(s) 428
BsnI GGCC 2 cut(s) 410, 509
Bsp1286I GDGCHC 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 4 cut(s) 123, 177, 300, 514
BspACI CCGC 3 cut(s) 42, 242, 505
BspANI GGCC 2 cut(s) 410, 509
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 202
BspPI GGATC 2 cut(s) 131, 522
BsrBI CCGCTC 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 231
BssECI CCNNGG 3 cut(s) 105, 219, 510
BssMI GATC 4 cut(s) 123, 177, 300, 514
BssT1I CCWWGG 2 cut(s) 105, 219
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 22, 352
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 507
BstF5I GGATG 2 cut(s) 115, 387
BstFNI CGCG 1 cut(s) 371
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 4 cut(s) 126, 180, 303, 517
BstMBI GATC 4 cut(s) 123, 177, 300, 514
BstMCI CGRYCG 1 cut(s) 422
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNI CCWGG 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 119, 491
BstUI CGCG 1 cut(s) 371
BstX2I RGATCY 2 cut(s) 123, 514
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 2 cut(s) 123, 514
BsuRI GGCC 2 cut(s) 410, 509
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 2 cut(s) 115, 387
Cac8I GCNNGC 1 cut(s) 507
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 507
Csp6I GTAC 4 cut(s) 232, 268, 358, 367
CviAII CATG 3 cut(s) 226, 259, 364
CviQI GTAC 4 cut(s) 232, 268, 358, 367
DpnI GATC 4 cut(s) 125, 179, 302, 516
DpnII GATC 4 cut(s) 123, 177, 300, 514
DriI GACNNNNNGTC 1 cut(s) 316
EaeI YGGCCR 1 cut(s) 408
Eam1105I GACNNNNNGTC 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 105, 219
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 2 cut(s) 105, 219
ErhI CCWWGG 2 cut(s) 105, 219
FaeI CATG 3 cut(s) 229, 262, 367
FaiI YATR 6 cut(s) 210, 227, 260, 365, 392, 394
FatI CATG 3 cut(s) 225, 258, 363
FauI CCCGC 1 cut(s) 498
FauNDI CATATG 1 cut(s) 392
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 43, 243
FokI GGATG 2 cut(s) 122, 394
Fsp4HI GCNGC 2 cut(s) 43, 243
FspBI CTAG 2 cut(s) 30, 522
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 43, 243
GsuI CTGGAG 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 2 cut(s) 410, 509
HapII CCGG 3 cut(s) 130, 204, 493
HhaI GCGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 229, 262, 367
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HpaII CCGG 3 cut(s) 130, 204, 493
HphI GGTGA 2 cut(s) 34, 364
Hpy166II GTNNAC 3 cut(s) 18, 232, 234
Hpy188I TCNGA 3 cut(s) 282, 322, 419
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 3 cut(s) 18, 232, 234
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 2 cut(s) 468, 521
HpyCH4III ACNGT 2 cut(s) 22, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
Hsp92II CATG 3 cut(s) 229, 262, 367
HspAI GCGC 1 cut(s) 245
Kzo9I GATC 4 cut(s) 123, 177, 300, 514
LmnI GCTCC 2 cut(s) 200, 385
LpnPI CCDG 8 cut(s) 52, 66, 106, 133, 143, 217, 288, 506
LweI GCATC 1 cut(s) 100
MaeI CTAG 2 cut(s) 30, 522
MaeIII GTNAC 1 cut(s) 22
MalI GATC 4 cut(s) 125, 179, 302, 516
MbiI CCGCTC 1 cut(s) 505
MboI GATC 4 cut(s) 123, 177, 300, 514
MboII GAAGA 7 cut(s) 106, 118, 349, 352, 355, 465, 557
MflI RGATCY 2 cut(s) 123, 514
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 4 cut(s) 34, 61, 85, 165
MmeI TCCRAC 3 cut(s) 300, 413, 442
MnlI CCTC 6 cut(s) 205, 255, 289, 419, 455, 505
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 3 cut(s) 130, 204, 493
MspR9I CCNGG 2 cut(s) 121, 493
MvaI CCWGG 1 cut(s) 121
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 493
NdeI CATATG 1 cut(s) 392
NdeII GATC 4 cut(s) 123, 177, 300, 514
NlaIII CATG 3 cut(s) 229, 262, 367
NlaIV GGNNCC 1 cut(s) 202
NmeAIII GCCGAG 2 cut(s) 436, 535
NmuCI GTSAC 1 cut(s) 22
NspI RCATGY 1 cut(s) 367
PciI ACATGT 1 cut(s) 363
PcsI WCGNNNNNNNCGW 2 cut(s) 278, 507
PkrI GCNGC 2 cut(s) 44, 244
PscI ACATGT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 1 cut(s) 507
PsuI RGATCY 2 cut(s) 123, 514
RsaI GTAC 4 cut(s) 233, 269, 359, 368
RsaNI GTAC 4 cut(s) 232, 268, 358, 367
SalI GTCGAC 1 cut(s) 16
SatI GCNGC 2 cut(s) 43, 243
Sau3AI GATC 4 cut(s) 123, 177, 300, 514
Sau96I GGNCC 1 cut(s) 507
ScrFI CCNGG 2 cut(s) 121, 493
SduI GDGCHC 1 cut(s) 390
SetI ASST 5 cut(s) 107, 122, 430, 466, 490
SfaNI GCATC 1 cut(s) 100
SmlI CTYRAG 2 cut(s) 79, 170
SmoI CTYRAG 2 cut(s) 79, 170
Sse9I AATT 4 cut(s) 34, 61, 85, 165
SsiI CCGC 3 cut(s) 42, 242, 505
SspMI CTAG 2 cut(s) 30, 522
StyD4I CCNGG 2 cut(s) 119, 491
StyI CCWWGG 2 cut(s) 105, 219
TaaI ACNGT 2 cut(s) 22, 352
TaqI TCGA 3 cut(s) 17, 156, 501
TaqII GACCGA 1 cut(s) 436
TasI AATT 4 cut(s) 34, 61, 85, 165
TatI WGTACW 2 cut(s) 231, 267
TauI GCSGC 2 cut(s) 45, 245
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 564
XapI RAATTY 4 cut(s) 34, 61, 85, 165
XceI RCATGY 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 2 cut(s) 30, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.