Rmu_sc0000120.1_g000021

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000120.1
OG0003059
Physical Location & Seq
Forward (+)
84313 .. 84834
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000120.1_g000021.1.cds

Sequence Viewer

Length: 522 bp
atggagtttcacgatctgctgtctcggttcttaggatccggccacttagacaagaaattagtcactgacaacgtcatcagcttcaccctctccggaagagacactacctcgattgcactggcatggttcttctggctaatatcaaaccacccaaacgtggataacgaaattctcaaggaaatagaagagaaatcgaagactggagcaagatatgatgaagtgaaggacatggtgtatacacacgcggcgctctgcgagagcatgagactgtacccaccggttccgatcgacagtaaagaggcggtggtcgacgacgttttgctgaatgggactaaagtgaagaaggggatggcagtgctataccatgtttacgcgatggggaggatagagcagatatggggcaaggactgggcaaagttccaacctgagaagtggttggagcgtggcgtggcgtggcgacagagaaatggaggcttgtggcaaaggactcgtttaactacccgttgtttcaggtggggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

173

Amino Acids

20.12

Weight (kDa)

8.85

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 236, 309
AccII CGCG 2 cut(s) 245, 374
AccIII TCCGGA 1 cut(s) 92
AciI CCGC 2 cut(s) 245, 302
AclWI GGATC 2 cut(s) 30, 43
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 1 cut(s) 168
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 157
AgeI ACCGGT 1 cut(s) 277
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw26I GTCTC 3 cut(s) 27, 93, 259
AlwI GGATC 2 cut(s) 30, 43
Aor13HI TCCGGA 1 cut(s) 92
AoxI GGCC 1 cut(s) 40
ApoI RAATTY 1 cut(s) 168
AsiGI ACCGGT 1 cut(s) 277
AspLEI GCGC 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 76
BamHI GGATCC 1 cut(s) 35
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
BbsI GAAGAC 1 cut(s) 203
BccI CCATC 2 cut(s) 343, 370
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BcoDI GTCTC 3 cut(s) 27, 93, 259
BfoI RGCGCY 1 cut(s) 251
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 37, 282
BmrI ACTGGG 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 158
BsaWI WCCGGW 2 cut(s) 92, 277
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 3 cut(s) 123, 205, 413
BseAI TCCGGA 1 cut(s) 92
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 3 cut(s) 123, 205, 413
Bsh1236I CGCG 2 cut(s) 245, 374
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 42
BshTI ACCGGT 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 288
BsiSI CCGG 3 cut(s) 39, 93, 278
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 3 cut(s) 27, 93, 259
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 92
Bsp143I GATC 3 cut(s) 13, 35, 285
BspACI CCGC 2 cut(s) 245, 302
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 1 cut(s) 418
BspEI TCCGGA 1 cut(s) 92
BspFNI CGCG 2 cut(s) 245, 374
BspLI GGNNCC 2 cut(s) 37, 282
BspPI GGATC 2 cut(s) 30, 43
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 3 cut(s) 123, 205, 413
BssAI RCCGGY 1 cut(s) 277
BssMI GATC 3 cut(s) 13, 35, 285
BssNAI GTATAC 1 cut(s) 237
Bst1107I GTATAC 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 270, 293
Bst6I CTCTTC 2 cut(s) 91, 180
BstDEI CTNAG 3 cut(s) 31, 46, 426
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 2 cut(s) 245, 374
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 3 cut(s) 16, 38, 288
BstMAI GTCTC 3 cut(s) 27, 93, 259
BstMBI GATC 3 cut(s) 13, 35, 285
BstMCI CGRYCG 1 cut(s) 288
BstUI CGCG 2 cut(s) 245, 374
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BstZ17I GTATAC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 42
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 3 cut(s) 63, 116, 360
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 277
Csp6I GTAC 1 cut(s) 271
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 4 cut(s) 123, 229, 262, 365
CviJI RGCY 5 cut(s) 42, 81, 136, 474, 519
CviKI_1 RGCY 5 cut(s) 42, 81, 136, 474, 519
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 3 cut(s) 31, 46, 426
DpnI GATC 3 cut(s) 15, 37, 287
DpnII GATC 3 cut(s) 13, 35, 285
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 91, 180
EarI CTCTTC 2 cut(s) 91, 180
FaeI CATG 4 cut(s) 126, 232, 265, 368
FaiI YATR 8 cut(s) 124, 213, 230, 237, 263, 361, 366, 397
FaqI GGGAC 1 cut(s) 343
FatI CATG 4 cut(s) 122, 228, 261, 364
FblI GTMKAC 2 cut(s) 236, 309
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 1 cut(s) 361
Fsp4HI GCNGC 1 cut(s) 246
GlaI GCGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 1 cut(s) 42
HapII CCGG 3 cut(s) 39, 93, 278
HhaI GCGC 1 cut(s) 250
Hin1II CATG 4 cut(s) 126, 232, 265, 368
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 1 cut(s) 487
HpaII CCGG 3 cut(s) 39, 93, 278
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 3 cut(s) 237, 310, 370
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 2 cut(s) 11, 93
Hpy8I GTNNAC 3 cut(s) 237, 310, 370
Hpy99I CGWCG 2 cut(s) 314, 317
HpyAV CCTTC 2 cut(s) 217, 337
HpyCH4III ACNGT 2 cut(s) 270, 293
HpyCH4IV ACGT 3 cut(s) 72, 156, 315
HpyCH4V TGCA 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 31, 46, 426
HpySE526I ACGT 3 cut(s) 72, 156, 315
Hsp92II CATG 4 cut(s) 126, 232, 265, 368
HspAI GCGC 1 cut(s) 248
Kpn2I TCCGGA 1 cut(s) 92
Kzo9I GATC 3 cut(s) 13, 35, 285
LmnI GCTCC 2 cut(s) 203, 439
LpnPI CCDG 9 cut(s) 52, 104, 106, 118, 186, 291, 394, 438, 496
MaeII ACGT 3 cut(s) 72, 156, 315
MaeIII GTNAC 1 cut(s) 61
MalI GATC 3 cut(s) 15, 37, 287
MboI GATC 3 cut(s) 13, 35, 285
MboII GAAGA 5 cut(s) 108, 121, 197, 208, 352
MflI RGATCY 1 cut(s) 35
MluCI AATT 2 cut(s) 56, 168
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 2 cut(s) 417, 445
MnlI CCTC 5 cut(s) 98, 118, 292, 375, 464
MroI TCCGGA 1 cut(s) 92
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 121
MspI CCGG 3 cut(s) 39, 93, 278
MvnI CGCG 2 cut(s) 245, 374
NdeII GATC 3 cut(s) 13, 35, 285
NlaIII CATG 4 cut(s) 126, 232, 265, 368
NlaIV GGNNCC 2 cut(s) 37, 282
NmuCI GTSAC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PflFI GACNNNGTC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 277
PkrI GCNGC 1 cut(s) 247
Ple19I CGATCG 1 cut(s) 288
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 37, 282
PsuI RGATCY 1 cut(s) 35
PsyI GACNNNGTC 1 cut(s) 71
PvuI CGATCG 1 cut(s) 288
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 121
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 3 cut(s) 13, 35, 285
SchI GAGTC 1 cut(s) 481
SetI ASST 7 cut(s) 75, 83, 110, 159, 318, 427, 515
SmiMI CAYNNNNRTG 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 2 cut(s) 56, 168
SsiI CCGC 2 cut(s) 245, 302
TaaI ACNGT 2 cut(s) 270, 293
TaiI ACGT 3 cut(s) 75, 159, 318
TaqI TCGA 4 cut(s) 110, 194, 288, 309
TasI AATT 2 cut(s) 56, 168
TauI GCSGC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 3 cut(s) 70, 123, 360
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 231
TspRI CASTG 3 cut(s) 70, 123, 360
Tth111I GACNNNGTC 1 cut(s) 71
XapI RAATTY 1 cut(s) 168
XmiI GTMKAC 2 cut(s) 236, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.