Rh6AG312000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003059
Physical Location & Seq
Reverse (-)
51143420 .. 51143872
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG312000.1

Sequence Viewer

Length: 453 bp
ATGGACGAGAAGAATACTCTGGAGTCTCATGATCTGCTGTCTTGGTTCTTGAGCACCGGCCATTCCGATGAGAAATTCGTCACTGACATCGTCATCAGCTTCACTCTGGCCGGAAGGGACACGACCTCGGCGGCTTTGACCTGGTTCTTCTGGCTAATCTCAAACCATCCAAACGTCGAAAACCAAATTCTTAAGGAGATCAATGAGAAAACGGAGACCGGAGCCAGATATGATGAAGCGAAGGGGATGGTGTACACACACGTGGCGATCTGTGAGAGCATGAGGCTGTACCCGCCGGTTCCGACTGACAACAAAGAGGCGGTAGAAGGCGACGTTTTGCCGGACGGGACTAAAGTGAAGAATGGGACGACAGTGACGTACCACGTGTACGTGATGGGGCGGATGGAGCAGATATGGGGCAAGGATTGGGCCGAGTTCGGTCCCGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

150

Amino Acids

17.09

Weight (kDa)

4.85

Isoelectric Point (pI)

13.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 26 - 150 7.7e-24 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 131, 293, 320, 400
AcoI YGGCCR 2 cut(s) 58, 108
AcsI RAATTY 2 cut(s) 74, 186
AcvI CACGTG 2 cut(s) 262, 385
AfaI GTAC 4 cut(s) 254, 290, 380, 389
AflII CTTAAG 1 cut(s) 191
AflIII ACRYGT 2 cut(s) 259, 384
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 30, 209
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 3 cut(s) 58, 108, 429
ApoI RAATTY 2 cut(s) 74, 186
AspS9I GGNCC 2 cut(s) 429, 440
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 1 cut(s) 440
BbrPI CACGTG 2 cut(s) 262, 385
Bbv12I GWGCWC 1 cut(s) 56
BccI CCATC 4 cut(s) 174, 241, 388, 397
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 30, 209
BfrI CTTAAG 1 cut(s) 191
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 429, 440
BmiI GGNNCC 3 cut(s) 223, 300, 442
BmrFI CCNGG 1 cut(s) 142
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 70
BsaAI YACGTR 3 cut(s) 262, 385, 391
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 218
Bse118I RCCGGY 2 cut(s) 56, 295
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 3 cut(s) 166, 252, 408
BshFI GGCC 3 cut(s) 60, 110, 431
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 5 cut(s) 57, 111, 219, 296, 341
BslFI GGGAC 4 cut(s) 131, 361, 379, 426
BsmAI GTCTC 2 cut(s) 30, 209
BsmFI GGGAC 4 cut(s) 131, 361, 379, 426
BsnI GGCC 3 cut(s) 60, 110, 431
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 3 cut(s) 31, 198, 267
BspACI CCGC 4 cut(s) 131, 293, 320, 400
BspANI GGCC 3 cut(s) 60, 110, 431
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 3 cut(s) 223, 300, 442
BspTI CTTAAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 209
BsrFI RCCGGY 2 cut(s) 56, 295
BsrGI TGTACA 1 cut(s) 252
BssAI RCCGGY 2 cut(s) 56, 295
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 3 cut(s) 31, 198, 267
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 373
BstAFI CTTAAG 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 3 cut(s) 262, 385, 391
BstF5I GGATG 3 cut(s) 166, 252, 408
BstKTI GATC 3 cut(s) 34, 201, 270
BstMAI GTCTC 2 cut(s) 30, 209
BstMBI GATC 3 cut(s) 31, 198, 267
BstMWI GCNNNNNNNGC 2 cut(s) 292, 406
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BsuRI GGCC 3 cut(s) 60, 110, 431
BtsCI GGATG 3 cut(s) 166, 252, 408
BtsIMutI CAGTG 2 cut(s) 81, 378
CciI TCATGA 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 56, 295
Cfr13I GGNCC 2 cut(s) 429, 440
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 4 cut(s) 253, 289, 379, 388
CviAII CATG 2 cut(s) 29, 280
CviJI RGCY 8 cut(s) 60, 99, 110, 134, 154, 224, 286, 431
CviKI_1 RGCY 8 cut(s) 60, 99, 110, 134, 154, 224, 286, 431
CviQI GTAC 4 cut(s) 253, 289, 379, 388
DpnI GATC 3 cut(s) 33, 200, 269
DpnII GATC 3 cut(s) 31, 198, 267
EaeI YGGCCR 2 cut(s) 58, 108
EciI GGCGGA 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 440
Eco72I CACGTG 2 cut(s) 262, 385
Eco88I CYCGRG 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 140
FaeI CATG 2 cut(s) 32, 283
FaiI YATR 4 cut(s) 30, 231, 281, 415
FaqI GGGAC 4 cut(s) 131, 361, 379, 426
FatI CATG 2 cut(s) 28, 279
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 3 cut(s) 153, 259, 415
Fsp4HI GCNGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 3 cut(s) 60, 110, 431
HapII CCGG 5 cut(s) 57, 111, 219, 296, 341
Hin1II CATG 2 cut(s) 32, 283
HinfI GANTC 1 cut(s) 23
HpaII CCGG 5 cut(s) 57, 111, 219, 296, 341
Hpy166II GTNNAC 3 cut(s) 253, 255, 388
Hpy188I TCNGA 2 cut(s) 67, 303
Hpy188III TCNNGA 4 cut(s) 20, 29, 49, 443
Hpy8I GTNNAC 3 cut(s) 253, 255, 388
Hpy99I CGWCG 2 cut(s) 179, 335
HpyAV CCTTC 3 cut(s) 108, 235, 320
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 6 cut(s) 174, 261, 333, 377, 384, 390
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 406
HpySE526I ACGT 6 cut(s) 174, 261, 333, 377, 384, 390
Hsp92II CATG 2 cut(s) 32, 283
Kzo9I GATC 3 cut(s) 31, 198, 267
LmnI GCTCC 2 cut(s) 221, 406
MabI ACCWGGT 1 cut(s) 140
MaeII ACGT 6 cut(s) 174, 261, 333, 377, 384, 390
MaeIII GTNAC 2 cut(s) 79, 373
MalI GATC 3 cut(s) 33, 200, 269
MboI GATC 3 cut(s) 31, 198, 267
MboII GAAGA 3 cut(s) 22, 139, 370
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 2 cut(s) 74, 186
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 4 cut(s) 136, 276, 310, 441
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 66, 260
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 5 cut(s) 57, 111, 219, 296, 341
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 292, 406
NdeII GATC 3 cut(s) 31, 198, 267
NlaIII CATG 2 cut(s) 32, 283
NlaIV GGNNCC 3 cut(s) 223, 300, 442
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 2 cut(s) 79, 373
OliI CACNNNNGTG 1 cut(s) 260
PagI TCATGA 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 31
PmaCI CACGTG 2 cut(s) 262, 385
PmlI CACGTG 2 cut(s) 262, 385
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 3 cut(s) 262, 385, 391
Psp6I CCWGG 1 cut(s) 140
PspCI CACGTG 2 cut(s) 262, 385
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 223, 300, 442
PspPI GGNCC 2 cut(s) 429, 440
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 4 cut(s) 254, 290, 380, 389
RsaNI GTAC 4 cut(s) 253, 289, 379, 388
RseI CAYNNNNRTG 2 cut(s) 66, 260
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 3 cut(s) 31, 198, 267
Sau96I GGNCC 2 cut(s) 429, 440
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 56
SexAI ACCWGGT 1 cut(s) 140
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 2 cut(s) 66, 260
SmlI CTYRAG 2 cut(s) 49, 191
SmoI CTYRAG 2 cut(s) 49, 191
Sse9I AATT 2 cut(s) 74, 186
SsiI CCGC 4 cut(s) 131, 293, 320, 400
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 6 cut(s) 177, 264, 336, 380, 387, 393
TaqI TCGA 1 cut(s) 177
TaqII GACCGA 1 cut(s) 428
TasI AATT 2 cut(s) 74, 186
TatI WGTACW 1 cut(s) 252
TauI GCSGC 1 cut(s) 134
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 2 cut(s) 88, 378
TseFI GTSAC 2 cut(s) 79, 373
Tsp45I GTSAC 2 cut(s) 79, 373
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 2 cut(s) 88, 378
Tth111I GACNNNGTC 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 2 cut(s) 74, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.