RLG00000013929

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003059
Physical Location & Seq
Forward (+)
44401179 .. 44401895
717 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013929

Sequence Viewer

Length: 582 bp
ATGGTCCAGCACATCCTTAAGACAAAGTTCCAAATCTACCAAAAGGGCGAGTCCTTTCATACAACTCTCCACGACCTCCTCGGCGACGGCATTTTCAACGCCAGCGCCAAGGACATGGTGTACACACAAGCGGCGCTCTGCGAGAGCGTGCGGCTGTACCCACCGGTTTCGATGGAGAGAAAGGAGGCGATCAACGACGACATTTTGCCGGACGGGACTAAAGTGAAGAAGAGGATGACAGTGATATACCACATGTACGCGATGGGGCGGATGGAGCAGATATGGGGCAAGGATTTGGGCCGAGTTCTGATCCAAGAGGTGGTTGAGCGTGACGTGGCGACGGAGAAGTGGAGGTTCATAGCAAAGGACTCATTTGGCTACCCAACGCTTCAGGCGGGGCTGAGGATCTGTTTTGGGAAGGAGATGTCACTTTTGCAGATGAAAAGGGTGGTGGCTGAGGTTTTGAAGAGGTTTAAGGTTGTGCCGGAGAAGAGGGAGAATGGGAAGGAGCCGGAGTTGCTTGCCTGCCTAACCGTAAAAATGAAAGATGGGTTTCCGGTGACCGTTGTGGAGGGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

194

Amino Acids

22.07

Weight (kDa)

9.03

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 169 1.5e-14 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 319
AccII CGCG 1 cut(s) 260
AciI CCGC 4 cut(s) 131, 151, 268, 395
AclWI GGATC 2 cut(s) 304, 413
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 3 cut(s) 122, 158, 257
AfiI CCNNNNNNNGG 1 cut(s) 319
AflII CTTAAG 1 cut(s) 17
AflIII ACRYGT 1 cut(s) 252
AgeI ACCGGT 1 cut(s) 163
AgsI TTSAA 2 cut(s) 97, 466
AjiI CACGTC 1 cut(s) 334
AlwI GGATC 2 cut(s) 304, 413
AoxI GGCC 1 cut(s) 298
AsiGI ACCGGT 1 cut(s) 163
AspLEI GCGC 2 cut(s) 107, 136
AspS9I GGNCC 2 cut(s) 4, 298
AsuHPI GGTGA 1 cut(s) 571
AvaII GGWCC 1 cut(s) 4
BbvCI CCTCAGC 2 cut(s) 401, 456
BccI CCATC 4 cut(s) 166, 256, 265, 542
BceAI ACGGC 1 cut(s) 103
BfoI RGCGCY 2 cut(s) 108, 137
BfrI CTTAAG 1 cut(s) 17
BisI GCNGC 2 cut(s) 132, 152
BlsI GCNGC 2 cut(s) 133, 153
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 4, 298
BmiI GGNNCC 1 cut(s) 510
Bpu10I CCTNAGC 2 cut(s) 401, 456
BsaJI CCNNGG 2 cut(s) 79, 108
BsaWI WCCGGW 2 cut(s) 163, 556
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 79, 108
BseGI GGATG 3 cut(s) 12, 240, 276
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMII CTCAG 2 cut(s) 392, 447
BseRI GAGGAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 1 cut(s) 300
BshTI ACCGGT 1 cut(s) 163
BsiSI CCGG 5 cut(s) 164, 209, 485, 512, 557
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 1 cut(s) 300
Bsp1407I TGTACA 1 cut(s) 120
Bsp143I GATC 3 cut(s) 189, 309, 405
BspACI CCGC 4 cut(s) 131, 151, 268, 395
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 2 cut(s) 393, 448
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 1 cut(s) 510
BspPI GGATC 2 cut(s) 304, 413
BspTI CTTAAG 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 79, 108
BssMI GATC 3 cut(s) 189, 309, 405
BssT1I CCWWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 241, 535, 565
Bst6I CTCTTC 3 cut(s) 224, 461, 485
BstAFI CTTAAG 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 120
BstC8I GCNNGC 4 cut(s) 103, 149, 522, 526
BstDEI CTNAG 2 cut(s) 401, 456
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 3 cut(s) 12, 240, 276
BstFNI CGCG 1 cut(s) 260
BstH2I RGCGCY 2 cut(s) 108, 137
BstHHI GCGC 2 cut(s) 107, 136
BstKTI GATC 3 cut(s) 192, 312, 408
BstMBI GATC 3 cut(s) 189, 309, 405
BstMWI GCNNNNNNNGC 2 cut(s) 274, 517
BstNSI RCATGY 1 cut(s) 256
BstPI GGTNACC 1 cut(s) 559
BstUI CGCG 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 1 cut(s) 405
BsuRI GGCC 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 275
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 3 cut(s) 12, 240, 276
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 4 cut(s) 103, 149, 522, 526
CfoI GCGC 2 cut(s) 107, 136
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 4, 298
Csp6I GTAC 3 cut(s) 121, 157, 256
CspAI ACCGGT 1 cut(s) 163
CviAII CATG 2 cut(s) 115, 253
CviJI RGCY 7 cut(s) 154, 300, 378, 400, 455, 511, 578
CviKI_1 RGCY 7 cut(s) 154, 300, 378, 400, 455, 511, 578
CviQI GTAC 3 cut(s) 121, 157, 256
DdeI CTNAG 2 cut(s) 401, 456
DpnI GATC 3 cut(s) 191, 311, 407
DpnII GATC 3 cut(s) 189, 309, 405
Eam1104I CTCTTC 3 cut(s) 224, 461, 485
EarI CTCTTC 3 cut(s) 224, 461, 485
EciI GGCGGA 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 374
Eco91I GGTNACC 1 cut(s) 559
EcoO65I GGTNACC 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 2 cut(s) 118, 256
FaiI YATR 6 cut(s) 60, 116, 247, 254, 283, 359
FaqI GGGAC 1 cut(s) 229
FatI CATG 2 cut(s) 114, 252
FauI CCCGC 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 132, 152
FokI GGATG 2 cut(s) 247, 283
Fsp4HI GCNGC 2 cut(s) 132, 152
GlaI GCGC 2 cut(s) 106, 135
GluI GCNGC 2 cut(s) 132, 152
HaeII RGCGCY 2 cut(s) 108, 137
HaeIII GGCC 1 cut(s) 300
HapII CCGG 5 cut(s) 164, 209, 485, 512, 557
HhaI GCGC 2 cut(s) 107, 136
Hin1II CATG 2 cut(s) 118, 256
Hin6I GCGC 2 cut(s) 105, 134
HinP1I GCGC 2 cut(s) 105, 134
HinfI GANTC 2 cut(s) 50, 368
HpaII CCGG 5 cut(s) 164, 209, 485, 512, 557
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 2 cut(s) 121, 123
Hpy188I TCNGA 1 cut(s) 309
Hpy8I GTNNAC 2 cut(s) 121, 123
Hpy99I CGWCG 3 cut(s) 89, 200, 343
HpyAV CCTTC 2 cut(s) 412, 499
HpyCH4III ACNGT 3 cut(s) 241, 535, 565
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 1 cut(s) 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 274, 517
HpyF3I CTNAG 2 cut(s) 401, 456
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 2 cut(s) 118, 256
HspAI GCGC 2 cut(s) 105, 134
Kzo9I GATC 3 cut(s) 189, 309, 405
LmnI GCTCC 2 cut(s) 274, 508
LpnPI CCDG 9 cut(s) 20, 115, 177, 222, 377, 498, 525, 538, 570
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 329, 426, 559
MalI GATC 3 cut(s) 191, 311, 407
MboI GATC 3 cut(s) 189, 309, 405
MboII GAAGA 4 cut(s) 238, 241, 478, 502
MflI RGATCY 1 cut(s) 405
MlyI GAGTC 2 cut(s) 59, 362
MseI TTAA 2 cut(s) 18, 474
MspCI CTTAAG 1 cut(s) 17
MspI CCGG 5 cut(s) 164, 209, 485, 512, 557
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 274, 517
NdeII GATC 3 cut(s) 189, 309, 405
NlaIII CATG 2 cut(s) 118, 256
NlaIV GGNNCC 1 cut(s) 510
NmeAIII GCCGAG 2 cut(s) 60, 326
NmuCI GTSAC 3 cut(s) 329, 426, 559
NspI RCATGY 1 cut(s) 256
PciI ACATGT 1 cut(s) 252
PflMI CCANNNNNTGG 1 cut(s) 319
PinAI ACCGGT 1 cut(s) 163
PkrI GCNGC 2 cut(s) 133, 153
PleI GAGTC 2 cut(s) 58, 362
PpsI GAGTC 2 cut(s) 58, 362
PscI ACATGT 1 cut(s) 252
PspEI GGTNACC 1 cut(s) 559
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 2 cut(s) 4, 298
PsuI RGATCY 1 cut(s) 405
RsaI GTAC 3 cut(s) 122, 158, 257
RsaNI GTAC 3 cut(s) 121, 157, 256
SaqAI TTAA 2 cut(s) 18, 474
SatI GCNGC 2 cut(s) 132, 152
Sau3AI GATC 3 cut(s) 189, 309, 405
Sau96I GGNCC 2 cut(s) 4, 298
SchI GAGTC 2 cut(s) 59, 362
SetI ASST 7 cut(s) 78, 321, 336, 356, 462, 473, 480
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
SsiI CCGC 4 cut(s) 131, 151, 268, 395
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 241, 535, 565
TaiI ACGT 1 cut(s) 336
TaqI TCGA 1 cut(s) 170
TatI WGTACW 1 cut(s) 120
TauI GCSGC 2 cut(s) 134, 154
Tru1I TTAA 2 cut(s) 18, 474
Tru9I TTAA 2 cut(s) 18, 474
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 3 cut(s) 329, 426, 559
Tsp45I GTSAC 3 cut(s) 329, 426, 559
TspDTI ATGAA 4 cut(s) 47, 346, 455, 557
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 246
Van91I CCANNNNNTGG 1 cut(s) 319
Vha464I CTTAAG 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 4
XceI RCATGY 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.