Rh6DG311200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003059
Physical Location & Seq
Reverse (-)
51307133 .. 51307863
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG311200.1

Sequence Viewer

Length: 453 bp
ATGGACGAGGAGAAGACTCTGGAGTCTCATGATCTGCTGTCTAGGTTCTTGAGCACCGGCCATTCCGATGAGAAATTCGTCACTGACATCGTCATCAGCTTCACTCTCGCCGGAAGGGACACGACCTCGGCGGCTTTGACCTGGTTCTTCTGGCTAATCTCAAACCATCCAAAAGTCGAAAACCAAATTCTCAAGGAGATCAATGAGAAAACGGAGACCGGAGCCAGATATGATGAAGCGAAGGAGATGGTGTACACACACGTGGCGATCTGTGAGAGCATGAGGCTGTACCCGCCGGTTCCGACTGACAACAAAGAGGCGGTAGAAGACGACGTTTTGCCGGACGGGACTAAAGTGAAGAATGGGACGACAGTGACGTACCACGTGTACGTGATGGGGCGGATGGAGCAGATATGGGGCAAGGATTGGGCCGAGTTCCGTCCCGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

150

Amino Acids

17.32

Weight (kDa)

4.92

Isoelectric Point (pI)

20.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 24 - 150 3e-26 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AciI CCGC 4 cut(s) 131, 293, 320, 400
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 2 cut(s) 74, 186
AcvI CACGTG 2 cut(s) 262, 385
AfaI GTAC 4 cut(s) 254, 290, 380, 389
AflIII ACRYGT 2 cut(s) 259, 384
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 30, 209
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 2 cut(s) 58, 429
ApoI RAATTY 2 cut(s) 74, 186
AspS9I GGNCC 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 443
BbrPI CACGTG 2 cut(s) 262, 385
BbsI GAAGAC 2 cut(s) 20, 333
Bbv12I GWGCWC 1 cut(s) 56
BccI CCATC 4 cut(s) 174, 241, 388, 397
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 30, 209
BfaI CTAG 1 cut(s) 42
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 2 cut(s) 223, 300
BmrFI CCNGG 1 cut(s) 142
BpiI GAAGAC 2 cut(s) 20, 333
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 70, 176
BsaAI YACGTR 3 cut(s) 262, 385, 391
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bse118I RCCGGY 2 cut(s) 56, 295
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 166, 408
BseRI GAGGAG 1 cut(s) 23
BshFI GGCC 2 cut(s) 60, 431
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 5 cut(s) 57, 111, 219, 296, 341
BslFI GGGAC 4 cut(s) 131, 361, 379, 426
BsmAI GTCTC 2 cut(s) 30, 209
BsmFI GGGAC 4 cut(s) 131, 361, 379, 426
BsnI GGCC 2 cut(s) 60, 431
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 3 cut(s) 31, 198, 267
BspACI CCGC 4 cut(s) 131, 293, 320, 400
BspANI GGCC 2 cut(s) 60, 431
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 223, 300
BspTNI GGTCTC 1 cut(s) 209
BsrFI RCCGGY 2 cut(s) 56, 295
BsrGI TGTACA 1 cut(s) 252
BssAI RCCGGY 2 cut(s) 56, 295
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 3 cut(s) 31, 198, 267
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 373
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 3 cut(s) 262, 385, 391
BstF5I GGATG 2 cut(s) 166, 408
BstKTI GATC 3 cut(s) 34, 201, 270
BstMAI GTCTC 2 cut(s) 30, 209
BstMBI GATC 3 cut(s) 31, 198, 267
BstMWI GCNNNNNNNGC 2 cut(s) 292, 406
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstV2I GAAGAC 2 cut(s) 20, 333
BsuRI GGCC 2 cut(s) 60, 431
BtsCI GGATG 2 cut(s) 166, 408
BtsIMutI CAGTG 2 cut(s) 81, 378
CciI TCATGA 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 56, 295
Cfr13I GGNCC 1 cut(s) 429
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 4 cut(s) 253, 289, 379, 388
CviAII CATG 2 cut(s) 29, 280
CviJI RGCY 7 cut(s) 60, 99, 134, 154, 224, 286, 431
CviKI_1 RGCY 7 cut(s) 60, 99, 134, 154, 224, 286, 431
CviQI GTAC 4 cut(s) 253, 289, 379, 388
DpnI GATC 3 cut(s) 33, 200, 269
DpnII GATC 3 cut(s) 31, 198, 267
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 58
EciI GGCGGA 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 209
Eco72I CACGTG 2 cut(s) 262, 385
Eco88I CYCGRG 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 140
FaeI CATG 2 cut(s) 32, 283
FaiI YATR 4 cut(s) 30, 231, 281, 415
FaqI GGGAC 4 cut(s) 131, 361, 379, 426
FatI CATG 2 cut(s) 28, 279
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 2 cut(s) 153, 415
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 42
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 2 cut(s) 60, 431
HapII CCGG 5 cut(s) 57, 111, 219, 296, 341
Hin1II CATG 2 cut(s) 32, 283
HinfI GANTC 2 cut(s) 16, 23
HpaII CCGG 5 cut(s) 57, 111, 219, 296, 341
Hpy166II GTNNAC 3 cut(s) 253, 255, 388
Hpy188I TCNGA 2 cut(s) 67, 303
Hpy188III TCNNGA 4 cut(s) 20, 29, 49, 443
Hpy8I GTNNAC 3 cut(s) 253, 255, 388
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 2 cut(s) 108, 235
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 5 cut(s) 261, 333, 377, 384, 390
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 406
HpySE526I ACGT 5 cut(s) 261, 333, 377, 384, 390
Hsp92II CATG 2 cut(s) 32, 283
Kzo9I GATC 3 cut(s) 31, 198, 267
LmnI GCTCC 2 cut(s) 221, 406
MabI ACCWGGT 1 cut(s) 140
MaeI CTAG 1 cut(s) 42
MaeII ACGT 5 cut(s) 261, 333, 377, 384, 390
MaeIII GTNAC 2 cut(s) 79, 373
MalI GATC 3 cut(s) 33, 200, 269
MboI GATC 3 cut(s) 31, 198, 267
MboII GAAGA 4 cut(s) 25, 139, 338, 370
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 2 cut(s) 74, 186
MlyI GAGTC 2 cut(s) 10, 32
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 4 cut(s) 136, 276, 310, 441
MslI CAYNNNNRTG 2 cut(s) 66, 260
MspI CCGG 5 cut(s) 57, 111, 219, 296, 341
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 292, 406
NdeII GATC 3 cut(s) 31, 198, 267
NlaIII CATG 2 cut(s) 32, 283
NlaIV GGNNCC 2 cut(s) 223, 300
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 2 cut(s) 79, 373
OliI CACNNNNGTG 1 cut(s) 260
PagI TCATGA 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 2 cut(s) 10, 31
PmaCI CACGTG 2 cut(s) 262, 385
PmlI CACGTG 2 cut(s) 262, 385
PpsI GAGTC 2 cut(s) 10, 31
Ppu21I YACGTR 3 cut(s) 262, 385, 391
Psp6I CCWGG 1 cut(s) 140
PspCI CACGTG 2 cut(s) 262, 385
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 223, 300
PspPI GGNCC 1 cut(s) 429
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 4 cut(s) 254, 290, 380, 389
RsaNI GTAC 4 cut(s) 253, 289, 379, 388
RseI CAYNNNNRTG 2 cut(s) 66, 260
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 3 cut(s) 31, 198, 267
Sau96I GGNCC 1 cut(s) 429
SchI GAGTC 2 cut(s) 10, 32
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 56
SexAI ACCWGGT 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 66, 260
SmlI CTYRAG 2 cut(s) 49, 191
SmoI CTYRAG 2 cut(s) 49, 191
Sse9I AATT 2 cut(s) 74, 186
SsiI CCGC 4 cut(s) 131, 293, 320, 400
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 5 cut(s) 264, 336, 380, 387, 393
TaqI TCGA 1 cut(s) 177
TasI AATT 2 cut(s) 74, 186
TatI WGTACW 1 cut(s) 252
TauI GCSGC 1 cut(s) 134
TscAI CASTG 2 cut(s) 88, 378
TseFI GTSAC 2 cut(s) 79, 373
Tsp45I GTSAC 2 cut(s) 79, 373
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 2 cut(s) 227, 428
TspRI CASTG 2 cut(s) 88, 378
Tth111I GACNNNGTC 1 cut(s) 89
XapI RAATTY 2 cut(s) 74, 186
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.