RLG00000012942

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003059
Physical Location & Seq
Forward (+)
24168617 .. 24170475
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012942

Sequence Viewer

Length: 399 bp
ATGACGGAGAAGCAGGTTATCTTGGTCTTTGAAGTCACGGCGGCCATAGGGCCTTACTGGATTTCGGTTTCTCTATCTTTGAGTCATTTGATTGCTGTTTACGCTTCAATTGAACTTGATTTTGGGAATTTTGCTTTGTACTTAAACATGCTCGGCGGAGAGTGGGTGATCGATGGAGAGAATTGTTGGCGGCAGCAACTGCAAACTAGTGGACACGTTATCAGCGGCGGCGGAGGAGAGTGGGTGATCGTCGTTGGAAAGGCTTCCTCCTCCCCACCATTGGAAACAGTTGAGCTCTCTCTCTCTCTCAAGTTGGCTTCGACGTCCTCTTTGACTATCTCAAAGAATCAATTACATAAGCATTTAGGTGACACTGGACATGCTTCACTGAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

14.24

Weight (kDa)

5.89

Isoelectric Point (pI)

27.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 4
AciI CCGC 6 cut(s) 41, 156, 190, 225, 228, 231
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 323
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 280
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 3 cut(s) 32, 108, 113
AhlI ACTAGT 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw21I GWGCWC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 199
AoxI GGCC 2 cut(s) 42, 50
ApeKI GCWGC 1 cut(s) 193
ApoI RAATTY 1 cut(s) 127
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
Asp700I GAANNNNTTC 1 cut(s) 262
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 3 cut(s) 178, 256, 380
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 205
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 54
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 1 cut(s) 207
BfuAI ACCTGC 1 cut(s) 4
BisI GCNGC 5 cut(s) 42, 191, 194, 226, 229
BlsI GCNGC 5 cut(s) 43, 192, 195, 227, 230
BmgT120I GGNCC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 323
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 228, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse1I ACTGG 2 cut(s) 62, 379
BseCI ATCGAT 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 280
BseNI ACTGG 2 cut(s) 62, 379
BseRI GAGGAG 2 cut(s) 249, 259
BseXI GCAGC 1 cut(s) 205
BshFI GGCC 2 cut(s) 44, 52
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 297
BslI CCNNNNNNNGG 1 cut(s) 280
BsnI GGCC 2 cut(s) 44, 52
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 168, 246
BspACI CCGC 6 cut(s) 41, 156, 190, 225, 228, 231
BspANI GGCC 2 cut(s) 44, 52
BspDI ATCGAT 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 4
BsrI ACTGG 2 cut(s) 62, 379
BssMI GATC 2 cut(s) 168, 246
BssNI GRCGYC 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 199
BstKTI GATC 2 cut(s) 171, 249
BstMBI GATC 2 cut(s) 168, 246
BstMWI GCNNNNNNNGC 2 cut(s) 101, 199
BstNSI RCATGY 2 cut(s) 151, 383
BstV1I GCAGC 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 2 cut(s) 44, 52
BsuTUI ATCGAT 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 372, 386
BveI ACCTGC 1 cut(s) 4
CaiI CAGNNNCTG 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 50
ClaI ATCGAT 1 cut(s) 171
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 148, 380
CviJI RGCY 5 cut(s) 44, 52, 263, 295, 317
CviKI_1 RGCY 5 cut(s) 44, 52, 263, 295, 317
CviQI GTAC 1 cut(s) 139
DpnI GATC 2 cut(s) 170, 248
DpnII GATC 2 cut(s) 168, 246
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 2 cut(s) 171, 246
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 2 cut(s) 151, 383
FaiI YATR 4 cut(s) 47, 149, 357, 381
FatI CATG 2 cut(s) 147, 379
Fnu4HI GCNGC 5 cut(s) 42, 191, 194, 226, 229
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 5 cut(s) 42, 191, 194, 226, 229
FspBI CTAG 1 cut(s) 207
GluI GCNGC 5 cut(s) 42, 191, 194, 226, 229
HaeIII GGCC 2 cut(s) 44, 52
Hin1I GRCGYC 1 cut(s) 323
Hin1II CATG 2 cut(s) 151, 383
HinfI GANTC 2 cut(s) 82, 346
HphI GGTGA 3 cut(s) 178, 256, 380
Hpy166II GTNNAC 2 cut(s) 100, 212
Hpy8I GTNNAC 2 cut(s) 100, 212
Hpy99I CGWCG 2 cut(s) 254, 325
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 216, 323
HpyCH4V TGCA 1 cut(s) 202
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 199
HpySE526I ACGT 2 cut(s) 216, 323
Hsp92I GRCGYC 1 cut(s) 323
Hsp92II CATG 2 cut(s) 151, 383
Kzo9I GATC 2 cut(s) 168, 246
LpnPI CCDG 2 cut(s) 43, 360
Lsp1109I GCAGC 1 cut(s) 205
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 216, 323
MaeIII GTNAC 2 cut(s) 34, 368
MalI GATC 2 cut(s) 170, 248
MboI GATC 2 cut(s) 168, 246
MfeI CAATTG 1 cut(s) 108
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 4 cut(s) 108, 127, 181, 350
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 5 cut(s) 227, 277, 280, 337, 387
MroXI GAANNNNTTC 1 cut(s) 262
MseI TTAA 1 cut(s) 143
MslI CAYNNNNRTG 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 225
MunI CAATTG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 101, 199
NdeII GATC 2 cut(s) 168, 246
NlaIII CATG 2 cut(s) 151, 383
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 34, 368
NspI RCATGY 2 cut(s) 151, 383
PdmI GAANNNNTTC 1 cut(s) 262
PfeI GAWTC 1 cut(s) 346
PkrI GCNGC 5 cut(s) 43, 192, 195, 227, 230
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 297
PspPI GGNCC 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 199
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 5 cut(s) 42, 191, 194, 226, 229
Sau3AI GATC 2 cut(s) 168, 246
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 297
SetI ASST 6 cut(s) 18, 219, 297, 326, 370, 398
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 4 cut(s) 108, 127, 181, 350
SsiI CCGC 6 cut(s) 41, 156, 190, 225, 228, 231
SspMI CTAG 1 cut(s) 207
SstI GAGCTC 1 cut(s) 297
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 219, 326
TaqI TCGA 2 cut(s) 171, 320
TasI AATT 4 cut(s) 108, 127, 181, 350
TatI WGTACW 1 cut(s) 138
TauI GCSGC 4 cut(s) 44, 193, 228, 231
TfiI GAWTC 1 cut(s) 346
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 2 cut(s) 379, 393
TseFI GTSAC 2 cut(s) 34, 368
TseI GCWGC 1 cut(s) 193
Tsp45I GTSAC 2 cut(s) 34, 368
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 2 cut(s) 379, 393
XapI RAATTY 1 cut(s) 127
XceI RCATGY 2 cut(s) 151, 383
XmnI GAANNNNTTC 1 cut(s) 262
XspI CTAG 1 cut(s) 207
ZraI GACGTC 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.