RLG00000001747

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004872
Physical Location & Seq
Forward (+)
20034258 .. 20037659
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001747

Sequence Viewer

Length: 594 bp
ATGGACGGCCTCTGGCTCTCCCAATGCTACCACAACCACTCTGACATTTACGACGACAGCCGCCGTGCCAGCCCAACAATTTGTCCCGCAAAAAGACCCAGCCCCCTCAGCCCGGCAAGAAGCAGTTCATCATCGAACCCTTTCAAAGGTTTGACCTCGATCCCGAAGCTCGCGTTTGTCACGCAGACAAATATATTGGGGAGCCCTTGGATCGCCGTCATCGCAGCCGTTCTTGTCAGTCTCTGTTACTGTTTCTGTGTAATTGGGCAATGGCGTGCAGGCCTACTTCTCCTACCGAGTAACCACCAAGGTTTGTTGGATATTAGGATCGATAATGAGAGGCCTCCTCAAGGTGGAGAACCCTCAATGCACAATGTCAATTGGAGAGTCAATTACTGCCAGATTCACATATCAAGGCAGAAGCGCCTGTTGGCTGCTCCCACTCGCACTTGTGCTACTCCCTCTTTTTTTCCTAGTTTTGATACTGGATTTTTGGATGTGAGAACTCTAGCTGGAGAACAAGAGAATTGTGATTTTGCTTACAACTATCTTAGTGGTACAATACGAAAGGAATGGGCTTCAAGCTTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.95

Weight (kDa)

8.67

Isoelectric Point (pI)

64.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 173
AciI CCGC 2 cut(s) 61, 87
AclWI GGATC 3 cut(s) 154, 218, 335
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 4 cut(s) 112, 146, 350, 353
AgsI TTSAA 2 cut(s) 145, 582
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 3 cut(s) 169, 512, 585
AluI AGCT 3 cut(s) 169, 512, 585
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 3 cut(s) 154, 218, 335
AlwNI CAGNNNCTG 1 cut(s) 243
AoxI GGCC 3 cut(s) 7, 280, 341
ApeKI GCWGC 2 cut(s) 224, 434
Asp700I GAANNNNTTC 2 cut(s) 124, 140
AspLEI GCGC 1 cut(s) 426
AsuC2I CCSGG 1 cut(s) 113
BanII GRGCYC 1 cut(s) 206
BbvCI CCTCAGC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 236, 421
BceAI ACGGC 4 cut(s) 22, 48, 200, 212
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 2 cut(s) 474, 509
BfoI RGCGCY 1 cut(s) 427
BisI GCNGC 3 cut(s) 61, 225, 435
BlsI GCNGC 3 cut(s) 62, 226, 436
Bme1390I CCNGG 1 cut(s) 113
BmiI GGNNCC 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 113
BplI GAGNNNNNCTC 2 cut(s) 331, 363
BpmI CTGGAG 1 cut(s) 534
Bpu10I CCTNAGC 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 330
BsaJI CCNNGG 2 cut(s) 206, 307
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 146, 350, 353
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 206, 307
BseGI GGATG 1 cut(s) 502
BseLI CCNNNNNNNGG 4 cut(s) 112, 146, 350, 353
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 2 cut(s) 236, 421
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 3 cut(s) 9, 282, 343
BshVI ATCGAT 1 cut(s) 330
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 4 cut(s) 112, 146, 350, 353
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 3 cut(s) 9, 282, 343
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 3 cut(s) 159, 210, 327
BspACI CCGC 2 cut(s) 61, 87
BspANI GGCC 3 cut(s) 9, 282, 343
BspCNI CTCAG 1 cut(s) 120
BspDI ATCGAT 1 cut(s) 330
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 1 cut(s) 203
BspPI GGATC 3 cut(s) 154, 218, 335
BsrDI GCAATG 1 cut(s) 275
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 206, 307
BssMI GATC 3 cut(s) 159, 210, 327
BssT1I CCWWGG 2 cut(s) 206, 307
Bst4CI ACNGT 1 cut(s) 251
BstC8I GCNNGC 5 cut(s) 70, 171, 276, 280, 587
BstDEI CTNAG 2 cut(s) 107, 551
BstENI CCTNNNNNAGG 2 cut(s) 144, 348
BstF5I GGATG 1 cut(s) 502
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 3 cut(s) 162, 213, 330
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 3 cut(s) 159, 210, 327
BstMWI GCNNNNNNNGC 3 cut(s) 69, 108, 221
BstSCI CCNGG 1 cut(s) 111
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 2 cut(s) 236, 421
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 3 cut(s) 9, 282, 343
BsuTUI ATCGAT 1 cut(s) 330
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 502
Cac8I GCNNGC 5 cut(s) 70, 171, 276, 280, 587
CaiI CAGNNNCTG 1 cut(s) 243
CfoI GCGC 1 cut(s) 426
ClaI ATCGAT 1 cut(s) 330
Csp6I GTAC 1 cut(s) 558
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 2 cut(s) 107, 551
DpnI GATC 3 cut(s) 161, 212, 329
DpnII GATC 3 cut(s) 159, 210, 327
Eco130I CCWWGG 2 cut(s) 206, 307
Eco147I AGGCCT 2 cut(s) 282, 343
Eco24I GRGCYC 1 cut(s) 206
EcoNI CCTNNNNNAGG 2 cut(s) 144, 348
EcoT14I CCWWGG 2 cut(s) 206, 307
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 2 cut(s) 206, 307
FaiI YATR 2 cut(s) 194, 410
FaqI GGGAC 1 cut(s) 69
FauI CCCGC 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 61, 225, 435
FokI GGATG 1 cut(s) 509
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 61, 225, 435
FspBI CTAG 2 cut(s) 474, 509
GlaI GCGC 1 cut(s) 425
GluI GCNGC 3 cut(s) 61, 225, 435
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 3 cut(s) 9, 282, 343
HapII CCGG 1 cut(s) 113
HhaI GCGC 1 cut(s) 426
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 583
HinfI GANTC 2 cut(s) 387, 403
HpaII CCGG 1 cut(s) 113
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 163
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 3 cut(s) 278, 370, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 108, 221
HpyF3I CTNAG 2 cut(s) 107, 551
HspAI GCGC 1 cut(s) 424
Kzo9I GATC 3 cut(s) 159, 210, 327
LmnI GCTCC 2 cut(s) 201, 442
LpnPI CCDG 8 cut(s) 82, 112, 126, 264, 413, 440, 471, 498
Lsp1109I GCAGC 2 cut(s) 236, 421
MaeI CTAG 2 cut(s) 474, 509
MaeIII GTNAC 3 cut(s) 178, 245, 299
MalI GATC 3 cut(s) 161, 212, 329
MboI GATC 3 cut(s) 159, 210, 327
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 5 cut(s) 78, 261, 379, 391, 526
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 8 cut(s) 20, 116, 166, 333, 354, 357, 373, 472
MroXI GAANNNNTTC 2 cut(s) 124, 140
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 113
MunI CAATTG 1 cut(s) 379
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 69, 108, 221
NciI CCSGG 1 cut(s) 113
NdeII GATC 3 cut(s) 159, 210, 327
NlaIV GGNNCC 1 cut(s) 203
NmuCI GTSAC 1 cut(s) 178
PceI AGGCCT 2 cut(s) 282, 343
PdmI GAANNNNTTC 2 cut(s) 124, 140
PfeI GAWTC 1 cut(s) 403
PkrI GCNGC 3 cut(s) 62, 226, 436
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PspFI CCCAGC 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 243
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
SatI GCNGC 3 cut(s) 61, 225, 435
Sau3AI GATC 3 cut(s) 159, 210, 327
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 206
SetI ASST 7 cut(s) 151, 158, 171, 313, 355, 514, 587
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
Sse9I AATT 5 cut(s) 78, 261, 379, 391, 526
SseBI AGGCCT 2 cut(s) 282, 343
SsiI CCGC 2 cut(s) 61, 87
SspMI CTAG 2 cut(s) 474, 509
StuI AGGCCT 2 cut(s) 282, 343
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 2 cut(s) 206, 307
TaaI ACNGT 1 cut(s) 251
TaqI TCGA 3 cut(s) 134, 158, 330
TasI AATT 5 cut(s) 78, 261, 379, 391, 526
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 1 cut(s) 403
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 2 cut(s) 224, 434
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 1 cut(s) 117
XagI CCTNNNNNAGG 2 cut(s) 144, 348
XmnI GAANNNNTTC 2 cut(s) 124, 140
XspI CTAG 2 cut(s) 474, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.