Rh5BG112500

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003059
Physical Location & Seq
Forward (+)
10538551 .. 10539150
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG112500.1

Sequence Viewer

Length: 600 bp
ATGGACGAGATGAAGACCCTGGAGTCTCACGATCTACTGTGTCGGTTCTTAGGATCCGGCCACTCAGACGAGAAATTCGTCACTGACATCGTCATCAGCTTCACCCTCTCCGGAAGAGACACTACCTCGGTTGCACTGGCCTGGTTCTTCTGGCTAATATCAAACCACCCAAACGTGGATAGCGAAATTCTCAAGGAAATAGAAGAGAAATCGAAGACTGGTGTAGGATATGATGAAGCGAAGGACATGGTGTATACATACGCGGCGCTCTGCGAGAGCATGAGACTGTACCTGCCGGTTCCGATTGACAGTAAAGAGGCGGTGATCGACGACGTTTTGCCGGACGGGACTAAAGTGAAGAAGGGGATGGCAGTGTCATACCATGTTTACGCGATGGGGAGGATAGAGCAGATATGGGGCAAGGACTGGGCAGAGTTCCAACTTGAGAAGTGGTTGGAGCGTGGCGTGGCGACGGAAAAGTGGAGGCTTTTGGCAAAGGACTCGTTTAGCAACCCGGTGTTTCAGGCGGGGTTGGGGATTTGTTTAGGGAAGGAGATGGCGTTCTTGCAGATGAAGAGGATATGGTGGTTGGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

199

Amino Acids

22.77

Weight (kDa)

5.17

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 27 - 192 1.4e-26 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
Acc36I ACCTGC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 254
AccII CGCG 2 cut(s) 263, 392
AccIII TCCGGA 1 cut(s) 110
AciI CCGC 3 cut(s) 263, 320, 527
AclWI GGATC 2 cut(s) 48, 61
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 2 cut(s) 74, 186
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 175
AjnI CCWGG 2 cut(s) 18, 140
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw26I GTCTC 3 cut(s) 30, 111, 277
AlwI GGATC 2 cut(s) 48, 61
Aor13HI TCCGGA 1 cut(s) 110
AoxI GGCC 2 cut(s) 58, 138
ApoI RAATTY 2 cut(s) 74, 186
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 2 cut(s) 94, 334
BamHI GGATCC 1 cut(s) 53
BarI GAAGNNNNNNTAC 2 cut(s) 106, 138
BbsI GAAGAC 2 cut(s) 20, 221
BccI CCATC 3 cut(s) 361, 388, 550
BcgI CGANNNNNNTGC 2 cut(s) 483, 517
BciT130I CCWGG 2 cut(s) 20, 142
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 3 cut(s) 30, 111, 277
BfoI RGCGCY 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 300
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 3 cut(s) 20, 142, 515
BmiI GGNNCC 2 cut(s) 55, 300
BmrFI CCNGG 3 cut(s) 20, 142, 515
BmrI ACTGGG 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 436
BpiI GAAGAC 2 cut(s) 20, 221
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 176, 464
BpuMI CCSGG 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 18, 126
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 3 cut(s) 141, 223, 431
BseAI TCCGGA 1 cut(s) 110
BseBI CCWGG 2 cut(s) 20, 142
BseDI CCNNGG 2 cut(s) 18, 126
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 3 cut(s) 141, 223, 431
Bsh1236I CGCG 2 cut(s) 263, 392
BshFI GGCC 2 cut(s) 60, 140
BsiSI CCGG 5 cut(s) 57, 111, 296, 341, 515
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 3 cut(s) 30, 111, 277
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 2 cut(s) 60, 140
Bsp13I TCCGGA 1 cut(s) 110
Bsp143I GATC 3 cut(s) 31, 53, 324
BspACI CCGC 3 cut(s) 263, 320, 527
BspANI GGCC 2 cut(s) 60, 140
BspCNI CTCAG 1 cut(s) 77
BspEI TCCGGA 1 cut(s) 110
BspFNI CGCG 2 cut(s) 263, 392
BspLI GGNNCC 2 cut(s) 55, 300
BspMI ACCTGC 1 cut(s) 300
BspPI GGATC 2 cut(s) 48, 61
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 3 cut(s) 141, 223, 431
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 18, 126
BssMI GATC 3 cut(s) 31, 53, 324
BssNAI GTATAC 1 cut(s) 255
Bst1107I GTATAC 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 20, 142
Bst4CI ACNGT 3 cut(s) 39, 288, 311
Bst6I CTCTTC 3 cut(s) 109, 198, 569
BstDEI CTNAG 2 cut(s) 49, 64
BstF5I GGATG 1 cut(s) 372
BstFNI CGCG 2 cut(s) 263, 392
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 3 cut(s) 34, 56, 327
BstMAI GTCTC 3 cut(s) 30, 111, 277
BstMBI GATC 3 cut(s) 31, 53, 324
BstNI CCWGG 2 cut(s) 20, 142
BstSCI CCNGG 3 cut(s) 18, 140, 513
BstUI CGCG 2 cut(s) 263, 392
BstV2I GAAGAC 2 cut(s) 20, 221
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BstZ17I GTATAC 1 cut(s) 255
BsuRI GGCC 2 cut(s) 60, 140
BtgZI GCGATG 1 cut(s) 407
BtsCI GGATG 1 cut(s) 372
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 81, 134, 378
BveI ACCTGC 1 cut(s) 300
CfoI GCGC 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 295
Csp6I GTAC 1 cut(s) 289
CviAII CATG 3 cut(s) 247, 280, 383
CviJI RGCY 5 cut(s) 60, 99, 140, 154, 487
CviKI_1 RGCY 5 cut(s) 60, 99, 140, 154, 487
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 2 cut(s) 49, 64
DpnI GATC 3 cut(s) 33, 55, 326
DpnII GATC 3 cut(s) 31, 53, 324
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 3 cut(s) 109, 198, 569
EarI CTCTTC 3 cut(s) 109, 198, 569
EcoRII CCWGG 2 cut(s) 18, 140
FaeI CATG 3 cut(s) 250, 283, 386
FaiI YATR 9 cut(s) 231, 248, 255, 259, 281, 379, 384, 415, 583
FaqI GGGAC 1 cut(s) 361
FatI CATG 3 cut(s) 246, 279, 382
FauI CCCGC 1 cut(s) 520
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 379
Fsp4HI GCNGC 1 cut(s) 264
GlaI GCGC 1 cut(s) 267
GluI GCNGC 1 cut(s) 264
GsuI CTGGAG 1 cut(s) 41
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 2 cut(s) 60, 140
HapII CCGG 5 cut(s) 57, 111, 296, 341, 515
HhaI GCGC 1 cut(s) 268
Hin1II CATG 3 cut(s) 250, 283, 386
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 2 cut(s) 23, 500
HpaII CCGG 5 cut(s) 57, 111, 296, 341, 515
HphI GGTGA 2 cut(s) 94, 334
Hpy166II GTNNAC 2 cut(s) 255, 388
Hpy188I TCNGA 2 cut(s) 67, 303
Hpy188III TCNNGA 2 cut(s) 29, 111
Hpy8I GTNNAC 2 cut(s) 255, 388
Hpy99I CGWCG 3 cut(s) 332, 335, 475
HpyAV CCTTC 3 cut(s) 235, 355, 544
HpyCH4III ACNGT 3 cut(s) 39, 288, 311
HpyCH4IV ACGT 2 cut(s) 174, 333
HpyCH4V TGCA 2 cut(s) 134, 568
HpyF3I CTNAG 2 cut(s) 49, 64
HpySE526I ACGT 2 cut(s) 174, 333
Hsp92II CATG 3 cut(s) 250, 283, 386
HspAI GCGC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 110
Kzo9I GATC 3 cut(s) 31, 53, 324
LmnI GCTCC 1 cut(s) 457
MaeII ACGT 2 cut(s) 174, 333
MaeIII GTNAC 1 cut(s) 79
MalI GATC 3 cut(s) 33, 55, 326
MboI GATC 3 cut(s) 31, 53, 324
MboII GAAGA 7 cut(s) 25, 126, 139, 215, 226, 370, 586
MflI RGATCY 1 cut(s) 53
MluCI AATT 2 cut(s) 74, 186
MlyI GAGTC 2 cut(s) 32, 494
MmeI TCCRAC 2 cut(s) 435, 463
MnlI CCTC 6 cut(s) 116, 136, 310, 393, 477, 570
MroI TCCGGA 1 cut(s) 110
MspI CCGG 5 cut(s) 57, 111, 296, 341, 515
MspR9I CCNGG 3 cut(s) 20, 142, 515
MvaI CCWGG 2 cut(s) 20, 142
MvnI CGCG 2 cut(s) 263, 392
NciI CCSGG 1 cut(s) 515
NdeII GATC 3 cut(s) 31, 53, 324
NlaIII CATG 3 cut(s) 250, 283, 386
NlaIV GGNNCC 2 cut(s) 55, 300
NmuCI GTSAC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 180
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 265
PleI GAGTC 2 cut(s) 31, 494
PpsI GAGTC 2 cut(s) 31, 494
Psp6I CCWGG 2 cut(s) 18, 140
PspGI CCWGG 2 cut(s) 18, 140
PspN4I GGNNCC 2 cut(s) 55, 300
PsuI RGATCY 1 cut(s) 53
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 3 cut(s) 31, 53, 324
SchI GAGTC 2 cut(s) 32, 494
ScrFI CCNGG 3 cut(s) 20, 142, 515
SetI ASST 5 cut(s) 101, 128, 177, 294, 336
SmlI CTYRAG 2 cut(s) 191, 443
SmoI CTYRAG 2 cut(s) 191, 443
Sse9I AATT 2 cut(s) 74, 186
SsiI CCGC 3 cut(s) 263, 320, 527
StyD4I CCNGG 3 cut(s) 18, 140, 513
TaaI ACNGT 3 cut(s) 39, 288, 311
TaiI ACGT 2 cut(s) 177, 336
TaqI TCGA 2 cut(s) 212, 327
TasI AATT 2 cut(s) 74, 186
TauI GCSGC 1 cut(s) 266
TscAI CASTG 3 cut(s) 88, 141, 378
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 3 cut(s) 26, 249, 587
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 3 cut(s) 88, 141, 378
Tth111I GACNNNGTC 1 cut(s) 89
XapI RAATTY 2 cut(s) 74, 186
XmiI GTMKAC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.