Rh5AG441000

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003059
Physical Location & Seq
Reverse (-)
75743794 .. 75749215
5422 bp
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UTR
Exon/CDS
Intron
Rh5AG441000.1

Sequence Viewer

Length: 222 bp
ATGACGGAGAAGCAGGTTATCGTGGTCTTTGAAGTCACGGCGGCCATAGGGCCTTACTGGTCAGCCGTCGTCTCCGATTGCGTCAAGAAGTGGGTGATCGATGAAGAGAATTCTCGGCAGCAGCAATTGCAAACTAGTGGACACGTCATCAGCGGCGGCGGCGGAGAGTGGGTGATCATTGTCGGAAGTGAGTCCTCCCGAGCGGCGAAAAAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

73

Amino Acids

7.96

Weight (kDa)

6.55

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 203
AciI CCGC 6 cut(s) 41, 153, 156, 159, 162, 203
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 109
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 1 cut(s) 32
AhlI ACTAGT 1 cut(s) 134
AjiI CACGTC 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 2 cut(s) 42, 50
ApeKI GCWGC 2 cut(s) 118, 121
ApoI RAATTY 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 106, 184
AvaI CYCGRG 1 cut(s) 198
BbvI GCAGC 2 cut(s) 130, 133
BceAI ACGGC 2 cut(s) 50, 54
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 76
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 4
BisI GCNGC 7 cut(s) 42, 119, 122, 154, 157, 160, 204
BlsI GCNGC 7 cut(s) 43, 120, 123, 155, 158, 161, 205
BmeT110I CYCGRG 1 cut(s) 198
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bse1I ACTGG 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 99
BseNI ACTGG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 130, 133
BshFI GGCC 2 cut(s) 44, 52
BshVI ATCGAT 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 198
BsmAI GTCTC 1 cut(s) 76
BsmBI CGTCTC 1 cut(s) 76
BsnI GGCC 2 cut(s) 44, 52
BsoBI CYCGRG 1 cut(s) 198
Bsp143I GATC 2 cut(s) 96, 174
BspACI CCGC 6 cut(s) 41, 153, 156, 159, 162, 203
BspANI GGCC 2 cut(s) 44, 52
BspDI ATCGAT 1 cut(s) 99
BspMI ACCTGC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 203
BsrI ACTGG 1 cut(s) 62
BssMI GATC 2 cut(s) 96, 174
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 99, 177
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 96, 174
BstMWI GCNNNNNNNGC 2 cut(s) 127, 159
BstV1I GCAGC 2 cut(s) 130, 133
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 2 cut(s) 44, 52
BsuTUI ATCGAT 1 cut(s) 99
BtrI CACGTC 1 cut(s) 145
BveI ACCTGC 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 50
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 1 cut(s) 70
CviJI RGCY 3 cut(s) 44, 52, 65
CviKI_1 RGCY 3 cut(s) 44, 52, 65
DpnI GATC 2 cut(s) 98, 176
DpnII GATC 2 cut(s) 96, 174
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 76
FaiI YATR 2 cut(s) 47, 220
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 7 cut(s) 42, 119, 122, 154, 157, 160, 204
Fsp4HI GCNGC 7 cut(s) 42, 119, 122, 154, 157, 160, 204
FspBI CTAG 1 cut(s) 135
GluI GCNGC 7 cut(s) 42, 119, 122, 154, 157, 160, 204
HaeIII GGCC 2 cut(s) 44, 52
HgaI GACGC 1 cut(s) 70
HinfI GANTC 1 cut(s) 191
HphI GGTGA 2 cut(s) 106, 184
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 2 cut(s) 76, 185
Hpy188III TCNNGA 2 cut(s) 85, 198
Hpy8I GTNNAC 1 cut(s) 140
Hpy99I CGWCG 1 cut(s) 71
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 159
HpySE526I ACGT 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 2 cut(s) 96, 174
LpnPI CCDG 1 cut(s) 43
Lsp1109I GCAGC 2 cut(s) 130, 133
MaeI CTAG 1 cut(s) 135
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 98, 176
MbiI CCGCTC 1 cut(s) 203
MboI GATC 2 cut(s) 96, 174
MboII GAAGA 1 cut(s) 116
MfeI CAATTG 1 cut(s) 125
MluCI AATT 2 cut(s) 109, 125
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 1 cut(s) 205
MspA1I CMGCKG 1 cut(s) 153
MunI CAATTG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 127, 159
NdeII GATC 2 cut(s) 96, 174
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 34
PkrI GCNGC 7 cut(s) 43, 120, 123, 155, 158, 161, 205
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PspPI GGNCC 1 cut(s) 50
SatI GCNGC 7 cut(s) 42, 119, 122, 154, 157, 160, 204
Sau3AI GATC 2 cut(s) 96, 174
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 1 cut(s) 200
SetI ASST 2 cut(s) 18, 147
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 2 cut(s) 109, 125
SsiI CCGC 6 cut(s) 41, 153, 156, 159, 162, 203
SspMI CTAG 1 cut(s) 135
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 109, 125
TauI GCSGC 5 cut(s) 44, 156, 159, 162, 206
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 118, 121
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 1 cut(s) 20
XapI RAATTY 1 cut(s) 109
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.